Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC100.0% 222.6 / ‑6.7 79I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (33/43);  minor base T (0/2);  total (34/45)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACT  >  NC_000913/2132566‑2133011
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                  
cccGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTg                                                                                                                                                                                                                               >  1:595212/1‑227 (MQ=255)
 ccaaactccATCAGCTAGTATTTTGTCGTTTCGCCCCGAACGCGGGGTCCGCAAAGTGGGCCAGTTTATGCGTTTATTGCGGATCTGGTAGAGGGTAAACATCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTAACCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                                                                                                           <  2:326569/222‑1 (MQ=255)
 ccGAATACCATCAGTCAGTCGTTTGGCTTTCCGCGCCGATGGAGGCGTCAGCAAAGGGCGCCAGGTCATTCGGTTATTGCGGATCTGGTAGGGGGTAAACAGCCGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                                                                                                           <  2:225037/229‑1 (MQ=255)
 ccGAAACCCACCCGCCAGGCGTTTCCCGTTACGCACCGACAGCGGGGTCAGGAAAGGGTGCCAGGTCATACGTTTATTGCGGATCTGGTAGATGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                                                                                                           <  2:340519/229‑1 (MQ=255)
   gAATTCCATCAGCCAGACTTTTGCCGTTTCGTACCGACAGCAGCGTCAGCAATGTGCGCCAGGTCATTCGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                                                                                                         <  1:579628/229‑1 (MQ=255)
   gAATTCCATCAGCCAGACGTGTGCCGTTACTCACCGACAGCAGAGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                                                                                                         <  1:70854/229‑1 (MQ=255)
   gAATACCATCAGCCAGACGTTTGCCGTTACGCCCCGACAGCAGCGTCAGGAAAGTGCGCCAGGTCATACGTTTATTGGGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                                                                                                         <  1:583380/229‑1 (MQ=255)
   aaaTACCATCAGCCGGTCGTTTTCCGTTACGCACCGGCAGCTGGGTCAGCAAAGTGCCCGAGGTCATTCGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                                                                                                         <  1:340790/228‑1 (MQ=255)
        acttacaccAGGCTTTTGCCGGTTCGCACCGACGGCGGCGTGAGCAAAGTGCGTCGTGTCATGCGGTTATTGGGGATCTGGTAGAGGGTACACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTATTTGGGCTTGCGGTAAAAGTGGAATTTCCCGGAGAATTTTTTCGGTTACGAGGTGATCTCCATTTTACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt                                                                                                                                                                                                                    <  2:521498/222‑1 (MQ=255)
         cATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGAAGGCTCTGCGTCGTCtctt                                                                                                                                                                                                                    >  1:521498/1‑228 (MQ=255)
           tCAGCCAGACTTTGGCCGTTGCGCACCGAAAGGAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGGGCTTGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                                                                                                           <  2:700894/219‑1 (MQ=255)
           tCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGCTCTGGTAGAGGGAAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGCGGTGATCTGCATTTCACCGCGATTGATATGCAGGCTCTGCGTc                                                                                                                                                                                                                           >  1:700895/1‑219 (MQ=255)
           tCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTCCATTTCACCGTGATTGATATTCAGGCTCTGAGTCGGCTCTTCTa                                                                                                                                                                                                                 >  2:736092/1‑229 (MQ=255)
                   aCGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGCCGAGGTGATCTGCATTTCACCGTGATTGATTTCCAGGCTTTGCGTCGCCTCTTCTACTTTCCAc                                                                                                                                                                                                         >  1:750853/1‑229 (MQ=255)
                   aCGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGGGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGGGTCGCGTCTTCTACTTTCCa                                                                                                                                                                                                          >  1:729266/1‑228 (MQ=255)
                    cGTTTGCCGTTTCGCACCGACAGCAGCGTCAGCAAAGTGCGACAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg                                                                                                                                                                                                        <  1:437614/229‑1 (MQ=255)
                         gCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGCATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGTGGTGATCTGCATTTCACCGCGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTc                                                                                                                                                                                                    >  1:246311/1‑228 (MQ=255)
                            gtttcgctgcGACAGCGGGGTTAGGAAAGTGGGCCGGGTTATCGGTTTAGTGCGGATTTGGTAGAGGGTAAAAGGCTGATATTTTGTGCTGGCGCGGTCGAATTTGTTTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                                                                                                                <  2:724268/220‑1 (MQ=255)
                             ttACGCACCGACAGCAGTGTCAGCAAAGGGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATAGTTTTTGCTAGCGCGGTCGAATTTGGCTTTGTGCTTGCGGTAAAAGTGGAAGTACCAGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                                                                                                                <  1:212677/228‑1 (MQ=255)
                               aCGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGGAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGTTGACGAGGTGATTTGCATTTCACCGTGATTGATATGCAGGTCTGGCGCGGCCTCTTCTACTTTCCCCCGTTCTCCGTa                                                                                                                                                                                              >  2:501885/1‑228 (MQ=255)
                                        gagcagaGGCAGCAAAGTGCGCCAGGTCTTACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTTATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGATAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccac                                                                                                                                                                                     <  1:594566/227‑1 (MQ=255)
                                                      aagggggcccagTCATACGTTTATTGCGGCTCTGGTAGAGGGTAAAGAGCTGATATTTTTTGCTGGCTCGGTCAAATTTGTCGTTGTGCTTGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                                                                                                                         <  2:729265/215‑1 (MQ=255)
                                                              cAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGAAGGCTTTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCAACCCCCATCCGCAGGAAGagtccgta                                                                                                                                                                >  1:145113/1‑220 (MQ=255)
                                                              cAGGGCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCATGCGGGAAAAGTGGAAGTACCCGGGGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTTGTATGCAGGCTCTGCGTCGCCTCGTCTACTTTCCACGGGTCGCCGTAGTCACCCGGCATACGCCGGAAGAAGtagga                                                                                                                                                               >  2:107389/1‑223 (MQ=255)
                                                                 gtctgtcGTTTATTGCGGATCTGGTAGAGGGTAAACAGGTGATTTTTTTTGCTGGCCCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCt                                                                                                                                                          <  1:371568/223‑1 (MQ=255)
                                                                     tACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGGGATTGATATGCAGGCTCTGCGTCGCCCCTTCTACTTTCCCCGGTTCGCCGTCCCCCCCCACCATCCCCGGGAGATCGTCGTAATcccttg                                                                                                                                                       >  2:145965/1‑224 (MQ=255)
                                                                     tACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGCGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGAAAAATGTCGTAATCCtttg                                                                                                                                                       >  2:148457/1‑228 (MQ=255)
                                                                     tACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTGGTCTTTTTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTCCTTGATAATCAGGCTCTGCGGCGCCTCTTCTACTTTCCACGTTTCGCCGTACACCACAACCACCCCCAGGAAGATGTCGTAATCCTCTGc                                                                                                                                                      >  2:178566/1‑229 (MQ=255)
                                                                      gtttttgtgggggagttGGTAGAGGGTAAACAGTTGATAGTGTTTTCTGGCGCGGGCGAATTTGTCGTTGTGGTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTGTTCGGTGACGAGGTGATCTGCATTTCACCGGGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGcc                                                                                                                                                     <  2:545935/213‑1 (MQ=255)
                                                                           tATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTTGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCGGTACTCCACCACCATCCGCCGGACGCTGTCGTAATCCTGGGCCGCttt                                                                                                                                                >  2:159170/1‑229 (MQ=255)
                                                                            ttgtgggATTTGGGGGAGGGTAAACAGGTGATATTTTTTGCTGGCGGGGGCGAATTTGTCTTTGGGGTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                                                                                                                <  2:246311/223‑1 (MQ=255)
                                                                            aTTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGTAAGATTGCGTAATCCtgtcactcttt                                                                                                                                                >  1:635728/1‑220 (MQ=255)
                                                                            aTTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGGGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGTCGTAATCCTTTGCCGCttt                                                                                                                                                >  1:196241/1‑228 (MQ=255)
                                                                                cGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                                                                                                           >  1:487327/1‑229 (MQ=255)
                                                                                     cTGGTTGGGGGTAAACAGCGGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa                                                                                                                                                                       <  2:498706/197‑1 (MQ=255)
                                                                                     cTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAgc                                                                                                                                                                       >  1:498706/1‑196 (MQ=255)
                                                                                      gttttgagGGTATAGAGGTGATTTTTTTGGGTGTGGCGGGTGAATTTGTTTTTGTGCTTGGGGGAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCTCCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt                                                                                                                                     <  2:294614/224‑1 (MQ=255)
                                                                                              ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaac                                                                                                                             >  1:443908/1‑228 (MQ=255)
                                                                                               gTAAACAGGTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGATTGCGGTAAAAGTGGAAGTTCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                                                                                                             <  1:487519/228‑1 (MQ=255)
                                                                                                taaatgggtGATATCTTGTTCTGGCGGGGTCGAATTTGTCTTTGTGCTTGCGGTTAAAGTGGAAGTACGCGGAGAAGTGTTTCGGTTACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTTGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                                                                                                           <  1:315697/221‑1 (MQ=255)
                                                                                                  aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCCCTTTTCCTTTCCCCGGTTCGCCGTACTCAACCCCCACCCCCAGAGAATTGTCGCAACCCGTTGCCCCTCTCAATCCGGATTCCAACAAaaac                                                                                                                          >  2:70853/1‑228 (MQ=255)
                                                                                                  aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGGAAAAGTGGAAGGACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACTATCCGCAGGAAGATGTCGGAATCCTGTGCCGCTTCCATTTCGGTATCGcacaacacc                                                                                                                          >  2:175918/1‑224 (MQ=255)
                                                                                                       cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAAAACCCttt                                                                                                                     >  1:333090/1‑228 (MQ=255)
                                                                                                        tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATGGAAAAAAACCTCTTTg                                                                                                                    >  2:521149/1‑228 (MQ=255)
                                                                                                        ctgtttttttttGCTGGCGGGGTCGAATTTGTTTTTGTGCTTGGGGTAAAAGTGGAAGTTCCCGGAGAATTTTTTCGGTGACGAGGTTATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt                                                                                                                     <  2:750852/222‑1 (MQ=255)
                                                                                                              ttttttGGTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGGGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGc                                                                                                              <  1:711607/229‑1 (MQ=255)
                                                                                                                    ggtGGCGGGGTGGATTTGGTTTTTGGTGTTTCGGTAAAAGTGGAAGTTCCCGGGGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGcc                                                                                                        <  2:117411/227‑1 (MQ=255)
                                                                                                                    ggtGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGcc                                                                                                        <  1:148457/227‑1 (MQ=255)
                                                                                                                     cTGTCGCGGTGGATTTTGTCGTTGTGGTTGCGTTAATAGTGTAAGTACCCTGAGAAGTTTTTCGGTGACGAGGTGATCTGCCTTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                                                                                                       <  2:145113/229‑1 (MQ=255)
                                                                                                                     cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTGCGCCGTACTCCACCACCATCCGCAGGAGGATGTCGTAATCCTGTGCCGCTTTGAGTTCGGAATCGAACAAACACACTTTGAAACCCCATGcc                                                                                                        >  1:660337/1‑228 (MQ=255)
                                                                                                                      tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                                                                                                          <  2:546014/130‑1 (MQ=255)
                                                                                                                      tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                                                                                                          >  1:546014/1‑130 (MQ=255)
                                                                                                                        gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                                                                                                           >  1:583746/1‑206 (MQ=255)
                                                                                                                        gcgcGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                                                                                                           <  2:583745/206‑1 (MQ=255)
                                                                                                                                   ttttTCTTTGTTGTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTg                                                                                          <  1:521149/228‑1 (MQ=255)
                                                                                                                                    ttGTCGTTGTGCTTGGGGTAAAAGTGGAAGTTCCCGGAGAATTTTTTCGGTGACGATGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTgg                                                                                         <  1:469285/228‑1 (MQ=255)
                                                                                                                                     tttttttGTGTTTGCGGTAAAAGGGGAAGTACCCGGAGAATTTTGTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt                                                                                       <  2:595211/225‑1 (MQ=255)
                                                                                                                                      gttcttGTTCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGGTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAGCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt                                                                                       <  1:736093/224‑1 (MQ=255)
                                                                                                                                      gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt                                                                                       <  1:152226/228‑1 (MQ=255)
                                                                                                                                      gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTGTTTCGGTTACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt                                                                                       <  1:427261/228‑1 (MQ=255)
                                                                                                                                        cTTTGTGCTTGCGGTAAAAGTGGATGTACCCGGAGAATTTTTTCGGTGACGGGGTGATCTTCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGc                                                                                     <  1:510526/228‑1 (MQ=255)
                                                                                                                                                  gctgTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACGCTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTg                                                                          <  1:175918/226‑1 (MQ=255)
                                                                                                                                                       taacgttgAAGTTCCCTGGGAATTTTGTGGGGGACGGTGTGATCTTCTTTTCACCGTGTTTGATCTGGAGGTTTTGCTTCGCCTCTTCGACGTTCCACGGTTCGCAGTACTCCACCACCATCCGGAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCGTTGAAACGCCATGCCCAGGTAAAGATCTGGTTGCCAATGATATTGCGttt                                                                     <  2:635727/222‑1 (MQ=255)
                                                                                                                                                       aaaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCAACATCCGCAGGACGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGACCAAACACTCTTTGAAACGCCATGCCCAGGTAAAAACACGGTTGCCAAAGAAATTGCGttt                                                                     >  2:93004/1‑229 (MQ=255)
                                                                                                                                                             ggAAGTACCCGGAGAATTTTTTCGGTTACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGaa                                                               <  1:501885/229‑1 (MQ=255)
                                                                                                                                                                 gTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCCCCGTTATTGGTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAgg                                                           <  1:504764/229‑1 (MQ=255)
                                                                                                                                                                  tgcccGGAAGATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAggc                                                          <  1:486715/227‑1 (MQ=255)
                                                                                                                                                                    cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAACGACCTGGTTCCCAATGACATTGCGTTTCTAGAACAggcgg                                                        >  1:727312/1‑229 (MQ=255)
                                                                                                                                                                               ttttCGGTTACGAGGTGATCTGCATTTCCCCGTGATTGATAGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACgg                                             <  1:145965/229‑1 (MQ=255)
                                                                                                                                                                                      tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATTCCCAGGTAAAGACCTgg                                                                                         >  1:500272/1‑178 (MQ=255)
                                                                                                                                                                                      tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGAtt                                        <  1:380060/227‑1 (MQ=255)
                                                                                                                                                                                                 tttGCTTTTCCCCGTGTTTGCTCTCCGGGCTCTCCGTGCCCTCTTCTCCCTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCgg                           <  2:333090/227‑1 (MQ=255)
                                                                                                                                                                                                          cACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACAATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGAAAAGACCTGGATGCCAATGTTATTGGGTTTGGAGAACAGACGGCGTGCATACGGCGAGTTGGGAAACAGCTGCAAGCATGc                  >  1:268389/1‑229 (MQ=255)
                                                                                                                                                                                                             cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCTCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACTAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTCCCAATGATATTGCGTTTGTAGTACAGTCGGCGTGACTACGTCGATTTGGGATACAGCGGCCAGCTTGACgg               >  1:225887/1‑229 (MQ=255)
                                                                                                                                                                                                               tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAAAAGGCGGCGTGAAAACGGCGCATTGGGATCCAGCGGCAAGCTTGCCgg               >  1:223483/1‑227 (MQ=255)
                                                                                                                                                                                                               tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGACGACCAGGCGGCGGGAATACGGCCAATTTGGTAACACCGTCAAGCTTGGCGGtt             >  2:233418/1‑229 (MQ=255)
                                                                                                                                                                                                                 attgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGCTGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGCGGGGAATACGGGGGTTTGGGATACAGCGGCAAGATTTCCgggggg           >  2:502524/1‑225 (MQ=255)
                                                                                                                                                                                                                    gatATGCCGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGaa        <  1:233418/229‑1 (MQ=255)
                                                                                                                                                                                                                          gaGGTTCTGCGTCCCGTCTTCTTACTGCCAGGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCGGGGCCGCTTTCAGTTCGGTATCGAACAAACTCTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACt  <  2:220310/228‑1 (MQ=255)
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                  
CCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGAATAGACT  >  NC_000913/2132566‑2133011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: