Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,388,666 A→C 16.9% V49G (GTG→GGG)  tpx ← lipid hydroperoxide peroxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,388,6660AC16.9% 143.5 / 2.3 68V49G (GTG→GGG) tpxlipid hydroperoxide peroxidase
Reads supporting (aligned to +/- strand):  ref base A (31/22);  new base C (2/11);  total (33/35)
Fisher's exact test for biased strand distribution p-value = 1.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGC  >  NC_000913/1388448‑1388884
                                                                                                                                                                                                                           |                                                                                                                                                                                                                          
ttGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGtt                                                                                                                                                                                                                   <  1:590809/228‑1 (MQ=255)
gtGCAACACCTGAAGCTTGCAGAAATTCGGCGGTACGGAAAGGGGAGAGGGTGATAACGTTGTTCAGACCGTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGtt                                                                                                                                                                                                                   <  1:666683/227‑1 (MQ=255)
         cGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCATCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCaaa                                                                                                                                                                                                         <  1:623686/229‑1 (MQ=255)
         cGTAAGAGTGCAGAAAATCAGCGTGACGGAATGTGGAGAGGGTGATAACTGTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGGGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCaaa                                                                                                                                                                                                         <  1:345926/229‑1 (MQ=255)
                 tGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAACAACCGGTATCAATACTCGGGAAAATGTTCAGCCCTTTTCGTTTACCCGCAAAcgtcccgc                                                                                                                                                                                                 >  1:549183/1‑222 (MQ=255)
                 tGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCACACCTTTGAGTTTACCCCCAAACTGACCGa                                                                                                                                                                                                 >  2:713945/1‑229 (MQ=255)
                 tGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGAGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACGGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATTGTCATAACTTTGCGTTTACCCCAAAACTGAccag                                                                                                                                                                                                 >  2:586160/1‑227 (MQ=255)
                   cAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCGCCAAACTGACCGAgg                                                                                                                                                                                               >  1:88874/1‑229 (MQ=255)
                                             agagGGTGATAACGTTTTTCAGACCTTCCGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGGGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCAc                                                                                                                                                                     <  1:77815/229‑1 (MQ=255)
                                             agacgtttaTAACCGTGTCCAGGCATTCTGCGACGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGTACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTGCTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCCATTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCAc                                                                                                                                                                     <  1:327284/221‑1 (MQ=255)
                                                gggTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACga                                                                                                                                                                   <  1:130875/228‑1 (MQ=255)
                                                          cttcttCAGCCCTTATCCGCCCGAGAAACGGGGCTGGGGGAACGGCAGATCGGCAGAGATACACAGCCCAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGt                                                                                                                                                         <  2:702008/224‑1 (MQ=255)
                                                            tgttCAGACCTTCTGCGCCCCAGAAACGAGTCGGGGCGAACGGCAGATCGGCAAAGTTACACAGCACAACGGTGTTGGCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGGTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCt                                                                                                                                                       <  2:745061/228‑1 (MQ=255)
                                                             gttCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTCCGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGAAAGATCTTTTGCCACGAGAGAAAAAATCTg                                                                                                                                                      >  2:607877/1‑228 (MQ=255)
                                                             gttCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTg                                                                                                                                                      <  1:607878/228‑1 (MQ=255)
                                                             gttCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTAAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGGGACGTCAGACAGATCTTTTGCCACGAGAGTCAAAGTCTg                                                                                                                                                      >  1:254270/1‑228 (MQ=255)
                                                                   aCCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAACCTGACCAGGGGTGAGTTCAGACAGATCTTTTGCAACGAGAGTAAAAGAATGCGCttgg                                                                                                                                               >  2:630208/1‑227 (MQ=255)
                                                                   aCCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGAAAGATCTTTTGCCACGAGAGTAAAAATCTGCGCt                                                                                                                                                  >  1:510315/1‑226 (MQ=255)
                                                                        cTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCCGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTAcc                                                                                                                                          >  1:29115/1‑229 (MQ=255)
                                                                        cTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGAACTTTTGCCACGAGAGTAAAAGCCTCGGCTTTGCTAcc                                                                                                                                          >  2:376053/1‑229 (MQ=255)
                                                                        cTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGGATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAggg                                                                                                                                                                                              >  2:287284/1‑177 (MQ=255)
                                                                        cTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGAGTTGTAGATCTCAGATGCCCGTTGGGTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTTCGTTTAACCGCAACATGACCAAGGCAGACGTCAGACAAAGCTTTTGCCACTGCACTAAAAGACTTGACTTTGCTAcc                                                                                                                                          >  1:351499/1‑229 (MQ=255)
                                                                               gcAGAAACGACGCTGGGCGAACGGCAGATCGGCAGGGATACACAGCAAAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGGGGCCCAAACCCCGGTATCAATCCTGGGGAAAATGTTCAGCCTTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCAAGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTg                                                                                                                                    <  2:254270/228‑1 (MQ=255)
                                                                                cAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGGGACGTCAGCCACATCTTTTGCCACGAGAGTAAAAGCCTGGGCTTTGCAACCCACCTggg                                                                                                                                  >  2:282712/1‑227 (MQ=255)
                                                                                   aaccggaaccgggCGAACGGCAGTTCGGCAGAGATACACAGCACAACGGTTTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACGTATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATTTTCAGCCCTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGa                                                                                                                               <  1:178664/219‑1 (MQ=255)
                                                                                             gggCGAACGGCAGATCGGCAGAGATACCCAGACCAACGGTTTTGCCGATCTCAGTTGCCAGTTGGTTAACCTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAACCTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTCCCCGCCTGCGGGATGGAATTGGc                                                                                                                     <  1:586161/229‑1 (MQ=255)
                                                                                             gggCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGGAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGc                                                                                                                     >  1:683239/1‑229 (MQ=255)
                                                                                               gCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTTCCACGAGAGTAAAAACATGCGCTTTGCTACCCGCCCGCGGGATGGAAATGGCGa                                                                                                                   >  1:751653/1‑229 (MQ=255)
                                                                                                                agtgttaccaacgcacaCGGTTGTGTCCACCTCGTTTGCCAGTTGGTTAAATTTCCGTACTGATGCGGCGCAAACCCCGGTATCAAGACCCGGGAAAATGTTCAGCCTTTTGCGTTTACCCGCAAACGGACCGAGGGTGACGTCAGACAGATCTTTTGCCAGGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGccc                                                                                                  <  2:751652/213‑1 (MQ=255)
                                                                                                                  aattaccaaGCAAAACGGTGGTGTCAGTCTCAGTTGTCATTTGGTTAAACTTACGGACTGATGCGGCGCAAACACCGGTACAAATACTCGGAAAATTGTTCAGCACTTTGCGTTTCCCCGCAAACTGACCGGGGGTGCCGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTg                                                                                                <  2:522146/221‑1 (MQ=255)
                                                                                                                    gtccaactcccaccGGTGTTGTCGATCCAAGTTTCAAGTTGGTTAAACTTACGTCCGGATGCGGCGCACACCCCGGTATCAATACGCGGGAAAATGTTCAGCACTTTGCGTTTCCCCGCAAATTGACCGAGGGTAACGTCAGACAGATTTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGa                                                                                              <  2:618806/216‑1 (MQ=255)
                                                                                                                         caGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACCGATCTTTTGCCACGAGAGTAAAAGTCGGCGCTTTGCTACCCGCCTGCGGGTTGGAATTGGCGACTGTAACCGGGTTGCCCGGGAAAAGa                                                                                         >  2:370693/1‑229 (MQ=255)
                                                                                                                           ggagacgggtggtGGCGATTTCAGTTGCCAGTTGGTTAAACTTACGTGCTGGTGCGGCCAAACCAACGGTATCAATGCTGGGGAAAGTGTTCAGCATTGTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATGTTTTGCCAGGAGATTAAAAGTATGGGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGaa                                                                                        <  1:47044/221‑1 (MQ=255)
                                                                                                                            cacaACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTg                                                                                                >  1:522146/1‑219 (MQ=255)
                                                                                                                             cagaCGGTGTGGTCGATCTGAGTTGCAAGTTGGTAAAACTTACGTTCTGAGGCTGCGCAACCACCGGTAACAATACTCGGGAAAATGTTCAGCACTTCGAGTTTACCCGCAAACTGCCCGAGGGTGACGTCAGACAGATCTTTTGCCAGGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGaa                                                                                        <  2:57494/223‑1 (MQ=255)
                                                                                                                             caaagggTTTTGTCGATCTCATTGGCCGTGGTTGTAACCTTCCGTACTGTTGCGGCGCAAACACCGGTACCAATACCGGGGAAAATGTTCAGCATGTTGCTTTTCCCCGCAAACTGCCCGAGGGTGACGCCAGACGGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTCCCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAAc                                                                                       <  2:750894/222‑1 (MQ=255)
                                                                                                                                 cGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAAAGTAAAAGACTGCGCTTTCTTACCCGCCTGCGGGTTGGAATTGGGAACTGTACCCGGGTTGCCCTGGAATTGAACGGTTtg                                                                                 >  1:82577/1‑229 (MQ=255)
                                                                                                                                   gtttggtcatttcttgTTGCCTGTTGGTTAAAATTCGTTCCTGTCGCGGCGCAAACCCCGGTATCAATACCGGGAAAAATTTTCAGCCTTTTGCGTTTCCCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTGGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTtgtg                                                                               <  2:93283/214‑1 (MQ=255)
                                                                                                                                    tgttgtCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGGGACTGTACCCGGGTTGCCCTGGAAATGAAAGGTTggt                                                                                >  2:411486/1‑227 (MQ=255)
                                                                                                                                      ttgtCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGCAGGAATTGGCTACTGTAACCGGGTTGCCCTGCAAATGAACGGgttaggca                                                                             >  2:55722/1‑223 (MQ=255)
                                                                                                                                      ttgtCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATAAACGGTTtgtgcaa                                                                            >  1:299866/1‑226 (MQ=255)
                                                                                                                                      ttgtCGATATCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCTGCCAGATCTTTTGCCACGAGGGAAACAGTCTGCGCTTTGGTAACCGCCTGCGGGCTGGAATTGGCGCATGTAACCGGGTTTCCCTGGAAATGACCgggaggggaag                                                                            >  2:360300/1‑220 (MQ=255)
                                                                                                                                        ggcgatccaggTTGACTGTTGGTTAAATTTCTGTCCTGTTGCGCGGAAAACCCCGTAAACAAAACTCGGGAAAATGTCCACCCCTTTGCTTTTACCCGAAACCTGACGGAGGTGGACGTCAGACAGAGTTTTTGCCACGATAGTAAAAGTCTGCGTTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATg                                                                          <  2:734872/219‑1 (MQ=255)
                                                                                                                                         tCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAACCACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGGGCTTTGCTACCCGCCTGCGGGATGGATTTGGCTACGGTACACGGGTTGCCCTGGAAATAAACGGTTTGGGACAt                                                                           >  1:493432/1‑227 (MQ=255)
                                                                                                                                         tCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACGCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGAcct                                                                           >  1:393570/1‑225 (MQ=255)
                                                                                                                                              ctcGGTGGCCGGTTGTTTAACTTTACGTTCGGATGGGGCCAAAACACGGGTATAAATCCCGGGAAAAATGTTCAGCTCTTTGCGTTTCCCCGAAAACTGACCGAGGGTGACGTCAGACCGATCTTTTGCCACGAGAGAAAAAGTCTGCGCTTTGCTCCCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTAt                                                                     <  2:67591/228‑1 (MQ=255)
                                                                                                                                              ctcAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTtgtg                                                                               >  1:93283/1‑218 (MQ=255)
                                                                                                                                                       ctgtgtgTTAAACTGACGTATGGGTGGGGCGAAAACCCCGGTATCAAAACTGGGGAAAATGTTCAGCACTTTGCGTTTACCCGAAAACTGACGGAGGGTGACGTCAGACAGATCTTTGGCCAAGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGtt                                                           <  2:88874/223‑1 (MQ=255)
                                                                                                                                                        aGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGttt                                                          >  1:513516/1‑229 (MQ=255)
                                                                                                                                                           ttgtgtaacgttACGCACGTATGCGGCGAAAACCCCGGTATCAATACGCGGGAAAATGTTCATCACTTTCGTTTTACCCGCAAACTGACCGAGGGTGCCGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTAc                                                        <  1:385820/219‑1 (MQ=255)
                                                                                                                                                           tGGTTAAACTTACGTCCTGATGCGGCCAAACCCCCGGTATCAATACGCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAATGGCCCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACa                                                       <  1:268397/229‑1 (MQ=255)
                                                                                                                                                              ttAAACTTACGTATTGATGCGGCCCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCCCTTTGCTTTTGCCCGCAACCGGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACa                                                       <  1:713946/226‑1 (MQ=255)
                                                                                                                                                                  aatttACGTATCGGTGCGGCCAAAACACCGGGAAAATAACTGGGGAAAGTGTCAAGCATTTTGCGTTTCCCCGAAAACGGACCGGGGGTCACGTCAGACAGATTTTTTGCCAGGAGGGAAAAAGTCGGCGCTTTGCTACCCGGCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGt                                                 <  2:735518/226‑1 (MQ=255)
                                                                                                                                                                             tGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGAAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCacac                                      <  1:376053/229‑1 (MQ=255)
                                                                                                                                                                             ggagccggcgCAAACCCCGGTATCAATCCTCGAGAAAATGTTCAGCCCTTTGCGTTTCCCCGCAAACGGACCGGGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCacac                                      <  1:503633/224‑1 (MQ=255)
                                                                                                                                                                               aTGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGACTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAAGGAACGGTTTGTACCATGCTTAACTTCCCGGTTTACATATAGTTAACGTCACACCt                                    >  2:632638/1‑229 (MQ=255)
                                                                                                                                                                                   ggccCAAACCCGGGTATAAATCCTCGGGAAATTGTTCAGCATTTTGCGTTTCCCGGCAAACTGACCGGGGGTGCAGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGtt                                <  2:29115/229‑1 (MQ=255)
                                                                                                                                                                                           cacCGTTAACAATACTTGGGAAAATTGTCAGGACTTGGCGTTTACACGCAAACTGACCGAGGGTGTCGACAGACAGAACTTTTGCCACGAGAGTAAAAGTCTGCGATTTGGTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTaa                        <  1:558407/229‑1 (MQ=255)
                                                                                                                                                                                                 taaaaaTACTGGGAAAATGTTCAGCCTTTTGCGTTTACCCGAAACCGGACCGAGGGTGACGTCAGACAGATCTTTTGCCGCGGGAGTAAAAGTCTGCGTTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCaa                 <  1:226936/225‑1 (MQ=255)
                                                                                                                                                                                                           cGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGc        >  2:529085/1‑229 (MQ=255)
                                                                                                                                                                                                           cGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATATTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTACTGTCAATAACACAGCa       >  1:635050/1‑229 (MQ=255)
                                                                                                                                                                                                            gggAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTCGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTACCCTGGAACTGAACGGTTTGAGACATGATTATCTTTCCTGTTTCCCTATAGTTAACGTCACACCTAGATTATGCTAACTGTCACTAACACAGCa       >  1:33659/1‑229 (MQ=255)
                                                                                                                                                                                                            gggAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGAGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGGGACATGATTATCTTTCCGGTTTACATATAGTTAACGTCGCCCCTAGTTTATGCTAACTGTCAATAACACACCa       >  2:237453/1‑229 (MQ=255)
                                                                                                                                                                                                            gggAAAATGTTAAGACTTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCa       <  1:282712/229‑1 (MQ=255)
                                                                                                                                                                                                            gggAAAAGTTTCAGCCCGTTGCGTTTACCCGAAAACGGACCGAGGGGGACGCTAGTCAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCa       <  1:393397/229‑1 (MQ=255)
                                                                                                                                                                                                             ggAATATGGTCAGCTCTTTGCGTTTACCCGGAAACTGACCGGGGTTGACGGCGGACAGTTCTTTTGCCACGAGAGTAAAAGTCTGCTCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCa       <  1:730364/228‑1 (MQ=255)
                                                                                                                                                                                                              gAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGACCGGTTTGTGAAATGATTATCTTTCCTGTTTACTTATAGTTAAAGGCACACCTAGTTTTTGCTAACTGTCAATAACACATCAAAc    >  2:365574/1‑229 (MQ=255)
                                                                                                                                                                                                                 aaTGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACAAATAGTTAACGTCACACCGAGTTTATGCTACCTGTCAATAACACAGCAAACGc  >  1:515148/1‑229 (MQ=255)
                                                                                                                                                                                                                           |                                                                                                                                                                                                                          
TTGCCACACCGTAAGCTTGCAGAAATTCAGCGTTACGGAAAGTGGAGAGGGTGATAACGTTGTTCAGACCTTCTGCGCCGCAGAAACGAGACTGGGCGAACGGCAGATCGGCAGAGATACACAGCACAACGGTGTTGTCGATCTCAGTTGCCAGTTGGTTAAACTTACGTACTGATGCGGCGCAAACACCGGTATCAATACTCGGGAAAATGTTCAGCACTTTGCGTTTACCCGCAAACTGACCGAGGGTGACGTCAGACAGATCTTTTGCCACGAGAGTAAAAGTCTGCGCTTTGCTACCCGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGC  >  NC_000913/1388448‑1388884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: