Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,074,021 A→C 13.3% Y828S (TAC→TCC)  flu → CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,074,0210AC13.3% 169.8 / 3.3 66Y828S (TAC→TCC) fluCP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
Reads supporting (aligned to +/- strand):  ref base A (47/8);  new base C (3/8);  total (50/16)
Fisher's exact test for biased strand distribution p-value = 2.48e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCATCTCGGTCACGATAACAATGGCGGTATTGCCCGTGGGGCCACGCCGGAAAGCAGCGGCAGCTATGGATTCGTCCGTCTGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACGCCGGTTA  >  NC_000913/2073790‑2074258
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 
gtcccgtcGGTCCCGTTAAAAATGGGGGTTTTGCCCGTGGGGCCCGGCCGGAAAGCAGCGGCAGGTAGGGATTCGTCCGTCTGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAAt                                                                                                                                                                                                                                            <  1:607726/233‑1 (MQ=255)
 gcctccgcgTCACGATAACCATGGCGGTATTGCCCGTGGGGCCAGGCCGAAAAGGAGCGGCAGGTATGGCTTGGTCCGTCGGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGGCCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAAt                                                                                                                                                                                                                                            <  1:261346/230‑1 (MQ=255)
           cACGATAACAATGGCGGTATTGCCCGTGGGGCCACGCCGGAAAGCAGCGGCAGCTATGGATTCGTCCGTCTGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGGATATGGTGCTGCTGGCCATTCTTCCGTTTATGTTAAGGATGATGACGGCTCCCGTGCCGGCCCGGGCCGGGGTGATGCCGGCAGCCTGGGCGGGACCCTGAAACTGGTaccac                                                                                                                                                                                                                                  >  2:1216709/1‑235 (MQ=255)
                  aCAATGGCGGTATTGCCCGTGGGGCAACGCCGGAAAGCAGCGGCAGCTATGGATTCGTCCGTCTGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGtcctcc                                                                                                                                                                                                                          <  2:1150821/239‑1 (MQ=255)
                       gggggttgtGCCCGGGTGGCCACGCCGGAAAGCAGCGTGAGCTATGGATTCGTCCGTCGTGAGGGTGACCTGATGAGCACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATTACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCt                                                                                                                                                                                                                     <  2:1229799/231‑1 (MQ=255)
                            tATTGCCCGTGGGGCCACGCCGGAAAGCAGCGGCAGCTATGGATTCGTCCGTCTGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTgg                                                                                                                                                                                                                 <  2:430521/238‑1 (MQ=255)
                                gCCCGGGGGGCCACGCCGGAAAGCGGCAGCAGCTATGGATTCGTCCGTCTGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGa                                                                                                                                                                                                            <  2:614428/239‑1 (MQ=255)
                                     tGGGGCCACGCCGGAAAGCAGCGGCAGCTATGGATTCGTCCGTCTGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGACCACACGTCCTCCGGCCTGTGGGCTGACATtg                                                                                                                                                                                                       >  2:466273/1‑239 (MQ=255)
                                                  gAAAGCAGCGGCAGCTATGGATTCGTCCGTCTGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCCCAGGGaa                                                                                                                                                                                           >  2:362717/1‑238 (MQ=255)
                                                                          gtccgtcTGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGCCATTTTGGCCCAGGGAACCCGCCCCAGCATGAAAGCGTAATc                                                                                                                                                                >  2:253200/1‑239 (MQ=255)
                                                                          gtccgtcTGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAACGCCATc                                                                                                                                                                >  2:1151513/1‑239 (MQ=255)
                                                                          gtccgtcTGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGGGGCACAGGGAACCCGCCACAGCATGAAAGCGTCacc                                                                                                                                                                >  2:1297291/1‑237 (MQ=255)
                                                                          gtccgtcTGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCATCCGGCCTGTGGGCGGACATTGTGGCACAGGGAACCCGCCCCAGCATGAACGCGGCATc                                                                                                                                                                >  2:750738/1‑239 (MQ=255)
                                                                                     gggTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACCCGTCCTCCGGCCTTTGGGCTGACCTGGTGGCACCGGAAACCCGCCACAGCATGAAAACGTCACCGGACAATAACg                                                                                                                                                     >  2:1246747/1‑239 (MQ=255)
                                                                                        tGACCTGATGCGTACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGCTGATGACGGCTCCCGTGCCGGCTCGGTCCGGCATGATGCCGGCAGCCTGTGCGGCTCCCTGAATCTGGTCCACGAGTCCTCCGGTCGGCGGGTCGGCATTCAGGCACCGGGGAACCGCCCCCGCCTGAAAGCGGCCTCgcaaaaaaaaaca                                                                                                                                                   >  1:1100817/1‑226 (MQ=255)
                                                                                        tGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGAGGATGACGGCTCCCGTGCCGGCACGGGCCGGCGTTATGACGGCAGACTGGGCGGCTACCTGAATCGGGAACACACATCCTCCGGCCTGCAGGCTGACCTTGTGGCACAGGGCCCCCCCCCCCCCATGAAAGCAGCCTCGGACAATAACGaa                                                                                                                                                   >  1:942765/1‑237 (MQ=255)
                                                                                                tgaGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTcccggc                                                                                                                                           >  2:1096356/1‑234 (MQ=255)
                                                                                                         agGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGGTGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCGGAATCTGGAACACACGTCCTCCGGCCTGGGGGGTGCCATTGTGGCACAGGGAACCCGCCCACGAATGAAAGAGTCATCGGACAAAAACAAATTCCCCGCCCggggcggg                                                                                                                                 >  1:1099792/1‑239 (MQ=255)
                                                                                                         agGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCGGACATTGTGGCACAGGGAACCCGCCACAGCATGa                                                                                                                                                                           >  2:1011254/1‑198 (MQ=255)
                                                                                                              gCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGCACTCGGACCATAACGACTTCCGCGCCCGgggctggggctg                                                                                                                            >  2:1217050/1‑239 (MQ=255)
                                                                                                                         tgtgACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCCGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGGACACACGTCCTCCGGCCTGCGGGCCGACATTGTGGCCCACGGAACCCGCCACCGCCGGCAAACGGCATCGGACCATAACGAACTCCCCGCCCggggcccggaccggtcgggccgac                                                                                                                 >  1:1056089/1‑220 (MQ=255)
                                                                                                                                 cgGGGGTATATGGTGTTGCTGGCCATTCTTCCGTTGATGGTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGCATCTGGTCCCCACGCCATCCGGCCTGTGGGCTGACCTTTTGGCACAGGGAAACCGCCACACACGACAAGCGGAATCGGCCAATACCGCCATCCGCGCCCcgggctggtgcgtcctggcgccgcctgaaaa                                                                                                          >  1:1199810/1‑218 (MQ=255)
                                                                                                                                 cgGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGa                                                                                                                                                                                                                                              >  2:296502/1‑108 (MQ=255)
                                                                                                                                 cgGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGa                                                                                                                                                                                                                                              <  1:296502/108‑1 (MQ=255)
                                                                                                                                 cgGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCCCGCCCGGGGCTGGGGCTGGCTGGGGTCACTGGAAAcc                                                                                                        >  2:519149/1‑239 (MQ=255)
                                                                                                                                   ggggTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCCCAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCg                                                                                                       >  2:857574/1‑238 (MQ=255)
                                                                                                                                              gactgctgGCCAGTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGTAAGCCTGGGCTGTTCACTGAATCTGGTACCCCCGTCCTCCGGCCTGTAGCGTGGCCTTGGTGCCCAAGGAACCCCCACCGTCCTGACAGCGTCATTGGACAATACCGACTTCCGCCACCGGGGCTGGGCCGTCCTGGGCCCACtcaaaaagagaccgccctgca                                                                                           >  1:886275/3‑219 (MQ=255)
                                                                                                                                                ctgctgGCCATTCTTCCGTTGCTGTTAAGGATGCTGACGGCTCCCGTGCAGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGCTACCTGATTCTGGTACTCCCGTCTTCCGGCCTGGGGGCTGACATTGTGTCACCGGAAAACCGCCACCGCATGTCAGGTCCATTGGCCACTACAGCCTTCCGCGCCCGGGGCTGGGCCGGGCCGGGCACACGTGGaacaccatacacctcccga                                                                                          >  1:200571/1‑222 (MQ=255)
                                                                                                                                                ctgctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGc                                                                                                                                                                                                               >  2:965833/1‑124 (MQ=255)
                                                                                                                                                ctgctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGGAACCGGGCTGCCCTTCAGt                                                                                         >  2:744703/1‑239 (MQ=255)
                                                                                                                                                ctgctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGt                                                                                         >  2:61627/1‑239 (MQ=255)
                                                                                                                                                ctgctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCGCCCGTGCCGGAACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAGTCTGGCACACACGGCCTACGGACTGGGGGCTGCCATTGGGGCACAGGGAACCCGCCACACCCTTAACAGGCCATCGGACAATAGCGACTTCCGAGCCCTGGGCCGGGGCAGGCCGGGGGCCGTGGAAACCGGGCGGCCCtgtagg                                                                                         >  1:1325429/1‑234 (MQ=255)
                                                                                                                                                ctgctgGCCAGTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGc                                                                                                                                                                                                               <  1:965833/124‑1 (MQ=255)
                                                                                                                                                ctgctgGCAATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCCCAGGGAACCCGCCACAGCATGAAAGCGTCATCGGGCAATAACGACTTCCGAGACCGGGGCTGGGGCTGGCTGGGCTCACTGGAAAccctgtctcttataac                                                                                          >  2:321834/1‑224 (MQ=255)
                                                                                                                                                   ctgGCCATTCTTCCGTTGATGTTAAGGATGCTGACGGCTCCCGTGCCGGCACGGTACGGGATGATTCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGCAATTGTGGCACAGTGGAACTGCCACAGAATCAAACCGTGAACGGACAATACCGAATTCCCCGCCCGCGGCCCGGGCTGCGCGGGCTCCCTTGAAAcccatcgccccctcagaaca                                                                                      >  1:1280926/1‑221 (MQ=255)
                                                                                                                                                   ctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACCGGGAACCCGCCCCAGCCTGAAAGCGGCACCGGCCAATAACAACTTCCCCGCCCGGGGCCGGGGCGGGCCGGGCCCACTGAAAACCggccctccccccagagt                                                                                       >  1:1152584/1‑223 (MQ=255)
                                                                                                                                                   ctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCCTGAAAGCGTCATCGGCCAATAACGACTTCCGCGCCCCGGCCTGGGGCGGGCTGGGCTCACTGGAAACCGGTCTGCCCTCCAGGAt                                                                                       >  1:894823/1‑238 (MQ=255)
                                                                                                                                                   ctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGCCAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCCCACTGGAAACCGGTTTGCCCTTCAGTAt                                                                                       >  2:568496/1‑238 (MQ=255)
                                                                                                                                                   ctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCCCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAgatc                                                                                       >  2:476563/1‑235 (MQ=255)
                                                                                                                                                   ctgGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGGCATCGGACCATAACGACTTCCGCGCCCGGGGCTGGGCCTGGCTGGGCCCACTGGAAACCGGTCTGCCCTTCAGTATc                                                                                      >  2:566596/1‑239 (MQ=255)
                                                                                                                                                      gCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGGCATTTTGGCACAGGGAACCCGCCACAGCCTGAAAGCGTCCTCGGACCATAACGACTTCCGCCCCCGGGGCTGGGCATGCCTGGGCTCACTGGAACCCGGTCGCCCCTTccggtcaacc                                                                                   >  1:967356/1‑230 (MQ=255)
                                                                                                                                                        cATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCGGGCATTTGGGCACAGGGAACCCGCCACAGCATGAAACCGGCATCGGACAATACCGCCTTCCACGCCCGGGGCTGGGGCTGGCTGGGCCCCCCGGAAACCGGTCTGcccccaaggacacctga                                                                                 >  1:35425/1‑225 (MQ=255)
                                                                                                                                                                             gtggatggggccccccggcccGGCGCGGTCGGGGAGGGCGCCGGCAGCGGGGGCGGATCCATGAATCTGGTACACACGTCCCCGGGCTTGTGGGATGACATTGTGCCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCcac                                                            <  2:1099792/219‑1 (MQ=255)
                                                                                                                                                                                gatgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACGGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATCCTTGAGCCCCAAcg                                                        >  2:920844/1‑238 (MQ=255)
                                                                                                                                                                                 atgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGCCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGCCAACCTGATGCTGGAGCCACAACTg                                                       >  2:183881/1‑239 (MQ=255)
                                                                                                                                                                                  tgaCGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCCGGGGCTGGCTGGGCTCACTGGAAACCGTGCTGCCCTTCAGAATCACTGACAACCTGATACTGGAGCCACAACCGc                                                      >  1:1157307/1‑239 (MQ=255)
                                                                                                                                                                                    ggggCTCCCGGGCCGGCCGGGTGCGGGATGATGGCGGCACGCGGGGCGGATCCCGGAACCTGGTCCACCGTTCCCCGGGCGGGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCa                                                     <  2:481866/236‑1 (MQ=255)
                                                                                                                                                                                        cTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACCCACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCCCAACTGAGGtat                                                 >  2:1084984/1‑238 (MQ=255)
                                                                                                                                                                                         ccccGTGCCGGCACGGTCCGGGATGAGGCCGGCAGCCGGGGGGGATCCCTGAATCTGGTACACCCGTCCCCCGGCCTGTGGGCTGACTTGGGGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATAc                                               <  2:1152584/238‑1 (MQ=255)
                                                                                                                                                                                               gccggccgGGTCGGGGATGATGCCGGCACCCTGGGCGAATCCCTGAATCTGGACCCCACGCCCGCGGTCTTGGGGGTTAAAATGGGGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGc                                          <  2:35425/238‑1 (MQ=255)
                                                                                                                                                                                               gCCGGCACGGGCGGGAATGGGGCCGACAGCTGGGCGGGATCCCTGATCCGTGTACACAGGTCCCCCGGCTTGGGGGCTGACATTGTGGCACAGGGACCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGc                                          <  2:843427/238‑1 (MQ=255)
                                                                                                                                                                                                   gCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTCCCGTATACCTGGCAggg                                      >  2:427716/1‑238 (MQ=255)
                                                                                                                                                                                                              gatgatGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGCCTGCCCTTCGGTATCACTGGCAACCTGATGCCGGAGCACCAACTGCGGGATACCTGGCAGGGGCTTTCCCTGGa                          >  1:768202/1‑239 (MQ=255)
                                                                                                                                                                                                               aggaggCCGGGACCCGGGGCGAATCCCGGAACCGGGAACAACCGTCCTAGGGCCTGTGGTGTGACTTGGGGGCAGGGGGACCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGa                          <  2:138118/233‑1 (MQ=255)
                                                                                                                                                                                                                  atgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCCGTATCCCTGACAACCTGATGCTGGAGCCACAACTCCAGGTAACCTGGCAGGGCCTTTCCCCGGGTGag                      >  1:1251795/1‑238 (MQ=255)
                                                                                                                                                                                                                   tgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCCCTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCCGATGCTGGAGCCACAACTGCAGTATACCTGCCAGGGACTTTCCCTGGATGACg                     >  2:549897/1‑239 (MQ=255)
                                                                                                                                                                                                                   tgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCCCGGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACg                     >  2:996829/1‑239 (MQ=255)
                                                                                                                                                                                                                   tgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGtata                                                >  2:717872/1‑212 (MQ=255)
                                                                                                                                                                                                                   tgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCGGGGCTCACTGAAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCCGTATACCTGGCAAGGACTTACCCAgggggagg                     >  1:1200173/1‑233 (MQ=255)
                                                                                                                                                                                                                   tgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTACCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTGTGCCCTTCAGTCTCCCTGGCAACCTGCTGCTCGGGCGCCAAACGCAGGAGACCTGGCCGGGACGTTCCCCGGATGACg                     >  1:115343/1‑239 (MQ=255)
                                                                                                                                                                                                                   tgCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCACCCCAGCATGAAAGCGTCATCGGAAAATAACGACTTCCGCGCCCGGGGCTCGGACTGGCTGGGCCCACTGGCAACCGGTCTGACCTTCCGGATTCCTGACAACCTGATGCCGACGGCCCCACGCCAAGATACCTGGCCGGGCCTTTACCTgtgggcgg                     >  1:89628/1‑232 (MQ=255)
                                                                                                                                                                                                                   tgCCGGCAGCCGGGGCGGATACCTGAATCGGGTCCACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCGGGGGCTGGCGGGGCTCACTGGAACACGGTCTGCCCTTCAGTATACCTGCCAACCTGATGCTGGGCCACCCACGCCACTATACCTGCCAGGCGCTTTCCCCGGATGaag                     >  1:933513/1‑237 (MQ=255)
                                                                                                                                                                                                                        gCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCGGGCTGGGCTCACTGGAAACAGGTCTGCCCTTCAGTATCAATGCCAACCTGTTGCTGGAGCCACAACTCCCGAATACCGTGCAGGGGACTTCCCCGGATGAAGGAAAg                >  1:297838/1‑239 (MQ=255)
                                                                                                                                                                                                                               ggggggATCCCTAAACTTGGTACCCCCGTCCCCGGGCCTGGGGGCTGACTTGGGGGCACAGGAACCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAAc          <  2:1251795/238‑1 (MQ=255)
                                                                                                                                                                                                                                 gCGGATCCCGTAATTTGGTACACCCGCCGTCGGGCTGGGGGGGTGACTTGGTGGCACGGGGACCCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACGc        <  2:942765/238‑1 (MQ=255)
                                                                                                                                                                                                                                       tACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGCGGAGGGAAAGGACAAAACCGGGTa  >  1:1196786/1‑239 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 
GTCATCTCGGTCACGATAACAATGGCGGTATTGCCCGTGGGGCCACGCCGGAAAGCAGCGGCAGCTATGGATTCGTCCGTCTGGAGGGTGACCTGATGAGAACAGAGGTTGCCGGTATGTCTGTGACCGCGGGGGTATATGGTGCTGCTGGCCATTCTTCCGTTGATGTTAAGGATGATGACGGCTCCCGTGCCGGCACGGTCCGGGATGATGCCGGCAGCCTGGGCGGATACCTGAATCTGGTACACACGTCCTCCGGCCTGTGGGCTGACATTGTGGCACAGGGAACCCGCCACAGCATGAAAGCGTCATCGGACAATAACGACTTCCGCGCCCGGGGCTGGGGCTGGCTGGGCTCACTGGAAACCGGTCTGCCCTTCAGTATCACTGACAACCTGATGCTGGAGCCACAACTGCAGTATACCTGGCAGGGACTTTCCCTGGATGACGGTAAGGACAACGCCGGTTA  >  NC_000913/2073790‑2074258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: