BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,054,696	C→T	5.9%	L352L (CTG→TTG)	shiA →	shikimate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,054,696	0	C	T	5.9%	369.6 / 2.0	122	L352L (CTG→TTG)	shiA	shikimate transporter
Reads supporting (aligned to +/- strand): ref base C (66/47); new base T (7/2); total (73/49)
Fisher's exact test for biased strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

TAATTATTCAACCCAGAATATGGGGCTACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACTCATCACTTACTTTGCCGGGAACTGGCATAGCGTCGCCATTTATTTGCTGGCTGGATGCCTGATTTCCGCAATGACCGCTTTGTT  >  NC_000913/2054464‑2054934

tAATTATTCAACCCAGAATATGGGGCTACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGTTAGTTTTCTTCT‑CCATAATGTTGGCaa                                                                                                                                                                                                                                                >  2:579295/1‑239 (MQ=255)

     taaaaggcaggAATATGGGGTTACCCCGCGTACTTTTCCTTTGTATTGGTTGGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCGTGTTTTGCCTGGCTGTCCGATCGTTTTGTTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACAtt                                                                                                                                                                                                                                           <  1:981434/229‑1 (MQ=255)

        cAACCCGAAATATGGTGTTACCGCGGGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTgcg                                                                                                                                                                                                                                        <  1:119395/239‑1 (MQ=255)

         aaCCCAGAATATGGGGCTACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTgcgc                                                                                                                                                                                                                                       >  2:889027/1‑239 (MQ=255)

         aaCCCAGAATATGGGGCTACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGACTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCATAGGGTTTATATCACCGGTACGTTAATCGGAAGGTTGCACGCATTTGATTTTTTTATTGCGCTTGAAGAACAAACTATTTCTTGAAAATTTTTCTTTT‑CAATAATCTTGGCAAACATTggca                                                                                                                                                                                                                                       >  1:394142/1‑236 (MQ=255)

           cccAGAATATGGGGCTACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGACCGTTGAGCCCATTTCCTTTCTTTTTGGCCCTTGAAGGACAATCTATTTTCTGGTTGGTTTTCTTTT‑CACTAATTTTGGCAAACATTgggctg                                                                                                                                                                                                                                     >  1:1000843/1‑237 (MQ=255)

            ccAGAATTTGGGGCTACCGGCGGAACCTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCa                                                                                                                                                                                                                                      <  2:374711/237‑1 (MQ=255)

            ccAGAATATGGGGCTACCGCGGGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATg                                                                                                                                                                                                                                    <  2:1077754/239‑1 (MQ=255)

              tgatattGGGGCTTCCTGGCGAATTTTTCCTTAATATTGGTTTGCTGGTAGTTGGATTAAGCTGTCTGTCAATTGCCTGTTTTTCCTGGCTTGCCGATCGTTTTGGTCGCCGTGGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGAc                                                                                                                                                                                                                                  <  1:100320/233‑1 (MQ=255)

                aaTATGGGGCTACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑t                                                                                                                                                                                                                             >  2:111449/1‑239 (MQ=255)

                 ttttgggggttccggggtaaCTTTTCCTTTATTTTGGTTTGGTGGTGGGGGGATTAAGCTGCCGGACAATTCCCTGTTTTGCCGGGCTTGCCGATCGGTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑t                                                                                                                                                                                                                             <  1:376829/220‑1 (MQ=255)

                     ggggCTACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCTGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTTAAGCACAATCTATTTTCTGGATCGTTTTTTTCT‑CCATAATGCTGGCAAAAACTGCGCAGGAAA‑‑‑tggtgg                                                                                                                                                                                                                        >  1:346058/1‑239 (MQ=255)

                     ggggCTACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCTTAATGCTGGCAAACATTGCGCATGCCA‑‑‑tggtgg                                                                                                                                                                                                                        >  2:1050177/1‑239 (MQ=255)

                     ggggCTACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTACCTGCTTTGCCCGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATACAACAGGTACGTTAATCGGAACGTTGACCCAATTTCCTTCCTTTATGGCGCTGCAAGCACAATCTTTTTGTAGGATAGTTTT‑TTCTCCCATAATATTGGCaaaactgtgcattgaaaggtggg                                                                                                                                                                                                                           >  1:374711/1‑219 (MQ=255)

                     gcggttACCGCGCGAACTTTTCCTTCATATTGGCTTGCTGGTAGGTGGATTACGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGCCTTGCTCGCCGTCGGGTTTCTATCACCGGTACGTTAATCGGAACGGTGTGCGCATTTCCTTTCTTTATGGGGCTTGTAGCACAATCTCTTTTCTGGATAGTTTTCTTCT‑CCTTTATGTTGGCAAACAGTGCCCACGActgagtgg                                                                                                                                                                                                                           >  1:431164/6‑232 (MQ=255)

                          tACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGTTGGCAAACATTGCGCATGACA‑‑‑TGGGGgtgtgt                                                                                                                                                                                                                   >  2:692195/1‑239 (MQ=255)

                          tACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTgcg                                                                                                                                                                                                                                        >  1:1241056/1‑221 (MQ=255)

                          tACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTgcg                                                                                                                                                                                                                                        <  2:1241056/221‑1 (MQ=255)

                          tACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGAAA‑‑‑tctgtctcttt                                                                                                                                                                                                                   >  2:981434/1‑229 (MQ=255)

                          tACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGAAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATTCTGGAAAACATTGCGAAAGACA‑‑‑tggtggggggg                                                                                                                                                                                                                   >  1:490421/1‑234 (MQ=255)

                                                       ttGCTGGTAGGTTGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGtt                                                                                                                                                                                    <  2:288053/239‑1 (MQ=255)

                                                        tGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGGGTGTG‑TGCAAAAAC‑CGGTGTTTACCGAAATGttt                                                                                                                                                                                   >  1:1240593/1‑239 (MQ=255)

                                                         gCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTg                                                                                                                                                                                  >  2:202673/1‑239 (MQ=255)

                                                          cTGGTCGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTCTTCGACACGTTGAGCGCAGTTCCTTTCTTTATGTCGCTTGAAGCCCACTCTTTTTTCTCGATAGTTTTCTTCT‑CCATATTGCTGGCACACCTTGCGAATGACC‑‑‑TGGGGTTGTGTT‑TACAACAAC‑CGCTGTTTACCGAAATGTTTgg                                                                                                                                                                                 >  1:319936/1‑239 (MQ=255)

                                                          cTGGTAGGTGGATTCAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGGACGTTAATCGGCACGTTGCGCGCCTTTCCTTTCTTTATGGCCCTTGAGTCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCTCAATGTTGGCAAACATTGCACATGACC‑‑‑TGTGGGGCTGTG‑TGC‑CCAACACGAGGTTTCCGGAAATGTTCgg                                                                                                                                                                                 >  1:265836/1‑239 (MQ=255)

                                                          cTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGCGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGCTAGTTTTCTTTT‑CCCTAATTCTGGCAAACCTTGAGCATGCCA‑‑‑TGGTGGGGTGTG‑TGCAACACC‑CGCTGTTTACCGAAATGTTTgg                                                                                                                                                                                 >  1:20730/1‑239 (MQ=255)

                                                             gttggtggtTTAAGCTGCCTGGCAAGTCCTTTTTTTGCCTGGCTTGCCGATCGTTTTGGGCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGc                                                                                                                                                                              <  1:290970/236‑1 (MQ=255)

                                                             gtgggTGGATTAAGCTTCCTGGCAATTCCCTGTTTTGCCTGGCTTGCCGGTCGTTTTGGGCGGCGTAGGGTTTATATCCCAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGc                                                                                                                                                                              <  1:1050177/236‑1 (MQ=255)

                                                                 gttgttttatcttcctgctaaTTCCCTTTTTTGCCTTGGTTTCCGTTCGGTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGt                                                                                                                                                                          <  1:1026884/220‑1 (MQ=255)

                                                                  tGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGt                                                                                                                                                                          <  2:1267778/238‑1 (MQ=255)

                                                                    gATTTAGGTTCCTGTGAATTCCCTGTTTGTCCTGGCTTGCCGATCGTTTTGGGCGCCCTAGGGTTTATATCACAGGTACGTTAAGCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGGTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑GGATGTTTACCGAAATGTTTGGTGCCAGTTAt                                                                                                                                                                       <  1:884225/239‑1 (MQ=255)

                                                                    gATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCTATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTAt                                                                                                                                                                       <  2:1305476/239‑1 (MQ=255)

                                                                    gATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCCCAATCTATTTTCTGGATAGTTTTTTTCT‑CCATAATGCTGGCAAACATTTCGTATTCAA‑‑‑TGGTGG‑GTGTGTTGCAACAAC‑CCATGTTTCACGAAATTTTTTTTGCCAGTTa                                                                                                                                                                        >  1:1267778/1‑238 (MQ=255)

                                                                    gATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGCTAGTTTTCTTCT‑CCATAATGCTGGCAACAAATTCGCATTACC‑‑‑AGTTGGTTTGGG‑TGCCACAAC‑CGACTTTTACCGAAAAGTTTGGTGACGGtttt                                                                                                                                                                       >  1:738639/1‑237 (MQ=255)

                                                                     agtAAGGTGCGTGGCAATTCCCTGGTTTGCCTGGCTTGCCGCTAGTTTTGGTCCGCGGAGGGTTTATTTCGCAGGAACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTAt                                                                                                                                                                       <  2:1263365/236‑1 (MQ=255)

                                                                        aaGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTCGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCAAAATCTATTTTCTGGATAGTTTTCTTTT‑CCATATTGCAGGCAAACATTTCGCTTTACA‑‑‑TGGTGGTGGTTG‑TGCAACACC‑CAGTCTTTACCGAAATGGTTGGTGCCGGTAAtgcgt                                                                                                                                                                   >  1:1022270/1‑235 (MQ=255)

                                                                              ccTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGCTGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTg                                                                                                                                                             >  2:600104/1‑239 (MQ=255)

                                                                              ccTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTg                                                                                                                                                             >  2:268456/1‑239 (MQ=255)

                                                                                        cccTTTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGt                                                                                                                                                   <  2:540149/239‑1 (MQ=255)

                                                                                        cccTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGAATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGt                                                                                                                                                   <  2:394142/239‑1 (MQ=255)

                                                                                        cccTGTTTTCCCTGGGTTGCCCATCTTTTTTGTTGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTGTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGt                                                                                                                                                   <  1:18368/239‑1 (MQ=255)

                                                                                        cccTGGTTGGCCTGGGTTGCCGATCGTTTTTGGCGCCGTAGGGGTTATATCACAGGTACGTTAAACGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGt                                                                                                                                                   <  2:43131/239‑1 (MQ=255)

                                                                                           tGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGATGGAGTCgg                                                                                                                                                >  2:699373/1‑239 (MQ=255)

                                                                                             ttttgccttgttttcctttttttttTGTCGCCGTGGGTTTTATATTACAGGTACGTTAACCGGAACGTTGGGCGCATTTCCTTTCTGTATGGGGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGTTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGtt                                                                                                                                              <  1:692195/218‑1 (MQ=255)

                                                                                             ttttGTCTTGCTTGTCGTTCGTTTTGGTCGCCTTAGGGTTTATATCACAGGTGCGTTAATCCGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGAATAGTTTTCTTCT‑CCATAATGCTGGCAACCATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGtt                                                                                                                                              <  1:831733/239‑1 (MQ=255)

                                                                                             ttttGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGc                                                                                                                                                                                                              >  2:173835/1‑176 (MQ=255)

                                                                                             ttttGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGc                                                                                                                                                                                                              <  1:173835/176‑1 (MQ=255)

                                                                                                tggcTGGCTTGCCGATCGATTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGCGCGCATTTCCTTTCTTTATGGCGCTCGAAGCACAATCTATTTTCTTGGTCGTTTTCTTCT‑GCCGAATGCTGGCAAACATTGCGCCTGAAC‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGCGGTTTACCGAAAGCTTTGGTGCCAGTTTGCGCTACATGGGCGCGGGGTTCGGTAAtg                                                                                                                                           >  1:743912/4‑238 (MQ=255)

                                                                                                    tGGGTTGCCGATCGGTTTGGTCGACGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCTCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATTGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGt                                                                                                                                       <  2:192605/239‑1 (MQ=255)

                                                                                                       cTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGGGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGGGACGCTGAATACGGTTATCAGGTTg                                                                                                                                     >  2:1014612/1‑238 (MQ=255)

                                                                                                        ttGCCGTTTGTTTTGGTTGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCTGATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGc                                                                                                                                    <  1:1125964/238‑1 (MQ=255)

                                                                                                           gcgttgtttttGTGCGCCGTTGGGTTTATTACACAGGTTGGTTAAGCGGAACGTTGAGCGAATTTCCTTTCTTTATGGCGTTTGAAGCACAATCTATTTTCTGGATAGTTTTCTGCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAg‑t                                                                                                                               <  1:410267/232‑1 (MQ=255)

                                                                                                                       ggcGCCGTTGGGTTTATATCACAGGTACGTTAATCGGACCGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGt                                                                                                                   <  1:298356/237‑1 (MQ=255)

                                                                                                                       gTCGCCGTATGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGGTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGt                                                                                                                   <  1:859027/239‑1 (MQ=255)

                                                                                                                            cGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGt                                                                                                                          <  2:764029/227‑1 (MQ=255)

                                                                                                                                     tataTCACAGGTACGATAATCGGAACGTTGAGCCCTTTTCCTTTCTTTCTGGCGCTTGAAGCACAATCTATTTTTTGGTTTGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TTCAACAAC‑CGATTTTTACCGAAACGTTTGGTGCCGGTTATCTCTATAATCGGGCTGAAGTCGGGTATCACGTTTCCAG‑TTTgggttggggggtatttcaacatct                                                                                                     >  1:124579/1‑217 (MQ=255)

                                                                                                                                      atatCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCTTAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTTTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGGGGTTGGCGGGGGATTTAAACCtttt                                                                                                    >  1:127061/1‑239 (MQ=255)

                                                                                                                                      atatCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTT‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCGGTTATCGCTATAGTGGCGCTGGCGCCGGTTATCAGGTTGCCAG‑TGGGGTTTGGGGTGGAtctggtcttttt                                                                                                    >  1:764029/1‑228 (MQ=255)

                                                                                                                                      atatCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TTTGGTTGGCGGTGGATTTAAACCtttt                                                                                                    >  2:202452/1‑239 (MQ=255)

                                                                                                                                      atatCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCtttt                                                                                                    >  2:485942/1‑239 (MQ=255)

                                                                                                                                      atatCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATACGGTTGCCAG‑TGGGGTTGGCGGGGGATTTACACCtttt                                                                                                    >  2:699709/1‑239 (MQ=255)

                                                                                                                                        atCCCAGGTTCGTTAATCGGAACTTTGACCGCATTTCCTTTCTTTATGGCGGTTGAAGCACAATCAATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGGGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTAt                                                                                                  <  1:889027/239‑1 (MQ=255)

                                                                                                                                           acaGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTg                                                                                                >  2:1167282/1‑238 (MQ=255)

                                                                                                                                           acaGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATAGCTATAGTGGCGGTGGAGTCGGTTATCCGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTg                                                                                                >  2:365555/1‑238 (MQ=255)

                                                                                                                                            caGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACCTTGCGCATGACA‑‑‑TGGCGGCGTGTG‑TGCAACAAC‑CGATGTTTACCGCAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGCGTCGGTTATCAGCTGGGCAG‑GGTGGTTATAGGGGGATTTACACCATTTaattcc                                                                                              >  1:48847/1‑234 (MQ=255)

                                                                                                                                            caGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGGCGGTTATCAGGTTGCCAG‑TGTGGTTGGAGGGGGTTTTACCCCtttttatacc                                                                                              >  1:458435/1‑233 (MQ=255)

                                                                                                                                            caGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑GGCAACAAC‑CGATGTTTACCGAAATGTTTGTGGCAAGTTATCGCTAAAGTGGGGCTGGAGTCGGTTATCTGGTTGCCAGTTGGGGTTGG‑GGGGGGTTTAAACCTTTTTTTGcc                                                                                              >  1:1295374/1‑239 (MQ=255)

                                                                                                                                             aGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCa                                                                                                                                                                                                                                      <  2:933542/108‑1 (MQ=255)

                                                                                                                                             aGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCa                                                                                                                                                                                                                                      >  1:933542/1‑108 (MQ=255)

                                                                                                                                             aGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACATGGTGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGTGGGATTTACACCTTTTAtt                                                                                                 >  2:461771/1‑238 (MQ=255)

                                                                                                                                             aGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATGGCTTTAGTGGCGCTGGAGTCGGTTACTAGGTTGCCCG‑GGTGGTTGGGGGGGGGTTTACACCTTTTATTGcgg                                                                                             >  1:543849/1‑237 (MQ=255)

                                                                                                                                             aGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGGGGATTTACACCTTTTATTGcc                                                                                              >  2:1224366/1‑238 (MQ=255)

                                                                                                                                             aGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCCATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGcc                                                                                              >  2:304571/1‑238 (MQ=255)

                                                                                                                                             aGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGAAA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTAAACCtttatttccc                                                                                              >  2:325023/1‑231 (MQ=255)

                                                                                                                                               gTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACACC‑CGATGTTTACCGAAATTTTTGGGGCCAGTTATCGCTAAAGTGGCGCTGGAGTCGGTTATCAGGTTGCCCG‑TTGGGTTTGCGGTGGGTTTAAACCTTTTTTTGCCGcg                                                                                           >  1:1147029/1‑238 (MQ=255)

                                                                                                                                                ttcGTTAATCGGAACGTTGAGCGGATTTCCTTTCTTTATGGGGCTTGAAGCACAATCTATTTTCGGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATGGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCt                                                                                           <  1:202452/236‑1 (MQ=255)

                                                                                                                                                tACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCg                                                                                             <  2:1097366/236‑1 (MQ=255)

                                                                                                                                                gccGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCTCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACAGTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCg                                                                                             <  2:1132402/234‑1 (MQ=255)

                                                                                                                                                   gTTAATCGGAACGTTGAGCGCATTTCCTTTAGTTATGGCGCTTGAATCACAATCTATTTTCGGGATAGTTTTCTTCT‑CCATAAGGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACTCCTTTTATTGCCGCTGCAc                                                                                       <  2:241741/239‑1 (MQ=255)

                                                                                                                                                       aTCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACtca                                                                                    >  2:692533/1‑238 (MQ=255)

                                                                                                                                                            aaCGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATATGGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACTCATCactt                                                                              >  2:344660/1‑239 (MQ=255)

                                                                                                                                                               gTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACTCATCacttact                                                                           <  2:20730/239‑1 (MQ=255)

                                                                                                                                                                     cgcTTTTCCTTTTTTTTTTGCTTTTGAAGCACAATTTTTTTTCGGGTTAGTTTTTTTTC‑CCAAAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACTCATCACTTACTTTGCCg                                                                     <  1:591399/239‑1 (MQ=255)

                                                                                                                                                                        aTTTCCTTTCATTGCTGGGCATGAAGCACAATCTATTTTCTGGAATGTTTTCTTCC‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGGTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACTCCTTTTATTGCCGCTGCACTCATCACTTACTTTGCCggg                                                                   <  2:455143/238‑1 (MQ=255)

                                                                                                                                                                          ttCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGGGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCGGCACTCATACCTTACTTTACCGGGaag                                                                >  1:1258315/1‑238 (MQ=255)

                                                                                                                                                                                     ttggcgtcttaagcaaaagtgtgtttCTGGTAAGTTTTCTTCT‑CCAAAAGTTTGGTCAACTATGCGCTTTGCT‑‑‑TTGTGGTGTGTT‑TGAACCAAC‑CGTAGTTTAGCGAAATTTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACTCATCACTTACTTTGCCGGTAACTGGCATAGCGt                                                     <  1:261426/216‑1 (MQ=255)

                                                                                                                                                                                         cgcTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACTCATCACTTACTTTGCCGGGAACTGGCATAGCGTCGc                                                  <  2:1000843/238‑1 (MQ=255)

                                                                                                                                                                                           cTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTTGTGCCAGTTATCGCTATAGTGACGCTGGATTCGGTTATCAGGATGCCAG‑GTGGGTTGGGGGTGGGTTTACACCTTTTTTTGCACCTGAACCCATCCCTTTATTTGCCGGGAAATGGCACAGCGGCGccct                                               >  1:1164894/1‑237 (MQ=255)

                                                                                                                                                                                           cTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACCCATCACTTACTTTGCCGGGAACTGGCATAGCGTCGCCat                                               >  2:604309/1‑239 (MQ=255)

                                                                                                                                                                                            ttGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACTCATCACTTACTTTGCCGGGAACTGGCATAGCGTGGCCatt                                              >  2:135960/1‑239 (MQ=255)

                                                                                                                                                                                                                   aTAGTTTTCTTCT‑CCATAATGCTGGCAAACATTACGCATGACA‑‑‑TGGTGGTGTGTT‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCCGTTATCGCTATAGTGGCGCTGAATTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGTGGGATTTACACCTTTTGATCTCGCTGCACTCATGACTTACTTTCCGGGGAAATGGCATATGGTCGCCATTTATTTGTTGGCTGGATTccgggt                       >  1:41607/1‑235 (MQ=255)

                                                                                                                                                                                                                     aGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACG‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACTCATCACTTACTTTGCCGGGAACTGGCATAGCGTCGCCATTTATTTGCTGGCTGGATGCCTGAtt                      >  2:1135310/1‑238 (MQ=255)

                                                                                                                                                                                                                       gtttCTTCT‑CCATAATGCTGGCAAACATGGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGAGGATTTACACCTTTTATTGCCGCTGCACTCATCACTTACTTTTCCGGGAAAGGGCCAAGCGGTGCCATTTTATTGTTGGCTGGTAGCCTGATTTcg                   >  1:978857/2‑238 (MQ=255)

                                                                                                                                                                                                                                  cATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACTCATCACTTACTTTGCCGGGAACTGGTATAGCGTCGCCCTTTATTTGCTGGCTGGATGCCTGATTTCCGCAATGAcc          >  2:607801/1‑238 (MQ=255)

                                                                                                                                                                                                                                    tAATGCTGGCAAACATTGCGCATTACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACTCATCACTTACTTTGCCGGGAACTGGCATAGCGTCGCCATTTATTTGCTGGCTGGATGCCTGATTTCCGCAATGACCGCt       <  2:543849/239‑1 (MQ=255)

                                                                                                                                                                                                                                    tAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACTCATCACTTACTTTGCCGGGAACTGGCATAGCGTCGCCATTTATTTGCTGGCTGGATGCCTGATTTCCGCAATGACCGCt       <  2:458435/239‑1 (MQ=255)

                                                                                                                                                                                                                                       tGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGt                                                                                                                                                   >  1:154955/1‑97 (MQ=255)

                                                                                                                                                                                                                                       tGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGt                                                                                                                                                   <  2:154955/97‑1 (MQ=255)

                                                                                                                                                                                                                                        ccTGGCAAACATTGCGACTGACA‑‑‑TGGGGGGGGTTG‑TGCAACAAC‑CGATGTTTACCGAAATGGTTGGTGCCAGTTATCGCTATAGTGGCGCTTGAGTCGGTTAGCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGTCGCTGCACTCATCACTTACTTTGCCGGGAACTGGCATAGCGTCGCCATTTATTTGCTGGCTGGATGCCTGATTTCCGCAATGACCGCTttgt   <  2:215799/238‑1 (MQ=255)

                                                                                                                                                                                                                                         cTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTCCACCTTTTATTGCCGCTGCACTCATCACTTACTTTGCCGGGAACTGGCATAGCGCCGCCATTTATTTGATGGCTGGATGCCTGCTTTCCCCAATGACCCCtttttt  >  2:1280162/1‑239 (MQ=255)

TAATTATTCAACCCAGAATATGGGGCTACCGCGCGAACTTTTCCTTAATATTGGTTTGCTGGTAGGTGGATTAAGCTGCCTGACAATTCCCTGTTTTGCCTGGCTTGCCGATCGTTTTGGTCGCCGTAGGGTTTATATCACAGGTACGTTAATCGGAACGTTGAGCGCATTTCCTTTCTTTATGGCGCTTGAAGCACAATCTATTTTCTGGATAGTTTTCTTCT‑CCATAATGCTGGCAAACATTGCGCATGACA‑‑‑TGGTGGTGTGTG‑TGCAACAAC‑CGATGTTTACCGAAATGTTTGGTGCCAGTTATCGCTATAGTGGCGCTGGAGTCGGTTATCAGGTTGCCAG‑TGTGGTTGGCGGTGGATTTACACCTTTTATTGCCGCTGCACTCATCACTTACTTTGCCGGGAACTGGCATAGCGTCGCCATTTATTTGCTGGCTGGATGCCTGATTTCCGCAATGACCGCTTTGTT  >  NC_000913/2054464‑2054934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`