BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,064,843	A→C	12.6%	V127G (GTG→GGG)	cobS ←	cobalamin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,064,843	0	A	C	12.6%	237.2 / 8.6	109	V127G (GTG→GGG)	cobS	cobalamin synthase
Reads supporting (aligned to +/- strand): ref base A (54/36); new base C (4/14); total (58/51)
Fisher's exact test for biased strand distribution p-value = 4.20e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TAGCAGCCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACAAGTACGCTAAACAGTGCCGCCAGTGGTGCGCCACACCATGCCTGCAGCACCATGAAGACC  >  NC_000913/2064610‑2065073

tAGCAGCCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATCCGGCAGTCCCGCGGCTGACCGCCCATGCTGCCCCCAGCGATGCAAGGATCGATTCGCCACGCAACGCCAACTCCC‑TTACAACCAGa                                                                                                                                                                                                                                       >  2:660950/1‑239 (MQ=255)

     gcCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCCATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGAATCGATTCGCCCCGCAAGGCCAACTCCC‑TTAAAACCAGAATcct                                                                                                                                                                                                                                  >  2:830862/1‑237 (MQ=255)

     gcCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTACCACCAGAATCtt                                                                                                                                                                                                                                  >  2:459427/1‑239 (MQ=255)

        ccacCATGCAGTCCCGGACATAATACGGCAACAAAAAGACGGGCGAAACCGAGTTGGAAGCAGGTTTGCGCCCCACAAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCGTCTT‑CCCGCGCATAAC‑GATGACGATACAGCAATAATGCGGCAGTCCCGCGGCTGAACGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGc                                                                                                                                                                                                                               <  1:896508/238‑1 (MQ=255)

        caacCATGCATCACCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACCCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGc                                                                                                                                                                                                                               <  2:533426/237‑1 (MQ=255)

           ccATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑g                                                                                                                                                                                                                           <  2:368063/239‑1 (MQ=255)

             aTGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCATTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAAAGCCAACTCGC‑TTACACCCAGAATCTTTGCCC‑Gt                                                                                                                                                                                                                          >  2:456327/1‑238 (MQ=255)

              tGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAACGCCAACTCGC‑CTAACACCAGAATCTTTGCCA‑GTa                                                                                                                                                                                                                         >  2:1216295/1‑238 (MQ=255)

              tGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGACCTTCTT‑CCCGCGCATAAC‑GACGACGATACATCACTAATGCGGCAGTCCCGCGGCCGACCGCACATGCTCCCGCCAGCGACGCAAGGATCGATCCGCCACGCAGAGCCACCCCGC‑TCAACACCACAACCTTTGGCA‑CTa                                                                                                                                                                                                                         >  1:1242422/1‑238 (MQ=255)

                  tgcccGGAAATAATTGGGCAGCAAAAATAGGGGACAAGCCGAGTGTGGCGCTTGTTTGGCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAGGCCCTTCTT‑CCCGGGCATAAC‑GATGACGATACATCAATTATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACcac‑a                                                                                                                                                                                                                   <  1:1091683/237‑1 (MQ=255)

                  tACCCGGCAATAATACGGCAGAAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACcac‑a                                                                                                                                                                                                                   <  2:1104054/239‑1 (MQ=255)

                   aCCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCCGAATCTTTTCCA‑GTACCAC‑aa                                                                                                                                                                                                                  >  2:455402/1‑239 (MQ=255)

                    cccGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCCATGAACACA‑TTGCCAAGCCATTCTT‑CCCGAGCATAAC‑GATGACGATACATCAATAATGCGGCCGTCCCGCGACTGACCACACATGCTGCCGCCAGCGATGCAAGGATCGGTTAGCGCCGCAGAGACACCCCCA‑CTAAAACCAGAATCTTTGcacgcaacaacaa                                                                                                                                                                                                                   >  1:528982/1‑227 (MQ=255)

                    cccGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACCTGCTTCCGCCCGCGATGCAAGGATGGATTCGCCACCCAAAGCCCAACCCC‑TTAACCCCAGAATCCTTCCAC‑GTCCCAC‑aaa                                                                                                                                                                                                                 >  1:847453/1‑239 (MQ=255)

                    cccGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCACCGATGCAAGGATCGATTCGCCCCGCAGAGCCAACTCGC‑TTAACACCAGAACCTTGTCCA‑GAACCCC‑aaa                                                                                                                                                                                                                 >  2:472900/1‑239 (MQ=255)

                     ccGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATTCTGCCCCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCCC‑aaaa                                                                                                                                                                                                                >  2:564549/1‑239 (MQ=255)

                       gggaataatACGGGACGAAAAACTGCGGCTAAGCCGAATGTGAAGCAGGTTTGTCGCCCAACAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAAt                                                                                                                                                                                                              <  2:535994/236‑1 (MQ=255)

                        gCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCCC‑TTAACACCAGAATCTTTGCCA‑GTACCCC‑AAAAATc                                                                                                                                                                                                             >  2:458500/1‑239 (MQ=255)

                        gCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCAAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAAGGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑acaaaca                                                                                                                                                                                                             >  2:46188/1‑237 (MQ=255)

                        gCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGAGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTTCCCCGCGCATAAC‑GATGACGAAACATCAATAATGCGGCAGTCCCGCGGCTGCCCGACCAAGCTGCCGCCAGCGATGCACGGATCGATTCGCCACGCACGGCCAACTCGC‑TTAACACCAGAACCTTTGcacgtccccccaaaaa                                                                                                                                                                                                                >  1:190173/1‑224 (MQ=255)

                        gCAATAATACGGCAGCAAAAATAGCGGCTAAACCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCAAAAC‑GATGACGATACCTCAATAATGCGGCACTCCCCCGGCTGCCCCCCCATGCTGCCGCCAACCATGACCGGATCGATTCCCCCCCCACAACCAACTCCC‑TTACCCCCCAGATCTTTTCCA‑GGAACCC‑aaacaac                                                                                                                                                                                                             >  1:1086936/1‑235 (MQ=255)

                         cAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCTGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACCCGG‑TTAACACCAGCATCATTGCCA‑GTACCCC‑AAAAATCa                                                                                                                                                                                                            >  2:45117/1‑239 (MQ=255)

                               atttttttaagaggaaGCGGCTAAGCCGAGTGTGACGGAGTTTTTGCCCCCCACAATTT‑TGCCAATGAACCCA‑TTGCCAAGCCCTTCCT‑CCCGCGCAAAAC‑GATGACGATACATCAATAATGCGGCGGTCCCGCGGCTGCCCGACCATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCa                                                                                                                                                                                                      <  1:822103/224‑1 (MQ=255)

                                 cGGCACCAAAATTTGCGGCTAACGTGTATGGGAGGCTGGTTTTTACCCCAACAATTTGTGCAAACAACCAC‑‑TTGCAAGGCCCATCTT‑CGCGCGTATAAC‑GATGACGATACATCAATAATGCGGCAGTCCAGCGGCTGACCGCACATGCTGCCGTCAGCGATGCACGGACCTATTCGCCTCGCAGAGCCACCTCGC‑TTAACACCACTCTCTTTGTCA‑TTGCCCC‑ACAAACCAATGcctgc                                                                                                                                                                                                    <  1:503364/239‑4 (MQ=255)

                                      gcaAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTTCCCACAGCGATGCAAGGATCGGTTCGCCACGCCAGGCCAACCCCA‑TTAAACCCCGAAACTTTGCCC‑GAACACC‑AAAAATCaaaaccaaaaccac                                                                                                                                                                                               >  1:637809/1‑227 (MQ=255)

                                       caAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGGCGCCAGCGATGCGAGGAGCGATTCGCCACGCAGAGCAAACCCCC‑TTCACACCAAAAAACTTGCCC‑GCACCAA‑CAAAATAAAAGCCaaaacaccg                                                                                                                                                                                              >  1:905330/1‑231 (MQ=255)

                                                 gcgtaagcctgatttgccGCCGGTTTTCCCCCCCTAAATTT‑CTCCATGGAACACT‑TTACCAGGCCCCTCCT‑CCCGCGC‑TAACTGATTATCATCCAACAATAATGCGGCAGTCCCGCGGCTGCCCGCACATGCTGCCGCCAGCGCTGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACCCCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTa                                                                                                                                                                                   <  1:46188/222‑1 (MQ=255)

                                                  gCTAAGCCGAGGGTGACGCAGGTTTGTCCCCCATCAATTT‑TTCCAATGAACACA‑TTGCCAAGCCCTTCTC‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTa                                                                                                                                                                                   <  2:799365/238‑1 (MQ=255)

                                                           aGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTg                                                                                                                                                                                           >  2:822103/1‑221 (MQ=255)

                                                            gtgtGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑TTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACAGCTTTc                                                                                                                                                                         >  2:675459/1‑238 (MQ=255)

                                                                 aCGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCa                                                                                                                                                                    <  2:689007/238‑1 (MQ=255)

                                                                  cGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATACCCA‑ACCCCCATGGGGGCCAAAAAGGCCAacaccaata                                                                                                                                                                  >  1:124454/1‑234 (MQ=255)

                                                                     aGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCCCCCATGAt                                                                                                                                                                >  2:1030312/1‑238 (MQ=255)

                                                                                 cATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCt                                                                                                                                                                                    >  2:542251/1‑206 (MQ=255)

                                                                                 cATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACCCATGATTTCCAACATggg                                                                                                                                                    >  2:852029/1‑236 (MQ=255)

                                                                                 cATAAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGa                                                                                                                                                   <  2:1051379/239‑1 (MQ=255)

                                                                                  aTCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGa                                                                                                                                                   >  2:204089/1‑238 (MQ=255)

                                                                                    cAATTT‑TGCCAA‑GAACCCACTTGCCACGCCCTTCTT‑CACGCGCATACC‑GATCACGATACAACAATAATGCGGCAGTCCCGGGGCTGACCGCACATGCTGCCCCCAGCGATGCAAGGATCGATTCCCCACGCAGAGCCAACTCGC‑TTAACAACAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATcg                                                                                                                                                <  1:285375/239‑1 (MQ=255)

                                                                                           tGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCCC‑AAAAATCAATGCCAG‑ACCCCCGTGGGGGCCTAAACGGCAATCACGCAAGATTTCCAAAATGGGAtacgggcaa                                                                                                                                          >  1:1051379/1‑231 (MQ=255)

                                                                                              cAATGAACCCA‑TTGCCAAGCCCTTCCT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGt                                                                                                                                       <  2:1015076/239‑1 (MQ=255)

                                                                                                 tGAACACA‑TTGCCAAGCCCTTCGT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGGCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCa                                                                                                                                    <  2:637809/239‑1 (MQ=255)

                                                                                                    aaccactcgCCAAGCCTGTCTT‑CCCGCCAACAAC‑TATGACGTGAATTCAAAAATGGGGAATGCCCGCGCGTGACCGCACGTGCGGCCGCCCGCGATCCAAGGACCGATTCGCCACGCAGACCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTCCCAC‑AAAAAGCAATGCCAG‑ACCGCCGTggg                                                                                                                                                                                         <  1:1225200/176‑1 (MQ=255)

                                                                                                       ca‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACCCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAA‑ACCGCCGTGGGGGCCTAAACGGCTATCACCCATGATTTCCAACATGCGATCGCGGCACCGTCCAGAACAt                                                                                                                              >  2:1031415/1‑239 (MQ=255)

                                                                                                             ccAAGGCCTTCTTCCCCGCGCATAAC‑GATGCCGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGc                                                                                                                          <  2:190173/239‑1 (MQ=255)

                                                                                                              cAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATc                                                                                                                                                                         >  2:16089/1‑190 (MQ=255)

                                                                                                              cAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGGGGGTGCCTAAACg                                                                                                                                                                               >  2:1225200/1‑184 (MQ=255)

                                                                                                                  cccTTCTC‑CCCGCGCATAAC‑GATGACGAGACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATcac                                                                                                                    <  2:213242/239‑1 (MQ=255)

                                                                                                                     ttctt‑cCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCCC‑AAAAATCAATGCCAG‑ACCGCCGTAGGGGCATAAAAGGCTATCACGCAGGATTTACAACATGCGATCGCGGCTACGTGCAGaaaaacagccaacaaagg                                                                                                                 >  1:838298/1‑225 (MQ=255)

                                                                                                                     ttctt‑cCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCACCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAACCAGCTAACACGCATAAATTCCAAAAAGCGAACGCGGCAACggtcgaaaaaaacccaaacaaaga                                                                                                                 >  1:477023/1‑216 (MQ=255)

                                                                                                                      tctc‑cCCGCGGCTAAA‑AATGACGATACAGAATGAATGCGGGCGGCCCGCGGTTGACCACCCTAGCGTCCGCCAGCGATGAAAGGTTCGATTCGCCACGAATAGCCAACCCGC‑TTAACACCAGAATCTTTGCCG‑GTACCAC‑AAAATCCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGt                                                                                                                <  1:306651/239‑1 (MQ=255)

                                                                                                                       ctt‑cCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCAA‑GTACCAC‑CAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCCTGCTTTCCAACATGCGATCGCGTCTAAGTGCAGAAAATACGCCATCACAGGTa                                                                                                               >  2:891734/1‑239 (MQ=255)

                                                                                                                             cgcgCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCAACAACAGGCATCacg                                                                                                          >  2:51477/1‑237 (MQ=255)

                                                                                                                             cgcgCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCAAGCAGAGCCAACTCGC‑TTAACACACGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAA‑ACCGCCGCGGGAGCCTCAACGGCCTTCAACCCAGATTTCAAAAAGGCGATCCCGGCCACGTGCAGGAAAAAACCCATCCCAGGAAtaaac                                                                                                          >  1:11127/1‑235 (MQ=255)

                                                                                                                              gcgcATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACCCATGATTTCCAACCAACAATCCGGGCTACGCGCAAAAAATACCACCTCACAgggaaacgag                                                                                                         >  1:1271224/1‑231 (MQ=255)

                                                                                                                                        gaagacgcaccttttataatGGGGCTGGCCCGCGGCTGCCCCCCCCTGCGGCCGCCAGCGATGCAAGGATCGACTCGACACGCAGAGCCAACTCGC‑GTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAAGCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTcc                                                                                                <  1:458500/224‑1 (MQ=255)

                                                                                                                                          taacgctcactaaatattGGGGCAGGCCCGGGGCCGCCCCCCAAGGCTGCCCCCTGCGAGGCAAGGAACGATTCCACACCCAGAGCCAACTCGC‑TTAACCCCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTATACGGCTATCTCGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAg                                                                                              <  1:561746/227‑1 (MQ=255)

                                                                                                                                           taCGATTCATCAATAATGCGGCAGTCCCGGGGGTGACCGCACAGGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACGCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGa                                                                                             <  2:1242422/238‑1 (MQ=255)

                                                                                                                                               aTACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGa                                                                                         >  2:123015/1‑239 (MQ=255)

                                                                                                                                                       ataatGCGGCAGTCCCCCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAAAATC‑TTGCCA‑GTACCCC‑AACAATCACTGCCAG‑ACCGCCGTGGGTGCCTAACCCGCTATCACCCATGATTTCCAACCTGCGCTCGCCGCTACCTCCCGAAAATACGCCCACCCAGGCATCAGCAAGGCCCTCCAGATGGAAGcccccc                                                                                 >  1:391335/1‑237 (MQ=255)

                                                                                                                                                       aatatggcgatctccccgctgcgACCGCACATGCTGCGACCCGCGATCCAAGGATCGATTCGCACCGCAGAGCCAACTCGC‑TTAACACCAGAATCTGTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑AGCGGCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCAGTCCAGATGGAATCCCCCg                                                                                 <  1:129855/217‑1 (MQ=255)

                                                                                                                                                        taatGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCCGAAAATACGCCATAAAAGGTATCAGCAAGGCCGTCCAGATGGAATCCCCCg                                                                                 >  2:21816/1‑238 (MQ=255)

                                                                                                                                                        taatGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGGATCAGCGAGGCCGTCCAGATGGAATCCCCCg                                                                                 >  2:133462/1‑238 (MQ=255)

                                                                                                                                                        taatGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATCCGCTCGCGGCTACGTGCCGAAAAAACGCCATCCCAGGTATCACCGAGGCCGCCCAGAGGGAACCCCCCg                                                                                 >  1:253799/1‑238 (MQ=255)

                                                                                                                                                           tGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAACCGGCTATCACCCATGATTTCCAAAATGCGATCCCGGCTCCGTGCAGAAAATACCCCATCACACGACTCAACGAGGCCGTCCAAATGGAATccccccgccc                                                                             >  1:529328/1‑234 (MQ=255)

                                                                                                                                                             cGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCCGAATCTTTGCCA‑GTACCAC‑AACAATCAATTCCAG‑ACCGCCGTGGGGGCCTAAACGGCTCACACGCATGCATTCCAACATGCACTCGCGGCAACGTGCATAACCTACGCCCTCACAGGTCACAGCGAGGCCGGCAACATGGCAACCCCCGA‑ccatc                                                                          >  1:103515/1‑239 (MQ=255)

                                                                                                                                                             cGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGCTTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCCACGGACTCAGCAAGGCGGCCCGAATGGAAACCCCCgaccata                                                                           >  1:883070/1‑233 (MQ=255)

                                                                                                                                                             cGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATCCGATCGCGGCTACGTGCAGAAAATCCGCCATCACTGGAATCTGCGAGGCCGTTCAGATGGAATTCCCCGG‑taatc                                                                          >  2:888766/1‑239 (MQ=255)

                                                                                                                                                                  gTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATaagca                                                                      >  2:1071664/1‑238 (MQ=255)

                                                                                                                                                                  gTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCAAGGCCGTCCAGATGGAATCCCCCGG‑TCATcagca                                                                      >  2:1183217/1‑238 (MQ=255)

                                                                                                                                                                   tCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATcagcac                                                                     <  2:416054/238‑1 (MQ=255)

                                                                                                                                                                     ccGGGGGTGGCCGCCAATGCTGGCGCCAGGGATGCAAGGATCGATTCGCCACGCAGAGCCAAATCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATcagcaccag                                                                  <  2:883070/239‑1 (MQ=255)

                                                                                                                                                                          gCTGCCCGCACATGGTGGCCCCAGCGAGCGAAGGATCGATTCGCCACGCAAACCCAACCCCC‑TAAACACCGGAATCTTTGCCA‑GGACCGC‑AAAAATAAATGCAAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGAGTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACaa                                                             <  1:688281/239‑1 (MQ=255)

                                                                                                                                                                          ctcgACCCCACATGCGCGCCCCAGCGATGCAAGGATCTATCACCCCGGCAGACCCAACCCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAA‑AAAAATCAATGCCAG‑GCCGCCGTGGGTGCCTAAACGGCTAACACGCATGATTCCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACaa                                                             <  1:455402/236‑1 (MQ=255)

                                                                                                                                                                          ccTGCCCGCCCATGCGCCCGCCAGCGATGAAAGGATTGAATCGCCACGAAGGACCAACCCGC‑TTAACCCCAGAATCTTGCCCA‑GTCCCAA‑AAAAATAAATGCCAG‑GCCGCCGTGGGGGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGcacag                                                             <  1:1311467/238‑2 (MQ=255)

                                                                                                                                                                           tggaccggctgtgcggcCGGCAGCTATGCAAGGATCGATTCGCCACGCAGAGCCAACGCGG‑TTAACACCAGAATCTTTGCCA‑GTCCCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGCAATCCCCCGG‑TCATCAGCTCCAGCACaa                                                             <  2:221790/227‑1 (MQ=255)

                                                                                                                                                                           tggACCGCACAGGCGTCCGCCAGCGATTGATGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACaa                                                             <  2:525753/236‑1 (MQ=255)

                                                                                                                                                                           ctacccGCACATGCCGCCGCCAGCTATGCAAGGACCGAGTCGCCACGCAGAGCCAACGCGC‑TTAACCCCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGTCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACaa                                                             <  2:11127/234‑1 (MQ=255)

                                                                                                                                                                           ccgACCCCCCATGCGGCCGCCAGCCATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGT‑TTAACCCCAGAATCTTTGCCA‑GTACCAC‑AAAAACCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACaa                                                             <  2:1291725/236‑1 (MQ=255)

                                                                                                                                                                           cTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCCCGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACaa                                                             <  2:253799/238‑1 (MQ=255)

                                                                                                                                                                           cTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGACACGCAGAGCCAACTTGC‑TTAACACCAGAATCTTTTCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACaa                                                             <  2:260956/238‑1 (MQ=255)

                                                                                                                                                                           atgtccGCCCATGCTGCCGCCGACGATGCAAGGCTCGATTCGCCACCCAAGGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑ATACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACaa                                                             <  2:938744/234‑1 (MQ=255)

                                                                                                                                                                            ttcccccacaTCCTGCCCCCGGCGATGCAAGTATCGATTCCCCCCGCAAAGCCAACCCAC‑TTAACACCAGAATCTTTGCCA‑GTACAAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACAAGt                                                           <  1:167477/233‑1 (MQ=255)

                                                                                                                                                                              aCCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGCGCAGAAAATACGCCATCACAGGGATCAGCGAGGCCGCCCAGATGGAATCCCCCggcactcaccacacccccaagta                                                           >  1:1060070/1‑217 (MQ=255)

                                                                                                                                                                                aaagagccaggccgccAGCGATGCAAGGACCGATTCGCCACGCAGGGCCAACTCGC‑TTAACCCCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACAAGTACg                                                        <  2:903514/228‑1 (MQ=255)

                                                                                                                                                                                        ctgcCCCCAGCGATGCAGGGTTCCACTAGCACCACA‑AGGCAAAACGCATTCACCCCATAATCTTTGCCA‑‑TACCCCAAAAAATCATTGCCAA‑CGCGCCGGGGGTGCCTAAACGGCGACCACCACTTATTTCGAACATGCCATCGCGGCTACGTGCAGAAAACACGCCACCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACAAGTACGCTAAACAGt                                               <  1:472900/239‑1 (MQ=255)

                                                                                                                                                                                        cggcagccAGCGATGCAAGGATGGATTCGCCACGCAGCGCCAACGCGC‑TTAACACCAGAATCTTTTCCA‑GTACAAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACAAGTACGCTAAAc                                                  <  2:1013887/231‑1 (MQ=255)

                                                                                                                                                                                               cAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACCCCATCACAGGTATCAGCGAGGCCGTCCCGATGGAATCCCCCGG‑CCACCACAACCACCaaaaaaaagccaaaaaaggccccaa                                        >  1:631810/1‑218 (MQ=255)

                                                                                                                                                                                                   gATGCAAGGACCGATTCCCCACGCAGAGCCAACTCCC‑TTAACACCATAACTTTTGCCA‑GTACCCA‑AAAATTCAATGCCAG‑CCCGCCGTGGGTGCCTAAACGGCTAACACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACAAGTACGCTAAACAGTGCCGCCAg                                       <  2:528982/236‑1 (MQ=255)

                                                                                                                                                                                                     tGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTCCGTGCAGAAAATACGCCATCACAGGGATCAGCGAGGCCGTCCAGTGGGAATCCCCCGG‑CCATCAGAACAAGCAAAAGTACGCTaacacgggccgtccccttta                                  >  1:903514/1‑221 (MQ=255)

                                                                                                                                                                                                     tGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGGATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑CCATCAGCACCAGCAAAAGTACGCTAAACAGTGCCGCCAgtggt                                   >  2:570688/1‑238 (MQ=255)

                                                                                                                                                                                                            aTCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATca                                                                                                                     >  2:407549/1‑150 (MQ=255)

                                                                                                                                                                                                               gATTCGCCACGCAGAGCCATCTCGC‑TTAACACCAGAATCTTTGCCC‑GTACCAC‑AAGAATCAATGCCAG‑ACCGCCGTGGGCGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGAGCAGAAAATAAGCCATCACCGGTACCAGCGAGTCCGTCCAGCTGTAATCCCCCGGCTCTCCAG‑ACCAGACCAAGCACGCCAAACCGTAACCCCCCTGGTGCGcccacccc                        >  1:606517/1‑233 (MQ=255)

                                                                                                                                                                                                                   ccccACGCCAAGCCAACCCCC‑TTAACCCCAAAATTTTTGCCATGTA‑CAC‑AAAAATGAATGCCAG‑ACCGCCGTGGGGGCCAAAACGGCTAGCCCGCATGAGTTCCAACATGCGATCGCGGATACGTGCAGAAAATACGCCATCACAGGTAGCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACAAGTACGCTAAACAGTGCCGCCAGTGGTGCGCCACACCATGcc                    <  1:204089/237‑1 (MQ=255)

                                                                                                                                                                                                                     ccccccAGACCCAACTCGC‑TTACCCCCTAGATCTTTCCCA‑TCACC‑C‑AAAAAACGAATCCAGTACCCCCTGGGGTGCCTAAACGACTATCACGCATTATTGCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGACTCCCCCGG‑TCATCAGCCCCAGCACAAGTACGCTAAACAGTGCCGCCAGTGGTGCGCCACACCATGCCTg                  <  1:364350/234‑1 (MQ=255)

                                                                                                                                                                                                                     ccACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCACCAGCCCCAGCAAAAGCACGCTAAACAGGGCCGCCAGTGGTGCCCCACACCACACCTg                  >  1:908151/1‑239 (MQ=255)

                                                                                                                                                                                                                        cGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACAAGTACGCTAAACAGTGCCGCCAGTGGTGCGCCACACCATGCCTgca                >  2:416850/1‑238 (MQ=255)

                                                                                                                                                                                                                        cGCAGAACCAACTCGC‑TTAACCCCAAAACCTTTGCCA‑GGCCCCA‑AAAAACCAATGCCAG‑GCCGCCGTGGGGGCCTAAACGGCTATCCCGCATGATTTCCAACAGGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACAAGTACGCTAAACAGTGCCGCCAGTGGTGCGCCACACCATGCCTgcag               <  2:362343/239‑1 (MQ=255)

                                                                                                                                                                                                                         gCAGGGCCAACTCGC‑TTAACCCCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTAACACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACAAGTACGCTAAACAGTGCCGCCAGTGGTGCGCCACACCATGCCTgcagc              <  2:847453/239‑1 (MQ=255)

                                                                                                                                                                                                                           tcggcacaaccgctttAACCACAGAATCTTCTACA‑ATCCCAG‑CAAAAACAAGGCCAG‑CCCGCCGTGGGTGCCTAAACAGCTATCACGCATGATTTCCAACAAGCGAGCGCGGCTACGTGCAGAAAATACCCCATCACAGGTATCAGCGAGGCCGTCAAGATGGAATCCCCCGG‑TCATCAGCACCAGCACAAGTACGCTAAACAGGGCCGCCAGTGGTGCGCCACACCATGCCTgcagca             <  1:1105522/225‑1 (MQ=255)

                                                                                                                                                                                                                                   cTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCCCAAGTACGCTAAACAGTGCCGCCAGTGGTGCGCCCCACCATGCCTGCAGCACCACGAAg     >  2:1001400/1‑238 (MQ=255)

TAGCAGCCACACCATGCATACCCGGCAATAATACGGCAGCAAAAATAGCGGCTAAGCCGAGTGTGACGCAGGTTTGTCGCCCATCAATTT‑TGCCAATGAACACA‑TTGCCAAGCCCTTCTT‑CCCGCGCATAAC‑GATGACGATACATCAATAATGCGGCAGTCCCGCGGCTGACCGCACATGCTGCCGCCAGCGATGCAAGGATCGATTCGCCACGCAGAGCCAACTCGC‑TTAACACCAGAATCTTTGCCA‑GTACCAC‑AAAAATCAATGCCAG‑ACCGCCGTGGGTGCCTAAACGGCTATCACGCATGATTTCCAACATGCGATCGCGGCTACGTGCAGAAAATACGCCATCACAGGTATCAGCGAGGCCGTCCAGATGGAATCCCCCGG‑TCATCAGCACCAGCACAAGTACGCTAAACAGTGCCGCCAGTGGTGCGCCACACCATGCCTGCAGCACCATGAAGACC  >  NC_000913/2064610‑2065073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`