Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,541,881 A→C 19.1% G210G (GGT→GGG cysU ← sulfate/thiosulfate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,541,8810AC19.1% 105.1 / 2.8 55G210G (GGT→GGGcysUsulfate/thiosulfate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (21/21);  new base C (5/8);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 5.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACGCACCACAAACGGAATGCTGGTAAAGGCCATAGCCACCGCAATCCCCAGCC  >  NC_000913/2541655‑2542092
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                    
ttCAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCCTCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGCTAAAAATAACCGCGCCAAATGCAcc                                                                                                                                                                                                                    >  1:750594/1‑229 (MQ=255)
 tCAATTGGGTAACTTCCGGCATTAATGACCTAACACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCAcc                                                                                                                                                                                                                    <  1:362779/228‑1 (MQ=255)
      tGGTTATCTTCCGCCATTAATGCCCTCCCACCCGCCGACAAAAGCTACTTTGCAGAGTGTTAATTGAGAACAGCAGCGGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGGCt                                                                                                                                                                                                             <  2:687281/229‑1 (MQ=255)
      tGGGTAACTTCCGCCATTAAGGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGACGCCGCGAGGATAACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACt                                                                                                                                                                                                             <  1:293221/229‑1 (MQ=255)
        ggTACCTCCCGGCATTAATGACCTCCCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTAc                                                                                                                                                                                                           <  1:61272/229‑1 (MQ=255)
        ggTAATTGCCGCAATTAATGACCGACCACACGACGACCAAAGCGACTTTGGAGAGTGTTAATTGAGAACAGCAGCAGCAGGGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTAc                                                                                                                                                                                                           <  1:673010/229‑1 (MQ=255)
                   ccATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGa                                                                                                                                                                                                 >  1:686907/1‑228 (MQ=255)
                              cTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCGGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGCCTTCCACGCGCTATTTCCGGGGAAAAAAATCACCGCGGCAAATTCACCCAGCCCACGGGTAAAAGACAGCACCAcacc                                                                                                                                                                                     >  2:692913/1‑229 (MQ=255)
                                aCCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCCAACTCCTGTAAGCGCACAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCAcgcccgc                                                                                                                                                                                  >  2:7383/1‑229 (MQ=255)
                                aCCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGCTATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCAcgccc                                                                                                                                                                                    >  1:200451/1‑228 (MQ=255)
                                aCCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCCCCAAGACTACGGGAAAACGCAAGCGACAcccccg                                                                                                                                                                                   >  2:178046/1‑229 (MQ=255)
                                       gCCGACCAAAGCGACTTTGCAGACTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCCCCAAGACTACGGGTAAACGCCAGCGCCACGCCCCccacaac                                                                                                                                                                            >  1:173018/1‑226 (MQ=255)
                                        ccGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAg                                                                                                                                                                            >  1:257369/1‑228 (MQ=255)
                                           aCCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCCCCAAGACTACGGGTAAACGACACAGCAACCCCCCccaccccccc                                                                                                                                                                         >  2:605874/1‑223 (MQ=255)
                                                       ttacAGAGGGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATGGCGCTCGCGGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTcc                                                                                                                                                            <  1:182145/226‑1 (MQ=255)
                                                         gCAGATTGTTAATTGAGAACGAGCGAGCGAGGAATGGCGGTAGGATCACCGAACCACGCGCGCTCGCCGCCGGGTAATCCACCTCCGTAAAGCGCACAAAAATCAGAAGCGCCGTCCCTTCCGTCTTCCACGCGATATGTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCgg                                                                                                                                                          <  2:272349/229‑1 (MQ=255)
                                                            aggttttAATTGGAAACAGCAGCAGCAGAGATGCCCAGAGGTCCCCCGAAGAAATCGCGCTCGGTGCCGGGTAATCAAACTCCAGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGCTTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCgg                                                                                                                                                          <  2:340698/221‑1 (MQ=255)
                                                               tgtTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATCAAAATCACCGCGCCAAATTCCCCAAGACTACGGGTAAACACAAACCCCACGCCCCCCCCCACCCCCGGAAAAAGCCCCGCCAacac                                                                                                                                                    >  2:355203/1‑229 (MQ=255)
                                                               tgtTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCACGACTACGGGGACACGAAAGCGCCACGCCCGCCACCACCGCCGGCGAAAGCTCCgcaacaac                                                                                                                                                    >  1:308886/1‑222 (MQ=255)
                                                               tgtTAATTGAGAACAGCAGCAGCAGAAAAGCCGCAAGGATCACCGAAGCAATCGCGCTCGCTGCCGGTTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGcac                                                                                                                                                    <  1:283854/229‑1 (MQ=255)
                                                                         gaACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAAGGACAGCGCCAcgccc                                                                                                                                                                                    >  2:300774/1‑187 (MQ=255)
                                                                         gaACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCAcgccc                                                                                                                                                                                    <  1:300774/187‑1 (MQ=255)
                                                                           aCAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCCCCGGCAAAAACCCCGGACGCACCACTTTCCAGaa                                                                                                                                        >  2:344916/1‑229 (MQ=255)
                                                                            caccagcggcagGGATGCCGCGAGGATCACCGAAGCAATCGGGCTCGCTGCCGGGTAATCGAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTCCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGaaa                                                                                                                                       <  2:509458/226‑1 (MQ=255)
                                                                               cagcagcagCGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTGACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCCCGCCAAATTCCCCAAAACCACGGGTAAAAGACAGCGCCACGCCCCCCCCCAACGCCGGAACAAGCCCCGgcccgcccaccttcacaaacaac                                                                                                                                    >  2:390426/1‑208 (MQ=255)
                                                                               cagcagcagAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAACTCACCGCGCCAAATTCACCCAGACTACGGGTAAACGACAGCGACACGCCCGCCCCCACCACCGGAGAAAGCCCCGGCCCCACCACttttccacaactc                                                                                                                                    >  2:29461/1‑219 (MQ=255)
                                                                                     gcagAGATTCCACGGAGATCCCCCAAGCACACTCCGTCGCTGCCAGTAAACCAAACCCCTTCAAGCGCAGAAAAATCATCAGGCACGGCACTTCCGTTCTCCACCGGATAATTCCGGCGATAAAAATCACCGCGCATAATCCCCTAAGAGTACGGTTAAACGACAGCGCCACGACCGCCACCAGCGTCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAACCTCTGCCa                                                                                                                               <  2:427553/229‑1 (MQ=255)
                                                                                            aTGCCGCGAGGAGCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTCCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGCCTACGGGTGAACGACAGCGCCACGCCCGCCACCCCAGTCGGGGAAAGCTCAGGAAGCGCCACTGTGAGGACACTCTCCCcgcgggg                                                                                                                         >  2:489881/1‑226 (MQ=255)
                                                                                                          cccGAAGCCATCGCCCTCGCTGCCCGGTAATCAAACTACTGTAAGCCCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGtt                                                                                                            <  1:702219/227‑1 (MQ=255)
                                                                                                               aGCAACCGCGCTCGTCGCCGGGTAATCAAACTCCTGGAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTccgc                                                                                                       <  1:707011/228‑1 (MQ=255)
                                                                                                                   accgcgcccgcgccCGGGTACTCAAACTCCTGTAAGCCCTCAAAAATAATCAGCAGCGCCCCTTCCTTCTTCCACGCGATATTTCCGGCAATAAAAATCCCCGCGCCAAATTCCCCAAGACTGCGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGct                                                                                                   <  2:418759/226‑1 (MQ=255)
                                                                                                                   aTCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCATCGCGTTGCACCAAGCGATTCcgccgcg                                                                                                   >  1:258686/1‑227 (MQ=255)
                                                                                                                    tCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGcttc                                                                                                 >  1:643368/1‑229 (MQ=255)
                                                                                                                    tCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCAAGCCAGCCACCAGCGCCGGCGAAAGCTCCGCCAGCACCACTTTCCAAAAACCCTTCACACGCGTTGAAACaacagataccaccactct                                                                                                 >  2:12226/1‑213 (MQ=255)
                                                                                                                        gctcgctGCCCGGTAATCAAACTCCTGTAAGCGCACAAAAACCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTGCCGGCGATAAAAACCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCa                                                                                             <  1:344916/229‑1 (MQ=255)
                                                                                                                                                   tAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCATCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAACCGTTTCCGCCGCTTCTCCATATTCCGGGCCTAACCCTTCCAGCAcc                                                                  >  1:485541/1‑229 (MQ=255)
                                                                                                                                                     accccaaaaaaaCCATCAGCGACGTCACTTCCGTCTCCCACGCGATATTCCCGCCGAAAAAAATCCCCGCCCCAAATGCACCAAGACTACGGGTAAACGCCAGCGCCCCGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCgg                                                                <  2:643367/222‑1 (MQ=255)
                                                                                                                                                     aGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCgg                                                                <  1:178046/229‑1 (MQ=255)
                                                                                                                                                       ctcaaaaaaaTCATCCCCGCCCTCCCTCCCCTCCTCCACCGGAATTTTCCGGCGAAAAAACTCCCCGCCCCAAATTCCCCAAGCATACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCt                                                              <  2:12408/224‑1 (MQ=255)
                                                                                                                                                        gcACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCGAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGCAAGCTCCGGCCGCACCACTTTGCAGAAACTCTGCCCGCGCGTTGACCCAACCGTTTCCGCCGCTTATTCCACTCCCGGGGCAAACCCTTCCAACAACGGcg                                                              >  2:319201/1‑227 (MQ=255)
                                                                                                                                                                  catcaGCGACGCCACTTCCGTCTCCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACg                                                    <  1:458746/228‑1 (MQ=255)
                                                                                                                                                                  aatttgttataaaggacaggTCTCCCACGGAATATTTCCGGCGAAAAAAACCCCCGCCCCAAATTCCCCAAGCCCACGGGTAAACGCCAGCGCCACGCCCGCCCCCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACGc                                                   <  2:192840/210‑1 (MQ=255)
                                                                                                                                                                                      tCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACGCACCACAAACGGACGGCTgt                                >  1:701751/1‑227 (MQ=255)
                                                                                                                                                                                         tCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAAc                                                                              <  1:96005/179‑1 (MQ=255)
                                                                                                                                                                                         tCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAAc                                                                              >  2:96005/1‑179 (MQ=255)
                                                                                                                                                                                             cgcgATTTTCCCGGCGAAAAAACTCACCGCGCCAAATTCCCCAAGCCTCCGGGTAACCGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACGCACCACAAACGGAATGCTGGTAAAGGcc                        <  1:605875/229‑1 (MQ=255)
                                                                                                                                                                                                gATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACGc                                                   >  1:192840/1‑199 (MQ=255)
                                                                                                                                                                                                          gCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACGCACCACAAACGGAATGCTGTTAAAGGCCATAGCAACCGCaa           >  1:710677/1‑229 (MQ=255)
                                                                                                                                                                                                              tAAAAATCACCGCGCAAAATGCACCAAGCCCACGGTTAACCGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACGCACCACAAACGGAATGCTGGTAAAGGCCATAGCCACCGCAATccc       <  1:319201/229‑1 (MQ=255)
                                                                                                                                                                                                                 agaccaccggccaaaaTTCCCCAAGTCTACGGGTAAACGACAGCCCCACGCCCGGCCCCCGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACGCACCACAAACGGAATGCTGGTAAAGGCCATAGCCACCGCAATCCCCAg    <  1:692914/216‑1 (MQ=255)
                                                                                                                                                                                                                 aagccACCGCGCCAAATTCCCCAAGCACTCGGGAACACGACCGCGCCCAGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACGCACCACAAACGGAATGCTGGTAAAGGCCATAGCCACCGCAATCCCCAg    <  1:220179/225‑1 (MQ=255)
                                                                                                                                                                                                                 aactCACCGGCCAAAATTCCCCAGGCCTACGGGTAACCGAAAACCCCCCGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCCCCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACGCACCACAAACGGAATGCTGGTAAAGGCCATAGCCACCGCAATCCCCAg    <  1:12226/226‑1 (MQ=255)
                                                                                                                                                                                                                 aaagccccGCGCCAAATCCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCCCCGGCAGCACCACTTTGCAGAACCTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACGCACCACAAACGGAATGCTGGTAAAGGCCATAGCCACCGCAATCCCCAg    <  1:355203/223‑1 (MQ=255)
                                                                                                                                                                                                                   aacccccccccAAATTCACCAAGAGTACGGGAAAACGCCACCGCCACGCCCGCCACCAGCGCCGGAGAAAGCCCCGGCAGCACCACTTGGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACGCACCACAAACGGAATGCTGGTAAAGGCCATAGCCACCGCAATCCCCAGcc  <  2:686906/220‑1 (MQ=255)
                                                                                                                                                                                                                   aTCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACg                                                    >  1:385476/1‑179 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                    
TTCAATTGGGTAACTTCCGCCATTAATGACCTACCACACGCCGACCAAAGCGACTTTGCAGAGTGTTAATTGAGAACAGCAGCAGCAGAGATGCCGCGAGGATCACCGAAGCAATCGCGCTCGCTGCCGGGTAATCAAACTCCTGTAAGCGCACAAAAATCATCAGCGACGTCACTTCCGTCTTCCACGCGATATTTCCGGCGATAAAAATCACCGCGCCAAATTCACCAAGACTACGGGTAAACGACAGCGCCACGCCCGCCACCAGCGCCGGAGAAAGCTCCGGCAGCACCACTTTGCAGAAACTCTGCCAGCGCGTTGCACCAAGCGTTTCCGCCGCTTCTTCATATTCCGGGCCTAACTCTTCCAGCACCGGCTGCACGGTACGCACCACAAACGGAATGCTGGTAAAGGCCATAGCCACCGCAATCCCCAGCC  >  NC_000913/2541655‑2542092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: