Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,277,497 G→A 14.4% V55M (GTG→ATG)  yhaV → toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,277,4970GA14.4% 173.8 / 3.8 73V55M (GTG→ATG) yhaVtoxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (3/58);  new base A (9/2);  total (12/61)
Fisher's exact test for biased strand distribution p-value = 1.30e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

AAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATA  >  NC_000913/3277275‑3277716
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 
aaagaacttggtgtggggaaTGGACGTAAACTTTGAGTTGGATATTGGCGCCGACTATTCATGGATTTCCCACAGAGGTTA‑AA‑GGGTTGGGCGT‑TATA‑TTCTCATCCCTTGTTG‑C‑AGGAAACCAACGACT‑CTTTA‑TTTGCCAAAGTCGATACATTAAAGGGAAA‑ATATCCTGAAAATTATCAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTg                                                                                                                                                                                                                            <  1:741621/210‑1 (MQ=255)
  agaaaCTTTGCGCTGGCAGGGACGTAAACAGTGATGATGGGATGGGACACAGCGAATAAGGGATTTTCCACAAGGGGTT‑AA‑TGGTTGGGGGT‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATAATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTgag                                                                                                                                                                                                                          <  2:412583/229‑1 (MQ=255)
    aatcTTGTCGCTGGCAAGGATGTCAACATTGATGATGAGGTTGGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGA‑TATA‑ATCTCATCCTTGTTTA‑C‑AGGAAACCTACGACG‑TTTTA‑GTTGCCGAAGCAGAAACATTAAAGGAAAA‑AGATCCTGAAAATTAACAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTgagga                                                                                                                                                                                                                        <  1:149928/226‑1 (MQ=255)
            cGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTCCGACG‑ATTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCa                                                                                                                                                                                                                <  1:53544/229‑1 (MQ=255)
                      tgcGTAAACATGGATGAGGAGATGGACGACGACGAATAATGGCTTCTGCACAAGGGGTT‑AA‑TGGTTGGGCAC‑TTGA‑CGCTATTCCCGGTTTT‑A‑AGGAAACCTACGCAC‑ATTAA‑GTAAGCGAAGTCGATACATTAAAGGAAAA‑AGACCATGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATcc                                                                                                                                                                                                      <  1:342149/227‑1 (MQ=255)
                                        gagaTTGGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCCTAAAGAA‑CATGAGGGGCAAATCACGGTCAATACAT‑CATCAACGGCATT‑CCg                                                                                                                                                                                  >  1:624599/1‑229 (MQ=255)
                                             tGGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCAGCCACAAAGTTATTGGCGGTAGACAATAAAGGG‑ATTTAGGGGCATAACACGGGCAAACcacacataccgtcattccctcctg                                                                                                                                                                               >  1:290456/1‑207 (MQ=255)
                                                gcaccacaaaactttgtttttCCCAAAATGGTT‑AA‑GGGTTGTGCTC‑TAGA‑TCTACATCCCTTTTTT‑A‑TAAAACCCGACGACA‑TTTTA‑TTTCCAAAGGTCGAAACTTAAAAGGCAAA‑GGACCCTAAAAATTCTCTGAAAAAT‑GCCGCCCCAAAGTTATTGGCGGTAGCCCATAAAGTG‑ATTTAGGAGCATATCGCGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGcg                                                                                                                                                                          <  2:386779/212‑2 (MQ=255)
                                                ctaggaagaATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGTGA‑TAAA‑TGCTCACCCTTGTTTG‑C‑AGGAAACCTACGCCG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCa                                                                                                                                                                          <  2:160865/222‑1 (MQ=255)
                                                 tgagtctaataatGGGTTTCCCCAAAGGGGTT‑AA‑TGG‑TGGGCGCGTAAG‑CGCTCATCCCTGTTTC‑C‑AAGAACCCGACGACG‑ATTTA‑GTTGAAGAAGTCGAGAAATAAAGGGAAAA‑AGAGCCTGAAAAGTACAAGAGAAAA‑GCCGCCACAAAGGTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGAATT‑CCGTCATGGCa                                                                                                                                                                          <  1:350867/221‑1 (MQ=255)
                                                 cggcgaccaattagggttttCCCCAAAAGGTTGAA‑TGGTTGCGCGT‑TATC‑GGGTCATCCCTCTTTC‑C‑AGGAAACTAACGGCG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTAACAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCa                                                                                                                                                                          <  1:366521/213‑1 (MQ=255)
                                                      ccaataagGGATTTCCCAAAAGGGGTT‑AA‑TGGTTGGGCGC‑TTAA‑TGCTAACCCTTGTTTT‑C‑AGGAAACCTACGACG‑TTTAA‑GTACCCAAAGCCGAGAATTTAAAGGAAAA‑AGACCCTGAAAATTATCAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑Gt                                                                                                                                                                   <  1:202446/227‑1 (MQ=255)
                                                       aaataatGGATTTCCCACAAAGGGTT‑AA‑GGGTTGGGCGC‑TATA‑TGCTCATCCGTTTTTT‑C‑AGGAAACTTACGACG‑TTTTA‑GTTGCCGAAGCCGAGAAATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑Gtt                                                                                                                                                                  <  1:521007/228‑1 (MQ=255)
                                                             ttgATTTTCCAAAAAGGTTA‑AA‑GGGTTGGGCTC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTGCGACG‑TTTTA‑GTTGCCAAAGTCGAAAAATTAAAGGGAAA‑ATACCCTGAAAATTATCAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGct                                                                                                                                                             <  1:647480/226‑1 (MQ=255)
                                                                aTTTCCCAAAAGGGGTT‑AA‑GGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑A‑AGGAAACCTCCGACT‑CTTTA‑GTTGCCGAAGTCGAGACATAAAAGGAAAA‑AGATCCTGAAAATTATCAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTgg                                                                                                                                                         <  1:476182/229‑1 (MQ=255)
                                                                  tttCCCAAAGGGGTT‑AA‑TGGTGGGGGTA‑TTGA‑TGCACACCCTTTTTTT‑C‑AGAAAACCTACGCTG‑TTTTA‑GTTGCAGAAGACAGAACATAAAAGGAAAA‑AGACCCTGAAAATTATCAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGa                                                                                                                                                       <  2:523544/229‑1 (MQ=255)
                                                                  tttCCAAAAAGGTTT‑AA‑GGGTTGGGTGT‑TTTA‑T‑CTCTACCATTTGTT‑CAAGGAAACCT‑CCACGTATTTA‑CGTCCAGAGGTGGAAACATTAAAGGAAAA‑AGACACTGAAAATTATCAGAAAAAG‑GCCGCCACAAAGTTATTGGCGGTAGGCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCTTCATGGCAAGTC‑GTTAGGCTCTGGGa                                                                                                                                                       <  2:713590/229‑1 (MQ=255)
                                                                  ttcccACAAAGGGTT‑GA‑TGGTTGGGCAT‑TATA‑TCCATA‑CACTGTTTTGC‑AGGAAACCTACGACG‑CTTAA‑TTTCCCGAAGTCGAGACATTAAAGGGAAA‑AGTTCCTGAAAATTATCAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGa                                                                                                                                                       <  1:466420/226‑1 (MQ=255)
                                                                  ttcaccaaaggggTT‑AA‑GGGTGGGGCGT‑TAGA‑GGCACCTCACGTTTTA‑C‑AGGAAAGCGAGGAGT‑ATTTG‑TTTGCCAAAGTGGAGACATTAAAGGGAAA‑AGTTCCTAAAAATCATCAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCTCGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑TTTAGGCTCTGGGa                                                                                                                                                       <  2:704017/219‑1 (MQ=255)
                                                                  gtcaccaaaaGGGTT‑AA‑TTGTGGGGC‑C‑TTTAGTCCCCAGCCCTTTTAT‑A‑AGAAAACCAGCGACT‑GTTTG‑GTTGCCGAAGGCGACAAATAAAAGGAAAA‑AGATCGTAAAAATTATCAGAAAAAG‑TCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGa                                                                                                                                                       <  2:506308/222‑1 (MQ=255)
                                                                   tccctcaAAGGTGT‑AG‑TGGTTGGGTGT‑TACA‑TTCTCAGCCTTGTTTC‑A‑AGAAATCCGTCGACC‑TTTTA‑GTTGCGCAAGTCGAGACATAAAAGGGAAA‑GGCTCCAGAAAATTATCAGAAAAAG‑TCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGa                                                                                                                                                       <  2:75508/223‑1 (MQ=255)
                                                                         aaaGGGTA‑AA‑TGGTTGGGCGT‑TATA‑TCGTAAGCCTTTTTTC‑A‑AGGAAACCAACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGAAAA‑ATATCCTAAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATaa                                                                                                                                                <  1:301614/229‑1 (MQ=255)
                                                                             ggTT‑AACTGGTTGGGTGT‑ATTA‑TGTTCATCCCTTTTTT‑C‑AGTAAACCTACGACG‑TTTAA‑GTTGCCGAAGTCGAGA‑ATTTAAGGGAAATAGGTCCTGAAAATTAACAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATTGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGa                                                                                                                                             <  1:35232/229‑1 (MQ=255)
                                                                                  aa‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAAGT‑ATTGAGGAGCATAATACGGTCAAACCAT‑CATCACCGGCCTTCCCGCCTGGGCAGATG‑TTTGGGCTCTGGG‑CAAATAAAAACTggg                                                                                                                                        >  1:524614/1‑228 (MQ=255)
                                                                                   atgggTTGGGCGC‑TATA‑TCCTCAACCGTTTTTC‑A‑AGGAAACCAACGCCG‑CTTTG‑GTGGCCGAATCCGAGACATTAAAGGAAAA‑AGATCCGAAAAATTACCAGACAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGt                                                                                                                                        <  2:624598/226‑1 (MQ=255)
                                                                                    gtGGTTGGGCAC‑TATC‑AGCTACTCCCTGGTTG‑A‑AAGAACCATACGCAG‑CTTGA‑ATTGCCGAAGGCGAGACAGTAAAGGGAAA‑AGGTCCTAAAAATTATCAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑c                                                                                                                                      <  1:34831/228‑1 (MQ=255)
                                                                                      ggTTGGGCTC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGAAAACCTACGAGG‑TTTTA‑GTTGCAGAAGTCGGGACATTAAAGGAAAA‑AGACCTTAAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTAATGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CAc                                                                                                                                    <  1:728126/229‑1 (MQ=255)
                                                                                           aaggacccga‑tGCTTTTCCCTGTTTA‑G‑AGGAAACCAACCACG‑ATTGA‑TGTGGCAAAGTCGAGAAATTAAAGGAAAA‑AGCCCCAGAAAATTATCAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGt                                                                                                                                <  2:253052/220‑1 (MQ=255)
                                                                                            gccgttata‑TGTACACCCCTGTTTT‑A‑AGGAAACTTACGACA‑TATTA‑GCTGCAGAAAGGGAGAACTAAAAGGGAAA‑AAGTTCAAAAAATTATCAGAGAAAG‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTa                                                                                                                               <  2:752300/224‑1 (MQ=255)
                                                                                                       gccaaaccttGTTTT‑A‑AGGAAACTTACGACG‑CTTAA‑GTTCCAGAATCCGAGACTTTAAAGGGAAA‑AGATCCTGAAAATTATAAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTg                                                                                                                     <  1:219269/220‑1 (MQ=255)
                                                                                                       gCTCACCCGTGTTTT‑A‑AGGAACCCTTCGCTG‑ATTAA‑GTTGCCGAAGGAGAGCCATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGTAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTg                                                                                                                     <  1:279643/229‑1 (MQ=255)
                                                                                                        gacATCACTGTTTT‑G‑AGGAAACCTACGAC‑‑CTTTAGGTTGCCAAGGTCGAGACATTAAGGGGAAA‑ATACCCTGAAAATTTTCAGAAAAAG‑TCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTg                                                                                                                     <  1:174348/226‑1 (MQ=255)
                                                                                                            tCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAAAGAATTCAGAGGCTTA‑AACGGTGAATCCAT‑CATCACCCGCAAT‑ACGTCAAGGCAAAGC‑GCTTGGCTCCGGGTAAAAAAAAAAATCGT‑CACGGGGAAAAATTTGGGATGGt                                                                                                                >  2:219269/1‑229 (MQ=255)
                                                                                                            tCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGGTGGCCATAAAATG‑ATTTAGGAGCATATCACGGTCAATCCAT‑AATCACCGGCAAT‑CCGTACCGGGAAGTCGGTAAGGCATGGGGAAAATTAAAAAC‑GGT‑TACGGGGAAAAATTgggtgtggt                                                                                                                >  1:108607/1‑222 (MQ=255)
                                                                                                              ccTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAAAG‑ATTGAGGAGCAAATCACGGTCAAACCAT‑CATCACCGGCATT‑CCCTCATGGCAAGGC‑GTTAGGCTCTGGGGAAAATAAAAACTGGT‑CACGGGGCAAATTTTGTGCTTGt                                                                                                                >  1:529410/1‑227 (MQ=255)
                                                                                                                tGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCCTAAAATG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑TCGTCCTGGCAAGTC‑GTTAGGCCCTGGGGAAAATAAAAAATGGG‑CACGGGGAAAAATTTGGGCTgtgtct                                                                                                             >  1:253052/1‑223 (MQ=255)
                                                                                                                tGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAAAG‑GTTGAGGAGCATATAACAGGCAATCACT‑CATCACCCGCCTT‑CCCTCATTGGAAGAC‑GTTTGGGGCTGGGGAAAAAAAAAACTAGTACCCGGGGAAAATTT‑GTGATGGGCGtt                                                                                                            >  2:569612/1‑229 (MQ=255)
                                                                                                                tGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGGCCCTAAAGAG‑ATTTAGGGGCATATCACGCGCAATCCAG‑CATCCCCCCCAAT‑CCGTCATTGCAAGAC‑GTTAGGGCCTGGGTAAAAAAAAAACAGGG‑CCCGGGGaaaaatagtggttggtgggt                                                                                                            >  2:532081/1‑213 (MQ=255)
                                                                                                                  tttt‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGGGCATAAAATG‑ATTGAGGAGCATAACACGCTCAATACAT‑CATAACCGGCATT‑CCGTCATAGGAAACC‑GTTAGGCTCTGGGGAAAAAAAAAACTGGG‑CACCGGGAACAATTTGTGCTgggtgctat                                                                                                          >  2:711865/1‑222 (MQ=255)
                                                                                                                  tttt‑A‑AGGAAACCTACGACG‑TTTTA‑GTTGCGGAAGTCGGAACATTAAAGGGAAA‑AGATCCTAAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGt                                                                                                                                                                                                            <  2:246773/135‑1 (MQ=255)
                                                                                                                     t‑C‑AGGAAACCTACGACG‑CTTTA‑GTCGCCGAAGTCGAGACATTAAAGGGAAA‑ATATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGt                                                                                                       <  1:507552/229‑1 (MQ=255)
                                                                                                                       taaGGAACCCTAGTACG‑TTTAA‑GTTGCCAAATGCAGGAAATAAAGGGGAAA‑AGACCCTAAAAATTATAAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGt                                                                                                       <  1:227779/227‑1 (MQ=255)
                                                                                                                       taaGGAAAACTACCGCG‑TTTTA‑GGTGCCGAAGGCGAAAAATTAAGGGGAAA‑AGGTTCTGAAAAATAACAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGGCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGt                                                                                                       <  2:290456/227‑1 (MQ=255)
                                                                                                                                       cgttttA‑GTTGCCGAAGTCGAGAAATTAAAGGGAAA‑AGACCCGGAAAATTATCAGAGAAAA‑GCCGGCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTta                                                                                         <  1:77360/225‑1 (MQ=255)
                                                                                                                                         tgtttatgtggcGAAGTCGAAAAGTAAAAGGGAAA‑AGACCCTGAAAATTATCAGAGAAAG‑GCCGCCACAAAGTTATTGGCTGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTta                                                                                         <  1:110922/216‑1 (MQ=255)
                                                                                                                                                      aagaggagaAATAAAAGGAAAA‑AGATCCGGAAAATAATAAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGaa                                                                         <  2:740378/224‑1 (MQ=255)
                                                                                                                                                         tCGAGAAATTAAAGGAAAA‑AGATCCTGAAATTTATCAGAAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGt                                                                      <  1:468815/229‑1 (MQ=255)
                                                                                                                                                           agaaCATTAAGGGAAAA‑AGATCGTGAAAATTAACAGGAAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGtca                                                                    <  1:711866/226‑1 (MQ=255)
                                                                                                                                                            agaCATTAAAGTGAAA‑AGACGCTGAAAATTAAAAGAGAAAA‑CCCGCCCCAAAGTTATTGGCGGTAGTCCATAAAGTG‑AATGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGtca                                                                    <  1:723252/228‑1 (MQ=255)
                                                                                                                                                                 taaaaGGGAAA‑ATATCCAGAAAATGAAAAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGCCCATAAAGGG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCAtt                                                               <  2:529410/226‑1 (MQ=255)
                                                                                                                                                                 taaaaGGAAAA‑AGACCCTAAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCAtt                                                               <  1:636275/226‑1 (MQ=255)
                                                                                                                                                                  taaagaggaaaaagccgaaaaaaTTAGCAGAGAAAA‑ACCGCCACAAAGTTATTGGGGGGAAGCCATAAAGGG‑ATTGAGGAGCATATCACGGGCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCGCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTc                                                              <  2:576610/210‑1 (MQ=255)
                                                                                                                                                                  tAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTTGTGCATCAAAGG‑ATTTAGGGGGCAATCACGGACAAATCATCCATC‑CCGGCGTT‑CCGTCTTTGCAAGTC‑CGTAGGCTTGGGGAAAAAACAAAAATGGG‑CACGGGGAAAAATTTGTGGACGTCGTTGACGTCCATTATTTTTTTTTAGTTGAAAAAGGAAAAgaaccaagta                                                              >  2:495101/1‑220 (MQ=255)
                                                                                                                                                                          aa‑aGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGACCCTAAAATA‑GTTGGGGAGCAAATCACGGTCAATCCAT‑CCTCACCGGCATT‑CCTTCATGGCGAGTA‑GTTAGGCTCTGGGCAACATCAAGACTGGG‑GACGGGGAAACATTTGTGCTGGGCGTTATCGTTTCTTCTTTCGTTTTAA‑TGAAAAAAAGAAAAGTATCATTCTGggatggg                                                     >  1:421645/1‑228 (MQ=255)
                                                                                                                                                                                  cTGAAAATTAGCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTg                                                                                                                     <  1:65512/159‑1 (MQ=255)
                                                                                                                                                                                            tgagagAAAA‑GCCGCCACAAATGTATTGGCGGTCGTCCAGAAAGTG‑ATTGAGGGTGATATCACGGTCAATCGAT‑CATCACCGGCATC‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACAct                                    <  1:359966/227‑1 (MQ=255)
                                                                                                                                                                                             acgagaAAAGGCCGCCACAATGGTATTCGCGGTACTCCATAAAGTG‑ATGGAGGAGCATATCACTGTCAATACCT‑CATAACCGGCCTT‑CGGTCAGGAAAAGTT‑GGTAGGCTCTGGCAAAAATAAAGGCTGGG‑CTGGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCGTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAacaaa                                    <  2:579833/227‑3 (MQ=255)
                                                                                                                                                                                               gaaaaaa‑GCCGCCACAAAGGTATTGGCGGTATTCCTAAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTg                                 <  2:571664/226‑1 (MQ=255)
                                                                                                                                                                                                   aaaggccgcccacacgTTATTTGCGGTAGTCCAGAAAGTG‑ATTGAGGCGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTGAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTgcgc                              <  1:724928/214‑1 (MQ=255)
                                                                                                                                                                                                                     tttGGTGGTTGTCCATAAACTT‑ATTTGGGCGTATCTCATGGTCAATCCAT‑CATCACCGGCAGT‑CCGTCAGGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATGTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGACCGATGAAAACACTCTGCGCACCTCCGGTAAAAAAACa            <  2:490594/228‑1 (MQ=255)
                                                                                                                                                                                                                     tttGGCTGTAGTCCATAAAAGT‑ATTGGCGAGCATACCACGGTCAATCCAT‑CAACACCGGCATT‑CCGTCAGGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CATGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTGCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa            <  2:108607/228‑1 (MQ=255)
                                                                                                                                                                                                                     cttGGCGGTGGTACAAAAAGGG‑ATGGAGGAGAATATCACGGGCAAACCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGTTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa            <  2:709899/228‑1 (MQ=255)
                                                                                                                                                                                                                      ttGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACa            <  1:445417/228‑1 (MQ=255)
                                                                                                                                                                                                                      ttGGCGGTAGGCCATAAAGGG‑ATTGAGGGGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAg           <  1:451466/229‑1 (MQ=255)
                                                                                                                                                                                                                      atGGCGGTAGTCCATAAAGGT‑ATTGAGGAGCATATCACGGTCCATCCAT‑CAGCACCGGCATG‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCCCCTACGGTAAAAAAACAg           <  1:725052/228‑1 (MQ=255)
                                                                                                                                                                                                                       tGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CAGCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGa          <  1:651521/229‑1 (MQ=255)
                                                                                                                                                                                                                       tGGCGGTAGTCCAAAAAATT‑AGTGTGGGTGATAACACGGTCAATCCAA‑CAGCCCCGGCATT‑CCGTGATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGa          <  2:62319/229‑1 (MQ=255)
                                                                                                                                                                                                                       gggCGGTAGGCCATGAAGTG‑GGTGAGGAGCATTTCACGGTAAATCCAC‑CATCCGCGGCATT‑CCGTCATGGCAAGTC‑GTTTGGCTCTGGGAAAAATAAAGACTGTG‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGa          <  2:116363/228‑1 (MQ=255)
                                                                                                                                                                                                                          gcggttcccgtAAAGTT‑GTTGAGGAGCATTTCACGGTCAAGCCCT‑CAACGACGGCTCT‑ACTACATGGCCACTC‑TCTAGTCTCTGGTAAAAATAAAGACTGTT‑CGCGTGTAAAATTTGGTGCTGGTCGTTAGCGTCTCTTCTTGCGTGATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGACCGATTAAAACACTCTGCCCACCTACGGTAAAAAAACAGATGc       <  2:525557/219‑1 (MQ=255)
                                                                                                                                                                                                                          cGGTAGTCCATAAAGTG‑ATTGATGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGc       <  1:676909/229‑1 (MQ=255)
                                                                                                                                                                                                                          cGGTAGTCCATAAAGGG‑ATTGAGGAGCATATTACGGTAAAGCCAT‑CAACACCGGCATC‑CCGGCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGc       <  2:44349/229‑1 (MQ=255)
                                                                                                                                                                                                                            gttgTCCATAAAGTT‑ATTGGGGAGCATAATGCGGTCACTCCAT‑CAACACCGGCATT‑TCGTCCTGGCAAGGT‑GTTAGGCTCTGGGAAAAATAAAGACTTGT‑CCCGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTGTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGCAATCACGCTGCGCACCTACGGTAAAAAAACAGATGCCt     <  2:512527/226‑1 (MQ=255)
                                                                                                                                                                                                                               gTCCATAAAGTG‑ATTTAGGAGCATATAACGGTTCATCCAT‑CAAACCCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTTCTGGTCGGTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCtata  <  2:47639/229‑1 (MQ=255)
                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 
AAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGGTT‑AA‑TGGTTGGGCGC‑TATA‑TGCTCATCCCTGTTTT‑C‑AGGAAACCTACGACG‑CTTTA‑GTTGCCGAAGTCGAGACATTAAAGGGAAA‑AGATCCTGAAAATTATCAGAGAAAA‑GCCGCCACAAAGTTATTGGCGGTAGTCCATAAAGTG‑ATTGAGGAGCATATCACGGTCAATCCAT‑CATCACCGGCATT‑CCGTCATGGCAAGTC‑GTTAGGCTCTGGGAAAAATAAAGACTGGT‑CACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAG‑TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATA  >  NC_000913/3277275‑3277716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑