Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 12,484 | C→A | 19.6% | Q108K (CAG→AAG) | dnaK → | chaperone Hsp70, with co‑chaperone DnaJ |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 12,484 | 0 | C | A | 19.6% | 112.7 / 4.2 | 63 | Q108K (CAG→AAG) | dnaK | chaperone Hsp70, with co‑chaperone DnaJ |
Reads supporting (aligned to +/- strand): ref base C (46/4); new base A (2/11); total (48/15) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.49e-07 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAG > NC_000913/12258‑12712 | cGATCGCACCACGCCTTCTATCATTGCCTATGCCCAGGATGGTAAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCa < 1:688979/228‑1 (MQ=255) cGCACGACGTCTTCTATCATTGCCTATATCCAGGATCGGGAAACTCTAGTTGGTCAGCCGGCTAAACGTCTGGCAGTGACGAACCACCAAAACACTCTGGTTGCGATTAAACGCCTGATTGGGCGCCGCGTCCAGGACCCAGAAGGACAGACGGAAGTTTCCATACTGCCGCTCAAAATTATTGCGTCGTAT‑AA‑CGGCGCCACAAGGG‑TCGAAGTTAAAAGCCACaaa > 2:498516/1‑228 (MQ=255) cacGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAAAG‑GCa > 2:517683/1‑229 (MQ=255) tGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAGACAATCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGGTTCCATCATGCCGTTCCAAATTATTGCTGCTGAT‑AA‑CGGCGACCCCTGGG‑TCGAAGTTAAAGTCCAGAAAATG‑GGA‑CCGCCGGCAATTTct > 2:331740/1‑229 (MQ=255) tGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGAttt > 1:258592/1‑227 (MQ=255) ccTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGAGGCATGGG‑TCGAGGTTAAAGGCCGAAAAATG‑GCA‑CCGCGCCAAATTTcggc > 2:86078/1‑229 (MQ=255) tataCCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTCCAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCCTGGG‑CCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCACCCCGGATTTctgcgg > 2:189853/1‑227 (MQ=255) tataCCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCCGATTTcttctg > 1:392156/1‑229 (MQ=255) taCCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGTTGAT‑AA‑CGGCGACGAATGTG‑TCGACGTCAAAGGACAGAAAATG‑GAA‑ACCCCGCAGATATATGCTGaa > 2:312712/1‑229 (MQ=255) cAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGT‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGCAGTGc > 1:186169/1‑229 (MQ=255) ggATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTACACGCATGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TAGAGGTTAAAGGCCAGACAATT‑GAA‑CCGCCGCAGAtctccgtggaagggttg > 2:668389/1‑216 (MQ=255) ggATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGCT‑AA‑CGGCAACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGAGGATTTCTGCTGAAGTGCTg > 1:63047/1‑229 (MQ=255) gATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TGGAGGTTAAAGGCCAGAAAATG‑GAA‑CCGCCGCAGATTTCTGCTGAAGTGCTGa > 1:679708/1‑229 (MQ=255) ggTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCACCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCCTGCCGTTCCAAATTATTGCTGCTGAT‑AA‑CGGCGACGCCTGGG‑TCGAAGTTAAAAGCCAGAAAAGG‑GCA‑CCTCCCGAGATTTTTGCTGaaaagggtaaac > 2:75483/1‑219 (MQ=255) tGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGaaaaa > 1:80683/1‑228 (MQ=255) tAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCCGATTTTTGCTGAAGTGCTGAAAAAAAAaaagaa > 2:606135/1‑229 (MQ=255) aGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGCCGCATGGC‑TCGAAGTTAAAGGCAAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAAAGaaaaaa > 2:723555/1‑229 (MQ=255) aGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGACAATG‑GCA‑CCGCCGCAGATTTCGGCTGAAGTGCTGAAAAAAA‑AAAGaaaa > 2:597945/1‑229 (MQ=255) caccgcGGCAAAACGCCAGGCAGTGCCGACCCCGCAAAACATTTGGTTGAGGATAAAACGCTTGGTTGGCCGCACGTTCCGGGACAAAAAAGTACAGGGTGAGTTTTCCAGCAGCCCGTAC‑AAATTAATGGCGCTGAA‑AACCGGCGACGCTGGGG‑CAGAAGTAAAAGACAAAAAAAGG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCt < 2:645265/224‑1 (MQ=255) cAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCTTCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCAAGAAAATG‑GCA‑CCGCCGCAGATTTATGCGGAATTGCTTAAAAAAATGAAGAAAACCg > 1:143244/1‑226 (MQ=255) cGGCAAAACGTAAGGCAGTGACCACCCCGAAAACCACTCTGTTTGCGATTAACCGCCTGATTGGTCGCCGCTTCCAGGACGAAAAAGTACAGCGTGATGTTTCCATCATGCCGTTAAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCAAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGa < 1:597946/229‑1 (MQ=255) aGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAAGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTCTTGCTGCTGAT‑CA‑CGGCGACGCCTGGG‑TCCAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGGGCTGCAAAAAATTAAGAAAACCGCTGAAGCTAACCTGGGTGa > 1:189713/1‑229 (MQ=255) tGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCC‑CCGCCGCAGATTTCTGCTGAGGGGCTGGAAAAAATGAAGAAAACCGCTGACGATTACCTGGGTGAACCggg > 1:346458/1‑228 (MQ=255) aCCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CACCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCGGCAGATTTCTCCTGAAGGGCTGAAAAAAATGAAGAAAACAGCTGACAGTTACCTGGTGGAACCGGTAACTg > 1:72725/1‑228 (MQ=255) gCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGACGATTTCTGCTGAAGTGCTGGAAACACTTAAGAAAACCGCGGAAGATTAACTGGGTGAACCGGTAACTGaaac > 1:331625/1‑226 (MQ=255) gCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑ACGCCGCCGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAAGTTACCCGGGTGAACCCGGCACTTAAGca > 1:347086/1‑228 (MQ=255) ctctGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATAAAGAAAACCGCTGAAGATTACCTGGGGGAACCGGAAACTAAAGCGGTTaaaac > 2:119008/1‑225 (MQ=255) tctGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGGGACCCGGTAACTGAAGCTGTTAtaaac > 1:510022/1‑225 (MQ=255) tttGCGACTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGAGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAA‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGAACAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTACTGCAAAAACTGAAGAAAACCGCTGAAAATTACCTGGGGGAACCGGAAACAGaaccgataagaacagcaa > 2:493921/1‑213 (MQ=255) ttGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAACATTATTGCTGCTGAT‑AA‑CGGCGATGCATGGG‑CCGAATTTAAAGGCCAGAAAATG‑GCA‑TCGCCGCAATTTTCTGCTGAAGTGCTGCAACGAATGAAGAAAACCGCTGCAAATTACCTGGGTGAACCGGTTACTGAATCtttaataccagacca > 2:360736/1‑215 (MQ=255) gCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCg > 1:623303/1‑228 (MQ=255) gCCTGATGGGCCCCCGTTTCAGGGACGAAGAAGTAGAGGGGGATTTTTCCATCATGCCGTTAAAAATTATTGCTGCTGAT‑AA‑CGGCGACGTAGGGG‑TAGAAGTAAAGGGCAAGAAAAGG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTaa < 1:119008/229‑1 (MQ=255) cTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAACAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAACCTGTTATCACCGTACCAGCAAACTTTaaa > 2:259967/1‑227 (MQ=255) tggcccccgtTTCAAGGAGCAAAAAATACAGGGTGATTTTTCCATCATGCCTTACAAATTTATTGCTGTTGAA‑AA‑GGGCGCGGAAGGGG‑CCAAAGTAAAAGGCAAAAAAAGG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCt < 1:150810/223‑1 (MQ=255) gggTCGCCGTTTCCAGGAAGAAAGAATACAGGGTGATGTTTCAAATGTGCCTTTCAAAATTATTGAGGAGGAT‑AC‑GGGCGACGCGTGGG‑TCAAAGTTAAAGGCAAAAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCt < 1:501073/228‑1 (MQ=255) gccgcTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCCGATTTCTGCTGAAGGGCTGAAAAAAATGAAGAAAACCGCTGAAGCTTACCTGGGTGAAACGGTAACTGAAGCGGTTAAAACCGAACCGGCATCCTTTAACGAttttcaagc > 2:407237/1‑221 (MQ=255) ttCAAGGAGAAAGAAGCAGAGGGGTATGTTTCCATCGTGCCTTTAAAAATTATTGCCGGTGAA‑GA‑CGGGGAGGGAGGGG‑CAGATG‑TAAAGGCCAAAAAAAG‑GGACCCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAgg < 2:49290/229‑1 (MQ=255) gtcgaGGACGAAAAAGGACAGCGTTATGTTCCGTACAGGCCGGAAAAATTTATGGC‑GGTGATAAA‑CGGCGACGAAGGGT‑GCGAAGTAAAAGGCAAGAAAAGG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAgg < 1:440333/225‑1 (MQ=255) aCGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGGGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCa > 1:57455/1‑197 (MQ=255) tACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATGTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTTGGTGAACCGGTAACGGAAGCTGTTATCACCGTACCGGCATACTTTAACGATTCTCAGCGTCAGGAAACCAAAGACACAgg > 2:743912/1‑229 (MQ=255) aCAGCGTGATGTTTCCATCATGCCGTAAAAAATTATTGCTGCTGAA‑AA‑CGGCGCCGAATGGG‑TAAAATTAAAAGCCAAAAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTGCCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCa < 1:86078/226‑1 (MQ=255) cAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGCAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGACAATTACCTGGGTGAACCGGTACATGAAGCTGTAATCACCGTAACGGCATACTTTAACGCATCTCAACGCATGGCAACAAAAAACGCAGGc > 2:74146/1‑228 (MQ=255) gCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATAACCGTACCGGCATACTTTAACGATGCCCAGCGTCAGTCAACCAAAGACGCAGGcc > 1:420252/1‑227 (MQ=255) cGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGCAAAACATGAAGAAAACCGCTGAAGATTACCTCGGTGAACCGGGAACTGAAGCCGTTACCAACGTACCGGCATACTTTAACCATGCTCAGCGTCACGCACCCAAAACCGCAGccc > 2:299299/1‑226 (MQ=255) cGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCt > 1:748810/1‑132 (MQ=255) cGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGcc > 1:387407/1‑226 (MQ=255) cGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACATGGTTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCAGACTTTAACGAGGCTCAGCGTCCGGCAACCCAAGACTCAGGccatc > 1:611579/1‑226 (MQ=255) tGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCa > 1:513023/1‑181 (MQ=255) tCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGAGGCTCAGAGTCAGGCAACAAAAAACACAGGCCGTAACCCTggac > 2:387884/1‑227 (MQ=255) cccATCATGCCTTACAAAATTATGGCTGGTGAT‑AA‑CGGC‑TGGGATGGGATGGAATTTAAAGGGAAGAAATTG‑GTA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTc < 1:712406/228‑1 (MQ=255) tcatGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGa > 1:616846/1‑229 (MQ=255) gCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGGAACTGAAGCTGTTCTAACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGGTTGGAAGTa > 1:739961/1‑229 (MQ=255) ttCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGGGAACCGGTAACTGAAGCTGTTATCACCGAACCGCCATCATTTAACGATCCTAAGCGTCAGGCAACCAAAGACGCAGGCCGCATGGCTGGTCTGGAAGTaaaa > 1:108594/1‑228 (MQ=255) cAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGt > 1:370212/1‑229 (MQ=255) aatttgtggcggatgaaaccGGGGAGGCAGGGG‑TGAAAGTAAAAGACAAAAAAATG‑GAC‑CCGACGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTat < 1:2271/210‑1 (MQ=255) tgAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTCCCGGCATACTTTAACGATGCTCAGCGCCAGGCAACCAAAGACGCAGGCCGGATCCCTGGTCTGGAAGAaaaaacaaacaacaacaaacca > 2:372609/1‑211 (MQ=255) gAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGCCCAGAAAATG‑GCA‑CCGCCGCACATTTCTGCTGAAGAGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGAACATGAAGATGTTATCACCGGACCGGCATACTTTAACACTGCTCGGCGTCAGGCAACCAATGACACAGGCCGTATCTCTGGTCTGGAGGTCAAACgaacacgcaaacaaccga > 2:91877/1‑212 (MQ=255) cGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTggcc > 1:384470/1‑186 (MQ=255) cctggtggtgagaGTTAAAGAACCAAAAATG‑GCC‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAAGGAAGAAAACCGGTGAAGATTACGTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTgcg < 2:679707/217‑1 (MQ=255) ggttcagagtAAAGGCCAAAAAACGCCCC‑CCGCCGCAGATTTCAGCTGAAGTGCGTAAAAAAATGAAGAAAACCTCTGAAGATTACCGGGTGGAACCGGTAACGGAAGCCGTTATCCCCGTACCGGAATACTTTAACTATGCTCAGCCTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCt < 2:580881/217‑1 (MQ=255) ccaaaGTAAAAGGCAAAAAAATG‑CCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCtt < 1:570656/225‑1 (MQ=255) caaatttAAAGGCAAAAAAGTG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTa < 1:517683/223‑1 (MQ=255) aaGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGGATCATCAACGAACCAACCGCAACTGCGCTGGCTTAAGGACTAGa > 2:156873/1‑229 (MQ=255) cAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGCACGCAGCTGCGCTGGCTTACGGACGGGAAAAAg > 2:737516/1‑229 (MQ=255) | CGATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAT‑AA‑CGGCGACGCATGGG‑TCGAAGTTAAAGGCCAGAAAATG‑GCA‑CCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAG > NC_000913/12258‑12712 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |