Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,464,046	G→T	12.8%	E170* (GAA→TAA)	paaX →	transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,464,046	0	G	T	12.8%	228.9 / 2.2	97	E170* (GAA→TAA)	paaX	transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer
Reads supporting (aligned to +/- strand):  ref base G (19/64);  new base T (12/2);  total (31/66)
Fisher's exact test for biased strand distribution p-value = 1.13e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTGTCGTCCTTAAAGACCCATTGTTGCCGGAG  >  NC_000913/1463810‑1464282                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                cgggaggggCAAAAAGTAAAATTTTTCGCGCGGGGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTTTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGa                                                                                                                                                                                                                                                <  1:851754/232‑1 (MQ=255) cgcgACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGa                                                                                                                                                                                                                                                <  2:1261468/238‑1 (MQ=255)  gcgACGGGCAGAAAGTAAAATTGATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAg                                                                                                                                                                                                                                              <  2:149418/239‑1 (MQ=255)  ccgccGGGCGAAAAGTAATTTTTGTCGCGTAGAGCCACCTGCATGGGATGGTAAATGGCGTCTGTTGCTGTCGGTATGTTTAGATAATCCAAGGTTGGTGGTGGTCAGAAGGTA‑GTTGATCTGGCAAGGTTTGGGCTAACTGGCACCCC‑GGCTGATGGCATCGCCGTCGCAAAAACTTGCCGATGTACAGACTCTTG‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTGTTGaa                                                                                                                                                                                                                                               <  1:707840/234‑1 (MQ=255)         gCAGAAAGTAAAATTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAAGCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑aaa                                                                                                                                                                                                                                       <  2:1063167/238‑1 (MQ=255)                   aaaTTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGgcg                                                                                                                                                                                                                            <  2:1166784/239‑1 (MQ=255)                        tttcctccatagCTACCTGCATTGGGTGGTAAAGGGCTCTTGTTGCTCTGGGAAGGTTTAGATAAATCAACGCTTGCTGATGGCAAAAGGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCCCCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTtc                                                                                                                                                                                                                       <  1:654112/229‑1 (MQ=255)                        tATCGCGCAGAGCACCCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTtc                                                                                                                                                                                                                       <  2:200188/239‑1 (MQ=255)                        tATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTtc                                                                                                                                                                                                                       <  2:859674/239‑1 (MQ=255)                         aTCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTCCAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAAAGTGATTTGTTTTGAAACGC‑AAAAACCACTGGCGCtttt                                                                                                                                                                                                                       >  2:103718/1‑237 (MQ=255)                         aTCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGGTAACGTGAATTTTTTTTAAGGGCAAAATACC‑CTGGTGCtttt                                                                                                                                                                                                                       >  2:419426/1‑237 (MQ=255)                           cgcgCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTTAAGCGC‑AAAAACCACTGGGGCCTtttc                                                                                                                                                                                                                     >  2:162186/1‑238 (MQ=255)                           cgcgCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAAAGTGATTTGTTTTTAAGCGC‑CAAAACCACCgggcgtttc                                                                                                                                                                                                                       >  2:1011781/1‑229 (MQ=255)                           cgcgCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGGGCCGGATAAAGGGATTTGTTTTGAAGCGC‑AAATACCCCTGGCGCGTtttc                                                                                                                                                                                                                     >  2:1211877/1‑238 (MQ=255)                           cgcgCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCCCAAAAACTGGCCGCTGTACAGACACTTT‑TG‑CATGAACAGGGGGAGGCGGCAAAAGTGGTTATTTTTTAATCGC‑AAATAcaccccgggctgtccc                                                                                                                                                                                                                     >  1:62765/1‑223 (MQ=255)                                  agCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGGTAACGTGATTTGTTTTGGAGCGC‑AAAAACCACTGGCGCTTTTTCGcgccg‑c                                                                                                                                                                                                             >  2:212060/1‑238 (MQ=255)                                  agCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGGTGGGCGGTGAACGTGATTTGTTTTGAAGCGC‑AAATACCACTTGGCCTTTTTcgccccgcc                                                                                                                                                                                                             >  2:415748/1‑233 (MQ=255)                                   gCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCgcag                                                                                                                                                                                                               <  2:681341/236‑1 (MQ=255)                                          tcATGGGATGGTAATTGGCTTCGTTTACTCTCGGAAGGTTTAGATAAATCTACCCTTGCCTGTTTCAAAAAGCA‑GTTTATCTTGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAGCTTGCCGATGTACAGACCCTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAGGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGc                                                                                                                                                                                                    <  1:109907/238‑1 (MQ=255)                                          gCATGGGATGGTTAATGGCTTCTTTTGCTCGCGGAAGTTTTAGATAAAGAGACTGTGGCTGAAGTCAAAAAGGATGGTG‑TCTGGCAAGGTTTTGGCGCACTGGCTCCCA‑GCCTGAGGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTTAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGc                                                                                                                                                                                                    <  1:1217002/239‑1 (MQ=255)                                           aaTGGGATGGTAAATGGTCCCTGTTGCTGGCGGAAGGTTTAGAGAAATCAAGGCGGGCGGATGTCAAAAAGCA‑GTTGATCTGGCAAGGGTTTGGCGCACTGGCACCCA‑GCCTGATGGCAGCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGc                                                                                                                                                                                                    <  1:220236/237‑1 (MQ=255)                                            aTGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGGTAACGGTGGTTGTTTTGTATCGC‑AAAAAACCCTTGCGCCTtcttccgccagaatgcggggcc                                                                                                                                                                                                   >  2:501219/1‑220 (MQ=255)                                            aTGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCa                                                                                                                                                                                                  <  2:1252936/239‑1 (MQ=255)                                            aTGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGGGATTTGTTTTTAAGGGC‑AAAAACCACTGGGGCCTTTTAGGGCAG‑ACCTGCggggca                                                                                                                                                                                                  >  2:455810/1‑234 (MQ=255)                                            aTGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGAAAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCa                                                                                                                                                                                                  <  1:69100/239‑1 (MQ=255)                                             tGGGATGGTAAATGGCTCCTGTTGATCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCa                                                                                                                                                                                                  <  2:1318353/238‑1 (MQ=255)                                                gATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGaa                                                                                                                                                                                                                                               <  2:153269/192‑1 (MQ=255)                                                gATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTtc                                                                                                                                                                                                                       <  2:1311652/215‑1 (MQ=255)                                                gATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCagag                                                                                                                                                                                               <  2:867458/238‑1 (MQ=255)                                                   ggTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGTCAACGGGAGTTGTTTTGAAGCGG‑AAAAAACACGGGGGCTTTCTCGGGCCG‑CACTgggaggcaaagagga                                                                                                                                                                                           >  2:109907/1‑225 (MQ=255)                                                     tAAATGGGTCCTGTTGCTCGCGGAATGTTGAGATAAAGCAACGCTGGTTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTagaa                                                                                                                                                                                          <  2:1225528/238‑1 (MQ=255)                                                     aaaaTGGCCCCGGTTGGTCGCGGAAGGTTTAGATAAATCAACGCTGGGTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGGCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTagaa                                                                                                                                                                                          <  2:287989/237‑1 (MQ=255)                                                          ggtgccttttggctcgggAAGTGTTAGAAAAATCAACGCTGGTGGATGGCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCAATGGCGCCCA‑GCCTGGTGGCATCGCCGTGGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTGGTTGTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCAAGAGTAGAAGAGTGc                                                                                                                                                                                    <  1:292079/223‑1 (MQ=255)                                                          ggCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGc                                                                                                                                                                                    <  2:631665/239‑1 (MQ=255)                                                          ggCTCCTGTTGCATGGGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCG‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGc                                                                                                                                                                                    <  1:1205350/239‑1 (MQ=255)                                                                 gTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGAAAACGGGATTTGGTTTTAAACGC‑AAATACCCCTCACGCCTTTTCCCGCAGCCACT‑CGGGCCagaggtgaaaaagggtggcctt                                                                                                                                                                             >  2:1297963/1‑221 (MQ=255)                                                                     ctctCGGAAGGTTTAGATAAATCAACGTGGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAAc                                                                                                                                                                         <  2:925264/239‑1 (MQ=255)                                                                       cccGGAAGGTTTTGATAAATCAACGCTGGGTGGTGTCAAAAAGCA‑GTTGGCCTGGCAAGGTTTTGGCGCACTGGCCCCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTg                                                                                                                                                                       <  1:823652/237‑1 (MQ=255)                                                                        tcGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGc                                                                                                                                                                                                    <  2:475326/209‑1 (MQ=255)                                                                              gtttgagaatactactagctggctaATTTCAAAAAGAG‑GTGTGTCCGGCAAGGTTTGGGCGCTCCGGCCACCA‑GCCTGAGGGCATCGCCGTCGCAAAAACGTGCAGATGTACAGGCACTTT‑TG‑AATGAAGCGGGTGTGTCGGATAACGTGATTTGTTTTGAAGCGC‑AAATCCCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACaaa                                                                                                                                                                 <  1:888109/221‑1 (MQ=255)                                                                               gTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGGagaa                                                                                                                                                                                          >  2:425519/1‑212 (MQ=255)                                                                                   atatgaattcactctggcgtgtGTCAAAAAGCA‑GGTTAACTGGCAAGGGTTTGGCGCAGTGGCACCCA‑GGCTGATGGCCTGGCCGTCGGAAAAACTGGCCGATGTCCAGACACTTT‑TG‑CATGAAGCTGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACGGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGCGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCa                                                                                                                                                           <  1:937338/218‑1 (MQ=255)                                                                                         aTCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGc                                                                                                                                                                                    <  2:393868/208‑1 (MQ=255)                                                                                         aTCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCCCCG‑CACTTCTGGCCAAAGGAAAAGAGTGCTGGGCTTTAAATTAACAAAATGCCATTTACg                                                                                                                                                     >  2:74454/1‑239 (MQ=255)                                                                                         aTCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGGGGTTTGTTTTTAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGGGCCAAAGTAGAAGAGAGCTGGCCTTTAAATGAACAAAAGGGCAtatccg                                                                                                                                                     >  2:1002598/1‑234 (MQ=255)                                                                                         aTCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGAGAACGTGATTTGTTTTGTAGACC‑AAAAACCCCCTGGGCCTTCTGCCGCAG‑CCATGTGTGGCGAAGAAAAAAAGGGCGGGGCTTTACCTGAACaaaaaccacgctaag                                                                                                                                                     >  1:72295/1‑228 (MQ=255)                                                                                          taatcggtggctgAGGCGAAAAAGCA‑GTTGGTCGGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCTGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGG‑AAATGCCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGTCATTTAACTGAACAAAATGCCATGTACGa                                                                                                                                                    <  1:784266/232‑1 (MQ=255)                                                                                          tCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGa                                                                                                                                                    <  2:166164/239‑1 (MQ=255)                                                                                          tCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGGGATTTGTTTTGAAGCGC‑AAAAACCACTGGGGCTTTTTCGCGCAG‑CACTGCGGGCCAGAGTAGAAGAGTGCTGGCATTTAACTTACCAAAATGCCATGTACGa                                                                                                                                                    >  2:206557/1‑239 (MQ=255)                                                                                          aaaccGCTGGCTGTTGCAAAAAAGCA‑GTTGACCGGGCAATGTTTTGGCGCACTGGCACCCA‑GCCTGATGGAAGCGCCGTCGCAAAAACTGGGCTATGTACAGACACCGT‑TG‑CATGAAGCGGGTGTGGCGGATTACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGa                                                                                                                                                    <  1:455810/235‑1 (MQ=255)                                                                                            cacgcgtgctgATGTCAAAAAGCG‑GTTGATTGGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGGCACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGa                                                                                                                                                    <  2:3037/230‑1 (MQ=255)                                                                                              cGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACt                                                                                                                                                                        <  2:389694/215‑1 (MQ=255)                                                                                              cGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGGGATTTTTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGGGCAG‑CACTGCGGGCCAGAGGAGAAGAGTGCTGGCCTTTAACTGAACAAAATGCCATGTACGaaa                                                                                                                                                  >  2:1110905/1‑237 (MQ=255)                                                                                              cGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGAAAACGGGGTTTGTTTTTAAGCGC‑CAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGGGCCCGAATAGAAGAGGGCTGGCATTTAACTGTAACAAATGCCCTGTACGaaa                                                                                                                                                  >  2:1034542/1‑237 (MQ=255)                                                                                               gctggggtatttaaaaaaGCA‑GTTGATCTGGGAAGGTTTTGGCGCACTGGCACCCA‑GGCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCt                                                                                                                                               <  1:1002598/226‑1 (MQ=255)                                                                                                   gctgATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGGTAACGGGATTTTGTTTGTAAGGC‑AAAAACCACTGGCGCCTTCTCCGCCCG‑CACCTCTCGCCGAGGTAGAAGAAGGCCTGCCTTTTACTGAACaaaaatacctggaaggaaacatatt                                                                                                                                           >  1:289817/1‑218 (MQ=255)                                                                                                     ggattgaaaaaaGCT‑GTTGTTTTGGCAAGGGTTGGGCGCCTGGGCCCCCA‑GGCGGATGGCTTCGCCGTCGCAAAACCTGGCCGAAGTACAGGCACTTT‑TG‑CATTAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTCACTGAACAAAATGCCATGTACGAAACCTTTA‑tt                                                                                                                                         <  1:936494/231‑1 (MQ=255)                                                                                                         ttaaaaaaGCA‑TTTGCTCTGGAAATGTTTGGGGGCCCTGGTCCGCACGCGTG‑GGGCATCGCCGTCACAAAACCGGGCCGATTTACAG‑CACCTT‑TGCCATGAGGGGGGTGTGGCGGATATCGTGATTTTTTTTGAAGCGA‑AAATACCCCTGGCGCTTCCTCGCGCAG‑CTCTGCGTGCCAGAGTAGAAGAGTGCTGGCTTTTAACTGAACAAAATGCAATGTCCGAAACCTTTA‑TTCAGTc                                                                                                                                    <  1:1034542/236‑1 (MQ=255)                                                                                                                     gTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTTAAACGC‑AAATACCACTGGGGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGGGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCCTTA‑TTTCGTCATTtcgccccc                                                                                                                         >  2:502467/1‑237 (MQ=255)                                                                                                                     gTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCa                                                                                                                                                                                                  >  2:602411/1‑167 (MQ=255)                                                                                                                     gTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCa                                                                                                                                                                                                  <  1:602411/167‑1 (MQ=255)                                                                                                                          tCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGgcg                                                                                                                                                                                                                            <  2:747140/137‑1 (MQ=255)                                                                                                                             ggacagtttttGGCGCCCTGGCACCCC‑GCCTGATGGCATCGCCGTCGAAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCg                                                                                                                 <  1:212060/232‑1 (MQ=255)                                                                                                                              gCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTTGCGCAG‑CACTGCGTGCCCGAGTAGAAGAGGGCTGGCATTTAACTTAACAAAAAGCCATGTACGAAACCTTTA‑TTCAGGCATTCCGCCCGCTGGTGcc                                                                                                                  >  2:973945/1‑237 (MQ=255)                                                                                                                                aaGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGCTGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACCGGAGTTGTTTTGTAGCGC‑AAATACCCCAGGCGCCTTCTCGCGTAG‑CACGGCGTGCCAGAGGAGAAGAGAGCTGGCATTTTACTGAACAAAATGCAATGTACGAAAACCTTG‑TTCAGTACATCCCCCCCCCGGTGCCCCtt                                                                                                              >  1:670963/1‑239 (MQ=255)                                                                                                                                        ggctgccTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAaga                                                                                                      <  2:873017/233‑1 (MQ=255)                                                                                                                                             aCTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTTAAGCGC‑AAAAACCACCTGCGCCTTTTCGCGCCG‑CACCGCGTGCCAGAGGAGAAGAGGGGTTGCCTTTTACTGGACAAAAAGGCCAGTACGGAAACTTTATTTCAGCATTTCCCCCCCCGGGGGCCCTTTTTAAAAAAgggg                                                                                                  >  1:786193/1‑237 (MQ=255)                                                                                                                                                ggCTCCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACTCTTT‑TG‑CAGGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATCCCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAg                                                                                              <  2:600792/239‑1 (MQ=255)                                                                                                                                                   ccccccgcctttttgcttCGGCGTCGAAAAAACTGGCCGATTTACCGACTCTTT‑TG‑CATGGAGCGGTTTGGGCGGATAACGTGATTTGGTTTGAAGCGC‑AAATACCCCTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGAc                                                                                            <  1:501219/222‑1 (MQ=255)                                                                                                                                                   cccccatgcTGGTGGCATCGCCGGCGAAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCGCTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGGGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGa                                                                                             <  1:921667/230‑1 (MQ=255)                                                                                                                                                   ccccA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGAc                                                                                            <  2:550577/237‑1 (MQ=255)                                                                                                                                                      cA‑GCCTTATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACCCTTT‑TG‑CATGAAGCGGGTGGGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGt                                                                                        <  2:43052/239‑1 (MQ=255)                                                                                                                                                      cA‑GCCTGGGGGCAGCGGCGTTGAAAAAATTGGCCGATGTGCAGACACTTT‑TG‑CATGAAGCGGGTGTGGCTGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAACGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGt                                                                                        <  1:502467/239‑1 (MQ=255)                                                                                                                                                      cA‑GCCTGATGGCCTCGCGGTCGCAAAAGCTGGCCGATTTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGG‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGt                                                                                        <  1:415748/239‑1 (MQ=255)                                                                                                                                                           cTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACCCTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGtt                                                                                       <  2:827987/236‑1 (MQ=255)                                                                                                                                                                          tCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCCTTA‑TTCAGTCATTCCGCCCGCTGGGGCCGCTTTTTAAAAAGGCGGCAGACGAGTTAAACCCGGGGCGGGCAtt                                                                     >  2:926766/1‑239 (MQ=255)                                                                                                                                                                                aatttGGCCGTTGTGCGGGCACTTTGTG‑CTTAAGGCGGGTG‑GGGAGAAAAGGTGATTGGGTTTGAACGGC‑AAAGACCCCTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGACGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACGTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCatat                                                               <  1:1040421/235‑1 (MQ=255)                                                                                                                                                                                aaccttgCCTATGTTCAGACCCTTT‑TG‑CATGAAGCGGGTGGGGCGGTTAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCatat                                                               <  1:206557/233‑1 (MQ=255)                                                                                                                                                                                aaaCTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCatat                                                               <  2:582790/239‑1 (MQ=255)                                                                                                                                                                                aaaCGGGCCGTTGTACTGACACTTT‑TG‑CATGAAGCGGGTGGGGCGGATAACGGGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACCGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCatat                                                               <  1:74454/239‑1 (MQ=255)                                                                                                                                                                                 aattggccgtttttagggcctttt‑tG‑AATGAGGGGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATAtt                                                              <  1:256485/218‑1 (MQ=255)                                                                                                                                                                                          gtGTACCGACTATTT‑GG‑GATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGTGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTa                                                     <  2:800083/238‑1 (MQ=255)                                                                                                                                                                                                   cacTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGAt                                                <  2:72295/235‑1 (MQ=255)                                                                                                                                                                                                      ttt‑tG‑CATGAAGCGGGGTGGGAGTATAACGTGATTGGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTGCATATTCAGCTTTTACTGATCCAtttt                                         <  2:528887/239‑1 (MQ=255)                                                                                                                                                                                                       tt‑tG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTTAAGCGC‑CAATACCACTGGCGCTTTTTCGCGCAG‑CACTGCGTGACAGAGGAGAAACGAGCTGGCATTTAACTGAACCAAATGCCGCGTACGGAAACTTTT‑TTCAGTCATTCTACCCGCTGGGGCCGCTTTTTAAAAAGGCGGTAGACGAGTTAACCCCGGAGCGGGGACTTTTTATTTTCCTTTTAccaggaccttttt                                        >  1:754120/1‑227 (MQ=255)                                                                                                                                                                                                       tt‑tG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTTAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAAACTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCCTTTAAAAGAGGCGGCAGACGAGTTAACCCCCGGGGGGGCCTTTCATATTCACCTTTTACTGGTCCAttttt                                        >  2:1269662/1‑239 (MQ=255)                                                                                                                                                                                                       tt‑tG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCCGAGCGGGCCTTTCATATTCAGCTTTTACTGATCCAttttt                                        >  2:339880/1‑239 (MQ=255)                                                                                                                                                                                                        t‑tG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTa                                       <  2:801901/239‑1 (MQ=255)                                                                                                                                                                                                                  aGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGGGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGGCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCCTTTCCTTTTCAGCTTTTACTTATCCATTTTTAtttccggg                               >  2:742706/1‑232 (MQ=255)                                                                                                                                                                                                                     gggTGTGGGGGTTAACGTGATTTGTTTTGAAGCGC‑AAATACCCCTGGCGCTTTCCGCCGCAG‑CACTGCGTGCCAGAGTAGAAGTGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTgtcg                            <  1:1011781/239‑1 (MQ=255)                                                                                                                                                                                                                     gggTGTGGCGTGTAACGTGATTTGTTTTGAAGCGC‑AAATCCCCCTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTgtc                             <  2:389125/238‑1 (MQ=255)                                                                                                                                                                                                                     gggTGTGGCGGATAACGGGATTTGGTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTgtcg                            <  2:1320868/239‑1 (MQ=255)                                                                                                                                                                                                                      ggTGTGGCTGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTgtcg                            <  2:326477/238‑1 (MQ=255)                                                                                                                                                                                                                        tgtgGCGGATAACGGGATTTGTTTTTAAGCGC‑AAATGCCACTGGCGGTTTCTCGCGCAG‑CAGTGCGTGCCTGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAGCCTTTA‑TTCAGTCATGCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTgtcgtc                          <  2:424427/238‑1 (MQ=255)                                                                                                                                                                                                                                     gTGGTTTGTTTTGAAGCGC‑AAATACCCCTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTGTCGTCCTTAAAGACCCAtt            <  2:670963/239‑1 (MQ=255)                                                                                                                                                                                                                                             ttttGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTGTCGTCCTTAAAGACCCATTGTTGCCgg    <  2:289817/239‑1 (MQ=255)                                                                                                                                                                                                                                               ttGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTGTCGTCCTTAAAGACCCATTTTTGCCggag  >  2:704505/1‑239 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                CGCGACGGGCAGAAAGTAAAATTTATCGCGCAGAGCAACCTGCATGGGATGGTAAATGGCTCCTGTTGCTCTCGGAAGGTTTAGATAAATCAACGCTGGCTGATGTCAAAAAGCA‑GTTGATCTGGCAAGGTTTTGGCGCACTGGCACCCA‑GCCTGATGGCATCGCCGTCGCAAAAACTGGCCGATGTACAGACACTTT‑TG‑CATGAAGCGGGTGTGGCGGATAACGTGATTTGTTTTGAAGCGC‑AAATACCACTGGCGCTTTCTCGCGCAG‑CACTGCGTGCCAGAGTAGAAGAGTGCTGGCATTTAACTGAACAAAATGCCATGTACGAAACCTTTA‑TTCAGTCATTCCGCCCGCTGGTGCCGCTTTTAAAAGAGGCGGCAGACGAGTTAACCCCGGAGCGGGCATTTCATATTCAGCTTTTACTGATCCATTTTTATCGCCGTGTCGTCCTTAAAGACCCATTGTTGCCGGAG  >  NC_000913/1463810‑1464282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑