| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 351,510 | T→C | 24.7% | L99P (CTC→CCC) | prpD → | 2‑methylcitrate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 351,510 | 0 | T | C | 24.7% | 52.5 / 2.9 | 37 | L99P (CTC→CCC) | prpD | 2‑methylcitrate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (11/14); new base C (5/5); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCAATGATCAAAGCCCATGAAATTCAGGGCTGCATCGCGCTGG‑AAAACTCCTTTAACCGCGTCGGCCTCGACCACGTTCTGTTAGT > NC_000913/351282‑351738 | tCATGAACTACGAAATCAGCTCTAAAGTGGCCTACTACCCCGCACATTACTGCCTGCCCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACGTCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCt < 1:668104/229‑1 (MQ=255) cGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATCCCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGCACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCCTTTAACATCGGCGCGACGAACCGCCGGCCCGCTTTcc > 2:479976/1‑226 (MQ=255) gCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCACCTCGACCCCGTCCAGGCGGCATTTAACACCAGCGCGATGCACCGCTGGCGCGATTTCCACAAAAccga > 2:126944/1‑227 (MQ=255) ctctAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGCCGTACCCACCGGCCGTCGCGCCCCCCGAACCCCGTTCCAGCTCGACACCGCCCCGCCGCCATTTACCATCCGGGCCATGACTCCCTGGCCCGCTTTCAACGCAACctgg > 2:580222/1‑229 (MQ=255) aaaGTGGCCTACCACACCGCCCAGTCCTGCCTCCTCGACCCCGCCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACctggctgg < 1:564466/229‑1 (MQ=255) gCCTACGACACCGCACATTACTGCCTGCTCGCCACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCACACCGACCCCGCCCAGGCGGCATTTACCATCGGCGCCCGGCTCCGCTGGCCCCGTTTCCACGAAACccgggtgggagcg > 2:662381/1‑223 (MQ=255) cGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGcgggggcggg > 1:689764/1‑224 (MQ=255) cacCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCCACGAAACCTGACTGGCGGCGGAGTggggg > 1:25347/1‑228 (MQ=255) cGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCTCGATGATCCGCTGGCTCGATTTCAACGATACCTAGCTGGCGGCGGGGTGGGGCCa > 1:473993/1‑228 (MQ=255) cGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCCCGACCCCGTCCCGCCGGCAGTTAACATCGGCCCGAGGATCCGCTGGCCGCGTTTCAACGAAACCTGCCTGCCCGGGGGGTGGGGCCac > 2:92638/1‑228 (MQ=255) acaTTACGGCCTGCTCGACCCGCTCGGCTGCGGTCTGGAAGCTCCCGAATCCCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCt < 1:548645/229‑1 (MQ=255) tcctccctgctctaccgcgtCGGCGGGGGTCCTGAAGCCCCAGAACACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGGCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCg < 2:135463/210‑1 (MQ=255) tACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCCTTTAACATCGGCCCGATGCTCCGCTGCGTCGCTTTCAACGAAACCATGCTGGCGGCGGGGGGGGGCCATCCTTCCg > 2:634159/1‑229 (MQ=255) tgcctgcTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGCTGATCAGCTGGCTCCATTTCAACGCTACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCggc > 1:57393/1‑226 (MQ=255) cgcctgcGCGACACGCTCGGCTGCGGTCCGGAAGCTCCCGAATACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGGGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACa < 1:193017/228‑1 (MQ=255) ggCCGCGGTCTGGGAGCCCTCGAATCCCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTgg < 1:115999/229‑1 (MQ=255) ctcGAATTCCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTTCCCATCGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGCCCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATGCCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCACGGCGGCATTCTGGCAACGGCGGACTGGCtt < 1:662382/228‑1 (MQ=255) aaaaaCTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGCGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCCGCCAACCCGGGCGCCa > 1:68518/1‑228 (MQ=255) ggctgGGGCCAATTGATCCAGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGGCCCCGTCCAGGCGGCATTTAACATCGGCGCGATGAGCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCa < 1:645989/227‑1 (MQ=255) tcgtCCCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCt < 1:22722/229‑1 (MQ=255) tcgtACCAAACGGCGTGCGCGTCCCCGGAACTTAGTTCCAGCTCGACCCCGTCCAGGCGGCATTGAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATGCCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCt < 1:92638/229‑1 (MQ=255) caccgcggTGCGCGCCCCCGGAACTCAGTCCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGGGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCa < 1:64896/222‑1 (MQ=255) aaCGGCGTGCGCGTCCCCGGAACGCAGTTCCAGCTCGTCCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCaa < 1:140538/229‑1 (MQ=255) cGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCCCGATTTCAACGCTACCTGGCTGGCGGCGGGGGGGGGCCATCCTTCCGACAACCTCGGCGGCTATCTGGCAGCGGCGGCGTGGCTTTCGCCCCACGCGGGCGCCACGGGCAAAGCCGCGGCGACCAGGAAACAGAGGCTGGcccacaa > 2:85219/1‑223 (MQ=255) ccGGAACTCAGTCCCAGCCCGCCCCCGTCCGGGCGGCATTTAACATCGGCGCAATGACCCGCTGGCCTGATTTCAACGATTCCCGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCAATGATCAAAGCCCATg < 1:376801/228‑1 (MQ=255) gctttaGTTCCAGCCCGACCCCGCCCGGGCGGACTTGAACCTGCGCGGGATGATCCGCTGGCTCGATTTCAACGATACCTGGCGGGCGGCGGAGTGGGGCCATCCTTCCGACAACCGCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCAATGATCAAAGCCCATGAAAtt < 2:25347/224‑1 (MQ=255) aGTTCCAGCCCGACCCCGGCCAGGCGGCAGTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATCCCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCAATGATCAAAGCCCATGAAATTCAggg < 1:479976/229‑1 (MQ=255) ttCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCCTGAAACAGGGGCTGACCGCACTGATCAAAGCCCATGAAATTCatg > 1:490516/1‑224 (MQ=255) ttCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCACTGATCAAAGCCACTGAAATTCAgg > 1:379783/1‑226 (MQ=255) cAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGCTGACCATGAAACAAGGGCGGACCGCACTGATCAACGCCACTGAAATTCAGGGCTGcc > 1:173603/1‑228 (MQ=255) gctggtccccGTCCAGGCGGCATTTAACAACGGCGCGATGAACCGCTGGCCGGAATTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCAATGATCAAAGCCCATGAAATTCAGGGCTGCAt < 1:422804/222‑1 (MQ=255) cgcccccGTCCAGGCGGCATTTAAAATCGGCGCGGTGCTCCGCTGGTCCGATTTCAACGATACCGGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGGGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCAATGATCAAAGCCCATGAAATTCAGGGCTGCATcgcg < 2:68518/226‑1 (MQ=255) ccAGGCGGTATTGAACACGGGCGAGCAGAACCGTCTGCGCGAGTTCAACGTTACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCTGTATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCAATGATCAAAGCCCATGAAATTCAGGGCTGCATCGCGCTGG‑AAAAc < 1:734693/229‑1 (MQ=255) tttttagcgcGGCGCGAGGAGCCGCCGGCCTGATGTCAACGGTGCCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACATCCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCAATGATCAAAGCCCATGAAATTCAGGGCTGCATCGCGCTGG‑AAAACTCCTTTAAc < 2:434175/220‑1 (MQ=255) cGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCCAAGCGCCGGTGACCATGAAACAGGTGCTGACCGCAATGGTCAAAGCCCATGAAATTCAGGGCTGCACCCCGCTGGAAAAACCCCTTTacaccctcggc > 2:561105/1‑219 (MQ=255) atCCGCTGGCTCGTTTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCAATGATCAAAGCCCATGAAATTCAGGGCTGCATCGCGCTGG‑AAAACTCCTTTAACCGCGTCGGCCTCGACCACg < 1:580223/229‑1 (MQ=255) cccGCTGCCCCGATTCAAACGATCCCGGGTGGGCGGCGGGGTGGGGCCGCTCTTCCGACAACCCCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGGAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCAATGATCAAAGCCCATGAAATTCAGGGCTGCATCGCGCTGG‑AAAACTCCTTTAACCGCGTCGGCCTCGACCACg < 2:627338/227‑1 (MQ=255) ccGCTGGCTCGCTTTCAACGATTCCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGTCGGACTGGCTTTCGCCCAAACCGGTCGCCAGAGGCAAAACGCCGATCACCACGAACACGGGGCTGACCGCAAGACTCAAAGCCCCTGACATTCAGGGCAGGAACCCACAGG‑AAAACCCCCTTAACCCCGTcaacacagagcacctt > 2:82353/1‑214 (MQ=255) tCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCAATGATCAAAGCCCATGAAATTCAGGGCTGCATCGCGCTGG‑AAAACTCCTTTAACCGCGTCGGCCTCGACCACGTTCTGTTAGt > 1:530228/1‑229 (MQ=255) | TCATGAACTACGAAATCAGCTCTAAAGTGGCCTACGACACCGCACATTACTGCCTGCTCGACACGCTCGGCTGCGGTCTGGAAGCTCTCGAATACCCGGCCTGTAAAAAACTGCTGGGGCCAATTGTTCCCGGCACCGTCGTACCCAACGGCGTGCGCGTCCCCGGAACTCAGTTCCAGCTCGACCCCGTCCAGGCGGCATTTAACATCGGCGCGATGATCCGCTGGCTCGATTTCAACGATACCTGGCTGGCGGCGGAGTGGGGCCATCCTTCCGACAACCTCGGCGGCATTCTGGCAACGGCGGACTGGCTTTCGCGCAACGCGGTCGCCAGCGGCAAAGCGCCGTTGACCATGAAACAGGTGCTGACCGCAATGATCAAAGCCCATGAAATTCAGGGCTGCATCGCGCTGG‑AAAACTCCTTTAACCGCGTCGGCCTCGACCACGTTCTGTTAGT > NC_000913/351282‑351738 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |