Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,080,362 A→C 27.6% V77G (GTG→GGG)  yeeA ← putative transporter, FUSC family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,080,3620AC27.6% 56.1 / 2.0 44V77G (GTG→GGG) yeeAputative transporter, FUSC family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (23/7);  new base C (2/12);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.76e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTAT  >  NC_000913/2080138‑2080581
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              
agCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTCACTCCAGTTCCAAAGCGATAAGACCTAAAATCGAACCCAACACCg                                                                                                                                                                                                                            >  2:448398/1‑228 (MQ=255)
     cggATCACATCGCCGCTGCGCCATAACGACGGATCAATTTACCCTGTCGGGGAACCCACAACAATTGCCAGCTTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCTGCCCACCGCAAAGGAACATGGCAGCCGCGCACCAGAGTAACATCAGCGGTAACAAGATTAACTCCAGTTCCAGAACACTAAGAACTAAAAACAAACACAAAACCGTAccac                                                                                                                                                                                                                      >  2:175877/2‑227 (MQ=255)
                gccgcTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAAtcacctca                                                                                                                                                                                                           >  1:41087/1‑222 (MQ=255)
                    cTTCGCCCTAACGCCGTATCAATTTCACCTGTCGGCGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCGTGCCCAACGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGGACCAGGCTAACAACAACGGCAACAAGATTAACTCCGACTGCATAGCGACAAGGCCTAAAACCACACCCAACACAGAACCGCCAAAACGccccacgc                                                                                                                                                                                                       >  2:140728/1‑222 (MQ=255)
                     ttCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCCGACTAACCTCAGCGGAAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGCACCCACAATACCCCCAAAGgc                                                                                                                                                                                                      >  2:442436/1‑229 (MQ=255)
                        cccATAGCGCCGTATCATGTTCCCCTGTCGGGGAACCCACAACAATGCCCAGCGACACCCCAATCAATAACCCTTGATACGTTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTGGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGcgcg                                                                                                                                                                                                   <  1:113329/228‑1 (MQ=255)
                             aaCCCCGTATCAATTCCACCTGTCGGGGAACCCAAAACAATTGCCGGCGTCCCCCCAATCAAAAAACCTTGAGCCGTTTTCTCGCCGAGCGCCAGCAAGCCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACAACAGCGGTAACGAGATTACCTCCAGTTGAAGAGCGATAAGACCTAAAAACGAACCCAACACCGTGCCGCCAATACGCTCAAAGGCGCGAGGGa                                                                                                                                                                                              <  2:254407/229‑1 (MQ=255)
                              tccccttATCAATTTCCCCTGTCGGGGAACCCACACAAATTCCCCGCGTCCCCCCAATAAAAAATCCTTGATGCGGTTTCTGCCCCAGCGCCGGCCAACCGCAAAGAAACATGGCCGCCGCGCACCAGACTAACATCAGCGGAAACGATATAACCGCACGTTGCAGAGCGATAGGCCCAAAAATCGAACCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGAc                                                                                                                                                                                             <  2:41087/223‑1 (MQ=255)
                              acgccggtccattttCACTGTCGCGGGAACCCCGAACCTTTGCCAGCTGCCCCCCATACAAAAAACCTTGATCCGGTTCCTTGCCCAGCCCCAGCAAACCGCAAAGGAACACGGCCGCCGCGCTCCAGACTAACACCAGCGTTAACGACATTAACCCCAGTTGCAGAGCGATAAGCCCTAAAATCGAACCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGAc                                                                                                                                                                                             <  2:391173/217‑1 (MQ=255)
                              acccccttccAATTTCACCTGTCGGGAACCCCACAACTATGGCCAGCGTCCCCACAAGAAAGACCCTCTTGCCCTGTTTCTTCCCCGAGTACAGCACACCGCAAAGGAACATGGCCGCCGCGACCCAAACTACCAACAGCGGTAACGCAATTACCTCCAGTGGCAGAGCGATAAGACCTAAAACCTAACCCTACCCCGTCCCGCCAATACGCTCAAAGGCGCGAGGGAc                                                                                                                                                                                             <  2:515196/220‑1 (MQ=255)
                                    tATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGCACGCTAAAACCTCACATCGAACCCAACCCCCTACCGCCAATCGCCTCAAAGGACCGAGGGCCAACGtc                                                                                                                                                                                       >  2:113329/1‑228 (MQ=255)
                                    tATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCAAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGAGGTAACGAGATTAACTCCAGTTGCAGCGCGATAAGACCTAAAATCGAACCAAACACCGCACCGGCAATAGGCTCCAAGGCGCAAGGGACAAcact                                                                                                                                                                                       >  1:714860/1‑226 (MQ=255)
                                                  gTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAAACCCGGACCGCCAAAACGCTCAAAGGCGCAGGGGACAACGTTACCCCAGacagaaa                                                                                                                                                                         >  2:32503/1‑223 (MQ=255)
                                                         gAACCCACAACAATTCCCAGCGCCCCCCCAAACAAAAACCCTCGATACGGTTTCTCGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACCCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGcc                                                                                                                                                                   <  1:169257/229‑1 (MQ=255)
                                                                caacaaTTGCAAGGCGCACCCCAAGCAATAACCCTGGATACGGTTGCTCGCCCGGCGCAAGCCAACCGCAAAGGAACATGGCCGCCGCGCACAAGACTAACATCAGTGGTAACGAGATTAACTCCGGTTGCAGAGCGATAAGACCTAAAACCGACCCCAACCCCGAACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAAt                                                                                                                                                            <  1:577098/229‑1 (MQ=255)
                                                                      ttGCCAGCGTCACACCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTCACTCCAGTTGCAGAGCGATAAGACCTGAAATCGAACCAAACACCGTACCCCCACTACGCCCAAAGGCCGGCGGGACAACCTTACCCCAGAACGAGATTGGCCCCACAAccacca                                                                                                                                                       >  2:568769/1‑228 (MQ=255)
                                                                                       caccataaaacctttccgcGGTTTCTCCCCCAGCCCCCACAACCCGAAAAGGAACGTCGCCGCCGCGCACCAGACTAACACCAGCGGTAACGAGATTAACTCCAGTGGCAGAGCGAAAAGACCAAAAATCGAACCCAACCCCGTACCGCCAATACGCTTAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGc                                                                                                                                      <  2:323108/211‑1 (MQ=255)
                                                                                       atcaatAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGGGACCAGCGGc                                                                                                                                      >  1:639391/1‑228 (MQ=255)
                                                                                                  cgtgaatcGGTTTCTTGCCCAGCGCCAGCAAACCGAAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACCCCAGTGGCAGAGCGATAAGACCTAAAATCGACCCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTc                                                                                                                           <  1:486589/222‑1 (MQ=255)
                                                                                                     gATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTCCCGCCAATACGCCCAAAGGCGCGAGGGACAACGTTACCCCAGAACAAAATTGGCCCCATAATCACCCCCAAGGTGACCAGCGGCCAGGGGCATTCCgg                                                                                                                        >  2:617983/1‑229 (MQ=255)
                                                                                                         ctgTTTCTTGCCCAGCGCCAGGCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCCAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATa                                                                                                                    <  1:377696/227‑1 (MQ=255)
                                                                                                                  gCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTAGCAGAGCGATAAGACCTAAAAACGAACCAAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGCCCACATAAGCACCACAATGGAGACCAGCGGCCAGGGGCTTTCCGGGAGAGTAAACAgg                                                                                                           >  2:468874/1‑229 (MQ=255)
                                                                                                                      aGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGCTAAGACCTAAAATCGAACCCAACACCGTACCGCCCATAAGCTCAAAGGCGCGAAGGCCAACGTTACCCCAGAACGAGATTGGCCCCATAATCAACACCACGGCGACCAGCGTCCAGGGGGTTTTCGGGATAAGAAACacgagaa                                                                                                       >  2:177038/1‑223 (MQ=255)
                                                                                                                        cgcCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGCGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCCCCATGGTGACCCACGGCCAGGGGCTTTCCGGGAAAGTAACCAGgggggaa                                                                                                     >  2:239743/1‑226 (MQ=255)
                                                                                                                             gccaACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCACAACGGGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGAGCTTTCCGGGGAAGTAACCAGACgggaaataag                                                                                                >  1:721194/1‑221 (MQ=255)
                                                                                                                                  ccGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAACATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGCACAACGTTACCCCAGCACGCGCATGGCCCCATAATCACCACCCTGGTAACCAGCCGGCAGGGGCTTTCCGGGCTAATaaaacagcggagactggagaaag                                                                                           >  2:323347/1‑209 (MQ=255)
                                                                                                                                  ccGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAg                                                                                           >  1:623780/1‑229 (MQ=255)
                                                                                                                                    gaaaaGAAACAGTGCCCGCGCGCACCAAACGAACATGATCTGAAACGAGATTGCACCAGTTTGCAAAGCAATAAGACCTAAAACAAACCCCACCACACGTCCGGCAATACGCTCAAAGGCGCGAAGGGAAACGTTACCCCATAACGAGATTGGCCCCATAATCACCACCAGGGTGACCAGTGGCGAGGTGGTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTg                                                                                         <  2:442714/227‑1 (MQ=255)
                                                                                                                                                    gccgcGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACAAGCGGCCAGGGGCTTTCCCGGATAGTAAACAGGGGGCAAATGAGAAAAGGGAGCAGGAAAGCCaacc                                                                        >  2:543112/1‑226 (MQ=255)
                                                                                                                                                      cgcgccacaagacaaCACCAGCGGTAACGAAATTAACTCCAGTTGCAGGGCGATAAGACCTAAAAGCGCACCCAACACGGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAgcgc                                                                       <  1:428661/215‑1 (MQ=255)
                                                                                                                                                      cgcgCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTCCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAgcgc                                                                       <  1:448398/228‑1 (MQ=255)
                                                                                                                                                      cgcgCAACAGACTAACATCAGCGGAACCGAGATTACCCCCAGTTGCAGAGCGATAAGACCTAAACTCGAACCCACCCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAgcgc                                                                       <  1:239743/228‑1 (MQ=255)
                                                                                                                                                          gacaggaaaaacaacaagggTAACGAAATTAACTCCAGGTGCAGGGCAATAAGACCAAAAATGGAACCCAACCCCGTCCCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGAccc                                                                  <  2:748104/211‑1 (MQ=255)
                                                                                                                                                             caaaCTAACATCAGCGGTAACCATATTAACCACAGTTTCAGAGCGATAAGCCCTAAAATCGAACCCAACCCCTGACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGa                                                                <  1:663524/225‑1 (MQ=255)
                                                                                                                                                             cAGACTAACATCAGCGATACCGAGATTAACCCCATTTGCAGAGCGATAAGACCTAAAACCGACCCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGa                                                                <  1:113642/228‑1 (MQ=255)
                                                                                                                                                                          gCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGCAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAAt                                                  >  1:544824/1‑229 (MQ=255)
                                                                                                                                                                          gCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGAccc                                                                  >  1:748105/1‑213 (MQ=255)
                                                                                                                                                                           cGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGCCCTAAAATCGACCCCAACCCCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACaa                                                   <  1:442436/227‑1 (MQ=255)
                                                                                                                                                                              tAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAGCAGGCGGGTAATTGGAAAAGTGGGCCGGAAGTCCAGCAGGACCCGaaaaaacagcacaaagcgg                                              >  2:262686/1‑211 (MQ=255)
                                                                                                                                                                                              aGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGGTAGTAAACAGGCGGATAATGAGAAAAGTGAGCCAGGAATGCCAGCGCAACCCGGGGACCATGCACAAAGCGGTAATGgggggaaaac                              >  2:520995/1‑223 (MQ=255)
                                                                                                                                                                                                         cgaaaaggctcaaaaTCGAACCCAACACCGTACCGCAAATGCGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCaaa                   <  1:32503/218‑1 (MQ=255)
                                                                                                                                                                                                                 ccTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAAGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGACCAGGTGCTTTCCGGGATAGTAAACCGCCGGTTAATCAGAAAAGTGTGCAGGAATGGCAGCGCGCCCCGAGTACCATGCACAATGCGGCAATGGAGGTATAACACGAGTTCCAAACGGCTTa           >  2:475354/1‑229 (MQ=255)
                                                                                                                                                                                                                    aaaaTCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGAGCGACCCCAGTACCATGCACAATGCGGAAATGGCGGGATACCCgcgtttaccaacggggtcaca        >  2:101263/1‑209 (MQ=255)
                                                                                                                                                                                                                           aaCCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGCAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTCACAAGCACAATGGGGGAATGGCGGGTAAACCGGTATTTAAAAGGGGCTCAAtatttatt  >  2:431378/1‑222 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              
AGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAACCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATCGAACCCAACACCGTACCGCCAATACGCTCAAAGGCGCGAGGGACAACGTTACCCCAGAACGAGATTGGCCCCATAATCACCACCATGGTGACCAGCGGCCAGGTGCTTTCCGGGATAGTAAACAGGCGGATAATGAGAAAAGTGAGCAGGAATGCCAGCGCGACCCGAGTACCATGCACAATGCGGTAATGGCGGTATACCCGGATTTCAAACGGGCTTAATGACTTAT  >  NC_000913/2080138‑2080581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: