Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,235,396 T→G 21.7% V45G (GTG→GGG)  preA → dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,235,3960TG21.7% 78.8 / 3.4 57V45G (GTG→GGG) preAdihydropyrimidine dehydrogenase, NADH‑dependent, subunit C
Reads supporting (aligned to +/- strand):  ref base T (19/21);  new base G (13/2);  total (34/23)
Fisher's exact test for biased strand distribution p-value = 1.30e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGC  >  NC_000913/2235180‑2235623
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         
ggTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGGAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCCACTGCTATTGGATGGGTGCCAAAGCCTACGACACCGGTTGGGGCGGGTGGGAGTTTTAAACGAt                                                                                                                                                                                                                               >  1:127864/1‑229 (MQ=255)
ggTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTTTGCCTCTCTTCTTCGCCGGTAGGCCACTGCTATGAGTTGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGGGTGTGGTTTTAAACGag                                                                                                                                                                                                                               >  1:489006/1‑228 (MQ=255)
ggTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGGAACTGCTATGAGATGTGTGCCACAGCCTACGAACCCGGTTTGGGCGGGGGGGGGTTTAAAAAGaa                                                                                                                                                                                                                               >  2:717636/1‑228 (MQ=255)
ggTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACGATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAGGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCATTTAGATGTTTGCCAAACCCTACGACCCGGGTTGGGGGGGGTGGGGTGTTAAAAAcaa                                                                                                                                                                                                                                >  2:104239/1‑227 (MQ=255)
   gACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTCTGAGATGTGTGCCAAAGCCTACGAACCAGGTTGGGGCGGGGTGGGGGTTAAAACGATCgg                                                                                                                                                                                                                            >  2:25060/1‑229 (MQ=255)
     aaaaaCCGTGGTCGAGGCCGTGAAACCCGGGAAAGAAGCCGCCGAGGCGATTCCTCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCt                                                                                                                                                                                                                          <  1:589793/228‑1 (MQ=255)
             tGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCCATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGGGGGGTTTAAAACGAACGGCTTTTTTAtt                                                                                                                                                                                                                  >  2:444872/1‑228 (MQ=255)
              ggTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTTAAACGATCGGCTTTTTTATc                                                                                                                                                                                                                  >  1:706372/1‑228 (MQ=255)
                    tGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGCGGGTTTGGTGTTTAAAACGATCGGCTTTTTTTTCGCCAAc                                                                                                                                                                                                            >  1:561385/1‑228 (MQ=255)
                         tGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTATAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCTCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGCCACAGGGTGGGGCGGGGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGCAGtc                                                                                                                                                                                                     >  1:198931/1‑229 (MQ=255)
                         tGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAACGATCTTTCGCTTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTATTCGCCGGTCGGCAACTGCTATGAGATGGTTGCCCAAGCCTACAGATCAGGTTGGGGAGGGTGGGTGTTTAAACCAACAGGCTTCTTTTTTCCCAACaaaacc                                                                                                                                                                                                     >  2:238523/1‑226 (MQ=255)
                         tGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACCCAGGTTGGGGCGGCGGGGGGTTTTAAACGATTGGCTTTTTTTTTGCCCACGGAggc                                                                                                                                                                                                     >  1:20728/1‑227 (MQ=255)
                              aCCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCCGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTTTGCCAAAACCTACGACACAGGTTGGGGCGGGGGGGGGTTTAAAACGATCGGCTTTTTTTACGCCAAAGAGAGtctccg                                                                                                                                                                                                 >  2:670947/1‑227 (MQ=255)
                                 gggAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCcgcg                                                                                                                                                                                             <  1:688115/229‑1 (MQ=255)
                                 gggAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTACCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTCCGACACAGGTTGGGGCGGGGTGGAGGTTAAAACGATCGGCTTTTTTATTGCCAACGaaattttgccccc                                                                                                                                                                                             >  1:640315/1‑218 (MQ=255)
                                           ccgccgAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGatcat                                                                                                                                                                                   <  1:379152/229‑1 (MQ=255)
                                             gccgAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGAAACAGGTTGGGGCGGGGTGGGGGTTTAAACGGTCGGGTTTTTTTTCTCCAAAGAAGTGACGCCGGGTTTTggataatc                                                                                                                                                                                 >  2:503481/1‑222 (MQ=255)
                                                                     gagggaggTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTAt                                                                                                                                                         <  1:509949/229‑1 (MQ=255)
                                                                         gAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACGCAGGTTGGGGCGGTGTGGGGTTTTAAACGGATGGCTTTTTTTTCGCCAAAGGAGGCTGGCCGCGTTTTGTTACTCTTGGGTACGAAAGTACCGGTTTTTTTGGc                                                                                                                                                     >  1:408629/1‑229 (MQ=255)
                                                                         gAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGCGTGGGGTTTTAAACGATCGGCTTTTTTATTGCCAACGGAGGCTCACCGCGTTTTTATCATCTGGTGAAAAAAAAAACAGGTTTTAACggg                                                                                                                                                     >  2:258680/1‑228 (MQ=255)
                                                                           gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGCTGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGGGTGGGGTTTTAAACAGTCGCCCTTTTTTTCGCCAAAGCAAGCTCCCCCGGTTTTTATCATCTGGTGGAAGAAGTAACCGGgttttacggtgt                                                                                                                                                   >  2:534669/1‑221 (MQ=255)
                                                                           gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTATAACGATTCGCCTTTTTTTCGCCAAAGAAGTCTTGCCGGGTTTTGATCATCTGGTGAAAGAAGATACCGGattttttggact                                                                                                                                                   >  1:674388/1‑221 (MQ=255)
                                                                           gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGGCTCGCCGCGTTTTTGTCATCTGGGGAAAGAAGATACCGGgttttttcgctt                                                                                                                                                   >  1:19690/1‑221 (MQ=255)
                                                                           gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGGTGGGGCGGTGTGGGGTTTAAAACGATTGGCTTTTTTATCTCCAACGAAGTGTTTGCGGGGTTTGGTCACCTGGGGAAAGAAAAAACAGGGTTTTTccggtg                                                                                                                                                   >  2:235984/1‑224 (MQ=255)
                                                                           gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGGAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGGGTGGTTTAAAACGATCGGCTTTTTTTTCGCCCACGAAGTCTCCCCGCGTTTTTATCACCTGGGGAAAAAAGAGACAGGgtttttacgctt                                                                                                                                                   >  2:383900/1‑221 (MQ=255)
                                                                                          cGAAAGATCTTTCGATTACTTTGTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCa                                                                                                                                   <  1:258680/229‑1 (MQ=255)
                                                                                               gATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGGGTGGGGTTTAAAACGATAGGCTTTTTTATCGCCAACGAAAGTTCGCCGCGGTTTTATCATTTGGGGAAAAAAGGTACCGGGTTTTTTGGCTTCTAAAAAATAGAGCaagaat                                                                                                                              >  2:252882/1‑224 (MQ=255)
                                                                                               gATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGGTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGGCACAGGTTGGGGCGGGTTGGTGTTTAAAACGATCCGCTTTTTTATCCCCAACGAAGGCTTGCCTCCTTTTTGTTATCTTGTGACGAAAAAAACCGGGTTTTTCCGCTTGAAAAataatggggagaat                                                                                                                               >  2:413884/1‑217 (MQ=255)
                                                                                               atttttgtgttttgtttttGCGGAGTGGAGTTTCCCAACCCGTTCTGCCGCGCTTCTGCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTg                                                                                                                              <  2:20728/215‑1 (MQ=255)
                                                                                                    ttCGATTACTTTTGGCGGCGTGAAGTTTCCCAACCCTTTCTGCCTCTCTTCGTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGaa                                                                                                                         <  1:717637/229‑1 (MQ=255)
                                                                                                      cGATTTTTTTTTGGGGCTGGAATTTTCCCAACCCGTTCGGCCTCTCTTCTGCGCCGGTAGGCAACTGCTATGAGATGTTTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACa                                                                                                                       <  2:561384/229‑1 (MQ=255)
                                                                                                           aCTTTTTGCGGCGTGAATTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATTTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTTTGGGGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGt                                                                                                                  <  2:557010/229‑1 (MQ=255)
                                                                                                           aCTTTTGGGGGCGTGAAGTTTCCCAACCCGTTCTGCCTGTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACTCAGGTTGGGGCGGTGTGGTGTGTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGt                                                                                                                  <  1:173075/229‑1 (MQ=255)
                                                                                                                                        gTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCgg                                                                                     <  1:444872/229‑1 (MQ=255)
                                                                                                                                        gTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGCGTGGTGATTAAAACGATCGGCTTTTTTATCGCCCAAGCAGTCTCGGCGCGGTTTGATCATCTCGTGAAAGAAGATACCGGATTTATCGGCTTCCAAAATATTGATGCGATTGGTTAAAAACCGTTGGAAGAGaaaatgagcaccacgatgcgg                                                                                     >  2:228664/1‑210 (MQ=255)
                                                                                                                                            tcctcctctgtctcGCCGGTAGGCAACTTGTATGAGTGGTGTGCCAAAGCCTACGACGAAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCGTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTAGCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTga                                                                                 <  2:640314/217‑1 (MQ=255)
                                                                                                                                                 ctctTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGGTTTATCGGCTTCAAAAATATGGAGCCGATTGCTGAACATCCGTTGGAAGAGAAACTGGCCGCCCTGCGTCCGCTgaagga                                                                            >  1:8096/1‑228 (MQ=255)
                                                                                                                                                 ctctTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGGTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTgaaggaa                                                                           >  1:86343/1‑229 (MQ=255)
                                                                                                                                                        tcGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGGTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAAAATCCGTTGGAAGAGAATCTGGCCGCCCTCCGTCGGCTGAAGGAAGATTatc                                                                    >  1:462814/1‑227 (MQ=255)
                                                                                                                                                         cggcggtagggcatttatATGAGATGTGTGGCAAAGCCTACGACTAAGGTGGGGGCGGTTGGGTGTTTAAAACGATCGGCTGTTTTATCGCCAACGAAGTCTCGCCGCGGTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTAccc                                                                   <  1:334053/212‑1 (MQ=255)
                                                                                                                                                           ccGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGGTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTTATCATCTGGTGAACGAAGATCACGGTTTTATCCGCTTCCAAAATAAGGGCGCGATTGCTGTACATCAGTTTGAGGGGAATTTGGGCCACCGGCGCCGGATGTAGAAAGagatccccg                                                                 >  2:334053/1‑221 (MQ=255)
                                                                                                                                                             ggTTGGCAACTGCTTTGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGGGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGAc                                                               <  2:198931/229‑1 (MQ=255)
                                                                                                                                                              gttggCAACTGTTTTGAGATGTGTGCCAAAGCCTACGAACCAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTAGCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACa                                                              <  2:19690/226‑1 (MQ=255)
                                                                                                                                                              gTAGGCAACTGCTGGGAGATGTGGTCCAAAGCCTAAGACACAGGTTGGGGCGGTTTGGTGTTTAAAACGATCGGCTTTTTTAGCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACa                                                              <  2:489006/229‑1 (MQ=255)
                                                                                                                                                              gTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCCGAGTGCTGAAAATCCGTTGGGAAGGAAACTGGACCCCCTGGCTGGGCTGAAGGCAGATTACCCGGAc                                                               >  2:564527/1‑228 (MQ=255)
                                                                                                                                                              gTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACa                                                              <  1:353980/229‑1 (MQ=255)
                                                                                                                                                                tggCAATTGCTTTTAGTTGTGTGCCAAAGCCCACGACACGGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACaaa                                                            <  2:86343/228‑1 (MQ=255)
                                                                                                                                                                            tgtgattggtgCCAAGGCCTTCGACACAGGTTGGGGCGGTGTGGTGGTTAAAACGATCGGCTTTTTGATCGCCAACGAAGTCTCGCCGCGTTTTGATCAGCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCt                                              <  2:408629/221‑1 (MQ=255)
                                                                                                                                                                              agaTGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGCAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAACAGATTTGTGAAAACGCGTTGGAAGGAAACTTGGCCGCCCTGCAGCGGCTGGAGGGAAGTTACCCGGGAAAAGAATTTGATCGCtt                                             >  2:454397/1‑229 (MQ=255)
                                                                                                                                                                               gaTGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTc                                            <  1:25060/228‑1 (MQ=255)
                                                                                                                                                                                  tttgcccAAGGCTTTCGAAACTGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATc                                       <  2:307027/224‑1 (MQ=255)
                                                                                                                                                                                           aGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATTGAGCAGATTGCTGAACATCCCTTGGAAGGGAATCTGGCCGCCCTGCGTCGGCTGAGGGAAGATTACCCGGGCAAAAAATTTGACGCTTCCGTAATGGGGGaa                              >  2:120873/1‑229 (MQ=255)
                                                                                                                                                                                                aCGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGCTACCGGGTTTATCGGCTTCCAAAAAAATGGACAGATTGCTGAACATCCGTTGGAAGGGGATATGGCCGCCCAGCCTCGGGTGAAGGAAGATTCGCCGGGCCAAGTGTTGTCGCCTTCGGGCATGGGGGGAAAAGa                         >  2:636506/1‑229 (MQ=255)
                                                                                                                                                                                                aCGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGGGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATTGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTAACCGGACAAAGGATTGATACCTTCGATCATGGGGGaaacaga                         >  1:105231/1‑225 (MQ=255)
                                                                                                                                                                                                  gtctcaGGTTGGGGGGGTGTGGGGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGag                        <  1:248480/224‑1 (MQ=255)
                                                                                                                                                                                                   acacAGGTTGGGGCTGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGagc                       <  1:534669/228‑1 (MQ=255)
                                                                                                                                                                                                               ggCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCTAAAATATGGAGCAGATTGGTGAACACCCGTTTGAAGGAAAACTGGCCCCCCTGCCTCGGCTCAGAGAAGGATTCCCGGGACAAAGATTGATGCCCTCGACCCTTGGGGCAAAATgcaagaaagaggcg             >  2:182607/1‑215 (MQ=255)
                                                                                                                                                                                                                    tgtggtggTTAAAACGATCGGCTTTTTTATCGCCAACGAAGGCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGCTTGATGAACACTCGTTGGAAGAGAATCTGGCCGGCCTGCTGCGGCTGGAGGAAAATTACCCGGACAAAATATTGATCCGTTTGCTCCTGGGGGGAAATGAGCAGGCAAGGGAGGAGCTg      >  2:245635/1‑229 (MQ=255)
                                                                                                                                                                                                                        gtgtTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGCGCTGgcgc  >  1:368186/1‑229 (MQ=255)
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                         
GGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTTGGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGAAGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTTCAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGAAGGAAGATTACCCGGACAAAGTATTGATCGCTTCGATCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGC  >  NC_000913/2235180‑2235623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: