Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,459,223	T→C	25.4%	G415G (GGA→GGG)	fadI ←	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,459,223	0	T	C	25.4%	128.5 / 9.2	85	G415G (GGA→GGG)	fadI	beta‑ketoacyl‑CoA thiolase, anaerobic, subunit
Reads supporting (aligned to +/- strand):  ref base T (41/16);  new base C (2/21);  total (48/37)
Fisher's exact test for biased strand distribution p-value = 1.90e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAG  >  NC_000913/2458995‑2459459                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 cGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCAcccccg                                                                                                                                                                                                                                         >  2:145231/1‑238 (MQ=255)       gCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACCAAACC‑AAA‑TCCCCCCCCGCCC‑CGGc                                                                                                                                                                                                                                 >  2:1081276/1‑239 (MQ=255)         ccttctTTTTTTTCACGGTGTTTGTTATATATTGCGCCCACCTGCGGGGCAATCCACCCCTTAAGGGTTTTCATTTCCCCCCCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGACCATTAAGGGTAAACG‑CTGATGCCATTTCCATT‑ATTCCGCCACCAGAA‑CCATGGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAACCC‑AAA‑TCCACCGCCGCGA‑CGGCGa                                                                                                                                                                                                                               <  1:690489/236‑1 (MQ=255)           ctctTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGAGCCAAGCCCACCGGCAGCACAGGCGGAAACTAAAAC‑AAA‑ACCACCCCCCCGA‑CGGCGAAg                                                                                                                                                                                                                             >  1:408064/1‑239 (MQ=255)              tttGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCCAAGGCGGAAACTAAACA‑AAA‑ACCAcccccggaaccggcgagttt                                                                                                                                                                                                                           >  2:788191/1‑225 (MQ=255)                tGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAACCCCCCGGCAGAACAGGCGGCACATAAAAC‑AAA‑TCCACCCCCGCCACCGGCG‑AGTTC‑ac                                                                                                                                                                                                                       >  1:925794/1‑238 (MQ=255)                  ttttgcggggttttcTTAATTGTGGCGCAGCCCGGCCAGGCAAACCCCCACTGCGGTGTTTTCATTTGCTCCCCCGGTAGGCCGATGGTTTGAACGGCAATGTTTTCCAG‑ACTACCATTAAGGTTAAACG‑CTGATGTCATTGCCATG‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACGGGCAGCACAGGCGGAAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGc                                                                                                                                                                                                                     <  1:979306/225‑1 (MQ=255)                     tCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCCCCAAGCCCACCGGCAGCACAGGCGGTAACCAAACA‑CAA‑ACCCCCCCCCCGA‑CGCCCAAGGTC‑CAGCaaa                                                                                                                                                                                                                  >  1:1170456/1‑238 (MQ=255)                      cACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAACCCCCCGGCCGCACAGGCGGGAAACAAACC‑AAA‑CACACCCCCCCGC‑CGGCGAAGTTA‑ATGCaaag                                                                                                                                                                                                                 >  1:723094/1‑237 (MQ=255)                       cgggattgtgttAAAAAGGGCGCGCCCCGGCGAGGCAACCCCCGCCTTCGGGTTATTCATTTTCCCCCCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGt                                                                                                                                                                                                                <  2:203167/229‑1 (MQ=255)                            tttGGTTAATAAGGGCGCGCCCCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTgg                                                                                                                                                                                                            <  2:367819/237‑1 (MQ=255)                            gTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAAACAAAAC‑AAA‑CCCCCCCCCCCGC‑CCGCCAAAGTC‑AAGCaaattcaggg                                                                                                                                                                                                           >  1:1241311/1‑231 (MQ=255)                             ttGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCCCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑t                                                                                                                                                                                                         >  2:1011320/1‑239 (MQ=255)                                     taatGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCCCGC‑CGGCCAAGTCA‑ATCCAAAGGCTGGG‑TAatcatc                                                                                                                                                                                                  >  2:49478/1‑238 (MQ=255)                                       atGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGACGTTC‑CTGCAACGTCTGGG‑TAATCATcctc                                                                                                                                                                                               >  2:393055/1‑237 (MQ=255)                                          gcgcgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑CAA‑ACCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCcgcgc                                                                                                                                                                                             >  2:508672/1‑238 (MQ=255)                                          gcgcgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCCCCGCCCCGA‑CGGCGAAAGTC‑ATGCAAAGTCTGGG‑GAatcactgcccct                                                                                                                                                                                            >  2:1234658/1‑231 (MQ=255)                                          gcgcgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCCGGG‑GAATCATcccccc                                                                                                                                                                                             >  2:916769/1‑234 (MQ=255)                                          gcgcgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCCCCGCGA‑CGGCCAAGTTC‑ATGCAATGTCTGGG‑GAATCATCcgccc                                                                                                                                                                                             >  2:1234906/1‑236 (MQ=255)                                             cgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCg                                                                                                                                                                                         >  2:1191024/1‑239 (MQ=255)                                             cgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCCCCCCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑GAATCATcccgccgccg                                                                                                                                                                                         >  2:58263/1‑231 (MQ=255)                                             cgcACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCCCCCCGA‑CGGCCAAGTTC‑ATTCAATTCCTGGG‑CAAACATCCTCGccccc                                                                                                                                                                                         >  2:1032586/1‑238 (MQ=255)                                                cccggggAGGAAACCTCCCCCTTCAGGCTATTAGTTTTCCCACCCGGTACGTGGATGGTAAAACCGTCAAGGTTGTCCAG‑AAGAAAATTAAGGTCAAAGG‑CGAAGGCCTTATCCATT‑ATCCCGCCTCCAGAA‑CCATGGCCGCCCCAACCCCACAGGCAGCACAGGCGGAAACTAAACC‑AAA‑CCCTCCGCCGCGA‑CGGAGAAGGTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTc                                                                                                                                                                                      <  1:1311082/233‑1 (MQ=255)                                                            cttcccgcttttcggtgttttaTTTTCTCACCCGGTACGTCGTTGTTGATAACCGCTAGTACGACCGC‑GCTAAAATTAAGGG‑AAACGTCGGATGGCATTTCCATT‑ACTCCGCCCCCACAA‑CCATCGCCGCCCCAGGCCCCCCGGCAGCAGAGGCGTTATCCAAACG‑AAA‑CCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCACCCGCGCGCCGGTCGCCGCAAAGGGa                                                                                                                                                                          <  1:1079242/218‑1 (MQ=255)                                                            acctcctctttggggttttttaTTTTCCCCCCCGGTAGGTCGAGGGTGAAACCGGAAAGGTTGCCCAA‑CCGAACATAAAGGGTAAACG‑CTGCGTTCATTCCCATT‑ATCCCGCCACCAAAC‑CCATTGCCGCGCCAAGCCCACCGGCGGCCGAGGCGGTAACAAATCC‑AAA‑CCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGa                                                                                                                                                                          <  1:344941/218‑1 (MQ=255)                                                            acccccccttacgggttATTCATTTCCTCACCCGGTCCGTGGAGGGTGATAACGGAAATGTTGCCCAA‑ACAAACATAAAGGGTAAACG‑CTTATGCCATCTCCATT‑ATCCCGCCCCCAGAA‑CCATTCCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑CCCCCCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGa                                                                                                                                                                          <  1:1191024/223‑1 (MQ=255)                                                            aaCTCCGCCTTCGTGGTTTTATTTTTCCAACCGTGGACGTCGATGGTGCAACCGGAAATGTTGCCAAG‑GCGAACATTAAGGGTAAACG‑CTGATGCCATTTCCATT‑ATCCCGCCTCAAAAA‑CCATTCCCGCGCCAAGCCCACGGGCAGCACAGGGGGTAACTAACCC‑AAA‑CCCCCCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGa                                                                                                                                                                          <  1:145231/239‑1 (MQ=255)                                                            aaCTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGGAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCCATGTCTGGG‑TAATCATCCGCGCGCCGGCCCCCGCGAGGGGa                                                                                                                                                                          >  2:1079242/1‑239 (MQ=255)                                                                    cTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCCCCGACA‑CGGCGAAGTTC‑ATGCAATGTCTTGG‑TAATCCTCCGCGCGCCGGTGGCCGCGAAGGGAAGGCCggg                                                                                                                                                                  >  2:136438/1‑237 (MQ=255)                                                                              ttatttttCCCCCCCGGTAGGTGGAGGGTGATAACGGAAATGTTGCCCAA‑ACAAACATAAAGGGTAAACG‑CTGATTTCATTCCCATT‑TTCCCGCTCCCAAAC‑CCATCGCCGCGCCAGGCCCCCCGGCAGCACAGGGGGTAACTAAACC‑AAA‑CCCCCCGCCGCGA‑CGGCGAAGTTC‑ATGCAAGGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGaa                                                                                                                                                        <  1:1234906/235‑1 (MQ=255)                                                                              ttCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGTCGGTCACTAAACC‑AAA‑TCCAACCCCCCGA‑CGGCGAGCATC‑ATGCAACGTCCGGG‑GAATCATCCCCGACGCGGGCGCCCCGAAggggtccacctcaggaaaaca                                                                                                                                                         >  1:1271102/1‑220 (MQ=255)                                                                              ttCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑CAA‑TCCCCCGCCCCGC‑CCGCGAAGTTC‑ATGCAATGTCTGGG‑GAAACAACCACGCACCGGGCCCCCCGCAGGGATGCCCGAAAGCAAACGaa                                                                                                                                                        >  1:359196/1‑239 (MQ=255)                                                                              ttCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCCATTCCTGGG‑TAATCATCCGCCCCCCGGGCGCCCCGCAGGGGTGCCCGTAAGCAATCGa                                                                                                                                                         >  2:622849/1‑238 (MQ=255)                                                                              ttCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCCGGG‑TAATCATCCGCCCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGa                                                                                                                                                         >  2:906123/1‑238 (MQ=255)                                                                                      ctcACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑GAATAATCCGCGCGCCGGTCGCCCCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAg                                                                                                                                                >  2:611350/1‑239 (MQ=255)                                                                                               tACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGCCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CCGCGCAGTTC‑ATGCAATGGCTGGG‑GAATCATCCGCCCCCCGGTGGCCCGGAAGGGATGCCCCGAAGCAATCAAAACCCCacacagcgaaaa                                                                                                                                       >  1:469418/1‑228 (MQ=255)                                                                                                     cgatgtgtaaaatggaaTTTTGTCCCGAACGAACATTAAGGG‑AAACG‑CCAGCTTCCTTTCCCTTGACCCCCCCGCCAAAA‑CCATGCCCCCCCCAAGCCCCCAGCCAGCAAGGGGGGAAACTAACCA‑AAA‑CCCCCCGCCGGGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTg                                                                                                                                  <  1:622849/223‑1 (MQ=255)                                                                                                         gTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑CAA‑ACCCCCGCCCCCC‑CGGCCAAGTTCAATCCAATG‑CTGGG‑TCAACATCCCCCCGCCCGTCGCCGACAAGGGGAGGCCGTTGACAATCGGACCCCCAAGCCCGTT‑AAAATTGCTAtag                                                                                                                            >  1:56082/1‑237 (MQ=255)                                                                                                         gTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAAATTGCTATc                                                                                                                             >  2:316049/1‑238 (MQ=255)                                                                                                            aTAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGGTC‑ATGCAATGTCTGGG‑GAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCCCCAAGCACGGT‑AAATTTGCCATCGTcc                                                                                                                         >  2:635899/1‑239 (MQ=255)                                                                                                            aTAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAAATAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATCCAATGTCTGGG‑CAATCACACGCGCGCCGGGCGCCCCGAAGGGACTCCCGTAAGCAATCAAACACCCaacacagctaaaatgcgtatcgcc                                                                                                                           >  1:883871/1‑216 (MQ=255)                                                                                                                 ggCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCCCCCCGA‑CCGCGCAGTTC‑ATGCAATGTCTGGG‑TAATCACACCCGCGCCGGCCCCCCCGAAGGGGTGCCCCGAACGAATCCACCCCCCCACCACCTTAAAATTTGCAAtgcccccct                                                                                                                      >  1:1113788/1‑230 (MQ=255)                                                                                                                   cAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACCAAACC‑AAA‑TCCACCGCCGCCA‑CGGCGAAGATC‑ATGCAAAGTCTGGG‑TAATCATCCCCCCGCCGGGCACCCCCAAGGGATGCCCCGAAGCAACTCAACCCCCCAACACCTT‑AACATTGcctactgccccatca                                                                                                                   >  1:85256/1‑224 (MQ=255)                                                                                                                     attttggcaag‑aagaACATTAAGGGTAACCG‑CTAATGTCATTCCCATT‑ATCCCCCCTCCAGAA‑CCATGGCCGCGCCAAGCCCCCCGGCAGCACAGGGGGAAACTAACCC‑AAA‑TCCCCCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCa                                                                                                                <  2:1193263/230‑1 (MQ=255)                                                                                                                        ttgtCCAG‑ACGAACATTAAGGGTAACCG‑CTGATGTCATTTCCATT‑ATCCCGCCTCCAGAA‑CCATTGCCGCGCCAACCCCACCGGCAGCACGGGCGGTAACTAACCC‑AAA‑CCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGtt                                                                                                                                          <  2:1149763/211‑1 (MQ=255)                                                                                                                           tCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑CAATTTGCTATCGTTCCCTTCGCCAGTGGCa                                                                                                          >  2:198879/1‑239 (MQ=255)                                                                                                                           cccAG‑ACGAACATTAGGGGTAAACG‑GTGATGCTATTTCCATT‑ATTCCGCCCCCAGAC‑CCATTGCCGGGCCAAGCCCCCCGGCAGCACAGGCGGTAACTAAACA‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCa                                                                                                          <  2:1113788/238‑1 (MQ=255)                                                                                                                                gcgtaaCATTAAGGGTAAGCG‑CTGATGTCATTCCAATT‑ATTCCGCCTCCAAAA‑CCATTGCCGCCCAAAGCCCACCGGCAGCCCAGGCGGTAACTAACCC‑AAA‑CCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCAtgtg                                                                                                      <  2:925794/235‑1 (MQ=255)                                                                                                                                  ccacatttAAGGTAAAACG‑CTGATGTCATTCCCATT‑ATCCCGCCCCCAGAA‑CCATGGGCGCCCCAAGCCCCCCGGCAGCACGGGCGGTAAATAAACC‑AAA‑TCCCCCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCa                                                                                                    <  2:469418/233‑1 (MQ=255)                                                                                                                                      cATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAACCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCCCCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGCAGGGAAGCCCGTAAGCAATACAACCCCCCACCACGTT‑CACTTTGCTATCCTCCACCTCCCCAGAGACATGTGccccc                                                                                                 >  1:126085/1‑234 (MQ=255)                                                                                                                                            gggTAACCG‑CTGATGTCATTCCCATT‑ATCCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCCCCGGCAGCAAAGGCGGTAACTAAACC‑AAA‑TCCCCCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACg                                                                                                  <  2:545805/231‑1 (MQ=255)                                                                                                                                             ggTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑GAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCCCCAGTGCCCTTGGCCCGcccccgg                                                                                           >  2:1217151/1‑234 (MQ=255)                                                                                                                                                 aaCG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAAATTGCTATCGTCCATTTCCCCAGTGGCATTGGCACCCCCCCGTGGTTc                                                                                      >  2:818134/1‑238 (MQ=255)                                                                                                                                                 aaCG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGGTCCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCACGCCCGTT‑AAATTTTCTCTCCTCCCCTTCCCCAGGGGCATGTGAACCCCCCAACGCTTc                                                                                      >  1:1263787/1‑238 (MQ=255)                                                                                                                                                       tGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCCGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCCGAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCCATCGGCCCCTTCGCCAGTGGCATGTGGAcccacccgagggtttgggg                                                                                 >  1:545805/1‑220 (MQ=255)                                                                                                                                                        gATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCCCGCCCCACTGGTTCCCGCGCa                                                                               >  2:1050240/1‑239 (MQ=255)                                                                                                                                                        gATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGAATGTGGACGCCCCCGAGCTTCAcacgcc                                                                               >  2:14074/1‑234 (MQ=255)                                                                                                                                                        gATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAAACGCCAAGCACGTT‑AAATTTGCTATCGTCCCATTCGCCAGTGGCCTTTGCACGCCCCAAGGCTTCCCGAGcc                                                                               >  2:313803/1‑238 (MQ=255)                                                                                                                                                              aTTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCATTGGCATGGGCAAGCCCCCGGGCTTCAccggacaaac                                                                           >  2:190322/1‑228 (MQ=255)                                                                                                                                                              aTTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGGTCACGCCCCAGTGCCTCACGAGCCAAACGt                                                                         >  2:1066584/1‑239 (MQ=255)                                                                                                                                                               tttCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCCCCAAGCCCCCCGGCAGCACGGGCGTTAACTAAACC‑AAA‑TCCCCCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGtt                                                                        <  2:723094/239‑1 (MQ=255)                                                                                                                                                                     ttaatcccccccccAGAA‑CCTTCGCCGCGCCACGCCCACCGGCAGCCTAGGCGGTACCTAACCC‑ACA‑CCCCCCCCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTa                                                                   <  1:1255982/227‑1 (MQ=255)                                                                                                                                                                     attagcccGCCCCCAGAC‑CCATTGCCGCGCCAACCCCGCCGGCAGCACGGGCGTAAACAAAACC‑AAA‑CCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTa                                                                   <  2:126085/233‑1 (MQ=255)                                                                                                                                                                        tttCCCCCCCCACAACCCATCGCCGCCCCGACCCCCCCGGCCGCCCAGG‑GGTAAAAAAACC‑AAACCCCCCCGCGGGGA‑CGGCGAAGTTC‑ATGCAATGTGTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTAcc                                                                 <  1:1011320/238‑1 (MQ=255)                                                                                                                                                                              cccccAAAA‑CCATTGCCGGCCCAAGCCCCCCGGCAGCACGGGCGTTAACAAAACC‑AAA‑CCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAAc                                                          <  2:1170456/236‑1 (MQ=255)                                                                                                                                                                              ccTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACt                                                                                                                      >  2:728825/1‑179 (MQ=255)                                                                                                                                                                                           tgcccGCCCCAAGCCCCCCGGCAGCCGAGGTGTTAACAAACCC‑AAC‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGcc                                              <  2:1164112/236‑1 (MQ=255)                                                                                                                                                                                                   ccagccccACCGGCAGCACAGGGGGTACCAAAACC‑AAA‑CCCGCCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTg                                      <  2:343340/234‑1 (MQ=255)                                                                                                                                                                                                    caccccccccGGCTACACGGGGGATAACACAACC‑AAC‑CCCCCCGCCGCGG‑CGGCGAAGTTC‑CTGCAATGCTTGGG‑TAACCCACGCCGCGCGGGTCGCCACGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGa                                     <  1:611350/235‑1 (MQ=255)                                                                                                                                                                                                         ccACCGGCAGCACGGGCGTTACCAAACCC‑AAC‑TCCCCCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGc                                   <  2:359196/236‑1 (MQ=255)                                                                                                                                                                                                            cgctcagacacagtgggtaCTAAACCAAAA‑CCCGCCGCCGCAA‑CGCCGAAGGTC‑ATG‑AACGTCCGGGTTAACTCTCCGCGCCCCGGTCGCCGCAAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCaa                              <  1:151303/221‑1 (MQ=255)                                                                                                                                                                                                            ccGGCAACAAGGGCGGAAACTAACCC‑AAA‑CCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCaaa                             <  2:1263787/239‑1 (MQ=255)                                                                                                                                                                                                                cagccaaGGCGGTAACTAACCC‑AAC‑TCCCCCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGc                          <  2:1271102/232‑1 (MQ=255)                                                                                                                                                                                                                caccacgGGCGGAAACTAAACC‑AAA‑TCCCCCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGc                          <  2:342092/235‑1 (MQ=255)                                                                                                                                                                                                                           tgtaCTACACC‑AAC‑CCCCCCCCCGCGG‑GGGCGAAGTGC‑ACGCATGGTCTGGA‑TAACCCTCCGCGCGCGGGTCGACGTGAAGGGATGCCCTTAAGAGAGCGCACCGCCAAGCACGTT‑AAATTTGCTATCGACCACTTCGCCAGGGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCatat               <  1:704015/235‑1 (MQ=255)                                                                                                                                                                                                                           gTAACTAAACC‑AAA‑TCCCCCGCCGCGA‑CGGCGAAGTTA‑ACGCAATGTCTGGG‑AAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCatat               <  2:79202/238‑1 (MQ=255)                                                                                                                                                                                                                             aaCTAACCC‑AAA‑TCCCCCGCCGGGC‑AGCAGAAGTTC‑ATGCAAGGCCTGGG‑TAAACATCCGCGCGCCGGCCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCATTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCg             <  2:613212/238‑1 (MQ=255)                                                                                                                                                                                                                              acaaaccc‑AAA‑CCCACCGCCGGGA‑CGGCAAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt           <  2:817890/233‑1 (MQ=255)                                                                                                                                                                                                                              acaaaacc‑aaa‑ccCCCCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGCCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt           <  2:490493/236‑1 (MQ=255)                                                                                                                                                                                                                              aacacaCC‑ACA‑CCCCCCCCCGCGG‑AGGGGAAGTTC‑ATGCCATGGCTGGG‑TAATCCTCCGCGGGCCGGTCGCCGGGAAGGGATGCCCGTAACCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt           <  1:1069821/234‑1 (MQ=255)                                                                                                                                                                                                                               ccaaacc‑aaaccCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt           <  2:722406/237‑1 (MQ=255)                                                                                                                                                                                                                               acaaaCCAAAA‑CCCCCCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGAt           <  2:1241311/237‑1 (MQ=255)                                                                                                                                                                                                                                  aaCC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGa                                                                                                                                                         >  2:1021423/1‑94 (MQ=255)                                                                                                                                                                                                                                  aaCC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGa                                                                                                                                                         <  1:1021423/94‑1 (MQ=255)                                                                                                                                                                                                                                  aaCC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCACCGCCTGAGCTGCAAAAGCTTCGTGCATATCGATCAAt       >  2:807608/1‑239 (MQ=255)                                                                                                                                                                                                                                  aaCC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCCATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCACAACCTTCACCACCCACCAACTCAAACTTCCCCGGCGCCCGAACTCCAAAGACCTCGGGCAAATcaaaaaaa       >  1:631572/1‑232 (MQ=255)                                                                                                                                                                                                                                        aa‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACCCCCCAGTGCTTCACGGGCAAAACGGTCACTACCCAGCAACTGAATATTCGCCCGCGTCTGAGCGGCAAAGGCTTCGTGCCTATCGATCAATTTCAg  >  2:1069294/1‑239 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 CGCCTCGCAACTCTTTGTTTTCACGGAGTTGCTTAATAATGGCGCGCACCTGCGAGGCAAACTCCGCCTTCAGGGTATTCATTTTCTCACCCGGTACGTCGATGGTGATAACGGCAATGTTGTCCAG‑ACGAACATTAAGGGTAAACG‑CTGATGTCATTTCCATT‑ATTCCGCCTCCAGAA‑CCATTGCCGCGCCAAGCCCACCGGCAGCACAGGCGGTAACTAAACC‑AAA‑TCCACCGCCGCGA‑CGGCGAAGTTC‑ATGCAATGTCTGGG‑TAATCATCCGCGCGCCGGTCGCCGCGAAGGGATGCCCGTAAGCAATCGAACCGCCAAGCACGTT‑AAATTTGCTATCGTCCACTTCGCCAGTGGCATGTGCACGCCCCAGTGCTTCACGAGCAAAACGTTCACTACCCAGCAACTGAATATTCGCCAGCGTCTGAGCTGCAAAGGCTTCGTGCATATCGATCAATGTCAG  >  NC_000913/2458995‑2459459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑