| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,909,227 | A→C | 33.0% | G146G (GGT→GGG) | pyrG ← | CTP synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,909,227 | 0 | A | C | 33.0% | 32.7 / 4.0 | 34 | G146G (GGT→GGG) | pyrG | CTP synthetase |
| Reads supporting (aligned to +/- strand): ref base A (10/12); new base C (5/7); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGG > NC_000913/2909000‑2909455 | gCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCa > 1:548225/1‑228 (MQ=255) caggtgcgttcgCCGGAACAGCGGGATGTGAACGAAAAAGCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGGGTGGTGAGTCGGTTTGGTTTTGACTGACCCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCAcc < 2:273681/223‑1 (MQ=255) cggttggtcgggcGGAAAAGCGCGTCCTGAACTATAATGCAGGATAT‑AAGGCTGGATACCGAAGGAGAGCAGCTCTTTTACAGAGTGGTAACTCGGTTTGGTTTTCACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGTATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCAcc < 2:431964/216‑1 (MQ=255) cggcggaatgggccgATGTGAACGAAAAGTCAGGATGG‑GCGGCTGGATGCCGATGGGGAGAAGCTCTTGTACCGAGTGCTGAGTCGGTTTGGTTTTGACCTCACCAGACGCTGCCATGTACGGCCCCGGCGTCAGGTGCATAAACAGTGTGGTCTCAGGCCCAATTTCAAAAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTCccg < 2:750512/216‑1 (MQ=255) gAACAGCGCGATCTGAACGACAAATCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGATTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAACCAGAGTGGGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCCCTTCGAGGCAAGGCAAGGATTCGAAATCCCCTACTGTActcgcatt > 2:249159/1‑222 (MQ=255) accAGCGCGATCTGAACGACAAACCAAGATGT‑CAGGCTTGATCACTATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGTTTGGTTTGAACTTCCCCAGCCGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGGGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGAAAGGATTCGATATCCCCTACTGTACCGCCGAtt < 1:683323/227‑1 (MQ=255) tgctgtatGAACGATAAATCGGGATGT‑GAGGCTGGAGACCGATGGAGACCAGCTCTTTTACAGAGTGCTGAGTCGGTGTGGTTTTGACCTCCCCAGACGCTGCCATGGACGGCCCTCGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTCGCAAATCTCTTCAAGGAACGGCAAGGATTCTATATCCCCTACTGTACCGCCGATTTCTAc < 2:250850/222‑1 (MQ=255) gtggatcgaaaCGACAAAGCAGGGTGT‑CAGGCTGGAACCCGATGGAGGGCAGCCTTTTTACAGAGTTCTGAGTGGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGTGGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTCCTGTACCGCCGATTTCTAc < 1:308604/220‑1 (MQ=255) ggttgtCTTAACGACAAATCA‑GAGGTACGGGTTGGATACCGTTGGGGGGCAGCCTTTTTACAGAGTGCTGAGGCGGTTTGGTTTTGCTTACACCAGCCGTGGCCATTGACGGCACCAGCGTCAGGTGCTAAAACAGGGTGTGCTCACGGCCAATTTCAACAGCCATCGGGCGAATCGCTTCGAGGAACGGCAAGAATTCGATATCACCTACTGTACCGCCGATTTCTAc < 2:638557/224‑1 (MQ=255) ggcgATCTGAACGACAAATCCGGATGG‑CAGGCTGGATTCCGATGGAGAGCAGCTCTTTTACAGAGTGCTGGGGCGGTTTGGTTTGGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTAc < 1:733796/228‑1 (MQ=255) cgcgATCGGAACGAAAAATCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTAc < 1:564212/229‑1 (MQ=255) gAACGACAAATCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCCGGTGCATAAACAGAGTGGGCTCACGGCCAATTTCAAAAGCCATCTGGCGAATCGCCTCGGGGAACGGCCAGGATTCGAATTACCCCACTTTACCGCCGCtttttccccatac > 2:184990/1‑217 (MQ=255) cAAATCAGGATTG‑CAGGCTGGTACCCGATGGAGAGCCGCTTTTTTACAGAGTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCCCCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCCCCTACTGTACCGCCGATTTCTACCAGTACTACGTCa < 1:229881/228‑1 (MQ=255) aCCGATGGAGAGCAGTCTTTTTCCAGATTGCTTAGTCGGTTTGGTTTTGCCTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACGGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCTCTTCGAGGAACGGCAAGGATTCGATATCCCCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAcg < 1:2303/229‑1 (MQ=255) tttgtaaaggggacagctgtgaCAGAGTGCTGGATCGGTTTGGTTTTGACTTCCCCAGACGCTGCCATGTACGGCACCAGCGGAAGGTTCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAcg < 1:314386/207‑1 (MQ=255) agcagcTCTTTTACAGAGTTCTGGTTCGGTTTGTTTTGGACTTCACCAGACGCTGCCATTTACGCAACCAGCGTCAGTTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAAGCGCTTCGAGGAACGGCAAGGATTCGATATCCCCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGa < 1:581675/229‑1 (MQ=255) agTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGGGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCCCCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCACCACGCGCTCTTTGATTGCGCTAGTGCtgt > 1:640213/1‑228 (MQ=255) tGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCCCCTACTGTAACGCCGATTTCTACCCGTACTACGGCATGACCTTCGCCCCCTTCCCGCACCCGCTCTTTGtttcgactcttataaaca > 2:314386/1‑213 (MQ=255) tGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTCAGTCATGACCTTCCCCCCCTTCCAGCCCCGCCTCTTTCATTGCGTTAGGAAAGTGCg > 2:425672/1‑229 (MQ=255) gtttggtttTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCCCCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAcg < 1:364850/191‑1 (MQ=255) gtttggtttTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCAcg > 2:364850/1‑191 (MQ=255) gtttggtttTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGAAATCCCCTACTGGACCGCCGCTTTCTACCAGTACTAAGTCATGACCTTCCCCACCTTCCAGCCGGCGCTCTTTGATTGCGTTAgagtgtttcgcaaaaacctc > 2:198966/1‑210 (MQ=255) gtttTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCAATCTGGCGAATCGCTTCGAGGAACGGCAAGGCTTCGATATCACCTACTGTACCGCCGATTTCTACCAGTCCTACGTCATGAACTTCCCCCCCTTCCCGCACGCGCTCTTTTATTTCGTTTGTCATGTTCGGAAACAACTGCaacg > 2:487911/1‑226 (MQ=255) cACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTCCTACGCCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTCCGTTATTGATGTGCGGAATAACCTGCACGGTTCGCCCCAGGCAGTCACCGCGGCGTTCttt > 2:231018/1‑228 (MQ=255) cACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTCGGGATGTGCGGAATAACCTGCACGGTTGCGCCGTGGTAGTCACCGCGGCGGTCgtt > 1:588823/1‑228 (MQ=255) cACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCttt > 1:161828/1‑228 (MQ=255) cACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACAACGTCATGACCTTCGCCACCTTCCACCCCGCGCTCTTTGATTCCGTTAGGGACTGGCGGACTACCCTCCACGCTGCCGCCCTGGTAGTTCACGCGGCGGTAttt > 1:354108/1‑228 (MQ=255) gcgcCAGGGCCATAAACAGAGTGGGCTCCCGGCCAATTTCAACAGCCATCGGGCGAATCGCTTCGAGAAACGGCAAGGATTCGATATCACCTACTGTCCCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAg < 1:700517/229‑1 (MQ=255) gCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGGGGCGTTCTTTACGCAg > 1:577277/1‑229 (MQ=255) gggTGGAAAAAAAGAGTGTGCCACCCGCCCATTTCACCCTCCTTGTGGTGAATGCTTTAGGTGAACGGCCTGGGTTCGTTCTCACCTACGTTACCGCCGATTTCTACCAGTTCTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTATTTACGCAGAACGt < 2:640212/228‑1 (MQ=255) ggTGCAAAAAAAGAGTGTGCCCTCGGCCAATTCCAACAGCCATCTGGCGAACCGCTTCGAGGAACGGCAAGGATTCGATATCACCTCCTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTc < 1:249159/229‑1 (MQ=255) gTGCATAAACAGATTGTGCCCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTCCTGTTCCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTc < 1:585380/228‑1 (MQ=255) gagTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGCATTCGATATCACCGACTGTAACGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGCGATGTGCGGACTAACCTGCACGGTTGGGCCGCGGTAGACACCGCCGCGTTCGTTACGCAAACAGTCAGGGTAGATAca > 2:636658/1‑228 (MQ=255) agTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCCCGCGCTCTTTGATTGCGTTAGTGATGTGGGGAATAACCTGCACGGTTGCGCCGGGGTAGTCACCGCGGCGTTCTTTACGCAAAACGCCAGGGTAGATAcc > 2:585379/1‑227 (MQ=255) cGAGGAACGGCAAGGATTCGATATCCCCTACTGTTCCGCCGATTTCTGACAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGa < 1:363388/228‑1 (MQ=255) aCCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCTGGGTGAAGTCGTTGCGGCGGCGCCATTTGGTCAGAAGGGAACGCTCGTGGTGCCcccgggaccgc > 1:689371/1‑223 (MQ=255) aCCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCAATTTGGGACCAAAGAAACGCGCGAAGTGGCccatgtcccg > 1:444381/1‑226 (MQ=255) | GCACGTTCGTTCGCCGGAACAGCGCGATCTGAACGACAAATCAGGATGT‑CAGGCTGGATACCGATGGAGAGCAGCTCTTTTACAGAGTGCTGAGTCGGTTTGGTTTTGACTTCACCAGACGCTGCCATGTACGGCACCAGCGTCAGGTGCATAAACAGAGTGTGCTCACGGCCAATTTCAACAGCCATCTGGCGAATCGCTTCGAGGAACGGCAAGGATTCGATATCACCTACTGTACCGCCGATTTCTACCAGTACTACGTCATGACCTTCGCCACCTTCCAGCACGCGCTCTTTGATTGCGTTAGTGATGTGCGGAATAACCTGCACGGTTGCGCCGAGGTAGTCACCGCGGCGTTCTTTACGCAGAACGTCAGAGTAGATACGACCCGTGGTGAAGTTGTTGCGGCGGCTCATTTTGGTACGAATGAAACGCTCGTAGTGCCCCAGGTCCAGG > NC_000913/2909000‑2909455 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |