Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,436 G→A 24.3% intergenic (‑182/‑25) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4360GA24.3% 65.7 / 5.2 44intergenic (‑182/‑25)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (2/27);  new base A (2/8);  total (9/35)
Fisher's exact test for biased strand distribution p-value = 2.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGT‑TGACAG‑AGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATT  >  NC_000913/3206219‑3206652
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                               
cTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GCGGGG‑G‑AAT‑TTTTC‑C‑A‑ACAAACAa‑aaa                                                                                                                                                                                                                     >  2:437884/1‑229 (MQ=255)
        cTTTGGCTAAAGGCCAGGTATTTACCATCGTTAATTTTCCGCAGGTGTGATAAGCGCTGTGTATTTCGCAAAACATTTCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCGCGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAg                                                                                                                                                                                                            <  2:741473/229‑1 (MQ=255)
        cTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAg                                                                                                                                                                                                            <  1:486105/229‑1 (MQ=255)
        cTTTGGCCAAAGGCCAGCTATTCAGCCTCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCCC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGa                                                                                                                                                                                                             <  1:108343/228‑1 (MQ=255)
           tGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTT‑C‑AACCAAAAACAAAAAA‑ATGGAGt‑t                                                                                                                                                                                                         >  1:599803/1‑229 (MQ=255)
           tGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCACGTTGATTCAC‑GACCTGTTAATTACATATTAACGGTGGCAAAACATTTAAAACAAC‑ATTTTTA‑GTGGTAGTTT‑TGG‑GC‑GGT‑G‑GTA‑TTTTT‑T‑ATACAaaaaa‑aaaaaaatgaggta                                                                                                                                                                                                          >  2:181515/1‑220 (MQ=255)
                  aGGCCAGTTTTTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCCCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCa‑tg                                                                                                                                                                                                  <  2:50449/227‑1 (MQ=255)
                        gtttttctggttCGTTAATTCTCCTCAGTTGTGATAAGCGCAGTTTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑a                                                                                                                                                                                         <  2:21447/218‑1 (MQ=255)
                             tCAGCTTTGTTAATTAGCCGTCGTTGTCATAAGTGCAGTGTATTTCGCAAACCATTTCACTTCCCGGCTCTGGCGCAATACTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGGAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AACAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGta                                                                                                                                                                                    <  2:534453/229‑1 (MQ=255)
                              cAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GAG‑GC‑GGG‑G‑AAT‑ATTTT‑C‑ATACACATAA‑AAAAA‑ATGAAGA‑Tgtgaggtatggagaaaagaa                                                                                                                                                                                     >  2:740454/1‑210 (MQ=255)
                               aGCATCGTTAATTTACCGCCGTTGTGATAAGCGCAGTTTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGta                                                                                                                                                                                    <  1:181515/227‑1 (MQ=255)
                                gCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGtaat                                                                                                                                                                                  <  1:411251/228‑1 (MQ=255)
                                   tCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTT‑T‑TGACCAATAA‑AAAAA‑ATGGCGG‑TGCCA‑CGATTGGCG‑ACAGtaattatg                                                                                                                                                                              >  1:21928/1‑225 (MQ=255)
                                     ggtAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCaa                                                                                                                                                                            <  1:416389/227‑1 (MQ=255)
                                     gTTAATTAACCGCAGTTGTGATAAGCTCAGTGTATTTCGCAAAACATTTCCATTCCCTGCTCTGGCGCAATATTCAATCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CGGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCaa                                                                                                                                                                            <  1:238480/229‑1 (MQ=255)
                                      ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAACAATATAAAACCAC‑ATTTTTA‑TTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTT‑T‑TTGCACACAC‑AAAAA‑CCAGAAT‑TGCCA‑GGCTTCGCG‑AAAGAACAAAGCaaa                                                                                                                                                                           >  1:215250/1‑228 (MQ=255)
                                      ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGG‑G‑AAA‑TTTTT‑C‑AGAAAAAATA‑AAAAA‑CTAGAGTGTGTC‑‑TGTTGAGCG‑AGAAGAATAAGAAAc                                                                                                                                                                           >  1:485848/1‑229 (MQ=255)
                                         aTTAACCGGAGTTTTGAATAGCGCAGTGTAATTCGCAAAACCTTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATGTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAgg                                                                                                                                                                        <  1:495152/229‑1 (MQ=255)
                                          ttATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGc                                                                                                                                                                       <  2:527468/229‑1 (MQ=255)
                                               cGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑a                                                                                                                                                                                         <  1:664120/206‑1 (MQ=255)
                                                 cAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGaa                                                                                                                                                                 <  1:473486/228‑1 (MQ=255)
                                                                 aGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTACTTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGTAG‑AAT‑TTTTC‑T‑ATA‑AAATAA‑ACAAA‑CTGGAGT‑TGTCA‑TCATGAGCG‑AAGGAGATAAGAAAACGGCaaaggagaagatgtgacagatag                                                                                                                                                >  1:42863/1‑207 (MQ=255)
                                                                     tATTTCGCAAAACATTGCCAGTTCCTGCTTTGGCACAATATTCATTCTTATTTTCGCTTTAATTCAC‑GAACTGTTAAATACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGT‑TGACAG‑AGATTGTCGc                                                                                                                                          <  2:215250/229‑1 (MQ=255)
                                                                      aTTTCCAAAAACTTTGCCTTTCCCTGCTCTGGTGAAATATTCATTCTTATTTCCGTGTTTATTCAC‑AACCTGTGAATTAAATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑GTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAACCACCAGGCATTGAATAAGT‑TGACAG‑AGATTGTCGca                                                                                                                                         <  2:389790/229‑2 (MQ=255)
                                                                                                               cattcTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAgg                                                                                                                                                                        <  1:541815/159‑1 (MQ=255)
                                                                                                                      ttgttaggaggttatacagtaaCTTTTATTTACATTGTGGCGGTGACAAAAAATATAAAACCAT‑ATTTTGA‑GTGTTAGTGT‑GTG‑GG‑GGT‑A‑ATT‑TTTAC‑C‑AGAAAAATAA‑AAAAC‑CGGGAGT‑GGCCA‑TGAGGAGCA‑AAAGTAATAGGCAACAGGTATTAAAAAAGT‑GGAGAG‑AGATGGTCGATATTTTCACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTTGa                                                                                          <  2:550467/209‑1 (MQ=255)
                                                                                                                              tttGTTTCACGGAATTTTTAATTACATATTAGCGGTGGCAAAAAATAAAAAACCAC‑ATTTTTA‑GGGGTAGTTG‑GGG‑GG‑GGA‑G‑AAT‑TTTTC‑A‑AAAAAAATAA‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AATGTAAAAAGCAACAGGAGTTGAATAAGG‑GAACAG‑AGATTGTCGTAATTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa                                                                                  <  2:389136/228‑1 (MQ=255)
                                                                                                                               tttaattccagacttttaaagtACATTTTCGGGGTGACAAAAAATAAAAAACAAG‑ATTTTGA‑GGGGGAGTTG‑GTG‑GC‑GGT‑A‑AAT‑TTTCC‑C‑AGAAAAAAAC‑AAAAA‑GGGGGGT‑GGTCA‑AGATAAGCA‑AAATTAAAAAGCAACAGGAGGTGAATAAGT‑TGAAGG‑AGATGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa                                                                                 <  2:99679/208‑1 (MQ=255)
                                                                                                                               ttGATTCAC‑GAACTGTTAATTCTATTTCCACGGTGGCAAAAAAAATAAAACAAC‑ATCTTGA‑TGGGTAGTTG‑TCG‑GC‑GTG‑A‑AAT‑TTTGC‑A‑AGACAAATAC‑AAAAA‑CTAGAGT‑AGCTA‑TGATGAAGG‑AAAGTAAAAAGCAACGGTAAGGTAATAATT‑TGACAC‑AGATTGTCGTCAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa                                                                                <  1:427707/229‑1 (MQ=255)
                                                                                                                                              gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTT‑C‑AAACAAAAAC‑AAAAA‑CAGTAGT‑TGGCA‑TGATGAGCG‑AAAGGAAAAAACAAAAAGCAGGGAAAAAAA‑TGTCAG‑AGAATGTGGCTAAAATTATCACCATGATTTTTACCCCAAAGCCCGATGACGTGGGGGGTAAACTAAAACACCTCAAGGaag                                                                   >  1:120624/1‑226 (MQ=255)
                                                                                                                                                     atttttgcatgtgatcAAAAAATATAAAACCAC‑TTGTTAA‑GTGTTGGTTT‑GGG‑GA‑TGT‑A‑AAT‑GTTCC‑A‑AGAAAAATAA‑AAAAA‑CTGGAGG‑TGCTA‑TGTGGAGAG‑AAAATAATAGGCAACAGGCAGTTAAGAGTT‑TAACAT‑AGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAAcg                                                           <  2:42863/214‑1 (MQ=255)
                                                                                                                                                          tgcaggggtgaaaaaaaaaaaaaACCAA‑TTTTTTA‑GGGGTTGGTT‑GGG‑GC‑GG‑‑G‑AATGTTTCC‑C‑AAAAAAATAC‑AAAAC‑CGGGAGT‑GGCCA‑GGATGAGCG‑AAATTAAAAAACAACAGGCAGAGAAAAAGT‑TGACGG‑AGATTGACGTAACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACgtcgtc                                                      <  1:108853/218‑1 (MQ=255)
                                                                                                                                                                 tGGAAAAAAATAAAAAACCAC‑ATTTTTA‑GTGGGAGTTT‑GGG‑GC‑GGT‑A‑AAT‑TTTCC‑A‑AGAAAAATAC‑AAAAA‑CGGGTGT‑TGCCA‑TGATAAGCG‑AAAGTAATAAGAAACGGGCAGTGAATAAGT‑TGACAG‑AGATTGCCGCTACCTTTACCGCCATGATTTCTACCCGAAt                                                                                                            <  1:553293/168‑1 (MQ=255)
                                                                                                                                                                  ggaaaaaaaTATAAAACCAC‑ATTTTAA‑GTGGTAGTTT‑GGG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AAAAAAATAC‑AAAAA‑CTGGATT‑TGCCA‑TGATAGGCG‑AAATTAATAAGCAACAGGCAGTAAATAAGT‑TGAAAG‑AGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                              <  1:115981/226‑1 (MQ=255)
                                                                                                                                                                  ggaaaaaaaTAAAGAACTCG‑ATTGTTA‑GT‑GTGGTTTGGTG‑GT‑GGT‑‑‑AAT‑TTTTC‑CAAGAAAAAT‑C‑AAAAA‑ATGGGAG‑TGCCAGTAATGATCA‑AAAGTAAAAAGAAACAGGATGTTAATAAGT‑TGAAGG‑ATATTGTCGATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                              <  1:756254/226‑1 (MQ=255)
                                                                                                                                                                  gcaaaaaaaTATAAACCCTA‑ATTTTTA‑GTGGTAGTTT‑G‑G‑GG‑GGT‑GAAAT‑TTTTC‑A‑AGACAAATAA‑AAAAA‑CTGGAGT‑TGCAA‑TGATGCGCA‑AAATTAAAAAACAACGGCCAGTTAATAAGT‑AGACAG‑AGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                              <  1:2100/226‑1 (MQ=255)
                                                                                                                                                                    gaaaaaaaaTAAAAACACAATTTTTAGGGGGTAGTTT‑GGG‑GG‑GGA‑A‑AAT‑TTTAC‑C‑AAAAAAAAAA‑AAAAA‑CGGGGGT‑TGCAT‑AGGGTAGCA‑AAATAAATACACAAGAGGTAATTAATAAGT‑TAAAAG‑AGGTTCGCGTAAATTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                              <  2:21928/228‑1 (MQ=255)
                                                                                                                                                                       aaaaaaTAAAACAAA‑TTTTTTT‑GTGGTAGTTG‑GTGCGC‑GGT‑‑‑AAT‑TTGGC‑C‑AGA‑AAAAAC‑AAAAAGTTGAAGT‑GACAA‑TGAGGAGCA‑CAATAAACAAGAACGGGGCATTGAATAAGT‑AGAAGG‑ATTTTGGCGCAAAATCTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAACCGTCGTCGATGGGGAAAAtt                                         <  2:660610/229‑1 (MQ=255)
                                                                                                                                                                         ttttaaaaaCCAT‑TTTTTTT‑GGGGGTGTTT‑GGG‑GG‑GGT‑‑‑AAT‑TTTTCAC‑AACAAAAAAA‑AAAAA‑GTGGGGT‑TGCAA‑TGATGAGCG‑AAAGAAAAAACCAACAGAC‑GGGAAAAAGTGTGACAG‑AGATTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                       <  2:292781/224‑1 (MQ=255)
                                                                                                                                                                         atttaaaaaCCAT‑TTTTTAG‑GTGGTAGTTT‑GGG‑GG‑GGT‑‑‑AAT‑TTTTC‑CAAAAAAAATAA‑AAAAA‑CGGAAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGAAACAGGAGTTGAATAAGT‑TGAAAG‑AGTTTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTAt                                       <  2:120624/224‑1 (MQ=255)
                                                                                                                                                                                                tggtggTTT‑GGG‑GC‑GAT‑A‑AAT‑TTTTC‑C‑AGGAAAATAA‑AAAAC‑GGGGATT‑TGCCA‑TGATGGGAG‑AAAGTAATAAGCAACAGGTAGAGAATAAGT‑TGAAGG‑GTCTTGTATATAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                  <  1:76812/229‑1 (MQ=255)
                                                                                                                                                                                                tGGTATTTT‑GGG‑GG‑GGT‑A‑AAT‑TTGTC‑A‑AAAAAAAAAA‑AAAAC‑GTGGATG‑TGCAA‑GGATG‑GCGAAAAGAAATAACCAACGGGAGGGAAATAAGT‑GAAAGG‑TGATGGCCGCTAATATAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGacaa                  <  2:280835/229‑1 (MQ=255)
                                                                                                                                                                                                                   t‑G‑ATT‑TTTCC‑C‑AAAAAAATAA‑AAAAA‑CGGGATT‑TGCCA‑TGATGAGAA‑AAAGTAATAAGCAACGGGCAGTGAATAAGT‑TGAAAG‑AGTTTGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAtt  <  1:362402/229‑1 (MQ=255)
                                                                                                                                                                                                                     g‑AAT‑TTTAC‑C‑AGACAAATAC‑AAACG‑GTGGATT‑TGCCA‑GGTGTGGCG‑AAGTTAAAAGGCAACAGGAAGTGAATAAGT‑T‑AGAGAAGTTGGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGAtt  <  1:484934/228‑1 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                               
CTGCAAGTCTTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAC‑GAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCAC‑ATTTTTA‑GTGGTAGTTT‑GTG‑GC‑GGT‑G‑AAT‑TTTTC‑C‑AGACAAATAC‑AAAAA‑CTGGAGT‑TGCCA‑TGATGAGCG‑AAAGTAATAAGCAACAGGCAGTGAATAAGT‑TGACAG‑AGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAAAGCGATT  >  NC_000913/3206219‑3206652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑