Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,820,019	T→C	20.9%	E664G (GAG→GGG)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,820,019	0	T	C	20.9%	122.4 / 2.6	65	E664G (GAG→GGG)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (33/15);  new base C (11/4);  total (46/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACC  >  NC_000913/2819805‑2820246                                                                                                                                                                                                                        |                                                                                                                                                                                                                                    tACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAAACCGGAAGAGaaaaag                                                                                                                                                                                                                       >  2:44882/1‑226 (MQ=255)      aGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGCACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACCCGGATGAGAAATCCCCCa                                                                                                                                                                                                                   >  1:40318/1‑228 (MQ=255)      aGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGCTTCAGAGCTGCTGCGAAACAGCCCACTATCACCC‑GTCCGGCTGGCGTGAGTCACCCCCCCCACCCCGGTAGAGAAATCccccca                                                                                                                                                                                                                  >  2:280889/1‑227 (MQ=255)      aGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGCTGATGCGGAACAGACCAAACTCACCA‑GTGCGGCTGGCGTGAGTACAGCCACCAAACACGGAAGAGAAATAGCCCaa                                                                                                                                                                                                                  >  2:506798/1‑228 (MQ=255)      aGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCGACACAACACGGTAGAGAAATAGCCCAt                                                                                                                                                                                                                  >  1:660177/1‑229 (MQ=255)      aGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACCCAACCCGGTAGAGAAATCGCCCa                                                                                                                                                                                                                   >  1:156950/1‑228 (MQ=255)      aGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACACACATCACCA‑GCACGCCTGGCCGGAGAACCGACACACAACCCCGCAGAAAAAGCAcccca                                                                                                                                                                                                                  >  2:90774/1‑227 (MQ=255)           aCTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGTTGCGGAACAGCCCAATATCACCC‑GTGCGGCCGGCGGGAGGACCGCAACCCAACCCGGTAGAGaaacacccccggccca                                                                                                                                                                                                             >  2:451413/1‑216 (MQ=255)           aCTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGCCCAATATCACCA‑GTGCGGCTGGCGTGCGTACCGCCACACCACTCGGTAGAGAAATCGCCCATGCTc                                                                                                                                                                                                              >  1:18612/1‑228 (MQ=255)           aCTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCa                                                                                                                                                                                                             >  1:119192/1‑229 (MQ=255)                aTCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGGGAAATCGCCCATGCTCAGCAc                                                                                                                                                                                                         >  1:536495/1‑228 (MQ=255)                         attattGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGCACGCCTGCAGCAGTACCCGCTTCAGAGCTGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGCGTACCCCCACACATCTCGGTAGAGAAATCGCCCATGCTCCGCACCCGAACgcgc                                                                                                                                                                                               >  1:717175/1‑229 (MQ=255)                         attattGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGCTTACGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGGGAGGCACTCCACACAACCCGGTAAAAACACTCCCCATTCTCAACAcgccgaacc                                                                                                                                                                                                 >  2:106846/1‑221 (MQ=255)                         attattGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTAACCGATTCCGAGATGATGCGGACCAGACCAATATCACCA‑GTGCGGCTGGCGGGGGGACCCCCACACAACCCGTTAGAAAAATCGCCCCTGCCCacacccccacaccc                                                                                                                                                                                                >  2:170255/1‑215 (MQ=255)                                  aTCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCTGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGGGAGTACCGCCACACACCTCGGGAAGGAAATCGCCCATGCTCAGCACGCGTaaacaccccaaaaaa                                                                                                                                                                                      >  2:122653/1‑215 (MQ=255)                                   tCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACCCGGTCGAGAAATCGCCCATGCCCAGCACGCCGACGCGCCCATCATAc                                                                                                                                                                                      >  2:519338/1‑228 (MQ=255)                                   tCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGGACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACCCGGGAGAGAACACGCCCATGCTCAGAACGCGTACGCGCTACCCATACt                                                                                                                                                                                     >  1:217721/1‑229 (MQ=255)                                   cccccTTTCAGGAGGTGGGCGCCTTCCCTAACGCGAGCGGTGTTGGCATGAACGGGGGCGATAGCCCCTTCCCCGGTTACCGTCTCGATACGCCGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGCCCAATACCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACt                                                                                                                                                                                     <  2:210726/226‑1 (MQ=255)                                        tttCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACCCAACCCGGTAGAGAAATCGCCCATCCTCACACCGCGTCCGCGCTCACCCAACTtccc                                                                                                                                                                                 >  2:308504/1‑226 (MQ=255)                                          tCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGCTGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGGGAAATCGCCCACGCCCAGCACGCGAACGCCCccatcacaatttcccc                                                                                                                                                                               >  2:216439/1‑218 (MQ=255)                                                 aTGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCCCAGCACCCGTACGCGCTCCCCAAACTTCTCGCCGAACAg                                                                                                                                                                        >  2:737766/1‑228 (MQ=255)                                                            cGCTTAAGCGAGCGCTGTCTGCATGAACGGGGGCGATAGCCCCTTCCCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCa                                                                                                                                                              <  1:519338/227‑1 (MQ=255)                                                                             tCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTATAGAAATCGCCCATGCTCAGCACCCGTACGCGCTCACCATACTTCTCCCCGAACAGCGCCATCCCACCCTTCCCTTTCCCCGCtt                                                                                                                                           >  2:426374/1‑229 (MQ=255)                                                                             tCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATGCCACCTTTGCCTTTAGCCGCtt                                                                                                                                           >  1:180712/1‑229 (MQ=255)                                                                                                      cTTCCCCGGTTACCGCTCCGATACGACGAACGCCTGCAGCAGTACCGGATTCAGAGATGATGCGGAACAGACCAATATCCCCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGa                                                                                                                   <  1:170255/228‑1 (MQ=255)                                                                                                              gTTCCCGTTTCGATACAACGAACGCCGGCAGCAGTACCCGATTCAGGTATGAGGCGGACTAGACCAATATCCCCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAAt                                                                                                          <  1:737767/229‑1 (MQ=255)                                                                                                                   cGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAAAAGCGCCATCGCCCCTTTCCCTTTCGCCGCTTCGAGATCCATGATGTTGGGTTCGATCGGCAAAtttctc                                                                                                     >  1:150929/1‑225 (MQ=255)                                                                                                                          aaacgccgAACGCCGGCGGCGGGCCCCTGGTCAGGGATGCGGCGGAACAGACCAATTCCCCCA‑GGGCGGCTGGCGTGAGTGCCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCt                                                                                              <  2:119192/227‑1 (MQ=255)                                                                                                                          aTACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACAAATATCCCCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCt                                                                                              <  1:451413/229‑1 (MQ=255)                                                                                                                                cgaACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCCCTTCGAGATCCATGATGTTGGTTTCGATCGGCCAATCGCGCCGTATCtgtgc                                                                                          >  1:732131/1‑226 (MQ=255)                                                                                                                                   aCGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCCTCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCCTGATGTTGGTTTCGATCGGCCAATTGCGCCGAATCTGTGTGtttc                                                                                     >  2:221338/1‑227 (MQ=255)                                                                                                                                     tcgtcgagcagTCCCGGATGCAGAGATGAGGCGGAACACA‑CACGATCAGCACGCGCGCGTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTGCCCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCAcc                                                                                   <  2:18612/223‑1 (MQ=255)                                                                                                                                     gcctggaagctgtaccGATTCAGGGAGGATGGGGAACAGACCAATATCCCCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCAcc                                                                                   <  1:506798/215‑1 (MQ=255)                                                                                                                                     ccctgcacggttccccGATCCAGGGATGAGCCGGAACAGACCAATATCGCCA‑GTGCGCTGGGCGTGAGTTCCGCCACCCAACCCGGTAGGGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATCCTGCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCAcc                                                                                   <  2:115717/216‑1 (MQ=255)                                                                                                                                     cccggcagcaGGCCCCGATTCAGAGAGGAGGCGGAACAGACCAATACCACCA‑GTGCGGCGGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCAcc                                                                                   <  1:280889/225‑1 (MQ=255)                                                                                                                                          cagcagTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCGCACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGCTGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCAcacggtt                                                                              >  2:318928/1‑223 (MQ=255)                                                                                                                                                     gATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCCCCAGGCCTTCGCCCGCAc                                                                   >  1:672696/1‑229 (MQ=255)                                                                                                                                                     gATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCACTCATCATACTTCTCGCCGAACAGCGCCATCACACCTTTCCCCTTCGCCGCCTCGGACTCCATGATTTTGGTTTCCACCGGCCAATTGCGCCGAACCTGTGTGTTCAACAGGACTTCGAACGcca                                                                   >  2:229833/1‑227 (MQ=255)                                                                                                                                                          agagTTGGTGCGGAACAGACCAATACCGCCA‑GTGCGGCTGGGGTGAGTGCCGCCCCACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAAt                                                               <  2:156950/228‑1 (MQ=255)                                                                                                                                                          agagATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCgag                                                                                                                                       >  1:43552/1‑156 (MQ=255)                                                                                                                                                            agaTGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGGGTTCACCAGGTCTTCGACCGCACGCAtctc                                                            >  1:702357/1‑229 (MQ=255)                                                                                                                                                                gatgCGAAACAGCCCAAGACCCCCA‑GGGGGGCTGGCGTGAGTACCGCCACCCAACTCGGTAGAGAAATCGCCCATGCCCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCtcttct                                                        <  1:712583/229‑1 (MQ=255)                                                                                                                                                                 atgCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCCCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGGTGTTGGTTTCGATCGGCAAATTGCGCCGAACCAGTGGTTTAACCAGGTTTTGTACCGCACGCATCtcttc                                                         >  2:304240/1‑227 (MQ=255)                                                                                                                                                                      gAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTc                                                  >  1:367211/1‑229 (MQ=255)                                                                                                                                                                            aCCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCCCGAATCTCTTCTGGTTTCATCGCt                                            >  1:504700/1‑229 (MQ=255)                                                                                                                                                                            aCCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTGGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCCCGAACTCCTTTGGGTTTAATCGCt                                            >  2:431424/1‑229 (MQ=255)                                                                                                                                                                                 tatCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCTTGATGTTGGTTTCCATCGGCAAATTGTGACGAATCTATGTGTTCAcc                                                                                   >  2:494005/1‑185 (MQ=255)                                                                                                                                                                                 tatCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGCTCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCCCTTCGtt                                       >  1:473097/1‑229 (MQ=255)                                                                                                                                                                                 tatCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAACTTGCGACGCATCTGTGTGTTCACCAGGTCTTCGACCGCACGCATCTCTACTGGTTTCTTCGCTTCCtt                                       >  1:700094/1‑229 (MQ=255)                                                                                                                                                                                 tatCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCCCGCATCTCTTCTGGTTTCATCGCTTCGt                                        >  1:725871/1‑228 (MQ=255)                                                                                                                                                                                 tatCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGt                                        >  1:446889/1‑228 (MQ=255)                                                                                                                                                                                      ccA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGCGATCCATGATGTTGGTTTCGATCGGCCAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTtttgtg                                  >  1:173866/1‑229 (MQ=255)                                                                                                                                                                                      ccA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGCCGAATCTTGGTGTTCACAAGGCTTTTGACCGCACGAATTCCTTCTGGTTTCATCGTTTTtgtgtgtg                                  >  2:250651/1‑229 (MQ=255)                                                                                                                                                                                          gTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTAGAGATCCATGATGTTGGTTTCGATCGGCAAATTCCGACGAATCTTGGTGTTCACCAGGGCTTCGACCGCACGAATCCCTTCTGGTTTCAACGCttgcgtgggggcaa                               >  2:392951/1‑217 (MQ=255)                                                                                                                                                                                             cGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAg                             >  1:399129/1‑228 (MQ=255)                                                                                                                                                                                             cGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGt                            >  1:31509/1‑229 (MQ=255)                                                                                                                                                                                               gacggtctgggtcccGCCACACAAATCGGTAGAAAACACGCCCATGCCTAGCACGCGTTCGCGATCATCCGTCTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCg                          <  2:269512/216‑1 (MQ=255)                                                                                                                                                                                               gCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCAAGCGTACGCGCTCATCATACTTATCGCCGAAAAGCGCCATCGCAACTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTATTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTACGTTGTGTgagactga                           >  1:269512/1‑224 (MQ=255)                                                                                                                                                                                                       gAGTCCCCCCACACAACTCGGGAGGGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCgca                   <  1:440749/228‑1 (MQ=255)                                                                                                                                                                                                            cccccACACAACCCGGTAGAGAAATCCCCCATGCTCAGCACGCGTACGCGCTCATCATTCTTCTTGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCAcc              <  1:44882/225‑1 (MQ=255)                                                                                                                                                                                                            ccGCCACACAACCCGGTAGAGAAACCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCAcc              <  1:216439/228‑1 (MQ=255)                                                                                                                                                                                                               ccACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTg           <  1:250651/228‑1 (MQ=255)                                                                                                                                                                                                                   ccaACCCGGTAGATAAACGCCCCATGCCCACGACGCTTACCCGCTCATCATTCGTCTCGCCAAACAGCGCCATCGCTCCTTTCGCTTTGGCCGCTTCGAGTCCCGTGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGtt      <  2:180712/228‑1 (MQ=255)                                                                                                                                                                                                                     aaCTCGGTAGACAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTa     <  1:221338/228‑1 (MQ=255)                                                                                                                                                                                                                       cTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCCCGAATCTCTTTTGGTTTCATCGCTTCGTTGTGTGTGAAGTCGAAGCGCAGAACCTTGTCGTTAAcc  >  1:655919/1‑229 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                    TACTGAGCGCACTTTATCAGCCAGATTATTGCTATCGCCTTTCAGCAGATGCGCGACTTCGCTTAAGCGATCGCTGTCTGCATGAACGGTGGCGATAGCACCTTCTCCGGTTACCGCTTCGATACGACGAACGCCTGCAGCAGTACCCGATTCAGAGATGATGCGGAACAGACCAATATCACCA‑GTGCGGCTGGCGTGAGTACCGCCACACAACTCGGTAGAGAAATCGCCCATGCTCAGCACGCGTACGCGCTCATCATACTTCTCGCCGAACAGCGCCATCGCACCTTTCGCTTTCGCCGCTTCGAGATCCATGATGTTGGTTTCGATCGGCAAATTGCGACGAATCTGTGTGTTCACCAGGTCTTCGACCGCACGAATCTCTTCTGGTTTCATCGCTTCGTTGTGTGAGAAGTCGAAGCGCAGCACCTTGTCGTTAACC  >  NC_000913/2819805‑2820246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑