| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,749,428 | T→G | 20.3% | G66G (GGT→GGG) | sgbU → | putative L‑xylulose 5‑phosphate 3‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,749,428 | 0 | T | G | 20.3% | 94.5 / 3.3 | 65 | G66G (GGT→GGG) | sgbU | putative L‑xylulose 5‑phosphate 3‑epimerase |
| Reads supporting (aligned to +/- strand): ref base T (21/26); new base G (14/2); total (37/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.39e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCGACGCTATCTGGGGAGGTGCGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACA > NC_000913/3749205‑3749655 | aaacgacgaTTTCTGGGGAGTTGCGCGTGCGTAATCATCATGTAGGGAGTGATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTCTGATTTTGTCGAAATGACGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTgg < 1:583298/226‑1 (MQ=255) aTCGACGCTATCTGGGGAGGTGCGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGCCTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGGGGTggg > 1:658128/1‑229 (MQ=255) tccgcTGGGGGGGTGGGTGTGCGTAATAAGCGGTGTGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGt < 2:315655/225‑1 (MQ=255) tttgtGGGGGGGTGGGCGTGGGTAATCCTCAGTTAGGGATTTATGAAAAAGCGGTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTTCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCGCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGt < 2:51853/224‑1 (MQ=255) tCTGGGGAGGTGCGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGAGCccgccg > 1:400179/1‑228 (MQ=255) ggAGGTGCGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGGGTTGGGATCCCGTCGATGTGc > 1:369919/1‑229 (MQ=255) ggAGGTGCGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGGCGGTGGATGAAACCGCCGAACGGCTCTCCCTTCTTGACTGGGACGCCCCTCAACA‑GACTTCCGTGGTTGCCCCGA‑TTGTCCAAACCGGGGTTggggaccctgcgacgtc > 2:710358/1‑214 (MQ=255) tGCGCGTGCGTAATCATCAGTTAGGGATTTATGGAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGGGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGt > 1:237827/1‑228 (MQ=255) tGCGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGTCCGGAGCGGCTGGGGCTGGCACAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAACCCGACGCACGCGCCTCACGTCTTGACTGGAGCGCCGGCCCAAG‑GACTTCCTCGGGTGCC‑CGAGTGATCGAAACAGGGGCTGGGAGCCCGTCCTTGGGCCttg > 2:614260/1‑226 (MQ=255) tGCGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGGTGAAACCGACGAACGGGTCTCACGTCTTGACTGGAGGGCCGCACAAAG‑GACTTCCCTGGTTGCCGCGT‑GTTTCGAAACAGGGGTTTGGAACCCGAGGAGGGGCCTGtt > 2:667663/1‑228 (MQ=255) tGCGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGGGTTGGGATCCCGTCGATGTGCCTGt > 1:188302/1‑228 (MQ=255) tGCGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGGGTTGGGATCCCGTCGATGTGCCTGt > 1:626675/1‑228 (MQ=255) cgcgTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGGGCCTgcggt > 1:315655/1‑225 (MQ=255) cgcgTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGGGTTGGGATCCCGTCGAGGTGCCTGTCCg > 1:105269/1‑229 (MQ=255) cgcgTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTATCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGGGTTGGGATCCCGTCGATGTGCCTTTcc > 1:419014/1‑228 (MQ=255) cgtgcgtAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGAGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGCCGGGGGTTGAAAACGACGAACGGCCCCCACCGCTTGACTGGAGCCGCGCCCAAAG‑GACCTCCCGGGTTGTCGCGA‑TGCATGAAACAGGTGTCGGGAAccccccggaggggcctgccccc > 2:509178/1‑210 (MQ=255) gTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGAGTTGGGATCCCGTCCATTTGCCTTTCCCCACATCCTCGCTTTccc > 1:364190/1‑229 (MQ=255) tttATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGGTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCCCGC‑TGGTCGAAACAGGGGTTGGGAGCCCGTCGATGGGCCTGGCCGCCAATTGTCGCTTTCCCTTTGGGAg > 2:548458/1‑229 (MQ=255) ttATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGGTTTGGGAGCCCGTCGAGTGGCCTGTCGGCACATCGTCGCTTTCCCTTTgtaat > 1:413773/1‑224 (MQ=255) ttATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCCGGTTGCCCCGA‑GTACTGAAACCGGTGTTGGGAGCCCGTCGATGTGCCCTGCCGCACATCCGCGCTTTCCCCTTTGGAGc > 2:503520/1‑229 (MQ=255) aTGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGCTCGAAACAGGGGTTGGGATCCCGTCGATGGGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCg > 1:730792/1‑229 (MQ=255) gcgcGGGCGGAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGGTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAg‑g < 1:657124/229‑1 (MQ=255) gggCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCGGGCAAAAAGCTGCGGTTTTGATTTTGGCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGt < 1:502672/228‑1 (MQ=255) gCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCATGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATccc < 2:317872/153‑1 (MQ=255) gCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGCAAACGGGGTTGGGATCCCGTCGATGTGCCTGTCCGCACCACGTCCCTTTCCCTTTGGTGGCCCGGGAGGG‑GCAGGGGGCGAACGGGCGCGGGaa > 2:133128/1‑229 (MQ=255) gCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATccc > 1:317872/1‑153 (MQ=255) gCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGGG‑GCAGTGCGCGAACGGGCGCGGGaa > 1:651654/1‑229 (MQ=255) ggtggctggggctggCAAAAAGCCGCGGTGTTGATTTGGGCGGAGTGGCGGGGGATGAAAGCGACGAACGGCTCTCGCCTCTTGACTGGAGCGCCGCTCAAAG‑GACTTCGCTGGTTGCCGCGA‑TCATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCAt < 2:140970/226‑1 (MQ=255) aGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGGAATCAt > 1:231657/1‑229 (MQ=255) aGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCCTCGATGTGCCTGTCCGCCCATCGTCGCTTTCCCTTTGGTAGCCGTGACCAGCGCATGGCCCAAACGGCCG‑GGGAAACCAt > 2:163112/1‑229 (MQ=255) cTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGCGTTGGGATCCCGTCGATGTGCCTGTCCGACCATCGTCGCTTTCCCTTTGGGAGCCGTGACGAG‑GCAGTGCGCGAACGGGGGCGGGAAATCATggga > 2:175271/1‑225 (MQ=255) ggTTCTGGCAAAAAGCTGCCGTTTTGCTTTTGTCGAAATGTCGGTGGATGAAACCGGCGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTaaa < 1:667664/229‑1 (MQ=255) ggTGTTGGCAAAAAGGTTGGGTTTTGATTTTTGCGAAATGTCGGTGAATGAAACCGACGAACGGCTCTCGCGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTaa < 2:237827/228‑1 (MQ=255) aaaagggttcGGTTTTGATTTTGTCGAAATGTCGGTGGATGAACACGACGAACGGCTCTCACGGCTTGACCGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGc < 2:105269/220‑1 (MQ=255) aaGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCCTTACCTTTGGTCGCCCTGCCGAG‑GCAGTGCGCGACCGGGCGCGGGACATCATGAGGAAAACGATTGGCGTg > 2:508316/1‑229 (MQ=255) tGCGGTTTTGTGTTTGTCGAAATTGGGGTGGAGGAAACCGACGAATGGCTCTCCCGTCTTGACTGGAGCGGCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGgcgc < 2:730791/229‑1 (MQ=255) gggtgtttGTTTTGATCGAAATTGGGGTGGGTGAAACCGACTAACGGCCGTGACGTCTTGTCGGGCGCGCCGCTGAAAG‑GACTTCGCTGGTTGCCGCGA‑ATAGCGAAAGAGGTGTGTGGATCCCGTGGATGTGCCTGTCCGCACATCGTCGTTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCTCGGGAAATCATGAGTAAAGCGATTCGCCTGgcgcg < 2:247584/224‑1 (MQ=255) gCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGCACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGgcg > 1:598139/1‑227 (MQ=255) gCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGGGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCCCGAACGGGCGCGGGAAAACATGAGTAAAGCGATTCGCCTGgcggg > 2:707338/1‑227 (MQ=255) aTTGTGGCGAAATGTCGGTGGATGAAACCGACGAACGGTTCTCACGTCTTGACTGGATCGCCGCACAAAG‑GACGTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGGTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑Atctc < 1:66796/227‑1 (MQ=255) gtggtgggctGAAACCGACGAAGGGCTCCCCCGTCTTGACTGGGGCGCCGCACAAGG‑GCCTTCGCGGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATc < 2:704707/220‑1 (MQ=255) ggTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGC‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTCGCCGTGACTAG‑GCATTCCGCAGACGGGCCCGGGAAACCCTCGGAAAACGGATTCGCCACGC‑CGCGCATCCCGGCATTAGcccacaccga > 2:709971/1‑220 (MQ=255) gATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGGGACGAG‑GCAGTCGGCGAACGTGCGCGGGAAATAATGAGCAAAGCGAGTCGCCTGGCGCGCG‑ATCTCGGGCTTCGGACCATCCCACTgg > 2:659350/1‑229 (MQ=255) aTGAAACGGACGGACGGCTTTGACGTCTTGACTGGGTCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACAGCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGc < 2:626674/229‑1 (MQ=255) aaCCGACGAACGGCTCTCACGTCTTTACTGGCGCTCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAgg < 1:614261/228‑1 (MQ=255) gacgaACGGCTCTGACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTAt < 1:139314/228‑1 (MQ=255) gacgaACGGCTCTCCCGTCTGGACTGGAGCGCCGCACAAAG‑GCCTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTAt < 1:556133/228‑1 (MQ=255) acgaACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGCAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑GTCTCGGCATTCGCCCCGTCCCGCTTGACGGTTTTGa > 1:285103/1‑229 (MQ=255) acgaACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGCTATGa > 1:602772/1‑229 (MQ=255) ctagcGGCTCTCCCGTTTTGACTGGGTCGCTGCCCAAGG‑GACTTCTCTGGTTTGCGGCA‑TGAACGGAACGGGTGTTGGGAGCCCGTCGATGTGGCTGGCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGa < 2:457961/224‑1 (MQ=255) cgaACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTTTGAc > 1:659715/1‑229 (MQ=255) ggagggCTCTCCCGTCTTGGAGGGAGGGGCG‑GCAAAGTGACTTCGCTGGGTGCCGCTT‑GGATCGGAACAGGTGTTGGGATCCCGTCGAGGTGCCTGTCCGCGCATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGGGGGAAATCATGAGTAAAGCGATTCGACTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGAc < 2:364190/224‑1 (MQ=255) gaACGGCCTTCACGGTTTGACTGGAGCGCCGGACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGGCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGAc < 2:651653/228‑1 (MQ=255) ggAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACCGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCAcgacga < 1:133128/229‑1 (MQ=255) gcgcgccaaaggagctgcgttgTTGGCGGGA‑TGAACCAAACAGGTTTTGGGAATCCGTGCAGGGGCCTGGCCGCACATCGGCGGTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCa < 2:419014/208‑1 (MQ=255) cacaAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCCCGCCGCAGGCACCCg > 1:207091/1‑229 (MQ=255) aaaG‑GCCTTGGCGTGTTGCCGCGA‑TGATCGAAACAGGTGTGGGGATCCCGTCGATGTGCCTGGCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGc < 2:658127/228‑1 (MQ=255) ttCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGCGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCGCCATCCAGCTGGCAGGTTATGACGTCTGTTCCGAAGACCACGACGGAGGCACCCGGCAACGtttt > 1:302190/1‑229 (MQ=255) gCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCt > 2:529964/1‑229 (MQ=255) gCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTGTTGCt > 1:712488/1‑229 (MQ=255) ctgggcgCCGGGA‑TGATCGAAACGGGGGTTGGGATCCCGTCGATGTGCCTGTGCGCACATCGTCGGTGTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTg < 1:691169/223‑1 (MQ=255) ggTTGCCGCGA‑TGATCGGAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTg < 1:509178/227‑1 (MQ=255) cgagtggttaaaagggaccggTGTTGGGATCCCGGCGATGGGCCGTTCCGCACATCGGCGCTTTCCCTTTGGGAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGGTTTGCTGAAggg < 2:559633/210‑1 (MQ=255) atCGAAACAGGGTTTGGGTTCCGGTCGGGGGGCCCGTCCCGCCATGGGCGCTTTCCCTTTGGTAGCCGGGCCGGG‑GGCGGGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTggg < 2:188302/229‑1 (MQ=255) aaCAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGACTATTACGAAGACCACGACGAAGGCACCCTGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGtt > 1:84888/1‑228 (MQ=255) gtgtTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCgg > 1:668095/1‑191 (MQ=255) gtgtTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGCCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCCCCATCCAGCTGGCAGGCTATGACGTCTATCACGAAGACCCCGACAAAGGCACCCGGCAACGTTTTGCTTCAAGGCTgcagcggacaggcgcaca > 2:64805/1‑212 (MQ=255) gtgtTGGGATCCCGTCGATGTGCCTGTCCGCACAGCGTCGCTTCCCCATTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCgg < 2:668094/191‑1 (MQ=255) | AATCGACGCTATCTGGGGAGGTGCGCGTGCGTAATCATCAGTTAGGGATTTATGAAAAAGCGCTGGCGAAAGATCTCTCCTGGCCGGAGCGGCTGGTGCTGGCAAAAAGCTGCGGTTTTGATTTTGTCGAAATGTCGGTGGATGAAACCGACGAACGGCTCTCACGTCTTGACTGGAGCGCCGCACAAAG‑GACTTCGCTGGTTGCCGCGA‑TGATCGAAACAGGTGTTGGGATCCCGTCGATGTGCCTGTCCGCACATCGTCGCTTTCCCTTTGGTAGCCGTGACGAG‑GCAGTGCGCGAACGGGCGCGGGAAATCATGAGTAAAGCGATTCGCCTGGCGCGCG‑ATCTCGGCATTCGCACCATCCAGCTGGCAGGTTATGACGTCTATTACGAAGACCACGACGAAGGCACCCGGCAACGTTTTGCTGAAGGGCTGGCGTGGGCAGTCGAACA > NC_000913/3749205‑3749655 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |