Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,277,454	T→A	10.6%	Y40* (TAT→TAA)	yhaV →	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,277,454	0	T	A	10.6%	165.3 / 2.3	81	Y40* (TAT→TAA)	yhaV	toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (30/38);  new base A (2/7);  total (32/49)
Fisher's exact test for biased strand distribution p-value = 2.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGA  >  NC_000913/3277226‑3277665                                                                                                                                                                                                                                               |                                                                                                                                                                                                                          atatCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAATTCGAGACATTAAAGGGAAA‑AAATCCGGAAaataat                                                                                                                                                                                                                           >  2:227779/1‑229 (MQ=255)  tttCCAGAAAAACCCGAAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATc                                                                                                                                                                                                                          <  2:624579/227‑1 (MQ=255)  tgtccagacacaccagtaaaaaaCTCGTCCATTCAACATTCAAAAAGGAAAGAAACTTGTTGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATc                                                                                                                                                                                                                          <  1:500266/212‑1 (MQ=255)         aacaacCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTTATGATGAAATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGTTT‑AA‑TGGTTGGGCGC‑TATA‑TACTCATCCCCGTTTT‑C‑AGGAAACCTACGACC‑CTTTA‑TCTGCCGAATTCGAGACATTAAAGGGAAA‑AAAACCTAAAAAATAACAaaaaaa                                                                                                                                                                                                                   >  2:350867/1‑229 (MQ=255)         aacaacCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGTAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGGGACATTAAAGGGAAA‑AGATCCTGAAAATTATAAGAaaaa                                                                                                                                                                                                                   >  2:728125/1‑229 (MQ=255)         aacaacCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGACACCTACGACG‑CTTTA‑GTGGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCATGAAAATTATCaggcaaa                                                                                                                                                                                                                   >  1:47639/1‑224 (MQ=255)         aacaacCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGCTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGGAAATTATCAG‑Gaaaa                                                                                                                                                                                                                  >  1:62319/1‑229 (MQ=255)           caacCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGGT‑AA‑TGGTTGGGGGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAGATCGAGACATTAAAGGGAAA‑AAATCCTGAAAATTATAAAAGaaaaa                                                                                                                                                                                                                 >  2:725051/1‑228 (MQ=255)              cccGCACTAAACTGGTGCATTCAACGTTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGATATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGGCGAAGGCGGGAAATTAAAGGTGTA‑AGATCCAGAAAATCATCAGCAAAAAgcac                                                                                                                                                                                                              >  2:466420/1‑227 (MQ=255)              cccGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGCGACATTAAAGGGAAA‑AGATCCTGAAAATTATCCGAGAAAAgcgg                                                                                                                                                                                                              >  1:298962/1‑227 (MQ=255)              cccGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGaaaaacgg                                                                                                                                                                                                              >  1:405859/1‑225 (MQ=255)              cccGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACATACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGCTCCTGAAAATTATCAGAGAAAAgccg                                                                                                                                                                                                              >  1:740379/1‑229 (MQ=255)              cccGCAAAAAACTCGTCCATTAAACATTCAACAAGGCAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGGGCCGAAGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAACCCTACGATG‑CTTTA‑GTAGCCGCAGCCGAGACGTCAAAGGGAAC‑AATTCCGGAAATATATCAGATCAAAgacc                                                                                                                                                                                                              >  2:445417/1‑226 (MQ=255)              cccGAAAAAAACTGGTCCTTTTAACTTGAAAAAAGGAAAAAAACTTGTCGCAGGCTTGGACGTCAACATGGATGA‑GGGATTGGGCGACGACGAATAAGGGATTTTCCACAAAGGGTT‑AA‑GGGTTGGGCGT‑TATA‑CGCTCATCCTTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTGGCCAAAGTCGAGACATTAAAGGGAAA‑AGATCCTAAAAATTATCAGAGAAAAgccg                                                                                                                                                                                                              <  2:163477/229‑1 (MQ=255)                  cAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCca                                                                                                                                                                                                           >  1:644764/1‑228 (MQ=255)                   aaaaagCTCTGCCATTAAACATTCAAGAAGGAAAGAAATTTGCCGCGGGCATGGACGTAAAAATGGAGGATGAGATG‑GGCGCGGACGAATAAGGGATTTTCCACAAGGGGTT‑AT‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTC‑A‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGAAAA‑AGATCTTGAAAATTATCAGAAAAAAGCCGCcaca                                                                                                                                                                                                         <  1:218036/229‑1 (MQ=255)                   aaaaacCTCGCCCATAAAACATTAAACAAGAAAAGAAACTTGTCGCTGGCATGGAGGTCAAAATGGAGGATGGGTTG‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑GGGTTGGGCGC‑TATA‑TGCCCACCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATAAAAGGAAAA‑AGATCCTAAAAATTATAAGAGAAAAGCCGCcaca                                                                                                                                                                                                         <  2:644763/229‑1 (MQ=255)                   aaaaaaCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGCCG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAgcggcccc                                                                                                                                                                                                          >  1:116363/1‑226 (MQ=255)                   aaaaaaCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGCCG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCccca                                                                                                                                                                                                         >  1:576611/1‑229 (MQ=255)                   aaaaaaCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGGGAAAAGCCGCcaca                                                                                                                                                                                                         >  1:506308/1‑229 (MQ=255)                             tCCATTCAACCTTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGGGAGT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTAATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCTTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTgg                                                                                                                                                                                               <  1:752511/229‑1 (MQ=255)                             tCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAGTAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCCCAAAGGTATTgg                                                                                                                                                                                               >  2:521007/1‑229 (MQ=255)                                 ttcaacattcaacaAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGATTAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGCGACATTAAAGGGAAA‑AAATTCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTAATGgggga                                                                                                                                                                                           >  2:741620/1‑228 (MQ=255)                                 ttcaacattcaacaAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCgga                                                                                                                                                                                           >  1:540082/1‑228 (MQ=255)                                                ggAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGAT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGAGGGAGACCATaaaaggat                                                                                                                                                                            >  1:75508/1‑224 (MQ=255)                                                  aaagaacttggtgtggggaaTGGACGTAAACTTTGAGTTGGATATT‑GGCGCCGACTATTCATGGATTTCCCACAGAGGTTA‑AA‑GGGTTGGGCGT‑TATA‑TTCTCATCCCTTGTTG‑C‑AGGAAACCAACGACT‑CTTTA‑TTTGCCAAAGTCGATACATTAAAGGGAAA‑ATATCCTGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTg                                                                                                                                                                         <  1:741621/210‑1 (MQ=255)                                                    agaaaCTTTGCGCTGGCAGGGACGTAAACAGTGATGATGGGATG‑GGACACAGCGAATAAGGGATTTTCCACAAGGGGTT‑AA‑TGGTTGGGGGT‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATAATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTgag                                                                                                                                                                       <  2:412583/229‑1 (MQ=255)                                                     gaaaCTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTGGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCATAGCAAAACCGCCACAAAGTTATggggggaaatcagaaaaacagagtagg                                                                                                                                                                       >  2:651520/1‑208 (MQ=255)                                                     gaaaCTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACATACGACG‑CTTTC‑GTTGCCGAAGTGCCGCCATTACAGGGAAA‑AGATCCTGAAAATTAACAGCGAAAAGCCCCCACAAAGTAATTGGAGGGTGTcccctacaaggattggg                                                                                                                                                                       >  2:366521/1‑214 (MQ=255)                                                      aatcTTGTCGCTGGCAAGGATGTCAACATTGATGATGAGGTT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGA‑TATA‑ATCTCATCCTTGTTTA‑C‑AGGAAACCTACGACG‑TTTTA‑GTTGCCGAAGCAGAAACATTAAAGGAAAA‑AGATCCTGAAAATTAACAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTgagga                                                                                                                                                                     <  1:149928/226‑1 (MQ=255)                                                              cGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTCCGACG‑ATTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCa                                                                                                                                                             <  1:53544/229‑1 (MQ=255)                                                                        tgcGTAAACATGGATGAGGAGATG‑GACGACGACGAATAATGGCTTCTGCACAAGGGGTT‑AA‑TGGTTGGGCAC‑TTGA‑CGCTATTCCCGGTTTT‑A‑AGGAAACCTACGCAC‑ATTAA‑GTAAGCGAAGTCGATACATTAAAGGAAAA‑AGACCATGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATcc                                                                                                                                                   <  1:342149/227‑1 (MQ=255)                                                                                          gagaTT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCCTAAAGAA‑CATGAGGGGCAAATCACGGTCAATACAT‑CATCAACGGCATT‑CCg                                                                                                                               >  1:624599/1‑229 (MQ=255)                                                                                               t‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCAGCCACAAAGTTATTGGCGGTAGACAATAAAGGG‑ATTTAGGGGCATAACACGGGCAAACcacacataccgtcattccctcctg                                                                                                                            >  1:290456/1‑207 (MQ=255)                                                                                                   gcaccacaaaactttgtttttCCCAAAATGGTT‑AA‑GGGTTGTGCTC‑TAGA‑TCTACATCCCTTTTTT‑A‑TAAAACCCGACGACA‑TTTTA‑TTTCCAAAGGTCGAAACTTAAAAGGCAAA‑GGACCCTAAAAATTCTCTGAAAAATGCCGCCCCAAAGTTATTGGCGGTAGCCCATAAAGTG‑ATTTAGGAGCATATCGCGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGcg                                                                                                                       <  2:386779/212‑2 (MQ=255)                                                                                                   ctaggaagaATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGTGA‑TAAA‑TGCTCACCCTTGTTTG‑C‑AGGAAACCTACGCCG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCa                                                                                                                       <  2:160865/222‑1 (MQ=255)                                                                                                    tgagtctaataatGGGTTTCCCCAAAGGGGTT‑AA‑TGG‑TGGGCGCGTAAG‑CGCTCATCCCTGTTTC‑C‑AAGAACCCGACGACG‑ATTTA‑GTTGAAGAAGTCGAGAAATAAAGGGAAAA‑AGAGCCTGAAAAGTACAAGAGAAAAGCCGCCACAAAGGTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGAATT‑CCGTCATGGCa                                                                                                                       <  1:350867/221‑1 (MQ=255)                                                                                                    cggcgaccaattagggttttCCCCAAAAGGTTGAA‑TGGTTGCGCGT‑TATC‑GGGTCATCCCTCTTTC‑C‑AGGAAACTAACGGCG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTAACAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCa                                                                                                                       <  1:366521/213‑1 (MQ=255)                                                                                                         ccaataagGGATTTCCCAAAAGGGGTT‑AA‑TGGTTGGGCGC‑TTAA‑TGCTAACCCTTGTTTT‑C‑AGGAAACCTACGACG‑TTTAA‑GTACCCAAAGCCGAGAATTTAAAGGAAAA‑AGACCCTGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑Gt                                                                                                                <  1:202446/227‑1 (MQ=255)                                                                                                          aaataatGGATTTCCCACAAAGGGTT‑AA‑GGGTTGGGCGC‑TATA‑TGCTCATCCGTTTTTT‑C‑AGGAAACTTACGACG‑TTTTA‑GTTGCCGAAGCCGAGAAATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑Gtt                                                                                                               <  1:521007/228‑1 (MQ=255)                                                                                                                ttgATTTTCCAAAAAGGTTA‑AA‑GGGTTGGGCTC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTGCGACG‑TTTTA‑GTTGCCAAAGTCGAAAAATTAAAGGGAAA‑ATACCCTGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGct                                                                                                          <  1:647480/226‑1 (MQ=255)                                                                                                                   aTTTCCCAAAAGGGGTT‑AA‑GGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑A‑AGGAAACCTCCGACT‑CTTTA‑GTTGCCGAAGTCGAGACATAAAAGGAAAA‑AGATCCTGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTgg                                                                                                      <  1:476182/229‑1 (MQ=255)                                                                                                                     tttCCCAAAGGGGTT‑AA‑TGGTGGGGGTA‑TTGA‑TGCACACCCTTTTTTT‑C‑AGAAAACCTACGCTG‑TTTTA‑GTTGCAGAAGACAGAACATAAAAGGAAAA‑AGACCCTGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGa                                                                                                    <  2:523544/229‑1 (MQ=255)                                                                                                                     tttCCAAAAAGGTTT‑AA‑GGGTTGGGTGT‑TTTA‑T‑CTCTACCATTTGTT‑CAAGGAAACCT‑CCACGTATTTA‑CGTCCAGAGGTGGAAACATTAAAGGAAAA‑AGACACTGAAAATTATCAGAAAAAGGCCGCCACAAAGTTATTGGCGGTAGGCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCTTCATGGCAAGTC‑GTTAGGCTCTGGGa                                                                                                    <  2:713590/229‑1 (MQ=255)                                                                                                                     ttcccACAAAGGGTT‑GA‑TGGTTGGGCAT‑TATA‑TCCATA‑CACTGTTTTGC‑AGGAAACCTACGACG‑CTTAA‑TTTCCCGAAGTCGAGACATTAAAGGGAAA‑AGTTCCTGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGa                                                                                                    <  1:466420/226‑1 (MQ=255)                                                                                                                     ttcaccaaaggggTT‑AA‑GGGTGGGGCGT‑TAGA‑GGCACCTCACGTTTTA‑C‑AGGAAAGCGAGGAGT‑ATTTG‑TTTGCCAAAGTGGAGACATTAAAGGGAAA‑AGTTCCTAAAAATCATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCTCGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑TTTAGGCTCTGGGa                                                                                                    <  2:704017/219‑1 (MQ=255)                                                                                                                     gtcaccaaaaGGGTT‑AA‑TTGTGGGGC‑C‑TTTAGTCCCCAGCCCTTTTAT‑A‑AGAAAACCAGCGACT‑GTTTG‑GTTGCCGAAGGCGACAAATAAAAGGAAAA‑AGATCGTAAAAATTATCAGAAAAAGTCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGa                                                                                                    <  2:506308/222‑1 (MQ=255)                                                                                                                      tccctcaAAGGTGT‑AG‑TGGTTGGGTGT‑TACA‑TTCTCAGCCTTGTTTC‑A‑AGAAATCCGTCGACC‑TTTTA‑GTTGCGCAAGTCGAGACATAAAAGGGAAA‑GGCTCCAGAAAATTATCAGAAAAAGTCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGa                                                                                                    <  2:75508/223‑1 (MQ=255)                                                                                                                            aaaGGGTA‑AA‑TGGTTGGGCGT‑TATA‑TCGTAAGCCTTTTTTC‑A‑AGGAAACCAACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGAAAA‑ATATCCTAAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATaa                                                                                             <  1:301614/229‑1 (MQ=255)                                                                                                                                ggTT‑AACTGGTTGGGTGT‑ATTA‑TGTTCATCCCTTTTTT‑C‑AGTAAACCTACGACG‑TTTAA‑GTTGCCGAAGTCGAGA‑ATTTAAGGGAAATAGGTCCTGAAAATTAACAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATTGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGa                                                                                          <  1:35232/229‑1 (MQ=255)                                                                                                                                     aa‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAAGT‑ATTGAGGAGCATAATACGGTCAAACCAT‑CATCACCGGCCTTCCCGCCTGGGCAGATG‑TTTGGGCTCTGGG‑CAAATAAAAACTggg                                                                                     >  1:524614/1‑228 (MQ=255)                                                                                                                                      atgggTTGGGCGC‑TATA‑TCCTCAACCGTTTTTC‑A‑AGGAAACCAACGCCG‑CTTTG‑GTGGCCGAATCCGAGACATTAAAGGAAAA‑AGATCCGAAAAATTACCAGACAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGt                                                                                     <  2:624598/226‑1 (MQ=255)                                                                                                                                       gtGGTTGGGCAC‑TATC‑AGCTACTCCCTGGTTG‑A‑AAGAACCATACGCAG‑CTTGA‑ATTGCCGAAGGCGAGACAGTAAAGGGAAA‑AGGTCCTAAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑c                                                                                   <  1:34831/228‑1 (MQ=255)                                                                                                                                         ggTTGGGCTC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGAAAACCTACGAGG‑TTTTA‑GTTGCAGAAGTCGGGACATTAAAGGAAAA‑AGACCTTAAAAATTATCAGAGAAAAGCCGCCACAAAGTTAATGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CAc                                                                                 <  1:728126/229‑1 (MQ=255)                                                                                                                                              aaggacccga‑tGCTTTTCCCTGTTTA‑G‑AGGAAACCAACCACG‑ATTGA‑TGTGGCAAAGTCGAGAAATTAAAGGAAAA‑AGCCCCAGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGt                                                                             <  2:253052/220‑1 (MQ=255)                                                                                                                                               gccgttata‑TGTACACCCCTGTTTT‑A‑AGGAAACTTACGACA‑TATTA‑GCTGCAGAAAGGGAGAACTAAAAGGGAAA‑AAGTTCAAAAAATTATCAGAGAAAGGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTa                                                                            <  2:752300/224‑1 (MQ=255)                                                                                                                                                          gccaaaccttGTTTT‑A‑AGGAAACTTACGACG‑CTTAA‑GTTCCAGAATCCGAGACTTTAAAGGGAAA‑AGATCCTGAAAATTATAAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTg                                                                  <  1:219269/220‑1 (MQ=255)                                                                                                                                                          gCTCACCCGTGTTTT‑A‑AGGAACCCTTCGCTG‑ATTAA‑GTTGCCGAAGGAGAGCCATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGTAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTg                                                                  <  1:279643/229‑1 (MQ=255)                                                                                                                                                           gacATCACTGTTTT‑G‑AGGAAACCTACGAC‑‑CTTTAGGTTGCCAAGGTCGAGACATTAAGGGGAAA‑ATACCCTGAAAATTTTCAGAAAAAGTCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTg                                                                  <  1:174348/226‑1 (MQ=255)                                                                                                                                                               tCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAAAGAATTCAGAGGCTTA‑AACGGTGAATCCAT‑CATCACCCGCAAT‑ACGTCAAGGCAAAGC‑GCTTGGCTCCGGGTAAAAAAAAAAATCGT‑CACGGGGAAAAATTTGGGATGGt                                                             >  2:219269/1‑229 (MQ=255)                                                                                                                                                               tCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGGTGGCCATAAAATG‑ATTTAGGAGCATATCACGGTCAATCCAT‑AATCACCGGCAAT‑CCGTACCGGGAAGTCGGTAAGGCATGGGGAAAATTAAAAAC‑GGT‑TACGGGGAAAAATTgggtgtggt                                                             >  1:108607/1‑222 (MQ=255)                                                                                                                                                                 ccTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAAAG‑ATTGAGGAGCAAATCACGGTCAAACCAT‑CATCACCGGCATT‑CCCTCATGGCAAGGC‑GTTAGGCTCTGGGGAAAATAAAAACTGGT‑CACGGGGCAAATTTTGTGCTTGt                                                             >  1:529410/1‑227 (MQ=255)                                                                                                                                                                   tGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCCTAAAATG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑TCGTCCTGGCAAGTC‑GTTAGGCCCTGGGGAAAATAAAAAATGGG‑CACGGGGAAAAATTTGGGCTgtgtct                                                          >  1:253052/1‑223 (MQ=255)                                                                                                                                                                   tGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAAAG‑GTTGAGGAGCATATAACAGGCAATCACT‑CATCACCCGCCTT‑CCCTCATTGGAAGAC‑GTTTGGGGCTGGGGAAAAAAAAAACTAGTACCCGGGGAAAATTT‑GTGATGGGCGtt                                                         >  2:569612/1‑229 (MQ=255)                                                                                                                                                                   tGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGGCCCTAAAGAG‑ATTTAGGGGCATATCACGCGCAATCCAG‑CATCCCCCCCAAT‑CCGTCATTGCAAGAC‑GTTAGGGCCTGGGTAAAAAAAAAACAGGG‑CCCGGGGaaaaatagtggttggtgggt                                                         >  2:532081/1‑213 (MQ=255)                                                                                                                                                                     tttt‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGGGCATAAAATG‑ATTGAGGAGCATAACACGCTCAATACAT‑CATAACCGGCATT‑CCGTCATAGGAAACC‑GTTAGGCTCTGGGGAAAAAAAAAACTGGG‑CACCGGGAACAATTTGTGCTgggtgctat                                                       >  2:711865/1‑222 (MQ=255)                                                                                                                                                                     tttt‑A‑AGGAAACCTACGACG‑TTTTA‑GTTGCGGAAGTCGGAACATTAAAGGGAAA‑AGATCCTAAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGt                                                                                                                                                         <  2:246773/135‑1 (MQ=255)                                                                                                                                                                        t‑C‑AGGAAACCTACGACG‑CTTTA‑GTCGCCGAAGTCGAGACATTAAAGGGAAA‑ATATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGt                                                    <  1:507552/229‑1 (MQ=255)                                                                                                                                                                          taaGGAACCCTAGTACG‑TTTAA‑GTTGCCAAATGCAGGAAATAAAGGGGAAA‑AGACCCTAAAAATTATAAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGt                                                    <  1:227779/227‑1 (MQ=255)                                                                                                                                                                          taaGGAAAACTACCGCG‑TTTTA‑GGTGCCGAAGGCGAAAAATTAAGGGGAAA‑AGGTTCTGAAAAATAACAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGGCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGt                                                    <  2:290456/227‑1 (MQ=255)                                                                                                                                                                                          cgttttA‑GTTGCCGAAGTCGAGAAATTAAAGGGAAA‑AGACCCGGAAAATTATCAGAGAAAAGCCGGCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTta                                      <  1:77360/225‑1 (MQ=255)                                                                                                                                                                                            tgtttatgtggcGAAGTCGAAAAGTAAAAGGGAAA‑AGACCCTGAAAATTATCAGAGAAAGGCCGCCACAAAGTTATTGGCTGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTta                                      <  1:110922/216‑1 (MQ=255)                                                                                                                                                                                                         aagaggagaAATAAAAGGAAAA‑AGATCCGGAAAATAATAAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGaa                      <  2:740378/224‑1 (MQ=255)                                                                                                                                                                                                            tCGAGAAATTAAAGGAAAA‑AGATCCTGAAATTTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGt                   <  1:468815/229‑1 (MQ=255)                                                                                                                                                                                                              agaaCATTAAGGGAAAA‑AGATCGTGAAAATTAACAGGAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGtca                 <  1:711866/226‑1 (MQ=255)                                                                                                                                                                                                               agaCATTAAAGTGAAA‑AGACGCTGAAAATTAAAAGAGAAAACCCGCCCCAAAGTTATTGGCGGTAGTCCATAAAGTG‑AATGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGtca                 <  1:723252/228‑1 (MQ=255)                                                                                                                                                                                                                    taaaaGGGAAA‑ATATCCAGAAAATGAAAAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGCCCATAAAGGG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCAtt            <  2:529410/226‑1 (MQ=255)                                                                                                                                                                                                                    taaaaGGAAAA‑AGACCCTAAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCAtt            <  1:636275/226‑1 (MQ=255)                                                                                                                                                                                                                     taaagaggaaaaagccgaaaaaaTTAGCAGAGAAAAACCGCCACAAAGTTATTGGGGGGAAGCCATAAAGGG‑ATTGAGGAGCATATCACGGGCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCGCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTc           <  2:576610/210‑1 (MQ=255)                                                                                                                                                                                                                     tAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTTGTGCATCAAAGG‑ATTTAGGGGGCAATCACGGACAAATCATCCATC‑CCGGCGTT‑CCGTCTTTGCAAGTC‑CGTAGGCTTGGGGAAAAAACAAAAATGGG‑CACGGGGAAAAATTTGTGGACGTCGTTGACGTCCATTATTTTTTTTTAGTTGAAAAAGGAAAAgaaccaagta           >  2:495101/1‑220 (MQ=255)                                                                                                                                                                                                                             aa‑aGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGACCCTAAAATA‑GTTGGGGAGCAAATCACGGTCAATCCAT‑CCTCACCGGCATT‑CCTTCATGGCGAGTA‑GTTAGGCTCTGGGCAACATCAAGACTGGG‑GACGGGGAAACATTTGTGCTGGGCGTTATCGTTTCTTCTTTCGTTTTAA‑TGAAAAAAAGAAAAGTATCATTCTGggatggg  >  1:421645/1‑228 (MQ=255)                                                                                                                                                                                                                                     cTGAAAATTAGCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTg                                                                  <  1:65512/159‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                          ATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATT‑GGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGA  >  NC_000913/3277226‑3277665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑