Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,271,857 A→T 16.5% intergenic (‑8/‑262) chaA ← / → chaB calcium/sodium:proton antiporter/cation transport regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,271,8570AT16.5% 134.1 / 2.9 52intergenic (‑8/‑262)chaA/chaBcalcium/sodium:proton antiporter/cation transport regulator
Reads supporting (aligned to +/- strand):  ref base A (7/35);  new base T (7/2);  total (15/37)
Fisher's exact test for biased strand distribution p-value = 7.88e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGACCACACTTAATGTT  >  NC_000913/1271630‑1272095
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
aaCCGAAAGGTTCCCCCAGGTGGTGGGCTAATACGTCCGCAAGACGGAAAACACTAAATGCGCTACTTAAAATCCCAAAAAGGCCAAGAAGATTGATGGCAATGACCACTGGTAGTTGCTGGCTGCTTCCCCACAGGAACAGCACTCCCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAg                                                                                                                                                                                                                                         <  1:814410/239‑1 (MQ=255)
             tcCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTCCGCCCCTTTGGGCATTTGACATTATGGTTTTCCttttcacgtggattttttgg                                                                                                                                                                                                                             >  2:896934/1‑223 (MQ=255)
               ccAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGaaa                                                                                                                                                                                                                         <  2:56727/239‑1 (MQ=255)
               ccAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCCCTACTTAAAATCCCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGaaa                                                                                                                                                                                                                         <  2:675561/239‑1 (MQ=255)
                    cGATGGGCTAATTCGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGGGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAAt                                                                                                                                                                                                                       <  2:304771/236‑1 (MQ=255)
                       tGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTACCGCCCCTTTTGCCTTTGACATTATGGTTTTTCCTTTGCTACTGAAtttttttgaaatattaaa                                                                                                                                                                                                                  >  2:158895/1‑224 (MQ=255)
                                  atgtagaaaagagccagcacTAAAGGCGTTACTTAAAATCACAATCAGCCGAAGAATATTGATGTCAATGACCCCTGGTAGTGTCTGGCCGCTTCCCCACAGGAACAGCACTGCCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAAtaggtagg                                                                                                                                                                                                     <  2:1990/221‑1 (MQ=255)
                                             ggACAACACTAAAGGCGCTACTTAAAATCCCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGa                                                                                                                                                                                         <  2:1147909/239‑1 (MQ=255)
                                               caatcaatAAAGGGTCTATTCAAAAGCCCAATAAGCCCAAAAAAATGAAGGGCACTGACCACTGTTGGGTGCTGTGTGCTCCCCAACCGAAACAGCACTACCAGCGCCAGACCGGGGAAAATAGCCGAAGTCTCCTGGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAAAGTAAAAAATAGGTAGGAAAAATAACAGaa                                                                                                                                                                                        <  1:893480/231‑1 (MQ=255)
                                               acaacaCTAAAGGCGCTACTTAAAATCCCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGaa                                                                                                                                                                                        <  2:193020/238‑1 (MQ=255)
                                                             gcTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTTTCGCCCACTAAGCGATTTACATTATTTTGTTCCCCTTTTAGAGGTATTTCTTTAAAAAATAAAAAAAAATAAAGAAAAATAAAAAAAAAAGTGTGGATAACg                                                                                                                                                                         >  1:902706/1‑239 (MQ=255)
                                                              cTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTCCCCCTCTTGGGCATTTTACACTTTATGTGTTCCTTTTCCGCTTAATTTTTTGACAATGTAAAAAATAGGTAGGATAAAAAAACGaaaaagattggttatatg                                                                                                                                                                        >  2:614856/1‑224 (MQ=255)
                                                                    aaaaTACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTCCTACCCTTCAGCATTTTTCAATATGTTGATTCCTTTTGATTTGGAGTTATTTTAAATAGTAAAAAAAAGGAAGAaaaaaaaaaaaaaaagaatggaaataagggaaat                                                                                                                                                                   >  1:454912/1‑219 (MQ=255)
                                                                         aCCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTATCCGCCCGTTATGCATTTGTCTTTTATTTTTTCCTTTTCTGATTGATTTTTCTACAAAAGTAAAAAAGAGAGTAGAAAAAAAAAAAAAAAatgagataaaatggcaaacattta                                                                                                                                                               >  1:128767/1‑218 (MQ=255)
                                                                          ccAATAAGGCCAAGAAGATTGATGGCAAATCCCCCGGGTAGGGTCTGGCTCCTTCCCCAAAGGAACAGTCCGACCGAGGCCGAAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACg                                                                                                                                                           <  1:351959/239‑1 (MQ=255)
                                                                          ccAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACg                                                                                                                                                           <  2:128767/239‑1 (MQ=255)
                                                                           cAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTCCCCCCCTTTGGCATTTTGCATTATTGTTTTTCCCTTTCGGATGAATTTTTTCCAAATGTGAAAAATAGGGAGAGAAAAAAAAAAAAAAAGTATGGGTATCTGTAACAttttttca                                                                                                                                                          >  2:1201256/1‑235 (MQ=255)
                                                                                                                       tGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTCCGCCCCTTTAGCATTTTACATTATTGTTTTCCCTTTTCCGATGAATTTATTGGAAATGTAAAAAAAAGGTAGGGAAAAAAAAAAAAAATGTCTTGATAATGGTAACAATTTTAGAAATTTTTACCCCCTGTCGGTTTTTTTTTCATTTGGGCGTaaaaa                                                                                                              >  2:261623/1‑239 (MQ=255)
                                                                                                                        ggCTGCTTCCCCACAGAAACAGCACTTCGAGGGCCAAAACCGGGAAAATAAGCGAAGTCTCCTTTTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAAt                                                                                                             <  1:261623/239‑1 (MQ=255)
                                                                                                                             ctTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTTCCGCCCCCTTAGCATTTTGCATTATGGTTATCCCTTTTCAGATGAATTTATCGTAAATTTAAAAAAAAAGTAGGAAAAAAAAAAAAAAAGGTCTGGATATCGGGAAAAATTTTAGAATTTTTACCCCCCCGTCGATTTTTTTTTCCTTTGGGGCTAAAAATaaaaa                                                                                                        >  2:723149/1‑236 (MQ=255)
                                                                                                                              ttCCCCAAAGGAACAGCCATACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCCCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTAt                                                                                                       <  2:454912/239‑1 (MQ=255)
                                                                                                                              ttCCCCAAAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTAt                                                                                                       <  2:1172510/239‑1 (MQ=255)
                                                                                                                              ttCCCCAAAGGAAAAGCCTTCCCGGAGCAAGAACCGGAAAAATAAGGGAATTTTCCTTGTGGCGGTTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTAt                                                                                                       <  1:896934/239‑1 (MQ=255)
                                                                                                                              tcccccccAGGAACAGCACTACCAGGCCCAGAACCGGGAAAATAAGGGAAGTCTCCTTGTGGGGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTAt                                                                                                       <  1:158895/237‑1 (MQ=255)
                                                                                                                                               cctcccAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGTTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAAtgt                                                                                       <  2:353658/234‑1 (MQ=255)
                                                                                                                                               aCTACCAGCGCCAGACCCGGGAAAATAAGCGAAGTCTCCTTGGGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAAtgt                                                                                       <  2:418568/238‑1 (MQ=255)
                                                                                                                                                  aCCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGGTTTTTCCGCCCCTTTAGCATTTTACATTATTGGTTTTCCTTTTCAGATGAATTTTTTGCAAAATTAAAAAATAAGTAGGAAAAAAAACAAAAAATGTCTGGATATCGGTAAAATTTTTAGAAATTTTTACCCGCTGTCGATTTTTTTCTCATTTGGGGGTTAAAATAAGAATTAAGGTTTGACAAtgtttgt                                                                                   >  2:162804/1‑239 (MQ=255)
                                                                                                                                                          gggagaggtgtataagggacaggTCTCCTTGGGGCGGGTTTTTACCGCCTATTGAGCATTTGAAATTATGGTTATCCCTTTGCAGATGAATTTACCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                           <  2:1090081/217‑1 (MQ=255)
                                                                                                                                                          caaacctgggAAAATAAGGGAAGTCTCCTTGTGGGGGGTTTTTTCCGCCTTTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGGAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                           <  1:723149/232‑1 (MQ=255)
                                                                                                                                                          caaaaCTGGTAATATAAGCGAGTCTCTGTTTTGGCTGTTTTTTCCCGCATTTTGAGCATTTGACATTATGGTTTTCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                           <  1:814188/236‑1 (MQ=255)
                                                                                                                                                          cAGAACCTGGAAAATTAGCGAATTCTCTTTGTGCCGGGTTTTTACCGCCTCTTGAGCATTTGAAATTATGGTTATCCCATTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                           <  1:115791/239‑1 (MQ=255)
                                                                                                                                                          cAGAACCGGTAAAATAAGCGAAGTCTCCTGTGGGCGGGTTTTTACCGCCTTTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                           <  2:828130/239‑1 (MQ=255)
                                                                                                                                                          cAGAACCGGGAAAATAAGGTAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAACGTTTGTTTTAGCGt                                                                           <  1:676501/239‑1 (MQ=255)
                                                                                                                                                                    aaaaTAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTaaga                                                                  <  2:902706/238‑1 (MQ=255)
                                                                                                                                                                                  tttgttttggggggttttttGCCGTCTGTTGAGCATTTGACCTTATTGTTATCCCGTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGTAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTGTAGCGTCTCTTAAGAAGAGTCTGACCTGa                                                    <  1:614856/224‑1 (MQ=255)
                                                                                                                                                                                   cttcTTGTGGCGTGTTTTTACCGCTTTTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGaa                                                   <  1:1155123/236‑1 (MQ=255)
                                                                                                                                                                                                ttttttACCGCCTGTTGAGAATTTGACATTAGTGTTACCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGtt                                       <  1:917198/237‑1 (MQ=255)
                                                                                                                                                                                                    ttACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGc                                  <  2:596229/239‑1 (MQ=255)
                                                                                                                                                                                                     tACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCa                                 <  2:1098583/239‑1 (MQ=255)
                                                                                                                                                                                                           ccTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGat                                                                                                                                                                             >  2:279643/1‑95 (MQ=255)
                                                                                                                                                                                                           ccTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGat                                                                                                                                                                             <  1:279643/95‑1 (MQ=255)
                                                                                                                                                                                                                ttGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTAt                                                                                                       >  1:362716/1‑160 (MQ=255)
                                                                                                                                                                                                                ttGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTAt                                                                                                       <  2:362716/160‑1 (MQ=255)
                                                                                                                                                                                                                      aTTTGACATTATGGTTAACCCTTTGCAGATGAATTTATCGAAAATGCAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATAttgtt                  <  2:1102029/239‑1 (MQ=255)
                                                                                                                                                                                                                        ttGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATAtttttg                 >  2:856421/1‑238 (MQ=255)
                                                                                                                                                                                                                        ttGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGAAATTGTTGa                >  2:637132/1‑239 (MQ=255)
                                                                                                                                                                                                                         ataCATTTTGGTTTACCCTTTGCAGGTGAATTTAACGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATTTCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGAc               <  1:953426/237‑1 (MQ=255)
                                                                                                                                                                                                                          gACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGGAGGAAAAATAACAGAAAGTGTCTGGATAGCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGAcc              <  1:856421/239‑1 (MQ=255)
                                                                                                                                                                                                                              ttATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGAc               <  2:24163/234‑1 (MQ=255)
                                                                                                                                                                                                                               tATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGACCACACt         <  2:845774/239‑1 (MQ=255)
                                                                                                                                                                                                                               tATGGTTATCCCTTTGCAGATGAATTGAACGAAAAGTTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTTGATATCGGTAACATTTTACGAATTTTTGCCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGACCACACt         <  2:1190145/239‑1 (MQ=255)
                                                                                                                                                                                                                                  ggTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGAATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTTTGTTTTGGCAAAAGTAAAAATTTTTGACCAAACTaaa      >  1:480445/1‑239 (MQ=255)
                                                                                                                                                                                                                                      aTCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGc                                                                                                                                             >  1:833481/1‑100 (MQ=255)
                                                                                                                                                                                                                                      aTCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGc                                                                                                                                             <  2:833481/100‑1 (MQ=255)
                                                                                                                                                                                                                                      aTCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                           >  2:174202/1‑166 (MQ=255)
                                                                                                                                                                                                                                      aTCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                           >  2:1314692/1‑166 (MQ=255)
                                                                                                                                                                                                                                      aTCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                           <  1:174202/166‑1 (MQ=255)
                                                                                                                                                                                                                                      aTCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGt                                                                           <  1:1314692/166‑1 (MQ=255)
                                                                                                                                                                                                                                      aTCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGTTATTGTTGACCACACTTAAt     >  2:953426/1‑236 (MQ=255)
                                                                                                                                                                                                                                      aTCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGACCACACTTAATGt   >  2:338698/1‑238 (MQ=255)
                                                                                                                                                                                                                                      aTCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTAACCACAATAAAAGtt  >  1:1202639/1‑239 (MQ=255)
                                                                                                                                                                                                                                      aTCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGCAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTAGGTTTGAACATGGTTTGTTTTAGCGTGTCTTACGAAAATTTTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATAATGTGTCCCCCACTTAATGtt  >  1:1190145/1‑239 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  
AACCGTAAGGTTCTCCCAGGCGATGGGCTAATACGTCCGCATGACGGACAACACTAAAGGCGCTACTTAAAATACCAATAAGCGCAAGAAGATTGATGGCAATGACCACTGGTAGTGTCTGGCTGCTTCCCCACAGGAACAGCACTACCAGCGCCAGAACCGGGAAAATAAGCGAAGTCTCCTTGTGGCGGGTTTTTACCGCCTCTTGAGCATTTGACATTATGGTTATCCCTTTGCAGATGAATTTATCGAAAATGTAAAAAATAGGTAGGAAAAATAACAGAAAGTGTCTGGATATCGGTAACATTTTACGAATTTTTACCCCGCTGTCGATTTTTTACTCATTGGGGCATAAAAATAAGTATTACGTTTAGACAATGTTTGTTTTAGCGTCTCTTAAGAAGAGTCTGACCTGAAAATTCTTATGTTTTGGCAAGAGTAGATATTGTTGACCACACTTAATGTT  >  NC_000913/1271630‑1272095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: