Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,388,666 | A→C | 16.9% | V49G (GTG→GGG) | tpx ← | lipid hydroperoxide peroxidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,388,666 | 0 | A | C | 16.9% | 143.5 / 2.3 | 68 | V49G (GTG→GGG) | tpx | lipid hydroperoxide peroxidase |
Reads supporting (aligned to +/- strand): ref base A (31/22); new base C (2/11); total (33/35) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.12e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGC > NC_000913/1388448‑1388884 | ttGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGtt < 1:590809/228‑1 (MQ=255) gtGCAACACCTGAAGCTTGCAGAAATTCGGCGGTACGGAAAGGGGAGAGGGTGATAACGTTGTTCAGACCGTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGtt < 1:666683/227‑1 (MQ=255) cGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCATCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCaaa < 1:623686/229‑1 (MQ=255) cGTAAGAGTGCAGAAAATCAGCGTGACGGAATGTGGAGAGGGTGATAACTGTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGGGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCaaa < 1:345926/229‑1 (MQ=255) tGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAACAACCGGTATCAATACTCGGGAAAATGTTCAGCCCTTTTCGTTTACCCGCAAAcgtcccgc > 1:549183/1‑222 (MQ=255) tGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCACACCTTTGAGTTTACCCCCAAACTGACCGa > 2:713945/1‑229 (MQ=255) tGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGAGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACGGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATTGTCATAACTTTGCGTTTACCCCAAAACTGAccag > 2:586160/1‑227 (MQ=255) cAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCGCCAAACTGACCGAgg > 1:88874/1‑229 (MQ=255) agagGGTGATAACGTTTTTCAGACCTTCCGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGGGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCAc < 1:77815/229‑1 (MQ=255) agacgtttaTAACCGTGTCCAGGCATTCTGCGACGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGTACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTGCTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCCATTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCAc < 1:327284/221‑1 (MQ=255) gggTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACga < 1:130875/228‑1 (MQ=255) cttcttCAGCCCTTATCCGCCCGAGAAACGGGGCTGGGGGAACGGCAGATCGGCAGAGATACACAGCCCAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGt < 2:702008/224‑1 (MQ=255) tgttCAGACCTTCTGCGCCCCAGAAACGAGTCGGGGCGAACGGCAGATCGGCAAAGTTACACAGCACAACGGTGTTGGCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGGTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCt < 2:745061/228‑1 (MQ=255) gttCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTCCGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGAAAGATCTTTTGCCACGAGAGAAAAAATCTg > 2:607877/1‑228 (MQ=255) gttCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTg < 1:607878/228‑1 (MQ=255) gttCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTAAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGGGACGTCAGACAGATCTTTTGCCACGAGAGTCAAAGTCTg > 1:254270/1‑228 (MQ=255) aCCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAACCTGACCAGGGGTGAGTTCAGACAGATCTTTTGCAACGAGAGTAAAAGAATGCGCttgg > 2:630208/1‑227 (MQ=255) aCCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGAAAGATCTTTTGCCACGAGAGTAAAAATCTGCGCt > 1:510315/1‑226 (MQ=255) cTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCCGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTAcc > 1:29115/1‑229 (MQ=255) cTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGAACTTTTGCCACGAGAGTAAAAGCCTCGGCTTTGCTAcc > 2:376053/1‑229 (MQ=255) cTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGGATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAggg > 2:287284/1‑177 (MQ=255) cTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGAGTTGTAGATCTCAGATGCCCGTTGGG‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTTCGTTTAACCGCAACATGACCAAGGCAGACGTCAGACAAAGCTTTTGCCACTGCACTAAAAGACTTGACTTTGCTAcc > 1:351499/1‑229 (MQ=255) gcAGAAACGACGCTGGGCGAACGGCAGATCGGCAGGGATACACAGCAAAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGGGGCCCAAACCCCGGTATCAATCCTGGGGAAAATGTTCAGCCTTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCAAGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTg < 2:254270/228‑1 (MQ=255) cAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGGGACGTCAGCCACATCTTTTGCCACGAGAGTAAAAGCCTGGGCTTTGCAACCCACCTggg > 2:282712/1‑227 (MQ=255) aaccggaaccgggCGAACGGCAGTTCGGCAGAGATACACAGCACAACGGTTTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACGTATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATTTTCAGCCCTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGa < 1:178664/219‑1 (MQ=255) gggCGAACGGCAGATCGGCAGAGATACCCAGACCAACGGTTTTGCCGATCTCAGTTGCCAGTTGGT‑TAACCTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAACCTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTCCCCGCCTGCGGGATGGAATTGGc < 1:586161/229‑1 (MQ=255) gggCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGGAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGc > 1:683239/1‑229 (MQ=255) gCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTTCCACGAGAGTAAAAACATGCGCTTTGCTACCCGCCCGCGGGATGGAAATGGCGa > 1:751653/1‑229 (MQ=255) agtgttaccaacgcacaCGGTTGTGTCCACCTCGTTTGCCAGTTGGT‑TAAATTTCCGTACTGATGCGGCGCAAACCCCGGTATCAAGACCCGGGAAAATGTTCAGCCTTTTGCGTTTACCCGCAAACGGACCGAGGGTGACGTCAGACAGATCTTTTGCCAGGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGccc < 2:751652/213‑1 (MQ=255) aattaccaaGCAAAACGGTGGTGTCAGTCTCAGTTGTCATTTGGT‑TAAACTTACGGACTGATGCGGCGCAAACACCGGTACAAATACTCGGAAAATTGTTCAGCACTTTGCGTTTCCCCGCAAACTGACCGGGGGTGCCGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTg < 2:522146/221‑1 (MQ=255) gtccaactcccaccGGTGTTGTCGATCCAAGTTTCAAGTTGGT‑TAAACTTACGTCCGGATGCGGCGCACACCCCGGTATCAATACGCGGGAAAATGTTCAGCACTTTGCGTTTCCCCGCAAATTGACCGAGGGTAACGTCAGACAGATTTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGa < 2:618806/216‑1 (MQ=255) caGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACCGATCTTTTGCCACGAGAGTAAAAGTCGGCGCTTTGCTACCCGCCTGCGGGTTGGAATTGGCGACTGTAACCGGGTTGCCCGGGAAAAGa > 2:370693/1‑229 (MQ=255) ggagacgggtggtGGCGATTTCAGTTGCCAGTTGGT‑TAAACTTACGTGCTGGTGCGGCCAAACCAACGGTATCAATGCTGGGGAAAGTGTTCAGCATTGTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATGTTTTGCCAGGAGATTAAAAGTATGGGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGaa < 1:47044/221‑1 (MQ=255) cacaACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTg > 1:522146/1‑219 (MQ=255) cagaCGGTGTGGTCGATCTGAGTTGCAAGTTGGT‑AAAACTTACGTTCTGAGGCTGCGCAACCACCGGTAACAATACTCGGGAAAATGTTCAGCACTTCGAGTTTACCCGCAAACTGCCCGAGGGTGACGTCAGACAGATCTTTTGCCAGGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGaa < 2:57494/223‑1 (MQ=255) caaagggTTTTGTCGATCTCA‑TTGGCCGTGGTTGTAACCTTCCGTACTGTTGCGGCGCAAACACCGGTACCAATACCGGGGAAAATGTTCAGCATGTTGCTTTTCCCCGCAAACTGCCCGAGGGTGACGCCAGACGGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTCCCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAAc < 2:750894/222‑1 (MQ=255) cGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAAAGTAAAAGACTGCGCTTTCTTACCCGCCTGCGGGTTGGAATTGGGAACTGTACCCGGGTTGCCCTGGAATTGAACGGTTtg > 1:82577/1‑229 (MQ=255) gtttggtcatttcttgTTGCCTGTTGGT‑TAAAATTCGTTCCTGTCGCGGCGCAAACCCCGGTATCAATACCGGGAAAAATTTTCAGCCTTTTGCGTTTCCCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTGGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTtgtg < 2:93283/214‑1 (MQ=255) tgttgtCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGGGACTGTACCCGGGTTGCCCTGGAAATGAAAGGTTggt > 2:411486/1‑227 (MQ=255) ttgtCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGCAGGAATTGGCTACTGTAACCGGGTTGCCCTGCAAATGAACGGgttaggca > 2:55722/1‑223 (MQ=255) ttgtCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATAAACGGTTtgtgcaa > 1:299866/1‑226 (MQ=255) ttgtCGATATCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCTGCCAGATCTTTTGCCACGAGGGAAACAGTCTGCGCTTTGGTAACCGCCTGCGGGCTGGAATTGGCGCATGTAACCGGGTTTCCCTGGAAATGACCgggaggggaag > 2:360300/1‑220 (MQ=255) ggcgatccaggTTGACTGTTGGT‑TAAATTTCTGTCCTGTTGCGCGGAAAACCCCGTAAACAAAACTCGGGAAAATGTCCACCCCTTTGCTTTTACCCGAAACCTGACGGAGGTGGACGTCAGACAGAGTTTTTGCCACGATAGTAAAAGTCTGCGTTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATg < 2:734872/219‑1 (MQ=255) tCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAACCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGGGCTTTGCTACCCGCCTGCGGGATGGATTTGGCTACGGTACACGGGTTGCCCTGGAAATAAACGGTTTGGGACAt > 1:493432/1‑227 (MQ=255) tCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACGCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGAcct > 1:393570/1‑225 (MQ=255) ctcGGTGGCCGGTTGTT‑TAACTTTACGTTCGGATGGGGCCAAAACACGGGTATAAATCCCGGGAAAAATGTTCAGCTCTTTGCGTTTCCCCGAAAACTGACCGAGGGTGACGTCAGACCGATCTTTTGCCACGAGAGAAAAAGTCTGCGCTTTGCTCCCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTAt < 2:67591/228‑1 (MQ=255) ctcAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTtgtg > 1:93283/1‑218 (MQ=255) ctgtgtgT‑TAAACTGACGTATGGGTGGGGCGAAAACCCCGGTATCAAAACTGGGGAAAATGTTCAGCACTTTGCGTTTACCCGAAAACTGACGGAGGGTGACGTCAGACAGATCTTTGGCCAAGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGtt < 2:88874/223‑1 (MQ=255) aGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGttt > 1:513516/1‑229 (MQ=255) ttgtgtaacgttACGCACGTATGCGGCGAAAACCCCGGTATCAATACGCGGGAAAATGTTCATCACTTTCGTTTTACCCGCAAACTGACCGAGGGTGCCGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTAc < 1:385820/219‑1 (MQ=255) tGGT‑TAAACTTACGTCCTGATGCGGCCAAACCCCCGGTATCAATACGCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAATGGCCCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACa < 1:268397/229‑1 (MQ=255) t‑tAAACTTACGTATTGATGCGGCCCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCCCTTTGCTTTTGCCCGCAACCGGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACa < 1:713946/226‑1 (MQ=255) aatttACGTATCGGTGCGGCCAAAACACCGGGAAAATAACTGGGGAAAGTGTCAAGCATTTTGCGTTTCCCCGAAAACGGACCGGGGGTCACGTCAGACAGATTTTTTGCCAGGAGGGAAAAAGTCGGCGCTTTGCTACCCGGCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGt < 2:735518/226‑1 (MQ=255) tGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGAAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCacac < 1:376053/229‑1 (MQ=255) ggagccggcgCAAACCCCGGTATCAATCCTCGAGAAAATGTTCAGCCCTTTGCGTTTCCCCGCAAACGGACCGGGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCacac < 1:503633/224‑1 (MQ=255) aTGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGACTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAAGGAACGGTTTGTACCATGCTTAACTTCCCGGTTTACATATAGTTAACGTCACACCt > 2:632638/1‑229 (MQ=255) ggccCAAACCCGGGTATAAATCCTCGGGAAATTGTTCAGCATTTTGCGTTTCCCGGCAAACTGACCGGGGGTGCAGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGtt < 2:29115/229‑1 (MQ=255) cacCGTTAACAATACTTGGGAAAATTGTCAGGACTTGGCGTTTACACGCAAACTGACCGAGGGTGTCGACAGACAGAACTTTTGCCACGAGAGTAAAAGTCTGCGATTTGGTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTaa < 1:558407/229‑1 (MQ=255) taaaaaTACT‑GGGAAAATGTTCAGCCTTTTGCGTTTACCCGAAACCGGACCGAGGGTGACGTCAGACAGATCTTTTGCCGCGGGAGTAAAAGTCTGCGTTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCaa < 1:226936/225‑1 (MQ=255) cGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGc > 2:529085/1‑229 (MQ=255) cGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATATTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCT‑ACTGTCAATAACACAGCa > 1:635050/1‑229 (MQ=255) gggAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTCGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTACCCTGGAACTGAACGGTTTGAGACATGATTATCTTTCCTGTTTCCCTATAGTTAACGTCACACCTAGATTATGCTAACTGTCACTAACACAGCa > 1:33659/1‑229 (MQ=255) gggAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGAGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGGGACATGATTATCTTTCCGGTTTACATATAGTTAACGTCGCCCCTAGTTTATGCTAACTGTCAATAACACACCa > 2:237453/1‑229 (MQ=255) gggAAAATGTTAAGACTTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCa < 1:282712/229‑1 (MQ=255) gggAAAAGTTTCAGCCCGTTGCGTTTACCCGAAAACGGACCGAGGGGGACGCTAGTCAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCa < 1:393397/229‑1 (MQ=255) ggAATATGGTCAGCTCTTTGCGTTTACCCGGAAACTGACCGGGGTTGACGGCGGACAGTTCTTTTGCCACGAGAGTAAAAGTCTGCTCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCa < 1:730364/228‑1 (MQ=255) gAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACG‑GAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGACCGGTTTGTGAAATGATTATCTTTCCTGTTTACTTATAGTTAAAGGCACACCTAGTTTTTGCTAACTGTCAATAACACATCAAAc > 2:365574/1‑229 (MQ=255) aaTGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACAAATAGTTAACGTCACACCGAGTTTATGCTACCTGTCAATAACACAGCAAACGc > 1:515148/1‑229 (MQ=255) | TTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGT‑TAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGC > NC_000913/1388448‑1388884 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |