Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,695,755 T→C 12.7% E106G (GAG→GGG)  uidA ← beta‑D‑glucuronidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,695,7550TC12.7% 155.6 / 2.4 70E106G (GAG→GGG) uidAbeta‑D‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base T (29/28);  new base C (5/5);  total (34/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCTTTCCCACCAACGCTGAT  >  NC_000913/1695531‑1695980
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  
tcggctgggcttaaagCATTACGTTGCGATTGATCCCGGCATAGTTAAAGAAAGCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCAt                                                                                                                                                                                                                                <  1:304838/215‑1 (MQ=255)
        gtgtAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGCCTGCTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCt                                                                                                                                                                                                                        <  1:126412/229‑1 (MQ=255)
             tagttttttgCTGTGGTGGATCCTGGCATAGTTAAAGAAATCATGGAATTAAGGCTGCTTTTTCTGGCCGTTTT‑CGTCGGTAATCACCATTCCCGGGGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGatta                                                                                                                                                                                                                   <  2:283073/220‑1 (MQ=255)
                   tACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCCGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCACTAACATACGGCGTGCCATCGGCTTCCAATGCCGAATAGCCGCCCCGAGTCCCCCCCACTTCCTCTTTATTTAc                                                                                                                                                                                                              >  2:707860/1‑228 (MQ=255)
                   tACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCCGTTCGTTGTTCACAA‑CAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACCGCCGGACATCGGATTCAAAAGGGGTTAAGCCGCCCCTCTGCCCCATCACTTCCCGCTTAATGAc                                                                                                                                                                                                              >  2:730647/1‑228 (MQ=255)
                   tACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGTGGATACG‑GACACTTTTCCCGGCAATAACATAAGGAGTGACATCGGCTTCAAATGGCGGATAGCCGCCCTGATCCTCCATCACTTCCTGTTTATTGAc                                                                                                                                                                                                              >  1:413077/1‑228 (MQ=255)
                   tACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGAc                                                                                                                                                                                                              >  1:160731/1‑228 (MQ=255)
                   tACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTAATGGTCCATCAATTCCTGATTATTGAc                                                                                                                                                                                                              >  2:47200/1‑228 (MQ=255)
                    aCGCTGCGATGCATCCCGGACTAGTTAAAGAAATCCTGGAAGTAAGACTGCTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGTCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGAc                                                                                                                                                                                                              <  1:730648/227‑1 (MQ=255)
                     cGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGATTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAAAGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCCGCTGTCCCATCCATTCCCGATTCTTGACCca                                                                                                                                                                                                           >  1:270153/1‑229 (MQ=255)
                           gATGGATCCCGGGATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACt                                                                                                                                                                                                       <  1:411705/227‑1 (MQ=255)
                           gATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGCCTGGTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGTTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGatta                                                                                                                                                                                                                   <  1:720471/215‑1 (MQ=255)
                           gATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATATGCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCAGGACCTCGGCTTCAAATGGCTTATAGCCGCCCTGATGCCCCATAACTTCCTGCTTATGAAccccacccc                                                                                                                                                                                                      >  2:720470/1‑222 (MQ=255)
                             ttgATCCCGGGATATGTAAAAGAATCATGGAAGGAAGTCTGTTTTGTCTGTGCGTGTT‑CTGCGGTAATCACCATTCCCGGGGGGATAGTCTGCCAGTTCCGTTCGTTGTTCACAC‑AAACGTGTATACG‑TACCCTTTTCCCGGCAATAACATACCGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCGCCATCACTTCCTGATTATTGACCCACACTTTGc                                                                                                                                                                                                   <  2:594930/227‑1 (MQ=255)
                                    cGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCCAATGGCGTATAACCGCCCTGATGCTCCATCACTTCCTGATTATTGAACCACCCTTTGACGTact                                                                                                                                                                                             >  1:632925/1‑226 (MQ=255)
                                       aataagtAAAGAATGCATGGAAGTAAATCTGGTTTGTCTTGCCGTTTT‑CCTCCGTAACCACCATTCCCGGCGGGATATTCTGCCCGTTCCGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACCCTTTTCCCGGCAATAACATTCGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGt                                                                                                                                                                                         <  2:632924/223‑1 (MQ=255)
                                                      ctttgaactcagccgtcTTTTTTTGTCCGTTTTGCGT‑GGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AACCGGTGGTACG‑TCCCCTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACg                                                                                                                                                                          <  2:91685/213‑1 (MQ=255)
                                                             tttagccTGCTTTTTTTTGCCGTTTT‑CTTCGGTAATTCCCATTCCCGGTGTGATAGTCTGCCAGTTCAGTTCGTGGTTCACAC‑AAACGGTGATACG‑TACGCTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACg                                                                                                                                                                   <  2:349673/223‑1 (MQ=255)
                                                                      cTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGCTGCTCCATCACTTCCTGATTATTTACCCACACCTTTCCGTAATGAAGGACCCCACCGAAAccaacaacaaaaacctg                                                                                                                                                           >  2:100010/1‑212 (MQ=255)
                                                                       cttttttTGCCTGTTT‑TGTCGGTATACCCCATTCCCGGGCGGATAGTCTGCCCGTTCAGTTCGTTGTTCCCAC‑AAACGGTGCTACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCAGATTATTGACCCACACTTTGCCGTAATGAGTGTCCGCATCGAAACCCAGCACGATACGCTgg                                                                                                                                                          <  2:196837/227‑1 (MQ=255)
                                                                        ggggatgtgaataagagacaggTAATCCCACTTCCCGGCGGGATAGTCTGCCAGTTCAGTTCTTTGTTCACAC‑AAACGGTTATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGc                                                                                                                                                         <  1:736282/209‑1 (MQ=255)
                                                                                ccGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCCCTTCCTGATTATTTACCCACACATTTCCGTCATTAGTGACCGCAGCGAAACGCAGCAACAAACCCTGCCCCCCCCAAc                                                                                                                                                >  2:97182/1‑229 (MQ=255)
                                                                                     ttccgtcgGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCCCCC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACTTCGGGTTCAAATGGCGTATAGCCGCCCTGATTCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCttt                                                                                                                                            <  1:53836/226‑1 (MQ=255)
                                                                                     tt‑cttcGGTATTTCCCATTCCCGGGGGTATAGCTTGCCCTTCCAGTTCGTT‑TTCCCACAAAACGGTGATCCG‑TAACCTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTAAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTc                                                                                                                                           <  2:383836/229‑1 (MQ=255)
                                                                                     tt‑CGTCGGTAATCCCACTTCCCGGCGGGTTATCCTTCCCACTCAGTTCGTTTTTCACAC‑AAACGGTGATCCC‑TACCCGTTTCCCGCCAATAAGAGACGGCTTGACATCGGTTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTc                                                                                                                                           <  2:551787/229‑1 (MQ=255)
                                                                                     tt‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACATTTTTCCCGGCAATAACATCCGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTGCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTc                                                                                                                                           <  1:457624/229‑1 (MQ=255)
                                                                                     tt‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTc                                                                                                                                           <  1:47200/229‑1 (MQ=255)
                                                                                                   aCCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCCCTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACtt                                                                                                                              <  1:673219/229‑1 (MQ=255)
                                                                                                        tCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCCCACTTTGCCGTAATGAATGACCGCATCGAAACGAGACACCTAACGCCGGCCTGCCCAACCTTTCGGTATaaaagacacacg                                                                                                                          >  2:73354/1‑219 (MQ=255)
                                                                                                        tCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCCTGCCTGCCCAACCTTTCGGAATAAAGACTTcgcg                                                                                                                          >  1:436595/1‑228 (MQ=255)
                                                                                                        tCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGCCGTATAGCCGCCCTGATGCTCCCTCACTTCCTGATTATTTCCCCACCCTTTTCCGTAATGAGGGACCGCATCGAAACGCACCCCGATCCCCCTGCCTGCCCCAcccccgtcccttaaaacacactaa                                                                                                                         >  2:736281/1‑207 (MQ=255)
                                                                                                        tCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAAGAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTcgcg                                                                                                                          <  1:97182/228‑1 (MQ=255)
                                                                                                            ggcggGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGGATAAAGACTTCACGCTGa                                                                                                                      >  1:257726/1‑228 (MQ=255)
                                                                                                             gcggGATAGTCTGCCAGGTCAGTTCGTTGTTCACAC‑AAACGGGGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCCCCATCACTTCCTGCTTATTGACCCACCCTTTGCCGTAATGAGTGACCGCCACGAAACGCAGCACGATACGCTGGCCTCCCCAACCTTGCGTTATAAAAACATCCCGCTgca                                                                                                                     >  2:56142/1‑226 (MQ=255)
                                                                                                                          ccAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGAAACCAGCCGTTGccc                                                                                                       >  1:112656/1‑229 (MQ=255)
                                                                                                                           cagttcagttCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGGATAAAGACTTCGCGCTGATACCAGACGTTGCCCg                                                                                                      >  1:176810/1‑229 (MQ=255)
                                                                                                                           cagttcagttCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATAGCAACGACGCACGATACGCGTGCCCGGCCAACCTTTCGGTATAAAGCCATCGCGCAGATACCCGACGATccccc                                                                                                      >  2:479057/1‑228 (MQ=255)
                                                                                                                                cagttcGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCAGCTTCAAATGGCGTATACCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTTACCGCATCGAAACGCAGCCCCATACGCTGGCCTGACCACCCTTTCGATATAAAAACTTCACCCTGGTAcccacaagtgcccccaca                                                                                                  >  2:646339/1‑212 (MQ=255)
                                                                                                                                     cgttcgtCACAC‑AAACGGTGATACG‑TACACTTGTACCGGCAATAACAGACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCCCACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCTGTATAAAGACTTCGCGCTGATACCAGACGTGGCCCGCATAATT‑ACg                                                                                           <  1:480152/223‑1 (MQ=255)
                                                                                                                                     ccgtgttCACAC‑AAAACGTTATCCGTTCCATTTGCCCCGACAAT‑ACCTGCGGCGTGACCTCGGCTTCAAATGGCGTAGAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACg                                                                                           <  1:36507/226‑1 (MQ=255)
                                                                                                                                     cGTTGTTCACAC‑AAACGGTGATACG‑TTCACTTTTCCCCGCAATAACATTCGGCGTGACAGCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACg                                                                                           <  1:110121/229‑1 (MQ=255)
                                                                                                                                     cGTTGTGCTCAC‑CAACGTGGATACG‑TACCAGTTTCCCGCAAATAACATACGGCGTGTCATCGGATTCAAATGGCGTATAGCCGCCCTGATGCCCCATCACTGCCTTATTATTGACCCACACTCTGCCGTACTGAGTGACTGCAACGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACg                                                                                           <  2:432125/229‑1 (MQ=255)
                                                                                                                                                          aTACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGCTACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAAAATCtcaacaggggaact                                                                       >  2:158046/1‑216 (MQ=255)
                                                                                                                                                          aTACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAAAATCTGCATCGGCGAACt                                                                       >  1:222288/1‑229 (MQ=255)
                                                                                                                                                           ttcG‑TACACTTTTCCCGGCAATAACATCCGGTCTGACATCGGTTTTAAATGGCGTATAGCCGCCCTGATGCTCCATCCCTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTg                                                                      <  1:355312/227‑1 (MQ=255)
                                                                                                                                                            ttgttgcacTTTTCCCTGCAATACCATGCGGCGTGACATCGGCTTCAAATGGCGTATGGCCGCCCGGATGCGCCATCACTTCCTGATTATTGACCCACACTTTGCCGTACTGACTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCTGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTg                                                                      <  2:270153/223‑1 (MQ=255)
                                                                                                                                                            gcG‑TACACTTTTCCCGGCAATAACATTCGGCGTGAAATCGGCTTCAAATGGCGTATAGCCGGCCTGATGCCCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTg                                                                      <  1:345787/227‑1 (MQ=255)
                                                                                                                                                               gttctcTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTGCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATc                                                                   <  1:517955/224‑1 (MQ=255)
                                                                                                                                                                 acacTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGCAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAAACCTCGCGCTGATACCCGACGTTGCCCCCCAAATT‑TCAAAAATCttaaccccgcaactaatcg                                                                  >  2:345787/1‑210 (MQ=255)
                                                                                                                                                                   acTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTCTAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACTATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGACCGCATAATT‑CCGCATATCTGCATCGGCGCACTGACCGTTa                                                               >  1:687408/1‑229 (MQ=255)
                                                                                                                                                                       ttCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTTACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGAGACCAGACGGTGCCCGCATAATT‑ACGAATATCTGCATCCGCGACCTGAACGTTAAAAc                                                           >  2:735457/1‑229 (MQ=255)
                                                                                                                                                                       ttCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTGGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGCATATCTTAATCGGCGTACTGACCGTTaaaa                                                            >  1:440028/1‑228 (MQ=255)
                                                                                                                                                                          ccGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCAAACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGAATAAAACATTCCCGCTGATACCAGACGTTGCCCGCATAATT‑ACGCAAATCTGCATCGCCGCAATGAACGTTAAAAcgac                                                        >  2:4202/1‑226 (MQ=255)
                                                                                                                                                                                 tAACATACGGCGTTACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCCCTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGcac                                                 <  1:707861/229‑1 (MQ=255)
                                                                                                                                                                                           ggTGACCTCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGcc                                       <  2:590565/228‑1 (MQ=255)
                                                                                                                                                                                           cGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCCTCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCCTAATT‑ACGAACAACTGCCTCGGCGAACCGAACCTTAAAAAGGCCAGGCACACCAAATGcc                                       >  2:352443/1‑229 (MQ=255)
                                                                                                                                                                                           cGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTc                                                                                                                                           >  1:227298/1‑130 (MQ=255)
                                                                                                                                                                                           cGTGACATCGGCATCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTc                                                                                                                                           <  2:227298/130‑1 (MQ=255)
                                                                                                                                                                                             tGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGCCGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGACCTGCTCTGTAAACCTGCCTGGCACAGCAATTGCCCg                                     >  1:484763/1‑229 (MQ=255)
                                                                                                                                                                                                cATCGGCTTCAAATGGCGTATAGCCCCCCTGATGCCCCATCCCTTCCTGGTTATTGACCCACCCTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCt                                  <  2:160731/229‑1 (MQ=255)
                                                                                                                                                                                                     gCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCg                                                                  <  1:186853/192‑1 (MQ=255)
                                                                                                                                                                                                     gCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATATGCATCGGCGAACTGATCg                                                                  >  2:186853/1‑192 (MQ=255)
                                                                                                                                                                                                     gCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGAGCTGATACCAGACCTTTCCCGCCTAATT‑ACGAATATATGCATCGGCGAACTGATCGTTAAAACCCCCTGGCAAAACAATTGCCCGGCcttctt                             >  2:568244/1‑229 (MQ=255)
                                                                                                                                                                                                              tgcttttaGCCGCCCTTGTTCCCCATCCCCTCCGGATTATTGACCCACACTTTGCCGGAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACgcgc                    <  2:257726/223‑1 (MQ=255)
                                                                                                                                                                                                                 ctattGCCGCCCTTATGCCCCCTCCCTTCCTGATTATTGACCCCCCCTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATTCGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCttt                 <  2:176810/228‑1 (MQ=255)
                                                                                                                                                                                                                  tataGCCGCCCTGGTGCACCAGCACTTCCTGATTTTTGACCCACACTTTGCCGTAATGAGTGACCGCATCAAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCttt                 <  1:739294/228‑1 (MQ=255)
                                                                                                                                                                                                                        cgccCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGACCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGGGCTTTCccccca          >  1:426325/1‑229 (MQ=255)
                                                                                                                                                                                                                          cccTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGACACGCAGCACGATACGCTGGCCTGCCCAACCTTTCCGGATAAAGACTTCGCGCTGATACCAGACGCTCTCCGCATAATTCACAAAT‑TCTGCATCGTCGAACTGCCCGTTAAACCTGCCTGGCCCACCAATTGCCCTGCTTTCTTGTAACCCGCCTTCcccccacc        >  2:91614/1‑227 (MQ=255)
                                                                                                                                                                                                                               aggCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGGATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATCATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAACCTGCCTTGCCCAGAAATTGCCCGGGTTTCGTGTAAAGCCCTTTCCCACAAACGCTgc   >  2:721473/3‑228 (MQ=255)
                                                                                                                                                                                                                                  cTCCATCCCTTCCTGATTATTTACCCACACTTTTCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCCGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCTTTCCCACCAACGCTGAt  <  1:73354/227‑1 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                  
TCGGCGTGGTGTAGAGCATTACGCTGCGATGGATCCCGGCATAGTTAAAGAAATCATGGAAGTAAGACTGCTTTTTCTTGCCGTTTT‑CGTCGGTAATCACCATTCCCGGCGGGATAGTCTGCCAGTTCAGTTCGTTGTTCACAC‑AAACGGTGATACG‑TACACTTTTCCCGGCAATAACATACGGCGTGACATCGGCTTCAAATGGCGTATAGCCGCCCTGATGCTCCATCACTTCCTGATTATTGACCCACACTTTGCCGTAATGAGTGACCGCATCGAAACGCAGCACGATACGCTGGCCTGCCCAACCTTTCGGTATAAAGACTTCGCGCTGATACCAGACGTTGCCCGCATAATT‑ACGAATATCTGCATCGGCGAACTGATCGTTAAAACTGCCTGGCACAGCAATTGCCCGGCTTTCTTGTAACGCGCTTTCCCACCAACGCTGAT  >  NC_000913/1695531‑1695980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑