| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,235,396 | T→G | 21.7% | V45G (GTG→GGG) | preA → | dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,235,396 | 0 | T | G | 21.7% | 78.8 / 3.4 | 57 | V45G (GTG→GGG) | preA | dihydropyrimidine dehydrogenase, NADH‑dependent, subunit C |
| Reads supporting (aligned to +/- strand): ref base T (19/21); new base G (13/2); total (34/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.30e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTCGA‑TCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGC > NC_000913/2235180‑2235623 | ggTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGGAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCCACTGCTATTGGATGGGTGCCAAAGCCTACGACACCGGTT‑GGGGCGGGTGGGAGTTTTAAACGAt > 1:127864/1‑229 (MQ=255)ggTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTTTGCCTCTCTTCTTCGCCGGTAGGCCACTGCTATGAGTTGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGGGTGTGGTTTTAAACGag > 1:489006/1‑228 (MQ=255)ggTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGGAACTGCTATGAGATGTGTGCCACAGCCTACGAACCCGGTT‑TGGGCGGGGGGGGGTTTAAAAAGaa > 2:717636/1‑228 (MQ=255)ggTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACGATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAGGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCATTTAGATGTTTGCCAAACCCTACGACCCGGGTTGGGGGGGGTGGGGTGTTAAAAAcaa > 2:104239/1‑227 (MQ=255) gACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTCTGAGATGTGTGCCAAAGCCTACGAACCAGGTT‑GGGGCGGGGTGGGGGTTAAAACGATCgg > 2:25060/1‑229 (MQ=255) aaaaaCCGTGGTCGAGGCCGTGAAACCCGGGAAAGAAGCCGCCGAGGCGATTCCTCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCt < 1:589793/228‑1 (MQ=255) tGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCCATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGGGGGGTTTAAAACGAACGGCTTTTTTAtt > 2:444872/1‑228 (MQ=255) ggTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTTAAACGATCGGCTTTTTTATc > 1:706372/1‑228 (MQ=255) tGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGCGGGTTTGGTGTTTAAAACGATCGGCTTTTTTTTCGCCAAc > 1:561385/1‑228 (MQ=255) tGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTATAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCTCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGCCACAGGGT‑GGGGCGGGGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGC‑AGtc > 1:198931/1‑229 (MQ=255) tGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAACGATCTTTCGCTTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTATTCGCCGGTCGGCAACTGCTATGAGATGGTTGCCCAAGCCTACAGATCAGGTT‑GGGGAGGGTGGGTGTTTAAACCAACAGGCTTCTTTTTTCCCAACaa‑aacc > 2:238523/1‑226 (MQ=255) tGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACCCAGGTT‑GGGGCGGCGGGGGGTTTTAAACGATTGGCTTTTTTTTTGCCCACGG‑Aggc > 1:20728/1‑227 (MQ=255) aCCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCCGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTTTGCCAAAACCTACGACACAGGTT‑GGGGCGGGGGGGGGTTTAAAACGATCGGCTTTTTTTACGCCAAAGAGAGtctccg > 2:670947/1‑227 (MQ=255) gggAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCcgcg < 1:688115/229‑1 (MQ=255) gggAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTACCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTCCGACACAGGTT‑GGGGCGGGGTGGAGGTTAAAACGATCGGCTTTTTTATTGCCAACGa‑aattttgccccc > 1:640315/1‑218 (MQ=255) ccgccgAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGatcat < 1:379152/229‑1 (MQ=255) gccgAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGAAACAGGTT‑GGGGCGGGGTGGGGGTTTAAACGGTCGGGTTTTTTTTCTCCAAAGA‑AGTGACGCCGGGTTTTggataatc > 2:503481/1‑222 (MQ=255) gagggaggTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTAt < 1:509949/229‑1 (MQ=255) gAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACGCAGGTT‑GGGGCGGTGTGGGGTTTTAAACGGATGGCTTTTTTTTCGCCAAAGG‑AGGCTGGCCGCGTTTTGTTACTCTTGGGTACGAAAGTACCGGTTTTTTTGGc > 1:408629/1‑229 (MQ=255) gAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGCGTGGGGTTTTAAACGATCGGCTTTTTTATTGCCAACGG‑AGGCTCACCGCGTTTTTATCATCTGGTGAAAAAAAAAACAGGTTTTAACggg > 2:258680/1‑228 (MQ=255) gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGCTGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGGGTGGGGTTTTAAACAGTCGCCCTTTTTTTCGCCAAAGC‑AAGCTCCCCCGGTTTTTATCATCTGGTGGAAGAAGTAACCGGgttttacggtgt > 2:534669/1‑221 (MQ=255) gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTATAACGATTCGCCTTTTTTTCGCCAAAGA‑AGTCTTGCCGGGTTTTGATCATCTGGTGAAAGAAGATACCGGattttttggact > 1:674388/1‑221 (MQ=255) gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGGCTCGCCGCGTTTTTGTCATCTGGGGAAAGAAGATACCGGgttttttcgctt > 1:19690/1‑221 (MQ=255) gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGGT‑GGGGCGGTGTGGGGTTTAAAACGATTGGCTTTTTTATCTCCAACGA‑AGTGTTTGCGGGGTTTGGTCACCTGGGGAAAGAAAAAACAGGGTTTTTccggtg > 2:235984/1‑224 (MQ=255) gCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGGAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGGGTGGTTTAAAACGATCGGCTTTTTTTTCGCCCACGA‑AGTCTCCCCGCGTTTTTATCACCTGGGGAAAAAAGAGACAGGgtttttacgctt > 2:383900/1‑221 (MQ=255) cGAAAGATCTTTCGATTACTTTGTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCa < 1:258680/229‑1 (MQ=255) gATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGGGTGGGGTTTAAAACGATAGGCTTTTTTATCGCCAACGA‑AAGTTCGCCGCGGTTTTATCATTTGGGGAAAAAAGGTACCGGGTTTTTTGGCTT‑CTAAAAAATAGAGCaagaat > 2:252882/1‑224 (MQ=255) gATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGGTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGGCACAGGTT‑GGGGCGGGTTGGTGTTTAAAACGATCCGCTTTTTTATCCCCAACGA‑AGGCTTGCCTCCTTTTTGTTATCTTGTGACGAAAAAAACCGGGTTTTTCCGCTT‑GAAAAataatggggagaat > 2:413884/1‑217 (MQ=255) atttttgtgttttgtttttGCGGAGTGGAGTTTCCCAACCCGTTCTGCCGCGCTTCTGCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTg < 2:20728/215‑1 (MQ=255) ttCGATTACTTTTGGCGGCGTGAAGTTTCCCAACCCTTTCTGCCTCTCTTCGTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGaa < 1:717637/229‑1 (MQ=255) cGATTTTTTTTTGGGGCTGGAATTTTCCCAACCCGTTCGGCCTCTCTTCTGCGCCGGTAGGCAACTGCTATGAGATGTTTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACa < 2:561384/229‑1 (MQ=255) aCTTTTTGCGGCGTGAATTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATTTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTTTGGGGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGt < 2:557010/229‑1 (MQ=255) aCTTTTGGGGGCGTGAAGTTTCCCAACCCGTTCTGCCTGTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACTCAGGTT‑GGGGCGGTGTGGTGTGTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGt < 1:173075/229‑1 (MQ=255) gTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCgg < 1:444872/229‑1 (MQ=255) gTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGCGTGGTGATTAAAACGATCGGCTTTTTTATCGCCCAAGC‑AGTCTCGGCGCGGTTTGATCATCTCGTGAAAGAAGATACCGGATTTATCGGCTT‑CCAAAATATTGATGCGATTGGTTAAAAACCGTTGGAAGAGaaaatgagcaccacgatgcgg > 2:228664/1‑210 (MQ=255) tcctcctctgtctcGCCGGTAGGCAACTTGTATGAGTGGTGTGCCAAAGCCTACGACGAAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCGTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTAGCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTga < 2:640314/217‑1 (MQ=255) ctctTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGGTTTATCGGCTT‑CAAAAATATGGAGCCGATTGCTGAACATCCGTTGGAAGAGAAACTGGCCGCCCTGCGTCCGCTga‑agga > 1:8096/1‑228 (MQ=255) ctctTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGGTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTga‑aggaa > 1:86343/1‑229 (MQ=255) tcGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGGTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAAAATCCGTTGGAAGAGAATCTGGCCGCCCTCCGTCGGCTGA‑AGGAAGATTatc > 1:462814/1‑227 (MQ=255) cggcggtagggcatttatATGAGATGTGTGGCAAAGCCTACGACTAAGGTG‑GGGGCGGTTGGGTGTTTAAAACGATCGGCTGTTTTATCGCCAACGA‑AGTCTCGCCGCGGTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTAccc < 1:334053/212‑1 (MQ=255) ccGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGGTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTTATCATCTGGTGAACGAAGATCACGGTTTTATCCGCTT‑CCAAAATAAGGGCGCGATTGCTGTACATCAGTTTGAGGGGAATTTGGGCCACCGGCGCCGGATGT‑AGAAAGagatccccg > 2:334053/1‑221 (MQ=255) ggTTGGCAACTGCTTTGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGGGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGAc < 2:198931/229‑1 (MQ=255) gttggCAACTGTTTTGAGATGTGTGCCAAAGCCTACGAACCAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTAGCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACa < 2:19690/226‑1 (MQ=255) gTAGGCAACTGCTGGGAGATGTGGTCCAAAGCCTAAGACACAGGTT‑GGGGCGGTTTGGTGTTTAAAACGATCGGCTTTTTTAGCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACa < 2:489006/229‑1 (MQ=255) gTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCCGAGTGCTGAAAATCCGTTGGGAAGGAAACTGGACCCCCTGGCTGGGCTGA‑AGGCAGATTACCCGGAc > 2:564527/1‑228 (MQ=255) gTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACa < 1:353980/229‑1 (MQ=255) tggCAATTGCTTTTAGTTGTGTGCCAAAGCCCACGACACGGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACaaa < 2:86343/228‑1 (MQ=255) tgtgattggtgCCAAGGCCTTCGACACAGGTT‑GGGGCGGTGTGGTGGTTAAAACGATCGGCTTTTTGATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCAGCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑Ct < 2:408629/221‑1 (MQ=255) agaTGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGCAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAACAGATTTGTGAAAACGCGTTGGAAGGAAACTTGGCCGCCCTGCAGCGGCTGG‑AGGGAAGTTACCCGGGAAAAGAATTTGATCG‑Ctt > 2:454397/1‑229 (MQ=255) gaTGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTc < 1:25060/228‑1 (MQ=255) tttgcccAAGGCTTTCGAAACTGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTCGA‑Tc < 2:307027/224‑1 (MQ=255) aGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATTGAGCAGATTGCTGAACATCCCTTGGAAGGGAATCTGGCCGCCCTGCGTCGGCTGA‑GGGAAGATTACCCGGGCAAAAAA‑TTTGACG‑CTTCCG‑TAATGGGGGaa > 2:120873/1‑229 (MQ=255) aCGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGCTACCGGGTTTATCGGCTTCCAAAAAAATGGA‑CAGATTGCTGAACATCCGTTGGAAGGGGATATGGCCGCCCAGCCTCGGGTGA‑AGGAAGATTCGCCGGGCCAAGTG‑TTG‑TCGCCTTCGG‑GCATGGGGGGAAAAGa > 2:636506/1‑229 (MQ=255) aCGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGGGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATTGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTAACCGGACAAAGGA‑TTGATAC‑CTTCGA‑TCATGGGGGaaacaga > 1:105231/1‑225 (MQ=255) gtctcaGGTT‑GGGGGGGTGTGGGGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTCGA‑TCATGGGGGAAAATGag < 1:248480/224‑1 (MQ=255) acacAGGTT‑GGGGCTGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTCGA‑TCATGGGGGAAAATGagc < 1:534669/228‑1 (MQ=255) ggCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CTAAAATATGGAGCAGATTGGTGAACACCCGTTTGAAGGAAAACTGGCCCCCCTGCCTCGGCTCAGAGAAGGATTCCCGGGACAAAG‑A‑TTGATGC‑CCTCGACCCTTGGGGCAAAATgcaagaaagaggcg > 2:182607/1‑215 (MQ=255) tgtggtggTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGGCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGCTTGATGAACACTCGTTGGAAGAGAATCTGGCCGGCCTGCTGCGGCTGG‑AGGAAAATTACCCGGACAAAATA‑TTGATCC‑GTTTGC‑TCCTGGGGGGAAATGAGCAGGCAAGGGAGGAGCTg > 2:245635/1‑229 (MQ=255) gtgtTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTCGA‑TCATGGGGGAAAATGAGCAGCAATGGGAGGCGCTGgcgc > 1:368186/1‑229 (MQ=255) | GGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAGGCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAACGAAAGATCTTTCGATTACTTTTTGCGGCGTGAAGTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGCTATGAGATGTGTGCCAAAGCCTACGACACAGGTT‑GGGGCGGTGTGGTGTTTAAAACGATCGGCTTTTTTATCGCCAACGA‑AGTCTCGCCGCGTTTTGATCATCTGGTGAAAGAAGATACCGGTTTTATCGGCTT‑CAAAAATATGGAGCAGATTGCTGAACATCCGTTGGAAGAGAATCTGGCCGCCCTGCGTCGGCTGA‑AGGAAGATTACCCGGACAAAGTA‑TTGATCG‑CTTCGA‑TCATGGGGGAAAATGAGCAGCAATGGGAGGAGCTGGCGC > NC_000913/2235180‑2235623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |