| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 715,338 | T→A | 14.2% | intergenic (+140/‑74) | pgm → / → ybfP | phosphoglucomutase/lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 715,338 | 0 | T | A | 14.2% | 164.3 / 3.6 | 68 | intergenic (+140/‑74) | pgm/ybfP | phosphoglucomutase/lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (42/15); new base A (2/9); total (44/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.65e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTG > NC_000913/715128‑715562 | gttaagaaCATCGGAAGCAGATTGAAAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACCCA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCAt < 1:96546/226‑1 (MQ=255) tGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAAGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGACTGATGCGTTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGGATTTAAGCGATTTTGTAAGC‑CGGATAAGGC‑GTTAACGCCGCATc > 1:448155/1‑229 (MQ=255) aaCATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAACGATTTTGTAGCC‑CGGATAAGGC‑GTCCACGCAGCATCCGGcc > 1:432867/1‑228 (MQ=255) aTCGCAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTGAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCaaaa < 1:571026/229‑1 (MQ=255) cGCAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAaac > 1:651338/1‑229 (MQ=255) cAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGCGGATGGTGCAATATATTGACTTTAAGCGTTTTTGTAGGC‑CGGATAAGGC‑GTTCACGCGCCATCCGGCCAAaacaa > 1:473433/1‑229 (MQ=255) aTTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGGTAAGGC‑GTTCACGCCGAATCCGGCAAAAAAAACGAaaac > 2:64285/1‑229 (MQ=255) aagCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGCTTTTGTAGGC‑CGGTAAAGGC‑GTTCAGGCCGCATCCGGCAAAAAAAACGAAAAATTTTAcaac > 2:286820/1‑228 (MQ=38) agCTGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑ATTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑AGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTcaacaa < 1:375314/229‑1 (MQ=255) agggTTTAGATTTGTTAGCGAAGTTTGTAAAACCGGGTAAACTCA‑TTTAAAAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGAAAAACACAGAATTGCCTGATGGGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGGTAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTcaacaa < 2:52135/227‑1 (MQ=38) gaga‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGAAAAGGC‑GTTCACGCCGCATCCGGCAAAAGCAACGAACACTTTGTCAACAAAccaaga > 2:445466/1‑224 (MQ=255) ttAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAACTCaa > 1:256011/1‑229 (MQ=255) ccGAATTTCTCAAACACGCTTAAA‑ACATTTTAAAAAAAAAAGGGCCGGCGTAAGATCCACATTTTTTACTTAGGACAAACACAGAAATGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGTAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGa < 2:568844/228‑1 (MQ=21) tCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCCGGCCTACGAGGATGGTGCAATATATTGAATTTAACCGATTTTGTAGTC‑CGGCTAAGGC‑GTTCACGCCCCATCCTGCAAAAACAACAAACACTTTGTCACCAACCTGAgaggccacgggaaatccaa > 2:611225/1‑211 (MQ=255) gAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACTCTTAGCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATg < 1:624072/229‑1 (MQ=255) caaggaggtgtttaagagaacaaaaaaaGGGGGGGCGTAAGATCGCCCTATAGTACGTAGGACAAATGTAGAATTGCCTGATGCGCTACGCTTAGCAGGCCGACGAGGATGGTGCAATATATACAATTTAAGCGATTATGTAGGC‑CGGATAAGGC‑GTTCAAGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAAGCCCAATGAAg < 2:8900/208‑1 (MQ=255) cgcgTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCTGCAAAAACACCGAACACTTTGTACAAAAACTGAGTAGATCAACGAAAACCAAATaaaaaac > 2:214086/1‑225 (MQ=37) cgcgTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGAAGCTCAAGGAAATCCCAATGAAGa > 1:346693/1‑227 (MQ=255) cgcgTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAATAGCCCAAGGAAAACCCAATGAAAAca > 2:280876/1‑228 (MQ=255) cgcgTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTAAGTAGCCCAAGGAAATCCCAAAGAAAAcc > 2:422247/1‑229 (MQ=255) cgTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATAAAGACCaa > 1:499886/1‑229 (MQ=255) ttaaCACA‑TTGAAAAAAAAAAGGGCTGTTCCAAGATCGCTCTTTTTTCCGGAGTCCAAACATATAATTGCTTGATTGGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGTC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACa < 1:744314/227‑1 (MQ=37) ttAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTCCGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGCTAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCACAAAACTGCGGAGCTCAAGGAAAACCAAAGAAAGACCAACAagagaagtg > 2:756026/1‑224 (MQ=37) aaaaaaGGGCGGTCGCAAGGTCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCa < 1:411911/229‑1 (MQ=255) aaaaGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGAAAAAACAACGAACACTTTGTCACCAAACTGAGTAGCTCAAGGAAATCCCAATGAAGCCCAACAGGAGACTGGCCGTCCTa > 1:334582/1‑229 (MQ=255) gCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGt > 1:625598/1‑211 (MQ=255) gCGGTCGCAAGATCGCCCTTTTTTACGGATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGt < 2:625597/211‑1 (MQ=255) cgttCGCAAGAGCGCCTTTTTTTACGTATGACAAACACAGATTTGGCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGAAAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCAc < 2:334582/226‑1 (MQ=255) ggTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGCCAAAAGGTCACt > 2:340060/1‑229 (MQ=255) gTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAg > 1:8900/1‑196 (MQ=255) tCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATAAAGACCAACAGGAGTCTGGGCGTCATAGTGTCACTaa > 1:311813/1‑229 (MQ=255) tCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCAATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCACCAAACTGAGAAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATATGGTCACTac > 1:338225/1‑228 (MQ=255) tccgctttttttACATTTGACAAACAAAGAATTGCTGGATGCGCTGCGCTTATGAGGCATACGAGGAGGGGGAAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGAAAAAGCAACGAACACTTTGCCAACAAACTGAGTAGCCCAAGGAAATCCCAATGAAGCCCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGc < 1:445466/222‑1 (MQ=37) ccTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTTGAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACCGAGTAGCCCAAGGAAATCCCAATGAAGACCAACAGGAGCCTGGTCGGCATAgtggccccaataaaagccaaca > 2:563831/1‑210 (MQ=25) ttttACGTATGACAAACACAGAATTGCATGATGCGCTACGCTTATCAGGCCTACGAGGTTGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGCTAAGGC‑GTTCAGGCCGCATCCGGCAAAAACAACGAACACTTTGTCACAAAACTGAGTCGCTCAAGGAAATCCCAATGACAACCACCAGGAGTATGGTCGTCATAGTGCAAGTACTTACCGcaccatgat > 1:315891/1‑220 (MQ=25) gACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCACCAGGAGTCTGGTCGTCATAGTGTAACAAATTACCGCCACATTACTGCTGACttc > 1:205877/1‑226 (MQ=255) cTGATGGGCTACGTTTACCGGGCCTAAGGGGATGGTGCAATTAATGGAATTAAAGCTATTTTGAAGGC‑CGGAAAAGGC‑GTTCACGCCGCATCCGGAAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGCCCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACa < 1:64285/229‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGACGTCATAGTGTCACTAATTACCGCAACATTACTGCTGACTGCATGCGCCCAACCTGAACa > 1:543375/1‑226 (MQ=255) tGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGGCTGGTCGTCATACTGTCACTAATTACCGCCCCATTACTGCTGACTGCATGCGCCCAACCTGAACAatc > 1:116748/1‑228 (MQ=255) ccTACGTTTAACAGGCCTACGGGGATGGTGCAATATATGAAATTTAAGCGATTTTGTAGGC‑CGTAAAAGGC‑TTTCACGCCGCATCCGGAAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAAtcatca < 1:422247/228‑1 (MQ=255) attacctgacgaggcactcGAGGGTGGTGCAATATAGTAAATTTAAGCAATTTTTGAGGC‑CGAATAAGGC‑GTTCCAGCCGGATCCGGCAAAAACAACGAACACTTTGCCAACAAACTGAGTAGCTAAATGAAATCCCACTGAAGCCCAACAGGATGCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATcatcat < 1:286820/211‑1 (MQ=255) aTCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGCCTGCATGCGCCCAACCTGAAAAATCACCATTAGCTg > 2:481984/1‑227 (MQ=255) ggCCTACGGGGGGGGCGAAATATATTAATTTAAAGCGATTTGGTAGCC‑CGGAAAAGGT‑GTACACGCCGCATCCGGAAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGCCCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACt < 1:340060/229‑1 (MQ=255) gCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACt > 1:133682/1‑228 (MQ=255) gCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGACTGGTCGTCATAGTGTCACTAATTACCGCCACATAACTTCTGACTGCATCCGCCCAACCTGAACAATCATCATAAACTGGCGAcg > 2:655613/1‑227 (MQ=255) tACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGtt > 1:594494/1‑229 (MQ=255) cGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTAc > 1:459613/1‑229 (MQ=255) cGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTACACAACCATCATTAGCTGGCGACTGGTTAc > 1:407763/1‑229 (MQ=255) ggagagggtaaaaTATATTAAATTAAATAAATTTTGTAGGC‑CGAAAAGGGC‑CTCCACCACGCATCGGGAAAAAAAAACGAACACTTGAGCAACAAACTGAGTAGCTCAAGGAAACCCCAAGGAACACCAACAGGAGTCTGGTCGTCATAGTGTATCTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACt < 2:499886/218‑1 (MQ=255) gTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GGTCACGCCGCATCCGACAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATACCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCAACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTACCTGGCGACTGGTTACTGAcccc > 1:629757/1‑228 (MQ=255) tacAAGATATTGAATTTAAGC‑AATTTGGAGGCGCGAAAAAGGT‑TTGCACGCAGCAGCGGGAAAAAACAACAAACCTTTTGCAAACAAACGGAGGAGCTAAAGGAAAGCCCAATGAAACCCAACGGGAGTCGGGTTGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTa < 1:214086/227‑1 (MQ=255) gaaaataatTGAATTTAGGCGATTGTAAAGCC‑CGAAAAAGGC‑GTTCCCGCCGCATCCGGCAAAAACAACGACCACTTTGTAAACAAGCTGAGTAGCTCAAGGAAATCCCAATGAAGCCCACCAGGGGTCTGGTCGTCATGTTTTCCCTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTa < 1:535208/221‑1 (MQ=255) atatatTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAgc > 1:371009/1‑228 (MQ=255) atatTTAATTTAAGCGATTTTGTAGGC‑CGAATAAGGC‑GTTAACTCCGCAACCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGAAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTGGGCATCGTGTCACTAATTACCGCCACATTCCAGCTGGCTCCAGGCGCCCAACCTGAACTAGCATCAGTAGCTAGCGCCCGGTAACTGACCCCAAAAGaaa > 2:690943/1‑227 (MQ=255) atataaatataacggttttGTAGGC‑GCGATAAGGCATTTCCCCCCACCACCAGAAAAAACAACGAGCCTTTTGACAACAGCCTGAGTATGTCAAGGAAATCCAAATGAAGACAAGCAGGGGTCTGGCCGTCATAGCTCCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATaa < 2:40657/214‑1 (MQ=255) tGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCACAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGCCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTCCTGACTTAATGCGCCCAACCTCAACAATCAGCATTAGCTGGCTACTGGTTAATGACCCCTAAAGTTAAAAc > 1:515147/1‑229 (MQ=39) tGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAg < 1:8448/83‑1 (MQ=255) tGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAg > 2:8448/1‑83 (MQ=255) tGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCTACTGTTTACTAACCCCTAATGATAAAAc > 1:672384/1‑229 (MQ=255) ttAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAACCCagag > 1:482828/1‑229 (MQ=255) aaGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAGCa > 1:430393/1‑177 (MQ=255) aaGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGAGTCACTAATTACAGCCACATTACTGCTGACTGCATGCGCCCAACCTGACCAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAACCCACAGGa > 1:1628/1‑229 (MQ=255) aaGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTATGGTCGTCATAGTGTCACTAATTACCGCCACATTACTCCTGACTGCATGCGCCCAACCTGAACAATCCTCATTAGCTGGGGACTGGTTATTGACCCCTAAAGATaaaaacctgtc > 2:535208/1‑221 (MQ=255) gtgttttGGGGGC‑CGGAAAGGGC‑GTCCCGCCCGCAGCCGGCAAAAACAACAAACCGTTGTTCAACAAACTGTGTAGCCCAAGAAACCCCCAAGGAAGACCAGCGGGTGGCTGGTCGTGATAGTGTCACTAATTTCCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTa < 2:515147/226‑1 (MQ=255) ctaTTTTATAGGC‑GGAATAAGGG‑TTTCCGCCCCCATCGGGAAAAAAAAACGAACCCTTTGCCAAAAAACTGGTTAGCCAAAGAAACCCCCAATGAAGCCCAACAGGAGTCTGGTCGTCATAGTTTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTa < 2:594493/227‑1 (MQ=255) c‑cGGAAAAGGG‑TTTCCCGCCGCATCCGGAAAAAACAACGAACACTTTGTCAACAAACTGATTAGCTCAAGGAAATCCCAATGAAGCCCAACAGGAGTCTGTTCGTCATAGTGTCATTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCAtt < 1:280876/229‑1 (MQ=255) cGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCag > 1:551446/1‑209 (MQ=255) cGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATACCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTCCTGACTGCCTGCGCCCAACCTGAACAATCATCATTAGCTGGCGCCCGGTTCCTGACCCCTAAACATAAAACAAGAGGATTAAAgggaaaagttg > 2:648586/1‑221 (MQ=255) | GTGAAGAACATCGCAAGCAGATTGAGAAAGAAGCGGTTGAGA‑TTGTTAGCGAAGTTCTGAAAAACGCGTAAACACA‑TTTAATAAAAAAAGGGCGGTCGCAAGATCGCCCTTTTTTACGTATGACAAACACAGAATTGCCTGATGCGCTACGCTTATCAGGCCTACGAGGATGGTGCAATATATTGAATTTAAGCGATTTTGTAGGC‑CGGATAAGGC‑GTTCACGCCGCATCCGGCAAAAACAACGAACACTTTGTCAACAAACTGAGTAGCTCAAGGAAATCCCAATGAAGACCAACAGGAGTCTGGTCGTCATAGTGTCACTAATTACCGCCACATTACTGCTGACTGCATGCGCCCAACCTGAACAATCATCATTAGCTGGCGACTGGTTACTGACCCCTAAAGATAAAACCAGAGGATTAACGGGTAGCATTG > NC_000913/715128‑715562 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |