Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,120,560	A→C	10.8%	V332G (GTG→GGG)	wcaJ ←	colanic biosynthesis UDP‑glucose lipid carrier transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,120,560	0	A	C	10.8%	202.1 / 2.0	90	V332G (GTG→GGG)	wcaJ	colanic biosynthesis UDP‑glucose lipid carrier transferase
Reads supporting (aligned to +/- strand):  ref base A (54/24);  new base C (4/8);  total (58/32)
Fisher's exact test for biased strand distribution p-value = 2.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAA‑CCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTC  >  NC_000913/2120328‑2120796                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 ggTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACCGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCa                                                                                                                                                                                                                                            <  2:689359/239‑1 (MQ=255)       gCCCGGTTTCCCCTTAGGGCGCAGCATGGAGCCTTCAATGAGCTGTCGATACTGTTCGTTAGGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATggaac                                                                                                                                                                                                                                      <  2:5266/239‑1 (MQ=255)             tttCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACt                                                                                                                                                                                                                                >  2:223633/1‑239 (MQ=255)                   cTTATGGCGCAGGATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCa                                                                                                                                                                                                                                            <  2:772202/221‑1 (MQ=255)                     tATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTTTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACt                                                                                                                                                                                                                        >  2:1074068/1‑239 (MQ=255)                       tGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGGGGTACG‑GCGCAGAAAGTTCCCCCC‑TTT‑GGGG‑ACGCGCGG‑ATC‑GTTCTGCGCCGCCTGGGGCACCCCCTTGTCGTTCTCCATCACTTTTATGACACGGAAATCCcaacattt                                                                                                                                                                                                                      >  1:498167/1‑233 (MQ=255)                       tGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATACCTTTCATGGAACGGAACTTCCACACttt                                                                                                                                                                                                                      >  2:1221454/1‑239 (MQ=255)                            cagcaTGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCg                                                                                                                                                                                                                >  2:483506/1‑239 (MQ=255)                             agcaTGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCCGAAAGTTCCCCAC‑TTT‑GGAG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTTTGGTTCTCCTCCCCTTTCAGggaacggaccctgcccctttaacaaa                                                                                                                                                                                                                 >  1:772202/1‑220 (MQ=255)                             agcaTGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑CCGCGGGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCg                                                                                                                                                                                                                >  2:243607/1‑238 (MQ=255)                                 tgtgGCCTTCAATGGGCTGGCGATACTGTTCGTTTTGCGCTACTGCGTGCGGACGGGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCAGCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑Tg                                                                                                                                                                                                          <  1:1244675/239‑1 (MQ=255)                                 tGTTGCCTTCAAGGTGCTGTCGATCCTGTTCGTTATGGGCTACCTGGGGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGCGGTACG‑GGGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGGCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑Tg                                                                                                                                                                                                          <  1:486595/239‑1 (MQ=255)                                         tCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCCCGG‑ATC‑GGTCTGCGTCGCCTGGGTCACCACTTTTTCGTTCTCCATCACTTTCCTGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGccacccc                                                                                                                                                                                                   >  1:1026724/1‑237 (MQ=255)                                                gCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGACGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGCCGCCTGGGTCACCACTTTGTCGTTCTCCATCCCTTTCCTGGCACGGAACTGCCACACTTTG‑CTCGGCT‑TTCCATCCATGCCGTAg                                                                                                                                                                                           >  1:968307/1‑239 (MQ=255)                                                  tGTCGATTCTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGc                                                                                                                                                                                          <  2:431286/238‑1 (MQ=255)                                                    tCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTT‑ATCGGCT‑TGCCATCCCTGCCGGAGCggg                                                                                                                                                                                       >  1:1067329/1‑237 (MQ=255)                                                    tCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGCCACCACTTTGTCGTTCTCCATCCCTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCCTACCGa                                                                                                                                                                                        >  2:719106/1‑238 (MQ=255)                                                           tGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGGGTCTGGCg                                                                                                                                                                                >  2:98778/1‑239 (MQ=255)                                                           tGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGCCg                                                                                                                                                                                >  2:815333/1‑239 (MQ=255)                                                            gTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GGTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTACGGGAACGGAACTTCCACACTTTG‑TTCGGCT‑TTccatccctaacgaagcaagagtgggag                                                                                                                                                                               >  1:119259/1‑218 (MQ=255)                                                              tCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑CTC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTT‑ATGGGCT‑TGCCATCCATGccctcgacggtcttgcgaaa                                                                                                                                                                             >  1:204094/1‑221 (MQ=255)                                                              tCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCCACCTTTG‑ATCGGCT‑TGCAACCCATTCCGTACCAGGTCCGGGGGaa                                                                                                                                                                             >  1:431954/1‑239 (MQ=255)                                                              tCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGaa                                                                                                                                                                             >  2:1081610/1‑239 (MQ=255)                                                              tCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGaa                                                                                                                                                                             >  2:395195/1‑239 (MQ=255)                                                              tCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGaa                                                                                                                                                                             >  2:11588/1‑239 (MQ=255)                                                              tCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGGG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGACCGGAACTTCCCCACTTTG‑ATCGGCT‑TGCCATCCATCCCGTAGCGGGTCTGGCggca                                                                                                                                                                             >  2:58519/1‑237 (MQ=255)                                                              tCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGGG‑ACCCGCGG‑ACC‑GTTCTGCGTGACCTGGGTCACCACTTTGTCGTTCCCCATCACTTTCATGGAACGGCACTTCCACCATTTG‑ATGGGGT‑TCccaccccccccgaacgagacaccggggaa                                                                                                                                                                             >  1:772017/1‑216 (MQ=255)                                                                gTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGcaaa                                                                                                                                                                           >  2:920757/1‑239 (MQ=255)                                                                  tATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTcc                                                                                                                                                                                                                             <  1:808181/189‑1 (MQ=255)                                                                  tATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTcc                                                                                                                                                                                                                             >  2:808181/1‑189 (MQ=255)                                                                        cTACTGCGTGCGGACGTGGACCGACAACCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCCC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCgg                                                                                                                                                                    <  2:119259/238‑1 (MQ=255)                                                                           cTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGGCGTTCTCCATCACTTTCATGGAACGGCATTTCCCCCCTTTT‑ATGGGCT‑TGCCACCCATGCCCAACCGAGTCCGGGGGCaaaaaaacagc                                                                                                                                                                   >  1:415871/1‑231 (MQ=255)                                                                            tggggggggACGTGGACCGACAATCGGCCTCCCCCCGGT‑CAGCAAATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCCC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTg                                                                                                                                                               <  2:1161429/232‑1 (MQ=255)                                                                              cgtgcgGACGTGGACCGCCAATCGACATCCCCCCGGT‑CAGCACATTGTTAAACTGCGGCA‑CTTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTC‑GGTG‑ACGTCCGG‑ACC‑GTTCTGCGTCGCCCGGGTCACCACTTTGTCGGTCCACATCACTGTCATGGCACGGAACTTCCAACCTTTT‑AT‑GGCTCTGCCATCCAACCCTCAACGAGTCTAGCAGAAAAAAAACCGcccatgc                                                                                                                                                             >  1:13201/1‑235 (MQ=255)                                                                              cgtgcgGACGTGGACCGACAATCGACATCCCCCCGGT‑CACCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑ACGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACACGCGG‑AGC‑GTTCTGCGTCGCCTGAGGCACCACTTTGTCGGTCTCCCTCACTTTCATGGCACGACAATTCCCCACTTTG‑ATCGGCT‑TGCAATCAATACCTGAGCGAGACTGGCGGAAAAAAAACGGCCCgggg                                                                                                                                                             >  1:655831/1‑238 (MQ=255)                                                                                tgcgGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGCGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGAG‑ACCCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGCTCTCCATCACTTTCATGGAACCGAACTTCCCCCCTTGG‑CTCGGCC‑TGCCACCTATCCCGGAGCAGAACTGGCGCAAAAAAACCGGcccgcggta                                                                                                                                                           >  1:495486/1‑233 (MQ=255)                                                                                tgcgGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGCACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCCGGTGa                                                                                                                                                           >  2:39162/1‑239 (MQ=255)                                                                                tgcgGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGATGCTGGCGGAAAATAACCGGCCCTGGTGa                                                                                                                                                           >  2:3659/1‑239 (MQ=255)                                                                                tgcgGACGTCGACCGACAATCTACATCCCCCCGGT‑CAGGACATTGATAAACTGCGGCA‑ATTCATCCAGCTAGGTAGG‑GCCCAGAAAGGTCACCAC‑TTT‑GGTG‑CCGCGCGC‑ATC‑GTTCTGCTTCGCCTGGGTCCCCCCTTTGAGTGTCTCCACCAATTCAATGGAAAGGAACTTCCACACTCTG‑ACCGTCG‑TGCCCTCACTCCCGTACCAAGTCTGGCGGAAAACAAACAGCCCCgggag                                                                                                                                                           >  1:886124/1‑236 (MQ=255)                                                                                tgcgGACGAGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTAACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACCCTTTA‑ATAGGCT‑TGCCATCCCTCCCGTAGCGAGACCGGCGTAAAATAACCGGcctggcgga                                                                                                                                                           >  1:1064850/1‑232 (MQ=255)                                                                                     aCGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCAATTCCTGAGCAGGTCTGTCGGAAAATAAACGGccccgtgaaaagaa                                                                                                                                                      >  1:341365/1‑228 (MQ=255)                                                                                          gACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ACCGGCT‑TGCCATCCATCCCGGAGCGAGGCTGCCGGAAAAAAAACGGCCCGGGGGAAcgggggtt                                                                                                                                                  >  1:508320/1‑231 (MQ=255)                                                                                             cGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGCCT‑TGCCATCCATGCCCTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGGGAACTGGGttttcc                                                                                                                                               >  2:1221743/1‑235 (MQ=255)                                                                                               gcAATCGACATCCCCCCGGT‑CAGCACATTGATACACTGCGGCA‑ATTCATCCAGCGAGGCACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TACCATCCATCCCGTAGCGCGGCTGGCGGAAAAAAACACGCCCCAAGGAACAGAGATTCACCGc                                                                                                                                            >  1:76329/2‑239 (MQ=255)                                                                                               actcttcccATCCCCCCTGT‑AACCACTTTGCTAAAC‑GCGGCATCTTCCCCCGGGGGGGTCCG‑TGGCATAAGGTCCCCGCT‑TTT‑GGTG‑CCGCTGGG‑TTC‑GTCTGGGGTCGCCTGGGTCCCCACTTTGTCTGTCCCCACCACTTTCATGGAACGGACCTTCCTCTCTTTG‑AGCGGCT‑GGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGc                                                                                                                                            <  1:77600/231‑1 (MQ=255)                                                                                               acactccccttCCCCCCGGG‑AAGCACATTGAAAACCTGCGGCA‑AGTCACCCGGCGGGGTCCG‑GCG‑AAAAAGTTCCCCCCTTTT‑GGTG‑ACGCGGGG‑ATC‑GTTCGGGGTCGCTGGGTTCCCCACTTTGTCGTTCTCCAACCTTTCCATGGACCGGAATTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGAAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGc                                                                                                                                            <  1:483506/229‑1 (MQ=255)                                                                                               aCAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCg                                                                                                                                             >  2:941718/1‑238 (MQ=255)                                                                                               aCAATCGACACCCCCCCGGT‑CAGCACATTGATAACCTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGc                                                                                                                                            <  2:1067329/239‑1 (MQ=255)                                                                                               aCAACCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTG‑GGTG‑ACGGGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACCCTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGc                                                                                                                                            <  2:118579/239‑1 (MQ=255)                                                                                                acaaccagccccccccGGTGCAGCTCATTTATAACGTGGGGTG‑TTTCATCGAGGGAGGCGCG‑GTGCACAATGTTCCCCCC‑TTT‑GGTG‑ACGC‑CGG‑ATCGGTTCTGCGTCGCCTGGGTCAGCTCTTTGTCCTTCCCCAACACTTGCAGGGAACGGACCTTCCAACCTTTA‑ATCGGGT‑TGCCATCCATGCCGTAGCGAGTCTGGCGAAAAAGATCGGCCCCTGGGGAACTGAGTTTAACCGc                                                                                                                                            <  1:39162/229‑1 (MQ=255)                                                                                                    cgccccccccccGGT‑CCGCACATGGACCACCTGCGCCA‑ATTCACCCACCG‑GGGACGCGGGCAGAAA‑TCCCCCCC‑TTT‑GGGGTACGCGCGG‑CTC‑GTTCTGCGGCCGCTGGGTCCCCCCTGTGTCGTTCTCCATAACTTTCAGGAAACGGAACTTCCAAAGGGGA‑ATGGGCT‑TTACATCCAGTCCGTGGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTAACCGCCAgcg                                                                                                                                       <  1:1272435/233‑1 (MQ=255)                                                                                                         tCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGGGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑a                                                                                                                                 <  2:1026724/239‑1 (MQ=255)                                                                                                                 gT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑acagcaca                                                                                                                          >  2:100631/1‑238 (MQ=255)                                                                                                                  t‑CAGCCAATTGAAAAACTGCGGCA‑ATTCACCCAGCGAGGTACG‑GGCCAGAAAGTTCCCCAC‑TTT‑GGGG‑ACGCGGGG‑ATC‑TTTCTGCGTCGCCTGGGTCCCCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑acagcacag                                                                                                                         <  2:1324555/238‑1 (MQ=255)                                                                                                                  t‑CAGCACTTTGAAAACCTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCCCTTTCATGGAACGGAACTTCCACATTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑acagcacag                                                                                                                         <  2:290226/238‑1 (MQ=255)                                                                                                                       cccaTTGATAACCTGCGGAA‑GTCCCCCCCGCGGGCTCGG‑CCGGAGAAA‑TTCCCCAC‑TTTGGGTG‑ACG‑GCGGCATG‑TTTCGGCGTCGCCTGGGTCCCCTCTTTGTCTGTCCCCAACCTTTTCAGGGAACGAACTTTCCACATTTGG‑ACCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGAAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCg                                                                                                                    <  1:395195/237‑1 (MQ=255)                                                                                                                       cacaTTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCCCACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCCCCg                                                                                                                    >  2:736930/1‑239 (MQ=255)                                                                                                                         caTTGAAAAACTGCGGAC‑ATTCATCCGGGGGGGTACG‑GGCAAGAAAGTCCCCCCC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCCCCACTTTGTCGTTCTCCATCCCTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCggg                                                                                                                  <  2:431954/239‑1 (MQ=255)                                                                                                                          aTTGATAAACTGCGGCA‑ATTCATCCGGCGAGGTACG‑GCGAAGAAATTTCCCCAC‑TTT‑GGTG‑ACGGGGGG‑ATC‑GTTCTGCGTCGCCGGGGCCACCACTTTGTCGTTCTCCATCCCTTTCAGGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCgggg                                                                                                                 <  2:341365/239‑1 (MQ=255)                                                                                                                            tGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCCCTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGc                                                                                                                                                                                          <  2:664905/165‑1 (MQ=255)                                                                                                                            tGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCCCAGCACCgggggg                                                                                                               >  2:756638/1‑237 (MQ=255)                                                                                                                            tGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GATCTGCGTCGCCTGGGTCACCACTTTGGCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTATCGAGGCTGGCGGAACATACACGGCCCTGGTGAACGGACTTTCACCGCCACGGCAAA‑ACAACAAAGCACCCGGgaa                                                                                                               >  1:266947/1‑238 (MQ=255)                                                                                                                            tGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑ATTATGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGATTCTGGCGGAAAATACACGGCCCTGCTGAACTGAGTTTCAACGCCA‑CGGAATAAAAGCCCAGCACCGGGgaa                                                                                                               >  1:503344/1‑238 (MQ=255)                                                                                                                                 aaCTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGccat                                                                                                                                                                                                      >  2:1278033/1‑148 (MQ=255)                                                                                                                                 aaCTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGGGATca                                                                                                           >  2:1110873/1‑238 (MQ=255)                                                                                                                                 aaCTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATca                                                                                                           >  2:654329/1‑238 (MQ=255)                                                                                                                                 aaCTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTACCGAGTCTTGCGGAAAATAACAGGCCCTGGTGCACTGGGTTTCCCCCCCCCCCCACT‑acagaaaaacccccgggggaccaa                                                                                                          >  1:1161219/1‑219 (MQ=255)                                                                                                                                    tccGGCA‑TTTCACCCAGCGAGGTCGG‑GGCCAAAAATTTCCCCAG‑TTT‑GGGG‑GCCCGCGG‑TTG‑TTTCGGGCGCGCCGGGTACACCCCTTTGTCGTTCCCCCTCATTTTCAGGGAGCGGACCTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTGGCGAGTCTGTCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATcagcag                                                                                                       <  1:941718/237‑1 (MQ=255)                                                                                                                                      cGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGTAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGCGATcagcagca                                                                                                     >  2:817706/1‑239 (MQ=255)                                                                                                                                             ttCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGATCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCCTCCATGCCGTAGCGAGTCTGGCGGCAAATAACCGGCCCTGGTGAACTGAGGTTCCCCCCCAGCGCCAT‑ACAGCAAAGCACCGGGGAGATCAAACGCAGAaaaa                                                                                               >  1:477123/1‑239 (MQ=255)                                                                                                                                                    ccggtggTGCG‑GCGCTGTAATTCCCCCCC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTTTGGGTCCCCTGGGCCCCCCCTTTGTCGTTCCCCATCCCTTTCATGGACCGGACCTTCCACCCTTTG‑ATCGTCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGAAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGc                                                                                        <  1:817706/234‑1 (MQ=255)                                                                                                                                                      cGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑acagcacagc                                                                                                                        >  2:3118/1‑205 (MQ=255)                                                                                                                                                       taGGTACG‑GCGCAGAAATTTCCCCCC‑TTT‑GGTG‑ACGCGTGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCCCTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACAGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGc                                                                                        <  2:1317995/235‑1 (MQ=255)                                                                                                                                                         ggtggg‑gCGCAG‑AAGTTCCCCCG‑TTTGGGTG‑CCGCGCGG‑ATC‑GTTCTGGGTCGCCTGGGGCACCATTTTGCCGTTCTCCATCACTTTCATGGAACGGACCTTCCACCCTTTG‑CTCGCCT‑TGCCATCCATGCCGTAGCGAGTTGGGCGGAAAATACCCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGc                                                                                    <  1:3659/233‑1 (MQ=255)                                                                                                                                                             agggccaaGAAAGTTCCCCTG‑TTG‑GGTG‑ACGCGCGG‑ACC‑TTTCTGCGGCCCCTGGGTACCCACTTGGCCGTTCTCCATCACTTTCAGGGAACGGAACTTCCACCCTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGcaca                                                                                 <  2:1007850/231‑1 (MQ=255)                                                                                                                                                                                    tt‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGTTCCCCATTTTGTCGTTCCCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAg                                                          <  2:1076047/239‑1 (MQ=255)                                                                                                                                                                                     t‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCCACGCCAGCACAATGCCTTCCGccggcttgcgccgg                                                         >  2:514285/1‑226 (MQ=255)                                                                                                                                                                                        gTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGGCGCCAGCCCAATGTCTTCCGCCGGTTTGTGCAGGCg                                                       >  2:348653/1‑239 (MQ=255)                                                                                                                                                                                               cgcgG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCCCACTTTG‑ATCGGCT‑TGCCATCCATGCCGTATCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCCCAGCCCCGGGGAGATCAGCAGCAGCATAAGCGTCGCCACCCCACATTCATCCGCACGATTGAgagacgggctaac                                                 >  1:475204/1‑227 (MQ=255)                                                                                                                                                                                               cgcgG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCCGAATAAGCGTCGCCAGCACAAATTCTTCCCCCCGTTTGAGCAGGCGGGTaa                                                  >  1:1076047/1‑238 (MQ=255)                                                                                                                                                                                               cgcgG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGACCAGCCCCGGGGGGGTCAGCAGCAGAATAAGCTTCGCCAACCCAAAGGCATCCGCAAGGTGGaggaggagggaaa                                                  >  1:539129/1‑234 (MQ=255)                                                                                                                                                                                                              gCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGGGCAGGCGGTTAA‑CCCCGGAAAgcggc                                   >  2:715055/1‑239 (MQ=255)                                                                                                                                                                                                               cGTCGCCTGGGTCACCCTTTTGTCGTTCTCCATCCCTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAA‑CCCCGGAAAgcggc                                   <  2:957339/238‑1 (MQ=255)                                                                                                                                                                                                                    ccTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCGCGGTTGAGCGGGGGGTTAA‑ACCCCGAAAgcgggggggag                             >  1:908358/1‑232 (MQ=255)                                                                                                                                                                                                                    ccTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAA‑CCCCGGGAAGAGGCGTGTCg                             >  2:309656/1‑239 (MQ=255)                                                                                                                                                                                                                            ccccttttGTCGTTCCCCATCACTTTCATGGAACGGAACTCCCACACTTTG‑ATCGGCT‑TGCCATCCAGGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAA‑CCCCGGAAAGCGGCGTGTCGTAAAGCgg                     <  2:495486/234‑1 (MQ=255)                                                                                                                                                                                                                                  ttGTGGTTCCACATCACTTACATGGAACGGAATTTCCACCCTTGA‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAA‑CCCCGGAAAGCGGCGTGTCGTAAAGCGGcaccac               <  2:886124/239‑1 (MQ=255)                                                                                                                                                                                                                                       gTTCTCCCACACTTTCAGGGAACGGAACGTCC‑CCCTTTGAACCGGCT‑TGCCATCCTTGCCGTAGCGGGTCTGGCGGAAAATAACCGGCCCTGTGGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAA‑CCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTa          <  2:13201/239‑1 (MQ=255)                                                                                                                                                                                                                                        ttCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAACCGTCGCCAGCCCACAGCCTTCCGCACGTTTTAGCAGGCGGGTAACCCCCGAAAAGCGCGGTGTGGAAAAGGGGccccccggga           >  1:214964/1‑232 (MQ=255)                                                                                                                                                                                                                                               tCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAA‑CCCCGGCAAGCGGCGTGTCTTAAAACGGCACCACCGGTAGGCCGTTc  >  2:819423/1‑239 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 GGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGTTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGT‑CAGCACATTGATAAACTGCGGCA‑ATTCATCCAGCGAGGTACG‑GCGCAGAAAGTTCCCCAC‑TTT‑GGTG‑ACGCGCGG‑ATC‑GTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTG‑ATCGGCT‑TGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAAT‑ACAGCACAGCACCGGGGAGATCAGCAGCAGAATAAGCGTCGCCAGCACAATGTCTTCCGCACGTTTGAGCAGGCGGTTAA‑CCCCGGAAAGCGGCGTGTCGTAAAGCGGCACCACCGGTACGCCGTTC  >  NC_000913/2120328‑2120796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑