Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,079,975 | A→G | 18.6% | L206P (CTC→CCC) | yeeA ← | putative transporter, FUSC family inner membrane protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,079,975 | 0 | A | G | 18.6% | 156.4 / 3.4 | 79 | L206P (CTC→CCC) | yeeA | putative transporter, FUSC family inner membrane protein |
Reads supporting (aligned to +/- strand): ref base A (29/31); new base G (13/4); total (43/36) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.41e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTCAGCAGTATTTGCTGCATCATGTGCTGGGTATCAC‑GAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGA > NC_000913/2079750‑2080195 | cTCAGCAGTATTTGATGCATCATTGGCTGGGTTTCAC‑GAAGGTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGt < 1:281824/228‑1 (MQ=255) agcagTATTTGCTGCATCATGTGCTGGGTATCAC‑GAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGGCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGGGCA‑ATCAGT‑CCACGCATTTTCCTGGGATCGGTCAGGGGgtttt > 1:210633/1‑229 (MQ=255) tgctgaATCATGTGCTGGGTATCAC‑GAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATGCCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATgg < 1:89163/228‑1 (MQ=255) ctcgcatGTTCTGGGTCTCCCGGAGGTTTTTGC‑CGTTTCATGACCCGAAATGGCTGGGGCGCGTGGCCCAGTATTCGTTGATTTGCACATCCAGCAGACAAACCAGATTTCGGTTAATTGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTc < 2:756525/225‑1 (MQ=255) tgtgCTGGGTATCAC‑GAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGTCCCACCCTTTTTC‑CGCGCTCCGTCCGGGGTTTTTTTGGATGGGTTTTCacccg > 2:426028/1‑225 (MQ=255) gCTGAGTACCAC‑GAAGGTTTTCCGCGTTGAATAACTCGAAATGTCTGGCGCGCGGAGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCGTCATATATCGCATTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑AACAAT‑CCGAGCGTTTTCAAGGCATCGGTCAGGGGTTTTTGGAGATGGATTTCCAG‑ACGttg > 2:172232/1‑227 (MQ=255) ggTATCAC‑GAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGt < 1:535442/229‑1 (MQ=255) ggTATCAC‑GAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTCCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGGTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGt > 1:406897/1‑229 (MQ=255) ccc‑GAATTTTTTGCGGGTTTAATAACACGAAAGGGGCGGGGGGGGGGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTCCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAg < 2:406897/227‑1 (MQ=255) cAC‑GACGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGGA‑ATCAGT‑CCACGCCTTTTCACGGCATTGGTCAGGGGTTTTTTTAGATGGCTTTCCCG‑ACGTGGGCGTTCggg > 1:58669/1‑227 (MQ=255) cAC‑GAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGGTTTTGTAGATGGCTTTCCAT‑ACGTGGGCGTTCggg > 1:61289/1‑227 (MQ=255) cgtaGTTTTTGCGGGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCAGTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATTCCTTCATATATCGATTTTGGAATGCGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGt < 1:216459/225‑1 (MQ=255) cgTTTTTGCGCGTTCAATAACAAGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTATGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGGGCACATC‑GT‑CCCACCATTTTTCAGCCATGGGTCAGGGGGTTTTCTGAATGGCTTTCCAA‑ACCTGTGCgatgacggaagt > 2:486589/2‑218 (MQ=255) aGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCCGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGCTTTTGGAATACGGGTTTCTTTGCTGCCGGGCGCA‑ACCACT‑CCACGCCTTTTCAGGGCATCGTTAAGGAGGTTTTTGAGATGGGTTTCCAG‑GCGTTGGGGTTCGCGTAAGt > 2:232999/1‑229 (MQ=255) tttttGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGC‑CCACGCATTTTTACGGTACCGGTCAGGAGGTTTTTTAGATGGCCTTTCAG‑ACGAGGGCGTTCGAGTAAGtt > 1:68590/1‑228 (MQ=255) tttttGCGCGTTCAATAACACGAAATGCCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCGGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCCCGCATTTTCACGTCATCGGGCAGGAGTTCTTGTAGATGGCTTTCCAG‑ACTTGGGCGTTCGataaaagtc > 1:230421/1‑221 (MQ=255) tGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGCTAATGGTCTGGTTACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCCGA‑ATCAGT‑TCCCACCATTTCACGGCATCGGGTCGGGGTTTTTTTTGATGGCTTTCCCA‑CCTTGGGGGGTCGAGGAAGTTcaggg > 2:427513/1‑225 (MQ=255) ttCAATAACACGAAATGGCTGTGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGGCTGGATACCTTCATATATCGATTTTGGAATCCGGGTTTCTTTGCTGGCGGGCGCA‑ATTAGT‑CCCCGCATTTTCCCGGCACCGGGCAGGAGTTTTTGTAGATGGCTTTCCCA‑ATGTGGGCGGTCGAGTAAGttgtgtggggaag > 1:334087/1‑218 (MQ=255) cacGAAATGGCTGGGGCGCGTGGCCCAGTATGGATTGATTTGCAACTCCAGCATACAAATCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTg < 2:562000/228‑1 (MQ=255) cGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGGTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCACCGGTAAGGGGGTTTTGTGGGAGGCGTTCCAG‑ACGCGGGCGTTCGGGGAAGTTCGGGGGGAATGCAGATTGATa > 1:136308/1‑229 (MQ=255) gAAATGGCTGGGGTGCGTTGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCGGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATa < 1:158923/228‑1 (MQ=255) aaTGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGGGTTTTTGTGAGTGGGTTTCCGG‑GCGTGGTGGTTTGGGTAAGGTCGGTGTGAATGcgagttttatga > 1:712705/1‑217 (MQ=255) aaTGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCCG‑ACGTGGGCGTTCGGGTAAGTTCGGTGAGAATGCAGATTGATAGa > 1:22005/1‑228 (MQ=255) aggggtGGGGCGCTGTGCCCAGTATTGCTTGTATTGCAAACCCAGGATACAAACCAGATTGCGGTTAATGGGCTGGGTTCCTTCATATATCGATTTTGGAATGCGGGTTTCTTTGCTGGCTGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCAGCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAcc < 2:334087/224‑1 (MQ=255) tccacgtattgatttgtttGCAAACACAGCATACAAACCAGTTTTCGTTTATTGGTTTGGATACCTTCAAATATCTGTTTTGGAATACTGGTTTCTTTGCTGGCGGGCGTA‑ATCAGT‑CCACGCATTTTCACGGCATTGGTCAGGAGTTTTTGTAGATGTCTTTCCAG‑ACGTGGGCGTTCGAGGAAGGTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCa < 2:126923/213‑1 (MQ=255) cccAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCC‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGGGGTTTTGTAGATGGCTTTCCCG‑CCGTGGGCGTTCGGGTAAGTTCGGTGGGAATGCCGATTGAAAGACCCGGTTATAATCGGTCAg > 2:238015/1‑229 (MQ=255) gCATTGATTTGCAACTTCAGCATACAAACCAGAGTGCGGTTAATGGTCTGGATTCCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATGCTCGGTCAGACTTTTCg < 1:232999/229‑1 (MQ=255) tGATTTTCAATTTCAGCATGCAAACCAGATGTCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTGCTTTGCCGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAg < 1:470828/229‑1 (MQ=255) aTTTGGAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCGGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGtt < 1:585715/229‑1 (MQ=255) aTTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTTATAGACCCGATTTTATTCGGTCCGACTTTTTGCCACtt > 1:515196/1‑229 (MQ=255) aTTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCAAGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGGTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGGGTGAATGCTGATTTATAGAACCGATTTTACTCGGTCAGACTTTTCGCCAGtt > 1:600948/1‑229 (MQ=255) tttGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCCTTTTAACGGCATCGGTCAGGAGGTTTTGTAGCTGGCTTTCCAG‑ACGTGGGGGTTCGAGTAAGTTCGGTGAGAATGCAGATTGGTAGACCCGCTTTTACTCGGGCAGAACTTTTGCCAATTg > 2:76878/1‑229 (MQ=255) ccAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCCATTATACTCGGTCAGACTTTTCGCCCGTGGaaatggcc > 1:736746/1‑222 (MQ=255) aCCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAAc > 1:449716/1‑228 (MQ=255) aCCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGGCAGGAGGTTTTGTAGATGGCTTTCCCG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGGTGAATGGGCCAGTgggttaaa > 1:442714/1‑222 (MQ=255) ccaagttgtgtttAATGGGCTGGATAACTTCCAATTTGGATTTTGGAATTGGGTGTTCTTTGCTGGCGGGTGCA‑ATCCGT‑CCACCGATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACGCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGc < 2:498599/218‑1 (MQ=255) gATTGCGGTTAATGGTCTGGATCCCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGc < 1:537053/226‑1 (MQ=255) aTTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCCTCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGGGGGAATGCAGATTGATAGACCCGATTATACTCGGGCAAGCTTTTCGCACGTTGAAATCGCCCGTGGGTGAAACCCC‑gg > 2:57239/1‑228 (MQ=255) aTTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTAACGGCATCGGTCAGGGGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGGGTAAATTCGGTGGGAATGAAGGTTGAAAGACCCGGTTATACTCGGTCAGACTTTTCGCCACGTGAATTCGCCGGGGGAGGaaacgccgt > 2:219844/1‑222 (MQ=255) aTTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTGTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTTACGGCCTCGTTCAGGCGTTTTTGTAGATGACTTTCCAGAACGT‑GGCTGTCGAGTAAGTGCGGTGTGATGGCGTATTGATAAACCCGATTAGACTCGGTCAGACTTTTTCACATGCGCATGAGCCAGTGGATGAACGCCC‑G‑t > 2:534124/1‑229 (MQ=255) aTGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAc > 1:286811/1‑161 (MQ=255) aTGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAc < 2:286811/161‑1 (MQ=255) tggtattgaatgcTTCATTTTTCGATTTTGGCATACGGGTTTCTTTGCTGGCGGGCGCA‑GTCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGa < 1:143917/216‑1 (MQ=255) tGGGCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTGTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGa < 1:732728/228‑1 (MQ=255) tggactccTTCATATATCGTTTTTGTAATTGGGGTTTCTTTGCTGGCGTGCGCA‑ATCATT‑CCACGCATTTGCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCg < 2:210633/222‑1 (MQ=255) tGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGAc < 1:507731/155‑1 (MQ=255) ttCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGGGGTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGCGTAAATTCGGTGAGAATGCAGATTGATAGACCCGATTATACACGGTCAGCCTTTTTGCCAGTTGAATGCGACAGTGGATGAAAGGCC‑GCTTGGGGCGA‑AAACCGGTaacaaaa > 2:499670/1‑224 (MQ=255) ttCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGCTCGGTGAGAATGCAGATTGCTAGACCCGATTATAACCGGTCAGACTTTTTGCCAGTTGAATGCGCCCGCGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAaaccac > 1:242104/1‑229 (MQ=255) cattttttgATTTTGGAATACGGGTTCATTTGCTGGCGGGTACA‑CAGAGT‑CCGCGCGTTTGCAGGGCATCGGTCAGGGGCGTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGGAAGTTCGTTGAGAAGGCAGAGTGATAGACCCGATGATACGCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCTGTAAacaacag < 2:89395/221‑2 (MQ=255) cATATTTCGTTTTTGGAATCGGGGTTTCTTGTCTGGCGGGCGCA‑ATCAGT‑CCACGAATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACAt < 2:46052/229‑1 (MQ=255) aatatttCGATTTTGGAATACGGGTTTCTTTGGTGGCGGGTGGA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGATTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACAt < 2:334447/228‑1 (MQ=255) tatatCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCCTTTTCACGGCATCGGTCAGGGGGTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTTGGAATGTGGATTGATAAACCCGATTATACTCGGGCCGACTTTTCGCCAGTTGAATGGGCCAGTGGGTGAACGCCCGG‑TTGGGCCCAGA‑AACGGTAAAAAACAttc > 2:646382/1‑228 (MQ=255) tatatCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGTTTGATAGAACCGGTTATTATCGGTCAGCCTTTTCGCCAGTTGAATGCGCCAGGGGATGAAACCCC‑G‑TTGTGGCCACATACCGGTAAAAAAAAtt > 2:296364/1‑228 (MQ=255) tcgcGATTTTGGGAACCGGGTTTCCTTGTGGGCGGGCGCA‑ATCAGT‑CCACGAATTTGCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTAGAGTAAGTTCGGTGAGAATGTAGATAGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGc < 2:727294/225‑1 (MQ=255) cGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGGGTTTTTGTAGATGGCTTTCCCG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGGTTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCCGTTGAATGCGCCCATGGATTAAACCCC‑G‑TTGTGGCCAGTAACCGGTGAAAAACATTGcccca > 2:102026/1‑226 (MQ=255) cGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGGGAGAATTCAGATTGATAGACCCGATTAAACTCGGTCAGACTTTTCGCCCGTTTAATGCGCCAGGGGGTGAACGCCC‑C‑TTGTGGCCAGATACCGGTAaacacacttgcccgt > 1:217966/1‑218 (MQ=255) ttttGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATTAGT‑CCACGCATTTTTACGGCCTCGGTCAGGAGTTTTTTTAGATGGCTTTCCCG‑ACGTGGGCGTTCGGGTAAGTTGGGGGAGAATGCGGGTTTATAAAACACATCATTCTCGGCAAGACTTTTCGCCCGCTGGATGGTCCCACGGATCAAAGCCC‑G‑TTGTGGCCAGATACCGGGAAAACACAATGccctctaa > 2:113642/1‑223 (MQ=255) tGGTATTCGGGTTTCTTTGGTGGCGGGCGCA‑AACAGT‑CCACTCATTTTCACGGCATCGGTCAGGGTGTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTGCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAagag < 2:22005/229‑1 (MQ=255) ggatgcGGGTTTTGTGGCTGGCGGGCTCA‑ATCAGT‑CCACGCAGTTTGACGGCAGCGGTCAGGAGTTTTTGGAGATGGATTTCCAG‑ACGGGGGCGTTCGAGTAAGGTCGGGTAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑GTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGcc < 2:429088/224‑1 (MQ=255) gtgggctCA‑ATCAGT‑CCACGCATTTTAACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACGCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCtt < 1:672738/226‑1 (MQ=255) ggggggtgaatcgtcacgctccttttCGCGGATTCGGTCAGGAGGTTTTGTAGATGGGTTTCCAA‑ACGTGGTGGTTCGAGTAAGTTCGGTGAGAATGCAGGTTGAGAGACCCGATTATACTCGGTCAGACTTTTGGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCtt < 2:206046/206‑1 (MQ=255) cgcA‑ATCAGG‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCAt < 1:57239/229‑1 (MQ=255) actcgtcccccctttttCCGGGCATCGGTCAGGAATTTTGTTAGATGGCTTTCCAG‑GCGTGGGCGTTCGAATAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACg < 2:242104/216‑1 (MQ=255) cGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGCCTTTTCGCCAGTTGAATGCGCCAGCGGATGAACGCCC‑G‑TTGGGTCCCGGTACCGGTAAACAACATGTCCCGTAAAACGCCGAGGAGCCAATCACCCGTTCACCATAACGCCCTACCaa > 2:237004/1‑229 (MQ=255) cGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCaa > 1:452060/1‑229 (MQ=255) cGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCACCGCTTCGCCATAACGCCGt > 1:697655/1‑224 (MQ=255) cATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAAtt > 1:281294/1‑229 (MQ=255) cATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCCCCGCTTCGCCAAAAAGCCGGATCAAtt > 1:364381/1‑229 (MQ=255) ttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑Ttg < 1:618825/146‑1 (MQ=255) ttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑Ttg > 2:618824/1‑146 (MQ=255) ttCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCGGCTTCCCCATAACGCCGTATCAATTTCAc > 1:296316/1‑229 (MQ=255) tccggggatgtggtaGGATTTTTTGTAGATGGCTTTCCCG‑ACGGGGGCGTTCGATGAAGTTCGGGTGGAATGCAGATTGATAGACCCGATTAGACTCGGTCAGACTGTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCAc < 2:544551/214‑1 (MQ=255) ggCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCCTAACGCCGTACCAATTTCACCttc > 1:574537/1‑226 (MQ=255) cATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCCGATACCGGTAAACAACATTGCCAGTAAAGAGCCCAGGATAAAATCGCCCCTTCCCCATAACGCCGTAACAAATTCACCTGGCgg > 2:663523/1‑229 (MQ=255) aTCGGTGAGGGGTTTTTGTAGATGGCTTTCCGG‑ACGGGGGCGATCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGGCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCggg < 2:736745/229‑1 (MQ=255) aTCGGTCAGGGGTTTTTGTAGATGGCTTTCCAG‑ACGGGGGCATTCGGGTAAGTTCGGTGAGAATGCAGATTGATAGGCCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATCCCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCggg < 2:574536/229‑1 (MQ=255) ggggTCAGGAGTTTTGGTAGATGGCTTTCCAG‑GAGTTGGCGTTCGAGTAAGTTCGGTGAGGATGCAGATTGATAGCCCCGATTATACTCGGTCAGACTTTGCGCCAGGTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCgggg < 1:580125/227‑1 (MQ=255) tggTCAGGTGTTTTGGGAGGTGGGTTTCCAG‑ACGTGGGCGTTCGAGGAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTAAACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGa < 2:281294/228‑1 (MQ=255) cGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTTTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGGGGATCACACCGCCGCTTCGCCCTAACGCCCGATCAAATTACCCTAGCGGGGa > 2:412879/1‑229 (MQ=255) | CTCAGCAGTATTTGCTGCATCATGTGCTGGGTATCAC‑GAAGTTTTTGCGCGTTCAATAACACGAAATGGCTGGGGCGCGTGGCCCAGTATGCATTGATTTGCAACTCCAGCATACAAACCAGATTGCGGTTAATGGTCTGGATACCTTCATATATCGATTTTGGAATACGGGTTTCTTTGCTGGCGGGCGCA‑ATCAGT‑CCACGCATTTTCACGGCATCGGTCAGGAGTTTTTGTAGATGGCTTTCCAG‑ACGTGGGCGTTCGAGTAAGTTCGGTGAGAATGCAGATTGATAGACCCGATTATACTCGGTCAGACTTTTCGCCAGTTGAATGCGCCAGTGGATGAACGCCC‑G‑TTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGA > NC_000913/2079750‑2080195 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |