Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258675 258683 9–776 79 [0] [1] 79 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)72 (1.230) 53/402 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers

CCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCG  >  NC_000913/258469‑258906
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cccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgagggaaAGGGCAAAGTTAAt                                                                                                                                                                                                                    >  1:30605‑M2/216‑229 (MQ=255)
        acccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaaaagcagattgatcaaaaaatttaccgcacagggcgcgtataatcgagaaggaaAGTGAAAAAAAAAACAATTCtt                                                                                                                                                                                                            >  2:306142‑M2/208‑229 (MQ=255)
         cccggtgtatcggaattttgacaaaggtctacaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggcgatagcaatgacgttaccgagtggacaccagaagaccagattgatcaaaaaaattacagcactaggcccgtatatccgtgtaggaaAGTGCAAAATAAATCGAATCttt                                                                                                                                                                                                           >  2:19405‑M2/207‑229 (MQ=255)
             gtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaagggaAGTGCAAAGATAATCGATTCttttt                                                                                                                                                                                                         >  1:441482‑M2/203‑227 (MQ=255)
              tgtatcggaattttgacaaaggtcttcaggaaatcaccgacttgatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggaaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccacactaggcccgtatattagtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                                                                                                         >  1:756517‑M2/202‑226 (MQ=255)
              tgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgccctaggcccgaaaattcgtgaaggtaAGTGCAAAAATAACCAATTCTTTttcac                                                                                                                                                                                                      >  2:723822‑M2/202‑227 (MQ=255)
              gtgtatcggaattttgacaaaggacttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcaaaacaggagatagcaatgacgttaccgaggagacacccgaagagcagaatgatcaaaaaatgtacacaactaggccggcaatttcgtgaaagaAATGGACAGATAAAGCGATTCTTTTtca                                                                                                                                                                                                       >  2:387842‑M2/202‑228 (MQ=255)
                      atttttgacaaaggttgttaggaaagccccgtctggatcgaaaaacggttgttttaaaaatttgctaaatttggccaatttggtaaaacaggtgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                                                                                                              <  2:329285‑M2/36‑1 (MQ=255)
                       attttaaaaaagggtgtcaggaaaacaccgacttgatcgaaaaacggttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagaaagaaatgacgttaccgagtggacgcccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                                                                                                              <  2:395760‑M2/36‑1 (MQ=255)
                        ttttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatatcaatgacgttaccgagtggacacccgcagagcagattggtcaaaaaaattaccgcactaggcccgtatattcgggaaggtaAAGGCAAAGATAATCGATTCTTTttcgattggcatggt                                                                                                                                                                                            >  2:219906‑M2/192‑222 (MQ=255)
                        ttttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaagttaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCt                                                                                                                                                                                            >  1:204846‑M2/192‑229 (MQ=255)
                        ttttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaaaagcagattgatcaaaaaatttaccgcacaaggcccgtatattcgggaaggacAGTGCAAAGATAATCGATTCTTTTTcgattgttgggcg                                                                                                                                                                                            >  2:73123‑M2/192‑223 (MQ=255)
                        ttttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcataacaacaggagatagcaatgccgttaccaagtggacacccgaagagcaaattgatacaaaaatttacccacctaggcccggtaattcggcaagtaaAGAGAAAAGATAATCAATACTttttcgatgttttgt                                                                                                                                                                                              >  2:599020‑M2/192‑220 (MQ=255)
                        ttttgacaaagggcttaagaaaatccccgactggatccaaaaacgcttgtgttaaaaaattgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTgg                                                                                                                                                                                              <  1:599021‑M2/36‑1 (MQ=255)
                            gaaaaaggtctgcaggaaaacactgacgtgatcgaaaaacgattctgttaaaaattgtgtaaattttgccaatttggtaaaacaggtgctgaacaacaggagatagcaattacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAt                                                                                                                                                                                        <  1:734889‑M2/42‑1 (MQ=255)
                                aaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgccctaggcccgcatattcgtgaagggaAGTGCAAAGATAATCGATTCTTTTTCGATTTTCTGGCTGAATGGGt                                                                                                                                                                                    >  2:558258‑M2/184‑229 (MQ=255)
                                          ggaaatccccgacttgatcgaaaaacgcgtgggttaaaaatttgctaaattttgccaatttgggaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacaccagaagagcagattgatcaaaaaatttaccgcactaggtccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaaa                                                                                                                                                                          <  2:448530‑M2/56‑1 (MQ=255)
                                              atcaccgactggatcgaaaaacgcttgtgttaaaagtttgctaaattttgccaatttggtaaaacagttgcaacacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaattttccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATGCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                                                                                                                                                        <  1:342054‑M2/58‑1 (MQ=255)
                                              atcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagcttgatcaaaaaatttaccgcactaggcccgtatattcgtgacggtaAGTCCAAAGAAAATCGATTCTTTTTCTATTGTCTGGCTGTATGCGTCAACGTGAAACAgg                                                                                                                                                                      >  1:270038‑M2/170‑229 (MQ=255)
                                                      ctggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCgg                                                                                                                                                                <  1:405911‑M2/66‑1 (MQ=255)
                                                          atcgaaaaacgcttgtgtaaaaaatttgctaaattttgccaatttgggaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGc                                                                                                                                                          <  1:73123‑M2/72‑1 (MQ=255)
                                                            cgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                                                                                                        <  2:204846‑M2/74‑1 (MQ=255)
                                                             gaaaaacgcttgtgttaaaaatttgctaaatttggccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTg                                                                                                                                                       <  1:194973‑M2/75‑1 (MQ=255)
                                                                   cgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggaaacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggcaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGaatttt                                                                                                                                                 >  2:308843‑M2/149‑227 (MQ=255)
                                                                       tttgttaaaaatttgctaaattttgccaatttggtaaaacagttgcataccaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                                                                                                                                             <  2:312985‑M2/85‑1 (MQ=255)
                                                                               aaatttgcaaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgactttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtgga                                                                                                                                     <  1:610890‑M2/93‑1 (MQ=255)
                                                                                aatttgctaaattgtgctaatttggtaaaacagttgcatcacaacaggaaatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaagttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat                                                                                                                                    <  1:306142‑M2/94‑1 (MQ=255)
                                                                                    tgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAg                                                                                                                                >  1:167614‑M2/132‑229 (MQ=255)
                                                                                    tgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGACCCGGAAATGCGGGCATTCTGGGGCTGTGGGATGGAg                                                                                                                                >  1:90923‑M2/132‑229 (MQ=255)
                                                                                     gctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaTGTGCAAAGATAATCGACTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGAACCGGAAGTGCGTGACTTCTGGGGCAGGGGggaggagg                                                                                                                               >  2:240505‑M2/131‑228 (MQ=255)
                                                                                                     ttgggaaaacagtggcatcacaacaggaaaaagcaatgactttaccgagtggacccccaaagagcagattgatcaaaaaagttaccgccctaggcccttatattcgtgaaggtaAGTGCAAAGATAAGCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATc                                                                                                               <  2:270038‑M2/115‑1 (MQ=255)
                                                                                                          aaaacagttgcatcacaacaggagatagcaatggcgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtt                                                                                                          <  1:79739‑M2/120‑1 (MQ=255)
                                                                                                              cagttgcaacacaacaggagattgcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                                                                                                      <  1:617844‑M2/124‑1 (MQ=255)
                                                                                                                 tttcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                                                                                                                                             <  2:79893‑M2/85‑1 (MQ=255)
                                                                                                                 ttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATACTCGATTCTTTTTCGCTTGTCTGGCTGTATTCTTCAACTTGAAACCGGCACCGGAAGTGCGGCAATTCTGGGGGTGGTGGGTGGGGCCGGAAGCCACGGAATCCGGTTTtaccct                                                                                                   >  2:536427‑M2/103‑227 (MQ=255)
                                                                                                                 ttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                                                                                                                                             >  1:79893‑M2/103‑187 (MQ=255)
                                                                                                                     ctcataacaggagatagacatgacgttaccgggtggacacccgaagaggagaatgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGt                                                                                              <  1:387842‑M2/131‑1 (MQ=255)
                                                                                                                       cacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTa                                                                                            <  1:161729‑M2/133‑1 (MQ=255)
                                                                                                                       cacaaaaggggatagcaatggcgttaccgagtgaacccccgaagagcagattgataaaaaaatttaccgcactagggccgtatattcggtaaggtaAGTGCAAAGATAATCGATTATTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTa                                                                                            <  2:368917‑M2/133‑1 (MQ=255)
                                                                                                                           acaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtctattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACTTGAAACCGGCACCGGACGTGCGTGAATTCTGGGGATGGTGAATGGAGCTGGACGCGCAGGAATCCCGTTTTACCTCAAGttcccgg                                                                                        >  1:397595‑M2/93‑227 (MQ=255)
                                                                                                                            caggagaaaagaatgactttaccgtgtggcaacccgaagagcagagtgaacaaaaaatttaccgtactaggcccgtatattcgtgaaggtaAGTGCAAAGCTAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGt                                                                                       <  2:756516‑M2/138‑1 (MQ=255)
                                                                                                                                 gatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGt                                                                                  <  1:723823‑M2/143‑1 (MQ=255)
                                                                                                                                   tagacatgacggtaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGGCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGt                                                                                  <  1:624148‑M2/143‑1 (MQ=255)
                                                                                                                                    agcaatgacgttaccgagtggacccccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTg                                                                               <  1:558259‑M2/146‑1 (MQ=255)
                                                                                                                                    agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGGGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGGCTg                                                                               >  1:569779‑M2/84‑229 (MQ=255)
                                                                                                                                              ttaccgggtggacaaccgaagaaaggattggtcaaaaaatttaccgcaataggcccggatattcgtgaaggtaAGTGCAAAGATAATCGATTCTGTTTCGATTGTCTGGCTGTATGCGACAACGTGAAACCGGCACTGGAAGTGCGTGAATGCTGGGGCTGGTTGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAg                                                                     <  1:422172‑M2/156‑1 (MQ=255)
                                                                                                                                                accgagtggacacccgaagagccgattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTTTGGGGCTGGTGGATGGAGCTGGAAGGGCAGGAATCCCGTTTTACCTACAGTTACCATGTTGGTCTGTTTgataaaaga                                                                   >  2:158591‑M2/72‑226 (MQ=255)
                                                                                                                                                  cgagtggacaccgtaagaggagattgaacaaaaaatttgccgcactaggacctgatattggtgaaggtaAGTGCAAAGATAATCGAGTCTGTGGCGAGTGTCTGGCTGTATGCGTCAGCGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAg                                                                  <  1:717457‑M2/159‑1 (MQ=255)
                                                                                                                                                   aggtggacacccgaagggcaggtttaataaaaaatttcccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGc                                                                <  2:724441‑M2/161‑1 (MQ=255)
                                                                                                                                                     gtggacgcccgaaaagcagattgatcaaaaaatttaccgcactaggcccgtatcttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGCTTGTCTGGCTGTATGGGTCAACGTGAAACCGGAACCGGAAGTGCGTGAAGTCTGGGGCTGGTGGATGGGGCTGGTAGAGCCGGAATCCCGTTTTACCTCCAGTTACAAGTTTGGGGTGTGCGGTAAAGAAGgaga                                                              >  2:145968‑M2/67‑229 (MQ=255)
                                                                                                                                                      tggacaccctaagagcaaattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGAc                                                             <  1:222189‑M2/164‑1 (MQ=255)
                                                                                                                                                      tggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGTTAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGACGCGCAGGAATCCCGTTTTACTTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGAc                                                             >  1:420756‑M2/66‑229 (MQ=255)
                                                                                                                                                      tggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTCCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTTACATTTCCAGTTTGGTCTGTTCGTAAAAACAgacgac                                                             >  2:243471‑M2/66‑229 (MQ=255)
                                                                                                                                                      gggaaccccgaagagcaggttgatcaaaaaatttaccgcacttggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCg                                                               <  2:441482‑M2/162‑1 (MQ=255)
                                                                                                                                                                  gggcaaatttatcaaaaaatttaccgcactaggcccgtatattcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAGGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCGGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAgtgt                                                  <  1:240505‑M2/175‑1 (MQ=255)
                                                                                                                                                                       gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGAGTTcc                                               >  1:187490‑M2/49‑226 (MQ=255)
                                                                                                                                                                          ttatcaaaaaatttaccgcaatgggcccgtgttttgctgaaggtaAGTGAAAAGATAATCGGTTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTaaa                                         <  2:619917‑M2/184‑1 (MQ=255)
                                                                                                                                                                           gatcaaaaaattgaccgtactaggcccgtattagcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGGCGGGCTGTATGCGTCAACGTGAAACCGGAATCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACGTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTaaa                                         <  1:655816‑M2/184‑1 (MQ=255)
                                                                                                                                                                             tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGTTAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAagc                                      >  1:31257‑M2/43‑228 (MQ=255)
                                                                                                                                                                                atgtgtataaggtgcagggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTGCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGaca                                    <  1:251200‑M2/189‑1 (MQ=255)
                                                                                                                                                                                  aaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGAGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCCGTTTGGTCTGTTCGATAAAGCAGGCGACTGGCAGAGTGTTCCGGGAAAAGACCCTg                                 >  1:431104‑M2/38‑229 (MQ=255)
                                                                                                                                                                                       taccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGTATACCGTTTTACCTACAGTTACCAGGTTGGTCTGTTCGATAAAGCAGGCGACTCGAAGAGTGTTCCGGTAAAAGACACTGCAGTg                            >  1:333341‑M2/33‑229 (MQ=255)
                                                                                                                                                                                       taccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGGTAAAGCAGCCGCCTGAGAGAGGGTACCGGTAAAAGACACTGAAGTg                            >  1:333346‑M2/33‑229 (MQ=255)
                                                                                                                                                                                             actaggcccgtttattcgttgggggaAGTGCAAAGATTATCGAGTCTTTTGCGAGTGTCTGGCTGGAGGCGTCAGCGTGAAGCCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCGGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGaa                      <  2:488528‑M2/203‑1 (MQ=255)
                                                                                                                                                                                                tgccccgttttttggggAAGTTAAGTTCAAAGATTATCGGTTCTTTTTGGATTGTCTGGCTGGATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGa                   <  2:569778/213‑1 (MQ=255)
                                                                                                                                                                                                aggcccgtatattcgtgaaggtaAGGGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGa                   <  1:536427‑M2/206‑1 (MQ=255)
                                                                                                                                                                                                  gcccgttttttcgggaaggtaAGGTCAAAGATAACCGGTTCTTTTTCGATTGGCTGGCTGTATGCGTGAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACt                 <  2:187490‑M2/208‑1 (MQ=255)
                                                                                                                                                                                                  gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACt                 <  1:501171‑M2/208‑1 (MQ=255)
                                                                                                                                                                                                       tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTTAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAACCGGCGACGGGAAGAGTGTTCCGGTAAAATACACTGAAGTGGTTGAACGACTGGaga            >  2:687330‑M2/17‑228 (MQ=255)
                                                                                                                                                                                                       tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCGGTTTGGTCTGTTCGATAAAGAAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTTAAGGGGTTGAACAACCGGGGc            >  1:631356‑M2/17‑229 (MQ=255)
                                                                                                                                                                                                       tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAATAGTGTTCCGGTAAAAGCCACTGAAGTGGTTGAACGACTGGAGc            >  1:616731‑M2/17‑229 (MQ=255)
                                                                                                                                                                                                       tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACCACGTGAGc            >  1:69955‑M2/17‑229 (MQ=255)
                                                                                                                                                                                                        attttgggtaaggtaAGTGAAAAGATAATCGATTCTTGTGCGATTGGCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGca           <  2:74489‑M2/214‑1 (MQ=255)
                                                                                                                                                                                                        atattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGca           <  1:414051‑M2/214‑1 (MQ=255)
                                                                                                                                                                                                           ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGATTCCCGTTTTACCTACAGTTACCAGTTTGGTCTTTTCGATa                                                                        >  2:341066‑M2/13‑165 (MQ=255)
                                                                                                                                                                                                           ttcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATa                                                                        <  1:341066‑M2/153‑1 (MQ=255)
                                                                                                                                                                                                            tcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGGTGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACCGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCCACTGGAAGAGTGTTCCGGgaaaagaaactagagaggttcggtctcttaaaaaaatc       >  2:251200‑M2/12‑211 (MQ=255)
                                                                                                                                                                                                                 aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGCTGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGGGGTTGCACGACGGTAGCCAACCCTACg  >  1:354622‑M2/7‑229 (MQ=255)
                                                                                                                                                                                                                 aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAACGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACTTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGGTTTCCGGTAAAAGCAACCGACGAGGTTGAACGACTGTAGaaaacccgtcg  >  2:524121‑M2/7‑225 (MQ=255)
                                                                                                                                                                                                                       |                                                                                                                                                                                                                                
CCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCG  >  NC_000913/258469‑258906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: