Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,366,216	T→G	23.4%	T187P (ACT→CCT)	dcuA ←	C4‑dicarboxylate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,366,216	0	T	G	23.4%	104.4 / 3.6	65	T187P (ACT→CCT)	dcuA	C4‑dicarboxylate antiporter
Reads supporting (aligned to +/- strand):  ref base T (36/11);  new base G (4/13);  total (40/25)
Fisher's exact test for biased strand distribution p-value = 2.45e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTGCGTTGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGAT  >  NC_000913/4365989‑4366432                                                                                                                                                                                                                                          |                                                                                                                                                                                                                           gATTGCGTTGGTGGGTTTCATCCGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGACTGCATAGATAACCCAGCCATCTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGt                                                                                                                                                                                                                            <  1:712863/228‑1 (MQ=255)  aTTGCGTTGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCATACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGAAGTTCAACC‑AGGCCCTC‑TT‑CCAGACCCTTCCGATAAATCGGATCGTCAGGGAGTTTGGAGTTGAAC‑AG‑AATAGGGa                                                                                                                                                                                                                          >  2:725291/1‑229 (MQ=255)    tGCGTTGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGATTTTGCACCGGAATTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATGGGATCGTAAAAGGGTTTGGAGTTGACC‑AG‑CGTAAGGAcc                                                                                                                                                                                                                        >  1:335995/1‑229 (MQ=255)        ttGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCagg                                                                                                                                                                                                                     >  1:430005/1‑228 (MQ=255)             tgtttttattagggggttttCAACCAGACCCCAGCTTGGGCTGTGTATGATTGGATAGATAACCACGCCAAATTCGCCCAGCAGGACAAGCGAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGAc                                                                                                                                                                                                               <  2:678478/213‑1 (MQ=255)              gttgtcatcaGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGAc                                                                                                                                                                                                               <  1:198571/224‑1 (MQ=255)                         cGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGGGTTTGGAGTTGAAC‑AG‑CATATTGACCAGGAAGGACAT‑CACCCGAAcc                                                                                                                                                                                                  >  1:271176/1‑229 (MQ=255)                                      aGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTgg                                                                                                                                                                                     >  1:167178/1‑229 (MQ=255)                                       ccaccctgtggggggTGGTGATGATTGAGAAGATACCCACGCCACCTCCGCCCAGCGGCAACAGCCACACGGACGTTTTTGCCCGGGTTTTGTTTTCAATCT‑GCTTTTAACCGGTCGGTGCAACC‑GGCCCCTC‑TC‑CCAGGCGCTTGCGAAAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTAACCAGGAAGTACAT‑CACAAGAACCGCCAGCAGGGTGGa                                                                                                                                                                                    <  2:703937/218‑1 (MQ=255)                                             tGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGCTTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGCCATAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑ACCCAGAACCGCCAGCCGGGTGTACGGGAt                                                                                                                                                                              >  1:330352/1‑229 (MQ=255)                                             tGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGCGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAC‑AACCAAAACCGCCAGCAGGGGGGAAGGGAt                                                                                                                                                                              >  2:626140/1‑229 (MQ=255)                                                        ttGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAAAGAGGTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGCCAT‑CACCAACACCGCCAGCAGCGGGGGCGGGAAGagacaggaaa                                                                                                                                                                   >  2:222897/1‑220 (MQ=255)                                                                  cATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGCAGGACAT‑CACCAGAACCGCCAGCAGGGTGGAAGGGATGACCACGGAGAGCAGATGGa                                                                                                                                                          >  1:735382/1‑228 (MQ=255)                                                                  cATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATggg                                                                                                                                                          >  1:167341/1‑227 (MQ=255)                                                                  cATAGACAACCACGCCAACTACGCCCAGCCGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTAACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTAGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CACAGTGACAAGGAAGGACAT‑AACAAGAACAGCGAGCCGGGGGGTCGGGATGACCACGCGGAGCAGAAGGAg                                                                                                                                                         >  2:401670/1‑229 (MQ=255)                                                                         accatccccAACTACGCCTGGCAGAACCGACCGGGGGGCCTTTTGTCCACGGGATTTGATTTCAATCT‑GCTTTTCCCCGGGCAGTGCAACC‑AGGCCTTT‑TT‑CCAGCCGCTTGCGATAAATCGGATCGCCAGAGAGTTGGGAGTTGAAC‑AG‑AATGGGGCCCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTg                                                                                                                                                  <  2:134578/222‑1 (MQ=255)                                                                            cACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGCGTTGAAC‑AG‑CATAGTGACCAGGAAGGCCAT‑CACCAGAACCGCCCGCAGGGGGGACGGGATGACCACGGGGAGCAAAAGGAGGGAGCTGag                                                                                                                                                >  2:250240/1‑227 (MQ=255)                                                                             aCGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCCAGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGACCAGATGGAGGAAGCTGGTGc                                                                                                                                              >  1:754250/1‑229 (MQ=255)                                                                               gCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAAAGCAGATGGAGGTAGCGGAGGcg                                                                                                                                             >  1:105383/1‑227 (MQ=255)                                                                               gCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGCGGACGGGATGACACCGGAGAGCAGAAGGAGGCAGCtaaggcat                                                                                                                                            >  1:161810/1‑222 (MQ=255)                                                                                   aCTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACAGCCAGCAGGGTGGACGGGAGAACCACGGTAGGCAGAGGGAGGAGACTGATGCCATGAc                                                                                                                                        >  1:735717/1‑229 (MQ=255)                                                                                    cTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGAcc                                                                                                                                       >  1:278630/1‑229 (MQ=255)                                                                                      aCGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGGGACCAGGACGGACAT‑CACCAGAACCGCCAGCAGGGGGGAAGGGATGACCCCGGAGAGCAGATGGGGGAACCCGAGGCCAtagcctc                                                                                                                                     >  2:630907/1‑223 (MQ=255)                                                                                            agcagGACCGCCCGGCCGGTCGTTTTTCCCCCGGTTTTGATTTCACTCG‑GTTTTTCACCGCGAGTTTCAACC‑AGGCTCTC‑TT‑CCAGACGCTGGCGATAAATGGGATCGTCAGAGAGTTGGGAGTTGAAC‑AG‑CATGGGGACCAGGAGGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCa                                                                                                                               <  2:413157/229‑1 (MQ=255)                                                                                             gcagGCACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACAGCCAGCAGGGTGGACGGGGTGACCACGGGAGGCAGATGGGGGCACCCGAGGCCATGACCTTcaacaac                                                                                                                              >  2:62873/1‑223 (MQ=255)                                                                                                 aaccgccctcgcggctgTTTTTGCAACGTCTTTGCTGTCAACCT‑GTTTTACCCCGCCGAGTCCAACC‑‑GGCCCTCATC‑CCAGACGCTTGACAAAAGAGGGATCGACAGAAGGTTGGGGGGTAAAC‑GT‑GAAAGTGACCATGAAGAACAC‑CACCAGAACCGACAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGaa                                                                                                                          <  2:330352/213‑1 (MQ=255)                                                                                                  aaacagcgcggcggagTTTTTGCAGCGGTTTTTGATTCAAACTGGATTTCCACCCCGAAGTTCAGCA‑CGTCCTTC‑TT‑CCAGAGGCTTGAGAAAAAGGGTACGGAAAGGGAGTTTGGGGTGCAAA‑GG‑GTTGGGGCCCAGGAAGGAAAT‑CCCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGaa                                                                                                                          <  2:31456/214‑1 (MQ=255)                                                                                                  aaCAGCCAGACGGACGTTTTGGCCCGGGATTTGATTTCAATCT‑TGTTTTCCCCGGGAAGTTCACAC‑AGGCCCTC‑TT‑CCAGACGTTTGCGATAAATGGGATCGTAAGAGAGTTGGGAGTGGAAC‑GG‑CATAGTGACAAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAg                                                                                                                         <  1:174603/229‑1 (MQ=255)                                                                                                    cAGCGAGGCGCACGTTTTTGCCCGGTTTTTTATTCCAATCT‑GCTTTTCCCCGGCAAGTTCACCC‑AGGCCCTC‑TT‑CAAGCCGATTGCGATAAATCGGATCGTCAGGGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGAAAT‑CACAAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑Ac                                                                                                                      <  1:30923/229‑1 (MQ=255)                                                                                                      gCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGACCAT‑CACCAGAACCGCCAGCAGGGTGGAGGGGATGACCACGGGAAGCGGATGGAGGTGCCTAGTTCCATGACCTTCCATGATGGacagaag                                                                                                                     >  1:379541/1‑223 (MQ=255)                                                                                                                             gatttgatttCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGTTAACTCGGATCGTCAGAGAGTTTGGAGATGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGTATAGCAGATGGAGGTAGCTGATGCCATGCCCTTCCATCACGGCAGCACTTG‑AAAACACTGCCGCTgagaacg                                                                                             >  1:133770/1‑226 (MQ=255)                                                                                                                             gatttgatttCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGCGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACAAGAACCGCCAGCAGGGGGGACGGGATGACCACGGAGAGCACATGCAGGACGCTGAGGCCATCCCCTTCCCTCACGGAAG‑CACTGAAAACCCCCGCCGCGgaatatg                                                                                             >  2:587787/1‑224 (MQ=255)                                                                                                                             gatttgatttCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGCGCAGATGGAGGTGGCTGATGCCATAACCTTCCCTCACGGGAG‑CAAGGTAAACCCCAGCCGCTCAGATTg                                                                                             >  2:79421/1‑229 (MQ=255)                                                                                                                             gatttgatttCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGAGATAAATCGGATCGTAAGAGAGGTTGGAGTTGCAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGGCCACGGAGAGAAGATGGAGGTAGCTGTGGCCAGGACCTTCCATCCCGGAAG‑ACAGTTAAACCCCTGccccggcaattg                                                                                             >  2:477591/1‑219 (MQ=255)                                                                                                                                      tCAATTT‑GCTGTCCCCCCGGCAGTAAAACC‑GTGCCCAC‑TC‑CCAGACGTGTGGGATAATGCGGACCGGAAGGTGGTTGGGAGTTGAAC‑AG‑CATAGTGCCAAGGAAGGAATG‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCgg                                                                                    <  1:401670/229‑1 (MQ=255)                                                                                                                                      tCAATCT‑GCGTTGCCTCGCGCAGTACAACC‑AGGCCCTC‑TC‑CCAAACCCTTGCGAAAAAGCGGATCGCCAGAGAGTTGGGAGTGGAAC‑AG‑CATAGGGACAAGGAAGGACAC‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCgg                                                                                    <  1:128871/229‑1 (MQ=255)                                                                                                                                       cAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAACTCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGCCAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTACTGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTTGGATTGGCGATGCGGt                                                                                   >  1:592810/1‑229 (MQ=255)                                                                                                                                        tcattcggttttcccccgcgtgTTCAACC‑CGGCCCCG‑TTGCAAGACGCTGGCGATAAATCGGATCGGCAGAAGGTTGGGAGTGGAAG‑AG‑AATGGGGACCAGGAAGGCAAC‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCgg                                                                                    <  2:688836/208‑1 (MQ=255)                                                                                                                                           tgcggttttCCCCGCGCAG‑TCAACCAAGGCCCTT‑TC‑CCAGACCTTAGCGTTAAATGGGATCTTCAGAGAGTTTGGAGTTGAAC‑AG‑CTTAGAGACGCGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCCCGGAGAGCAGATGGAGGTAGTTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGAt                                                                                <  1:630908/224‑1 (MQ=255)                                                                                                                                                  ttCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGCTAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CGCCAGAACCGCCAGCAGGGTGGACGGGATGAGCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGCAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATTCAGGGGTCGCaaa                                                                          >  1:86811/1‑227 (MQ=255)                                                                                                                                                   tCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGGTTGGAGTTGAAC‑AG‑CATAGTAACCAGGAAGGACAT‑CACCAGAACCTACAGCAGGGTGGCCGGTATGACAACGGAGAGCAGATGGAGGTAGCTGCTGCCATGACCTTCCATCACGGAAT‑ACATGTAAACCACTGCACCTGAGATAACCGATGCgcgtaccacaatca                                                                        >  2:238173/1‑216 (MQ=255)                                                                                                                                                   ccccagcgtggtcaaaCC‑GGGCCCTC‑TC‑CGAGACGTGGGCGAAAAAGCGGGTGGTAAGCGAGTTGGGAG‑TGAAC‑AGTCATGTGGACCGGGAAGGAATA‑CCACAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCt                                                                        <  2:430005/215‑1 (MQ=255)                                                                                                                                                     ccccggtaGTTAAACC‑AGGCCCTC‑CT‑CCAACCCTTTGCGATAAATCGGATAGGAGGAGAGTTGGGAGTGAAAG‑AG‑CATAGGGACCAGGAAGGAACT‑CACGAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTgc                                                                      <  2:441782/222‑1 (MQ=255)                                                                                                                                                      ccGCGGAGTCCAACG‑AGGCCCTA‑CT‑ACAGACCTGGGCAATAAAGCGGATCGCCAGAGAGTTGGGAGTTGAAC‑AG‑CATAGGGACCAGGAAGGACAC‑CCCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTgcg                                                                     <  1:280164/229‑1 (MQ=255)                                                                                                                                                       cgcgCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTg                                                                       >  1:587086/1‑226 (MQ=255)                                                                                                                                                        ggcaactccacgc‑AGCCCCTG‑TT‑CCAGACGCTTGCAATAAATCGGATCGT‑AGGGGATTTGGGGATTAACAAG‑CATAGTGACCAGGAAGGAAAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGcgcg                                                                   <  1:250240/217‑1 (MQ=255)                                                                                                                                                         cgcAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGGGTTTGGAGTTGAAC‑AG‑CATAGGGACCAGGCAGGACAT‑CACCAGAACCGCCAGCAGGGGGGACGGGATGACCACGGAGAGCAAATGGAGGCAGATGCTGCCATGCCCTTCCATCACGGAGG‑ACAGGTAAACAAGTGCCCCCGGAATTGGCTAAGCgggagtgcaaaactgcgcgg                                                                  >  2:55775/1‑211 (MQ=255)                                                                                                                                                         cgcAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGCCCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGCCGATGCGTGGATCCCAACCTTCGCgg                                                                  >  1:399678/1‑229 (MQ=255)                                                                                                                                                         cgcAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGAGGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCAATGCGCCTGAGATTGGCGTTGGGGTGAACGCAATCTACGCgg                                                                  >  2:199241/1‑229 (MQ=255)                                                                                                                                                         cgcAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACAACGGAGAGCAGATGGAGGAAGCTGATGCCAGGACCTTCCATCACGGAAG‑AAATGTAAACCACTACCGCTAAGATTGCCGATGCGGTGCTCGCAATCGGCCCgg                                                                  >  1:174281/1‑229 (MQ=255)                                                                                                                                                               tCAACG‑AGGACTTC‑GT‑CCAGTCGTTTGCAAAAACGCGGACCGTCGGAAGGTTGGGAGTTAAAC‑AG‑CAGAGGGACCAGGAAGGACAC‑CCCCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCa                                                            <  1:202525/229‑1 (MQ=255)                                                                                                                                                               caaaCC‑AGGCCCTT‑TA‑CAGGAGGTTGGCGATAATGCGGATCGACAGGGAGTTGGGATTTGAAC‑AG‑CATAGGGACAAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCa                                                            <  1:158022/227‑1 (MQ=255)                                                                                                                                                               aaaaCC‑GGCCCCTT‑TT‑CCAGCCTTGTGCGAAAAAGCGGATCGTCAGAGGGTGGGGAGTTGAAC‑AG‑CAGAGTGACCAAGCAGGACAT‑CACCAGAACCGCCAGGAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCa                                                            <  2:192452/227‑1 (MQ=255)                                                                                                                                                                ccaacggggCCCTG‑GT‑CAAGACGGGGGCGATAAAGCGGATCGGCAGAGGGTTTGGATTGAAAC‑AG‑AAAGGGAACCAGAAAGGAAAC‑AACGAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCa                                                            <  2:735381/222‑1 (MQ=255)                                                                                                                                                                  aCC‑AGGCCCTC‑TT‑CCAGCCGCTTGCGATACATCGAATCGAAAGAGAGTTTGGAGTAGAAC‑GG‑CATGGTGGCGAGGAAGGACAA‑CACTAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGATGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTg                                                         <  2:592809/229‑1 (MQ=255)                                                                                                                                                                       ggCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGAGGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGGGATTAGCGATGCGGTGATCGCAATCTGGGCGGAAACCACTGCAgg                                                     >  2:224731/1‑228 (MQ=255)                                                                                                                                                                             c‑tT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGAGACCACTGCAGTAGACAg                                               >  1:522824/1‑229 (MQ=255)                                                                                                                                                                                        gCTTGCAAAAAATCGGACGTTGAGGGGTTTTGGGGTTGACA‑AG‑CATGGTGCCCAGGAAGGCACT‑CACAGTAGCCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTTGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACggta                                      <  2:573272/229‑3 (MQ=255)                                                                                                                                                                                          ttGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGCTACCACTGCAGTAGACAGCGGACGGCa                                      >  1:91357/1‑227 (MQ=255)                                                                                                                                                                                          ttGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAAAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCCCGGAAG‑ACATGTAAACCACCGCCGCCGAGATTAGCGATGCCGTTATCGCCATCTACGCGGACACCACTGACGGAGACAacggacggaa                                      >  2:195440/1‑225 (MQ=255)                                                                                                                                                                                            gCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTCCGCGGATACCACTGCCGTAGACAGCGGACGGCAAGGt                                  >  1:337953/1‑229 (MQ=255)                                                                                                                                                                                            gCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACAACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGTTACCACTGCAGTAGACAGCGGACGGCAAGGt                                  >  1:331233/1‑229 (MQ=255)                                                                                                                                                                                               aaaaatcggcgtgctgaggggTTGGGAGTTAAAC‑AG‑CATGTGGCCCAGAAAGGACAA‑CACCAGACCCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTa                               <  1:60975/209‑1 (MQ=255)                                                                                                                                                                                                               ggagtttgggatgggAAC‑AG‑CAGAGGAACCAGGAA‑GACATGCACCAGAGCCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCg               <  1:626141/215‑1 (MQ=255)                                                                                                                                                                                                               gagagTTTGGCGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGAAGACAGCGGACGGCAAGGTTTAACGCATTGTTCCTTCg               >  1:486312/1‑229 (MQ=255)                                                                                                                                                                                                                            tGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCCCGGATACCACTGCAGTAGACAGCGGACGGCAGGGTTTAACGCCTTGGTCCTTCCCCACCTCAGCGAt  >  2:77364/1‑229 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                           GATTGCGTTGGTGGTGTTCATCAGCGGTTTTTCAACCAGACCCATGCTTGGGCTGTTGATGATTGCATAGATAACCACGCCAACTACGCCCAGCAGGAACAGCCAGACGGACGTTTTTGCACCGGATTTGATTTCAATCT‑GCTTTTCACCGCGCAGTTCAACC‑AGGCCCTC‑TT‑CCAGACGCTTGCGATAAATCGGATCGTCAGAGAGTTTGGAGTTGAAC‑AG‑CATAGTGACCAGGAAGGACAT‑CACCAGAACCGCCAGCAGGGTGGACGGGATGACCACGGAGAGCAGATGGAGGTAGCTGATGCCATGACCTTCCATCACGGAAG‑ACATGTAAACCACTGCCGCTGAGATTGGCGATGCGGTGATCGCAATCTGCGCGGATACCACTGCAGTAGACAGCGGACGGCAAGGTTTAACGCCTTGTTCCTTCGCAACTTCAGCGAT  >  NC_000913/4365989‑4366432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑