Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,734,567 G→A 15.1% D45N (GAT→AAT)  mepH → murein DD‑endopeptidase, space‑maker hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,734,5670GA15.1% 138.8 / 2.3 55D45N (GAT→AAT) mepHmurein DD‑endopeptidase, space‑maker hydrolase
Reads supporting (aligned to +/- strand):  ref base G (41/5);  new base A (2/7);  total (43/12)
Fisher's exact test for biased strand distribution p-value = 1.16e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAATCGCATTGTGTGAAA  >  NC_000913/1734352‑1734794
                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   
tctcTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGAACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCCTATCACCAAAAACCCAAATaaaaaacaaa                                                                                                                                                                                                                         >  2:94924/1‑223 (MQ=255)
            tGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCCCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAGGCAAGCCTGGTCGAGTTCTGCTACCACTCATATCTCCAAAAAAGCTGATAAAAAGAAAAGCCCGGCAacac                                                                                                                                                                                                             >  1:401253/1‑226 (MQ=255)
            tGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAAGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGCTACACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTAACACCCTTCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGCGAGTTTTGCTACCCAACAAATAACCAAAACACAAAGTAAAAACAAAAGAACgcccaaccc                                                                                                                                                                                                            >  2:443367/1‑221 (MQ=255)
                 tAGAACCATCAAGTGTTGTAAATCAGCCGCAGGCATGATAGACCTGCCTTTACAGGTGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCaaaa                                                                                                                                                                                                       <  1:724277/229‑1 (MQ=255)
                   gAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGCTAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGCGAGTTCTGCTACCAATCATATCACCAAAAAAGCAGAAAAAAAGAAAAGCACGGCAACCACCaaaaaa                                                                                                                                                                                                     >  2:727533/1‑228 (MQ=255)
                              ttttGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTCCCACTCATATCACCAAAAAAGCAGATCAAAAGAAAAGACCGCCAACAAccaaaaaaaccaaaaaaaa                                                                                                                                                                                          >  2:246100/1‑228 (MQ=255)
                                  gTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGc                                                                                                                                                                                       >  1:449095/1‑228 (MQ=255)
                                           aCGCAGGCATGATAGACCTGCCTTTACAGAGTGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCAATGAAACCACCAAAAAGACCCAAAAAACACCGaaaaaaaac                                                                                                                                                                             >  2:574707/1‑226 (MQ=255)
                                            cGCAGGCATAATAGCCCTGCCTTTAAAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTCCGATCCCGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTAAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAgccg                                                                                                                                                                            <  1:445403/229‑1 (MQ=255)
                                             cgcgtcataatgcgccTGCCGTTACAGGGGGCCGCGCAGTTGCTGGGATAAACCGTTTTTCGATCGCTCTCGGGGCTTTGCTTTTTACCCCCCTGCCGTTACCGCTTAGGCCCCATGCTTAAAAGAAAGCAAGGGAGATTTCTGCTACAACTAATATCACAAAAAAAGCAGATAAAAAGAAAAGCCAGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAgccgc                                                                                                                                                                           <  2:397996/215‑1 (MQ=255)
                                                           ccTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCCCCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAgaagga                                                                                                                                                             >  1:548042/1‑224 (MQ=255)
                                                           ccTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTGACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAACGCAAGCCAGGGAGAGTTCTGCTACCAATCATTTCACCAAAAAACCAGATAAAAAGAAAAGCAAGGCAACCACCAACAAAACCCAGAAAACAGCGAAAAAAACCCGCAGTcaaacgacag                                                                                                                                                             >  1:753413/1‑223 (MQ=255)
                                                              gCCTTTCCAGAGGGCCCTTCAGTGGCGGGGATAAACCGTATTCCGATCCCGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTCCCACTCATATCACCAAAAAAGCAAATAAAAAGAAAAGCACGGCACCCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCa                                                                                                                                                          <  1:94924/229‑1 (MQ=255)
                                                               ccTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACACCGAAAAAAGCCGCCAGTAAAAGAAAGACCa                                                                                                                                                          >  1:740090/1‑228 (MQ=255)
                                                                         gggACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCACAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCCGCTAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAAAAGCGAAAAAAACCGCCAGTAAAAGCAACACCAAAAACcaaaac                                                                                                                                               >  2:733914/1‑227 (MQ=255)
                                                                         gggACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATCTCCCCAACAAAGCAGATAAAAAGAAAAACCCGGCCACCACCAAACAGACCCAGAAAAAAGCCAAAAAAGCCGCCAGCAAAAGAACGACCAaaaacaaaaca                                                                                                                                               >  2:165457/1‑228 (MQ=255)
                                                                         gggACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATTACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCACGTAAAAGTACGACCAAAAGCAAAAca                                                                                                                                               >  1:240890/1‑228 (MQ=255)
                                                                         gggACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGACAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAACAAGACCCAGAAAACAGCACACAAAGCCGCCAGGAAAAGATAGACCAAAAGAAAAAca                                                                                                                                               >  1:264894/1‑228 (MQ=255)
                                                                               cTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGcatct                                                                                                                                         >  1:24367/1‑229 (MQ=255)
                                                                               cTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATAACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAAACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCcctcct                                                                                                                                         >  2:186999/1‑229 (MQ=255)
                                                                                    tGGCGCGGATAAACCGTATTTCGATCCCGCTCTGTGCTTTACTTTTTACCCCCCTGCCTTTAACGCCTATGGCCCATGCTTAAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACAAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGAcc                                                                                                                                                           <  1:130752/206‑1 (MQ=255)
                                                                                    tGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCCCCAAAAAGACCCAGAAAAAAGCGAAAAAAGCCGCCACAAAAACAACGACCCAAAACAAAAcccataacctaa                                                                                                                                     >  2:130752/1‑218 (MQ=255)
                                                                                                aCCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAACCGCCCGTAAAAGTACGACCCAAAGCAAAAACGCTTCTTCCGTTAAAAAATCTTc                                                                                                                        >  1:452838/1‑229 (MQ=255)
                                                                                                       ttcaaccACCCTCTGTGTTTTCTTTTTCCCCCCCCTGCTTTTAACCCGTATGGCCATGGCTAAAAAGAAGACAAGGGAGAGTTCGGCCACCCACCAAATCACCAAAAAAGCAAAAAAAAAGAAAAGCACGACACCCACCAAAAAGACCCAAAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTAcc                                                                                                                 <  2:449095/223‑1 (MQ=255)
                                                                                                                    tgtgCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTCCGACCCAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATAACCCCTTCCAAAAAcc                                                                                                    >  2:675451/1‑228 (MQ=255)
                                                                                                                      tgCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCCAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAATAAAAGCACGACCAAAAGCAAAACCGATTCTTACGTTAACAAATCTTCCATTACCGATTCTAAAAACGcc                                                                                                  >  1:616605/1‑229 (MQ=255)
                                                                                                                      tgCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAACACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGATTCTTCAGTTAAAAAATCTACCATTACCGCTTCTaaaaaaccc                                                                                                  >  2:507051/1‑225 (MQ=255)
                                                                                                                      tgCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGAACGACCAAAAGCAAAACCGATTCTTACGTTAAAAAATCTTCCATTACCCCTTCTAAAAACAcc                                                                                                  >  2:752953/1‑229 (MQ=255)
                                                                                                                      tgCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGAACGACCAAAACAAAAACCGCGTCTTCCGTGAAAAAATCTTCCATAAccccaccccaaaacacc                                                                                                  >  2:379036/1‑214 (MQ=255)
                                                                                                                      tgCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAAAAGCGAAAAAAGCCCCCCGTAAAAGTACGACCAAAAGCAAAACCGATTCTTCCGTTAAAAAATATTCCATTACCGCTTCTAAAAACGc                                                                                                   >  1:710783/1‑228 (MQ=255)
                                                                                                                           tACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGCTAAAAAGAAAAGAACGGCAACCACCACAAAAACCCAGCAAACAACCAAAAAACACGCCACTAACAGTCCGACCAAAAGCAAAACCGCCTCCTCCGTTAAAAAAAATACCATTACCGCTACAAAAAAACCCaaacc                                                                                             >  2:248110/1‑227 (MQ=255)
                                                                                                                           tACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACCCCAAAAc                                                                                             >  1:80028/1‑229 (MQ=255)
                                                                                                                            tcTTTTCCCCCCCCTGCCTTAAACCCCTAGGCCCAACACTAAAAACAAAGACAGGAAAATTACCGCAACCACAAACAACACAAAAAAAGAAAAAAAAAAAAAAAGCGCGGAAACCACAAAAAAGACCAAAAAAACCGCAAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACt                                                                                            <  2:240288/228‑1 (MQ=255)
                                                                                                                                tttACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGCAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTACAAAATCTTCCATTACCGCTTCTAAAAACGCCACAACt                                                                                            >  1:240288/1‑225 (MQ=255)
                                                                                                                                tttACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACAAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCATCTAAAAACGCCAAAACTCgc                                                                                         >  1:512599/1‑228 (MQ=255)
                                                                                                                                            ccTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCg                                                                            >  1:84718/1‑229 (MQ=255)
                                                                                                                                            ccTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCg                                                                            >  1:499735/1‑229 (MQ=255)
                                                                                                                                            ccTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACAAACAAAAGCAAAACCGCTTCTTCCGTTAAAAACACTTCCATTACCGCTTCTACAAACGCCAAAACCCCCACCaaaaccaccg                                                                            >  2:655692/1‑222 (MQ=255)
                                                                                                                                            ccTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAATACGACCAAAAGCAAAAACGCTTCTTCCGTTAAAAAATCTTCCATTACCGCCTCTAAAAAAGCCAAAACTCCCAGCAAACACGCCg                                                                            >  2:516891/1‑229 (MQ=255)
                                                                                                                                                      cTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTACAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAAc                                                                  >  1:43509/1‑229 (MQ=255)
                                                                                                                                                      cTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGAAAAACCGCATCTTCCGTTAAAAAATCTTCAATTACCGCCTCAAAAAACACCAAAACTCCACAAAAAAACACCGTCAAAAAAAc                                                                  >  2:278754/1‑229 (MQ=255)
                                                                                                                                                       tATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAAc                                                                  >  1:427418/1‑228 (MQ=255)
                                                                                                                                                                tGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACAAGCCGTCAATAAAACGGCCCcagcc                                                        >  1:342711/1‑229 (MQ=255)
                                                                                                                                                                tGCTTCAAAGCAAGCCAGGGAGAGTTATGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTACGTTAAAAAATATTCCATTAACGCTTCTAAAAACGCCAAAACTCGCAGCAAAAACGCCGGCAATAAACAGGCCTaagcc                                                        >  1:396357/1‑229 (MQ=255)
                                                                                                                                                                         gcaagcCGGGAAAATTTCTGCTCCCACCAAAACCACAAAAAAACAAAAAAAAAAGAAAGGCACGCAAACAACAAAAAAAACCAAGAAAACAGCAAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTAcc                                               <  1:246100/229‑1 (MQ=255)
                                                                                                                                                                                       gTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCCTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACCCGCCGTCAATAAAACGGCCCCAGCCCGCTTTACCGAAAAggggacaaa                                 >  2:552425/1‑221 (MQ=255)
                                                                                                                                                                                             ccaCCACGCAAAACACAAAAAAAGCAGAAAAAAAGAAAACCACGGCAACCACCAAAAAGACCAAAAAAACAGCAAAAAAAGCCGCCAGAAAAAGTACGACCAAAAGAAAACCCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTa                            <  1:516891/226‑1 (MQ=255)
                                                                                                                                                                                              aaccaccaataccccaaaaaaaGCAAAAAAAAAAAAAAGCCCGGCAACCACAAAAAAGCCCCAAAAAACAGCAAAAAAGGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTaa                           <  1:727534/212‑1 (MQ=255)
                                                                                                                                                                                                  aCTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACCCCGTCAATAAAACGGCCCCAGCCAGCTTTACCCAAAAACGTACCCAACTCAAgggaa                      >  2:472665/1‑227 (MQ=255)
                                                                                                                                                                                                   ccaaaacccaaaaaaaaGAAAAAAAAAAAAAAAGCACGCCAACAACAAAAAAGCCCAAAAAACCGGAAAAAAAACCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCt                      <  1:675452/217‑1 (MQ=255)
                                                                                                                                                                                                     cATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGGGTACCAAACGAAAGGGCtata                   >  1:477715/1‑229 (MQ=255)
                                                                                                                                                                                                            aaaaaaaaGAAAAAAAAAAAAAACCCACAGAACACACCAAAAAGCCCAAAAAAAGAGCGAAAAGATCCGCCAGTAAAAGTACGACCAAAAGAAAACCCGCTTCTCCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAAGCGCa            <  2:628486/227‑1 (MQ=255)
                                                                                                                                                                                                             cAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAACCGTAAGGGCTATAAATCGCa            >  1:139257/1‑228 (MQ=255)
                                                                                                                                                                                                                aaaaGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTCAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGACAACACTCCGTCAATAAAACAGCCTCAGCCAGCTTTACCGAAAAGTGACCCAAACGTAAGGGCTATAAATACCATtgt        >  1:265394/1‑229 (MQ=255)
                                                                                                                                                                                                                aaaaGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAAAACACCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAATCGCATtg         >  1:628487/1‑228 (MQ=255)
                                                                                                                                                                                                                       gATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAATCGCATTTGGTGaaa  >  1:136728/1‑228 (MQ=255)
                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   
TCTCTTCTTCTATGCATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAAAAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAATCGCATTGTGTGAAA  >  NC_000913/1734352‑1734794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: