| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,149,901 | G→C | 46.2% | A362G (GCG→GGG) | yegI ← | protein kinase‑related putative non‑specific DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,149,901 | 0 | G | C | 46.2% | 1.9 / 3.7 | 17 | A362G (GCG→GGG) | yegI | protein kinase‑related putative non‑specific DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (5/4); new base C (2/6); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCAGACTGCCAATAATCCAGATAGCCGCCAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAA‑GCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGC‑AATGCTGGCGGTGCTACTGACGCCATCACCA‑TCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAT‑AAAATAGATAACACCTTGATTATCCA‑GCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTG > NC_000913/2149685‑2150129 | gTCAGACTGCCAATAATCCAGATAGCCGCCAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAA‑GCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGC‑AATGCTGGCGGTGCTACTAACGCCATCACCA‑tt > 1:156401/1‑227 (MQ=255) aacctcacAATAACCCAGATAGCCCCCAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAA‑GCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGC‑AATGCTGGCGGTGCTACTGACGCCATCACCA‑TCGccc < 2:456826/222‑1 (MQ=255) aCTGCCAATAATCCAGATAGCCGCCAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAA‑GCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGC‑AATGCTGGCGGGGCTACTTGCGCCCTCACCA‑TCGCCCa > 1:431160/1‑228 (MQ=255) ccAGATCCCCGCATGCCCGGGAACAAAAATAAAATTACGCTGTTTCGCCTGGAGGGCACAAGGCGGGTGCGATAAGGTTGACAGTGCGGTCCCAA‑GCAGAGTGAGTTATTCGCGCCGTAACGGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGC‑AATGCTGGCGGGGCTACTGACGCCATCACCA‑TCGCCCAGACTTTCGCCAGc < 2:379741/229‑1 (MQ=255) attataACGCGTTTCTGCTAGAGGGCACAAACGCAGACGGGAAAATGGTGAC‑GTGGGATCTCAAGGCAAAATGATTTTTTGGGCCCGAAACAGGCTAAAGGGAAGCGGCCCGCCGGTCGGTTGGAAAGGATCGGGTAATGGCAGTTGC‑AATGCTGGCGGTGCTACGGCCGCCATCCCCA‑TCGCCCAGACTTTCGCAAGCACAAAATTCCCGCCGGTGGTAATGACct < 2:156401/225‑1 (MQ=255) cgcgGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAA‑GCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGC‑AATGCTGGCGGTGCTACTGACGCCAT‑ACCACTGGCCCAGACTTTTGCCCGCGCAAAATTTCCGCCGCGGGGAAGGACCCCTTCTcccg > 1:254262/1‑227 (MQ=255) aaCAGTCCTAAAGGAGGCGGCCTGCCAGTCGGTTGGAAATGATCTGTTAATGGCAGTTGC‑AAGGCTGGGGGTCCTCCCAAGGCCATCACCA‑TCCCCCAGACTTACCCCAGCAAAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAT‑AAAATAGATAACACCTTGATTATCCA‑GCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTg < 1:622703/229‑1 (MQ=255) aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGC‑AATGCTGGCGGAGCTACCGCCCCCATAAACA‑CCCACCAAGCCTTTTCCAGCCCAAAAATCCCCCCCGGGGGAAAGACCTCTCCCCCCTGATTCAC‑AAAAAAAACAACACACTGATTTTCCACGACCAAACCGCGGGCAGCCAGCAAAAGGAG‑GGGGAAAACAtatagtgcgtaaaagaaaaaat > 2:510119/1‑208 (MQ=255) ccTGCCAGACGGTTGGAAATGATCGGGTAATGGCAGTTGA‑AATGCTGGCGGTGCTCCTTACGCCATCACCA‑ACCCCCAGAATTTCGCCCACACAAAATTTCCGCCGGGGGGAATTACCCCTTCTCCGGGGCCAATAAAAAAACAAAACACTTTG‑TTTTCCC‑GGGCCCACCCCAGGCAGGCAGTTAAAATGGCCGGGGAAAAAAGCCTTGCGCAAAAAACAAATTTCttt > 2:185406/1‑229 (MQ=255) gtggggttgAAATGGTCGGGTAATGGCAGTTGC‑AAGGGGGGGGGTGCACGCGGCCCCATCCCCC‑TCGCCCGGACTGTCCCCAGCAAAAAATTCCCGCCGGTGGTAATGACCTCTGCGCCGAGATCAAT‑AAAATAGATAACACCTTGATTAGCCA‑GCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg < 2:77847/219‑1 (MQ=255) gAAATGATCGGGTAATGGCA‑TTGCAAATGCTGGCGGTGTTACTGACCCCATCCCCA‑TCGCCCAGACTTCCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAT‑AAAATAGATAACACCTTGATTATCCA‑GCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTaga < 1:632520/229‑1 (MQ=255) agggttgtgttggggCAGGTGC‑AATGCTGGCGGTGCTACCTACCACATCACCA‑CCCCCCAAACTTTCGCCAACACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAT‑AAAATAGATAACACCTTGATTATCCA‑GCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTaga < 2:431160/213‑1 (MQ=255) aggctCGGGTAAGGGCAATTGC‑AATGCGGGCTGGGCTACTGACCCCATCACCA‑TCGCCCAGATGTTCAACTGCAAAAAATGCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAT‑AAAATAGATAACACCTTGATTATCCA‑GCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTaga < 1:699217/222‑1 (MQ=255) gggTAATGGCAGTTCC‑AATGCGGGGGGTGCAACTGACCCATTCACCA‑CCGCCCAGGTTTTCGCAAGCAAAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAT‑AAAATAGATAACACCTTGATTATCCA‑GCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAg < 1:119939/228‑1 (MQ=255) ctggcctgtGC‑AATGCCGGGCGTGCATCTGCACCCCTCACCC‑TCGCCCAGCCTTCCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAT‑AAAATAGATAACACCTTGATTATCCA‑GCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGc < 1:721944/221‑1 (MQ=255) ggCGGAGCTACTGACCCCCTCACCA‑TCGCGCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGAACTCTTTGCCGAGGTCAAT‑AAAATAGCTAACCCCCTGATGATCCA‑CCGCCCACCACGTGCAGTCATTCAAAACAGCAGGGAAAACATGCATTGCTGAAACGAAAACTTTCTTTAAATGTTGTGGAAGAGAGTCACGTCACGCTCCCCACGCTTTAGcca > 2:177461/1‑228 (MQ=255) gCGGTGCTACCGACGCCATCAACA‑TCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGGGGTGATGACCTCCTCGCCCAGATCAAT‑AAAATAGATAACCCCTTGATTATCCA‑GCCCCCACCACGGGCAAGCAGTCAAATGAGCGGGGGAAACATGCATTGCTGAAACGATTAATTTCTTTAACTGTTTGCGGAAAGAGGCCAGTGCCGCTACCCACGCTTTAGccct > 2:222965/1‑227 (MQ=255) gCCATCACCA‑TCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAT‑AAAATAGATAACACCTTGATTATCCA‑GCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGAAGAGAGTCCAGTGCCGCAACCCACCCTTTAACCGTCGGACGCCCGGTTg > 2:289036/1‑229 (MQ=255) | GTCAGACTGCCAATAATCCAGATAGCCGCCAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGCAAAGCAGCAGCGATAATGCTGACAGTGCGATCTCAA‑GCAGAATGATGTATTCGCGCCGTAACAGGCCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGC‑AATGCTGGCGGTGCTACTGACGCCATCACCA‑TCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAT‑AAAATAGATAACACCTTGATTATCCA‑GCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTG > NC_000913/2149685‑2150129 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |