Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,263,180 G→T 19.3% D107E (GAC→GAA lolB ← lipoprotein localization factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,263,1800GT19.3% 149.8 / 6.6 75D107E (GAC→GAAlolBlipoprotein localization factor
Reads supporting (aligned to +/- strand):  ref base G (6/53);  new base T (14/2);  total (20/55)
Fisher's exact test for biased strand distribution p-value = 6.83e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAGGTTGCGTTTTGGTGTCATAACCACCATAAACAACCTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGATTGCGCACGTCTTGCTGATGCTGACGCCATTGTGGCGAATCCGGGCTTTTGC  >  NC_000913/1262952‑1263409
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        
tgcgggTTGGGTTTTGGTGTCTTTAACCTAATAAAACATCCTTCCAGTTTTCGTCATTTTGGGTGTAGGGAATTTCGCTCAGGCGGTAGTGGTCGTCCAGTTTGTAGTCGGTTGAATCACCAGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGAAATTGGCATACCGGTAAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAAc                                                                                                                                                                                                                                <  1:572468/235‑1 (MQ=255)
   gggTTGCGTTTTGGGTTTATAACCCCCCAAAACAACCTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCa                                                                                                                                                                                                                            <  1:1171445/238‑1 (MQ=255)
    gggtGCGTTTTGGTGTTATAACCATCATAAACAACCTTCCAGTTTTTGCCATTCTGGCTGTAGGTTATTTCGCTCAGGCGGTACTGGTCGTACAGTTTGTAGTCGGTTGCATCTCCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTACCTGCa                                                                                                                                                                                                                            <  2:785140/235‑1 (MQ=255)
               tttggtactAACCCCCATAACCACCTTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCTAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTGTATTGTCGCCTAACTGCACGTTACCCGGt                                                                                                                                                                                                               <  1:1011458/231‑1 (MQ=255)
                      aTAACCACCATAAACAACCTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCAt                                                                                                                                                                                                        <  2:1129800/239‑1 (MQ=255)
                        atcccccttAAACACCTTTCCAGTTTTTGCCGTTTGGGATGTAGGTAATTCAGCTCAGGGCGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTc                                                                                                                                                                                                      <  1:1068261/231‑1 (MQ=255)
                             cctttatccacCTTTCACTTTTTGCCTTTCTGGTTGTGGGTAATTTCGCTCAGGCGGTACTGGTCGCCCAGTTTGTAGTCGGTTGCATAACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTc                                                                                                                                                                                                 <  1:354386/230‑1 (MQ=255)
                             ccaTAAACATCTTTTCAGTTTTTTCCATTCTGGCTGTAGGTAAGTTCCCCCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTGGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTc                                                                                                                                                                                                 <  1:1019755/239‑1 (MQ=255)
                             ccaTAAACAACCTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTc                                                                                                                                                                                                 <  2:1212863/239‑1 (MQ=255)
                             ccaTAAAATAGCTTTCATTTTTTGCCCTTTTGGCTGTAGGTTATTTCGCCCAGGGCGTACTGGTCGTCCAGTTTGTGGTCGGTTGCATCAACCGGTAAACCTAAAATCCTCTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTc                                                                                                                                                                                                 <  1:638578/239‑1 (MQ=255)
                             cactAAACTACCTTTCGGTTTTTGCCATTTTGGCTGTAGGTAATTCCGGCCAGGCGGTACTGGGCGGCCAGGTTGGAGTCGGTTGCATCACCCGGTAACCCTAAAATCCTCTGGCGCAAGCTGTTTAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTACGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTc                                                                                                                                                                                                 <  1:918056/236‑1 (MQ=255)
                              caTAAACAACCTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGGATAACACAGACCTTTTTTTTTGACCTACTGCCAGTTACCCgcctgggaattaccgccc                                                                                                                                                                                                >  1:1163719/1‑222 (MQ=255)
                                tAAACAACCTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCCGACCCTTTTTTTTGGCTAATCTCACGTTTCCCGGGTGGGCAttacgcccc                                                                                                                                                                                                >  2:1145919/1‑230 (MQ=255)
                                tAAACAACCTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGGATCAAGCTTACCTTTCTTTTTTACTAACTTCCCATTACCCGGTTAAGCAtttcacaccca                                                                                                                                                                                              >  2:8176/1‑230 (MQ=255)
                                  accaacCTTTCATTTTTTGCCATTCTGGCTGTAGGTAATTTCGCGCAGGCGGTACTGGTCGTCCAGTTTTGAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGtt                                                                                                                                                                                            <  1:594297/237‑1 (MQ=255)
                                       ccTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGGCCCTTATTTTCGCCTAAATGCACGTTACCCGGGTGAGCCTTCCGCTCCAGTTCCGt                                                                                                                                                                                        >  2:780596/1‑238 (MQ=255)
                                        cTTCCAGTTTTTGCCATTCTTGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGtgc                                                                                                                                                                                      <  2:947764/239‑1 (MQ=255)
                                        cTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTCACCCTAAAATCCACTGGCTCAAGCTGTTGAGCGGAATTGGGATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTTTAACGCTTCCCCTTCTTTTCGCCCAACTCCACCCTCACCCGTTGAGCATTCGTCCCCAGCTCCGtgc                                                                                                                                                                                      >  1:792386/1‑239 (MQ=255)
                                        cTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGCCCTTTTTTTTCGACTAACTGCACCTTACCCGGTTTAGCATTCACGTCCCGTTCCGtgc                                                                                                                                                                                      >  2:103235/1‑239 (MQ=255)
                                        cTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGtgc                                                                                                                                                                                      <  2:30587/239‑1 (MQ=255)
                                        cTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAAAGCCTACCCTTTTTTTCGACTAAACGCACGTTTCCCCGGTTGGACTTCAGCTCCCGTTCCGt                                                                                                                                                                                        >  2:594297/1‑237 (MQ=255)
                                              gTTTTTTCCATTTGGGGTGTGGGTAATTTCGCCCAGGGGGTACCGGTTGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCACCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCa                                                                                                                                                                                <  1:780596/239‑1 (MQ=255)
                                              gTTTTTGTCTTTTTGTCTTTTTGTATTTTCGCTCAGGGGGTCCTGGTCGGTCCGTTTGTAGTCGGTTGCATGACCCGGTAAACCTAAAATCCTCTGGCGCAAGCTTTTGAGGGGAATTGGCATTCCGGTCAATTTGCCAATCATCGCGTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCa                                                                                                                                                                                <  1:74386/239‑1 (MQ=255)
                                              gTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCa                                                                                                                                                                                <  2:472281/239‑1 (MQ=255)
                                                ttttGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGGCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCa                                                                                                                                                                                <  2:867037/237‑1 (MQ=255)
                                                           tGGCTGTAGGTAATTTCGCTCAGGCGGTACGGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGag                                                                                                                                                                   <  2:179485/239‑1 (MQ=255)
                                                             gCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGGTAGACGCGGGACCTTATTTTTCGCTAACTGCACCGTTCCCCGGTGGGGGATTCACCCCCACTCCGCGCTGcccccaggggttcggtaca                                                                                                                                                                 >  1:37278/1‑223 (MQ=255)
                                                                  tgtttgtttCGCTGAGGCTGTCATGGTCGTCCAGTTTGTAATTGGGTGCTTCACCCGGTAAACCTAAAATCCCCTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCCGCTCTTCGGCGGCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTGGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGgaga                                                                                                                                                            <  1:971502/233‑1 (MQ=255)
                                                                  aGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTGTAAACCTGACCCTTATTTTCTGCCTAATTCCCGTTTACCGCTTGAGCATTCACCCCCCGTTCCCGGCTGCCCCCTTGGGTAGAGagccgacga                                                                                                                                                            >  2:102670/1‑233 (MQ=255)
                                                                  aGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGa                                                                                                                                                            <  2:1237236/239‑1 (MQ=255)
                                                                  aGGTAATTTCGCGCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGa                                                                                                                                                            <  2:881593/239‑1 (MQ=255)
                                                                   tgTAATTTCGCCCAGGCGGTTCTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGa                                                                                                                                                            <  2:105356/237‑1 (MQ=255)
                                                                             cTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACCCCGTGCCTTATTGTCGCACAACTTGCCGTTATCCGGGTGAGCATTCTACTCCACTTTCCTGCCGCCCCACGGGttttggagagcagaacggagggggc                                                                                                                                                  >  1:1254967/1‑215 (MQ=255)
                                                                                            tcgtcCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAAACCTGACCTTTATTTTCGACTAACTGCCCGTTACCCGGTTGAGGCTTCAGCTCCCGTTCCGTGCTGCCCAATGGGGTAGTGAGCAGCGGACGGGAGCGGTCCTGGCCGGGTtgct                                                                                                                                   >  2:1025415/1‑238 (MQ=255)
                                                                                                ccAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCCTTATTTTTGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCCATGGGTTAGTGAGGAGCAGCCGGGAGCGATCCTGGCCGGGTTGGTGcccg                                                                                                                              >  2:824484/1‑237 (MQ=255)
                                                                                                      tGTAGTTGGTTGCTTTCCCCGGTAAACCTAAAACTTACCGGGGCAGGTTTGGGACCGGAATTTGGATTCCGGTCAATTTGCCAATCACCGCTTCGGCGGCATCGTCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAg                                                                                                                        <  1:102670/239‑1 (MQ=255)
                                                                                                      tGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAg                                                                                                                        <  2:37278/239‑1 (MQ=255)
                                                                                                               ttGCCTCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTa                                                                                                                <  2:829429/238‑1 (MQ=255)
                                                                                                                  cATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCGTACCTCTATTGTCCACTTACTGCACGTTACCCGGTTGAGGATTCCTCTCCAGTTCCGTGCTTCCCCATGGGGTATTGAGGAGCAGACGGGAGCGGGCCTGGCCGGTTTGTTGGCAGaaaaaaggggggacacca                                                                                                            >  1:960827/1‑226 (MQ=255)
                                                                                                                       cccTGTAAATCTAAAATCCCCCGGGGCGAGCTTTTGAGCGGAATTGGCATGCCGGTCAATTGGTCAATCATCTCTTCGGCGGCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTttg                                                                                                       <  1:8176/239‑1 (MQ=255)
                                                                                                                        ccGGTAAACCTAAAACCCCCTGGCGCACGTTGTTTGGCGGAATTGGCTTTCCGGTCAATTTGCCAATCATCTCTTCGGGTGCATCGGCGGTATAACGCTGACCTTTAGTGTCGACTAACTGCACGTTACCCGGTTGAGCACTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGTTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTttgt                                                                                                      <  1:669000/239‑1 (MQ=255)
                                                                                                                           gttacccTTAAACCCACTGGCCCAAGTTTTTGAGCGGAATTGGCATTCCGGTAAATTTGCCAATCGTCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTgg                                                                                                   <  1:723259/234‑1 (MQ=255)
                                                                                                                            tAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGGATAACGCTGACCTTTTTTTTCGCCTAAATGCCCGTTCCCCGGTTGAGCATTCAGCTCCAGTTCCGGGCTTCCCCCTTGGTTAGTGTGGAGCAAACGGTAGCGACACCTGCCGGGTTTTTGCCCGCAAAAAAGGGGGGCCACTTTTTTTTGGt                                                                                                  >  1:962184/1‑239 (MQ=255)
                                                                                                                               cccTAAAACCCCCGGGCGCAAGGTTGTGAGCGGAATTGGCATTTCGGTCAATTTGCCAACTCTCTTTTCGGCGGCCTCGGGGGTTAAACGCTGCCCTTTATGTTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAg                                                                                               <  1:1145919/238‑1 (MQ=255)
                                                                                                                               aCCTAAAATTTCCTGGGGCAAGGTGTGGGGCGGAATGGGCATGCCGGTCATTTTGCCAATCATCTCTTCGGCGGCAGCGGCGGTATAACGCTGACCTTTATTGGCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAg                                                                                               <  1:446924/239‑1 (MQ=255)
                                                                                                                               aCCTAAAACCCAGTGCCGTGAGCTGTTGGGCGGAATTGGCATTCCGGTCAATTTGCCAATCCTCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTGCACTTTTTGTTGGTCAg                                                                                               <  1:1084413/239‑1 (MQ=255)
                                                                                                                                ccTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAAAGCCCACCTTTCTTTTCGCCTAACTGCCCCCTTCCCCGCTTAGCATTCAACTCCACTTCCTCGCTGCCCCCTGGGGCTGTGTGCAGGCAACGCTACCGACACCCGCCGGCTTTCTGCCCGAAAACACGGGCGGGCACTTTTTCTTGGTCaca                                                                                              >  1:158729/1‑237 (MQ=255)
                                                                                                                                 cTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTTTCGACTAACTGCACGTTACCCGGTTGAGCATTTCGCTCCCGTTCCGTGCTGCCCAATGGGGTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGGTTGCTGCCCGAAAAAACGGGCGGACACTTTTTGTTGGTCAGaa                                                                                             >  2:237825/1‑239 (MQ=255)
                                                                                                                                 cTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAg                                                                                                                                                      <  2:371481/182‑1 (MQ=255)
                                                                                                                                                     ttgttgggggagaTTGGCTTGCCGGTCAATTTGCCAGATATCTTTTGGGGTTCATCGGAGGTTGAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCGGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGcgccgc                                                                          <  1:590579/226‑1 (MQ=255)
                                                                                                                                                      tGTTGGGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGcgccgc                                                                          <  2:1290444/237‑1 (MQ=255)
                                                                                                                                                      tGTTGGGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGcgccgc                                                                          <  2:323365/237‑1 (MQ=255)
                                                                                                                                                      tGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGcgccgc                                                                          <  2:1018062/237‑1 (MQ=255)
                                                                                                                                                      ggTTGAGCGGAATTGGCTTTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGGCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGa                                                                        <  2:892067/238‑1 (MQ=255)
                                                                                                                                                             cGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGa                                                                  <  2:768623/238‑1 (MQ=255)
                                                                                                                                                              ggAATTGGCTTTCCGGTCAATTTTCCAAATCCTCCTTGGGGGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATa                                                                <  1:1239630/239‑1 (MQ=255)
                                                                                                                                                              ggAATTGGCATTCCGGTCAATTTGCCAATCATCGCTTCGGCGTCATCGGCAGTATAACGTTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATa                                                                <  2:182372/239‑1 (MQ=255)
                                                                                                                                                               taagtggatttCCGGTTAATTTGGCAGTCTGCCTTTCGGAGTCATCGGCGGTTTATCGCGTCCCTTTATTTGCGAGTAACTGCACGTGACCCGGTTGAGCATTCAGCTCGAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTGTGATa                                                                <  1:292065/229‑1 (MQ=255)
                                                                                                                                                               gAATTGGGATTCCGGGCAATTTGCCAATCATTCGTTCGGGGTCATCGGCGGTATAACGCTGACCTTTATTGGCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATa                                                                <  2:960827/238‑1 (MQ=255)
                                                                                                                                                               gAATTGGCATTCCGGTCAATTTGCCAATCATCTTTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGGCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATa                                                                <  2:407674/238‑1 (MQ=255)
                                                                                                                                                               gAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATa                                                                <  2:341844/238‑1 (MQ=255)
                                                                                                                                                               gAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATa                                                                <  2:608964/238‑1 (MQ=255)
                                                                                                                                                               gAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATa                                                                <  2:158729/238‑1 (MQ=255)
                                                                                                                                                               gAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATa                                                                <  2:1163719/238‑1 (MQ=255)
                                                                                                                                                                   tGGCATTCCGGTCAATTTGCCAATCATCTCTGCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGAt                                                           <  2:1222481/239‑1 (MQ=255)
                                                                                                                                                                              tCAATTGGCCAATCATCTCTTCGGCGTCAGCGGTGGTATACCGCTGACCTTTATTGTCGACTAACTGCACGTGCCCCGGTTGAGCAGTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGATTgcg                                                 <  2:967400/238‑1 (MQ=255)
                                                                                                                                                                                 aTTTTCCAATCATCTCTTCGGGGTCTGCGGCGGTTTAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCGCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGATTGCGCACg                                             <  1:679866/239‑1 (MQ=255)
                                                                                                                                                                                          atcatcTCCTCGGCGGCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGATTGCGCACGTCTtgctg                                     <  2:971150/239‑1 (MQ=255)
                                                                                                                                                                                               ctctTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCg                                                                                                                      <  2:227982/153‑1 (MQ=255)
                                                                                                                                                                                                                        cGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGATTGCGCACGTCTTGCTGATGCTGACGCCATTGTGGCGAATCCGGGCt       <  2:862586/239‑1 (MQ=255)
                                                                                                                                                                                                                        cGCTGACCTTTATGGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGATTGCGCACGTCTTGCTGATGCTGACGCCATTGTGGCGAATCCGGGCt       <  2:1108472/239‑1 (MQ=255)
                                                                                                                                                                                                                              aCCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCCATGGGTTAGTGAGCAGCAGACGGTACCGATCCTGGCCGGTTTGCTGCCAGAAAAACCGGGCGTACACTTTTTGTTGTTCAGAAATATAAGCGAACACCCCGCCGGCCTGATATTGATTAAGATTGCGAAAGTCTTGCTGATGACGACTCCATTTGGGGCAATCAGGGCtttt  >  1:800059/1‑237 (MQ=255)
                                                                                                                                                                                                                              aCCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTTGTCAGAAATATAAGCGAACGAGCCGCGAGTCTGATACTGATTAAGATTGCACCCGCCTTGCTGAGTCTGGCGCCATTTTGGCGAAAACAGGCTTTTGc  >  1:511659/1‑239 (MQ=255)
                                                                                                                                                                                                                              aCCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGTTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGa                                                      >  2:713695/1‑187 (MQ=255)
                                                                                                                                                                                                                              aCCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGATTGCGCACGTCTTGCTGATGCTGACGCCATTGTGGCGAATCCGGGCTTTTg   >  1:792075/1‑238 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        
CGCAGGTTGCGTTTTGGTGTCATAACCACCATAAACAACCTTCCAGTTTTTGCCATTCTGGCTGTAGGTAATTTCGCTCAGGCGGTACTGGTCGTCCAGTTTGTAGTCGGTTGCATCACCCGGTAAACCTAAAATCCACTGGCGCAAGCTGTTGAGCGGAATTGGCATTCCGGTCAATTTGCCAATCATCTCTTCGGCGTCATCGGCGGTATAACGCTGACCTTTATTGTCGACTAACTGCACGTTACCCGGTTGAGCATTCAGCTCCAGTTCCGTGCTGCCCAATGGGTTAGTGAGCAGCAGACGGTAGCGATCCTGGCCGGTTTGCTGCCAGAAAAAGCGGGCGTACACTTTTTGTTGGTCAGAAATATAAGCGAACGCGCCGCGAGTCTGATACTGATTAAGATTGCGCACGTCTTGCTGATGCTGACGCCATTGTGGCGAATCCGGGCTTTTGC  >  NC_000913/1262952‑1263409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: