| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,464,860 | A→C | 14.5% | V441G (GTG→GGG) | treB ← | trehalose‑specific PTS enzyme: IIB and IIC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,464,860 | 0 | A | C | 14.5% | 156.4 / 3.8 | 83 | V441G (GTG→GGG) | treB | trehalose‑specific PTS enzyme: IIB and IIC component |
| Reads supporting (aligned to +/- strand): ref base A (45/18); new base C (2/17); total (47/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.60e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGC > NC_000913/4464653‑4465076 | tctTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCCCGGTTGaa > 1:78375/1‑229 (MQ=255)tctTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAACCCAAAACCCTGCCAGTAGCTCGGttta > 2:549551/1‑226 (MQ=255)tctTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACAAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGTCAGGTTGaa > 1:483484/1‑229 (MQ=255) tttGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAAt > 1:216530/1‑228 (MQ=255) tttGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGCGAGTACAATCGGGATGATGATGGCGATAGCCCTTGCCAGCGCAAACCCCTGCCAGTACCCTGGTTGAAt > 1:137394/1‑228 (MQ=255) tttGGATAAATCTGGGAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAAt < 1:497548/228‑1 (MQ=255) ttaaTTTGGTAAATAACCGTGTTTTGCCACCAGGGGGGAAATAGGTTCATTACGTTAATCCTGCGAATGCGAGGGGGCGCAATTGCGACCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCgagaga < 2:300438/227‑1 (MQ=255) gTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTCCCAGTAGCCCGGTTGAATCGAGAGAATTCCCgg > 1:74197/1‑229 (MQ=255) gTAGATAACGCCGTTTTGCCACCAGTGAGGGAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGCGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGACG‑GTATTTCCGCCGATAGATAAACGAGGGGAGTACAATCGGGATGATGATGGCGATAGCCATTGCAATGGCAAAAACCTGCCAGTATCCCGGTTGAATCggaaaaatttccag > 2:236352/1‑216 (MQ=255) gTAGATAACGCCGTTTTGCCACCAGAGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGCGTACAATCGGGATGACCATGGCGATAGCCATTGCCAGCGCAAAAACCTTCCAGACGCTCGGTGGCATCGAGAGAGTTCCCgg > 2:389055/1‑229 (MQ=255) aaCGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGCGAATTCCAggacggcg > 1:158133/1‑223 (MQ=255) aCGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGgcgg > 1:534381/1‑227 (MQ=255) ccGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGCAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGGTAAAAGAGGTGAGGACAATCGGGATGATGAGTGCGATAGCCATTGCCAGCGCAACAACCTCCCAGTAGCTCGGTTGAACCGCGGAAGATGCCGGCAGgccgcc > 2:274527/1‑228 (MQ=255) gCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGa < 1:27963/229‑1 (MQ=255) ccaGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATTGAGAGAATTCCCGGCCGGCCGCCTCAGCCGAGGcc > 1:361870/1‑229 (MQ=255) caGGGGGGAAGGAGGGTCATTAGGTTATTCAGGGGAATGGCGGGGGGGGCAATTGGCCCCCGAAGAAAATTAACAAATGTCCAGTGGTCCCAGGGG‑GTATTTCCGCTGAAAGATAAACGAGGTGAGTACAATCGGGATGATTATGGCGATAGCCATTGCCAGCGCAAACCCCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCa < 2:261300/229‑1 (MQ=255) tGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGACGCTCGGGTGGATCGAGGAAGTTACCGGAAGGCCGCCTACGCCGCTGCCATTc > 2:512407/1‑229 (MQ=255) gggggAAGATGGATCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCTGGCG‑GGATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCAtt < 2:216530/227‑1 (MQ=255) gATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCCCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGcccaac > 1:469822/1‑225 (MQ=255) gATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCGTCCAGCAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTCCCCCCATGCCATTCCCCaaaa > 2:724137/1‑229 (MQ=255) cTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGACAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTCCAATCGGGATGTTGATGGCGATAGCCATTGCCAGCGTAAACACCTGCCAGTAGGTCGGTTGAATGGAGCAAATTCCAGGTAAGCCCCCCAATCAGCAGGCATTCGCCATAacgacggctagccccgcaaac > 2:74060/1‑214 (MQ=255) gCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGCTGCTGATGGCGATAGCCATTTCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGTGAGAATTCCCGGCAGGCCGCCTCCGCCCATGCAATTCGCCATCACCCCGTTCAGGCCGCaaacca > 2:720811/1‑223 (MQ=255) gCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATCGCa > 1:19665/1‑228 (MQ=255) gCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCCCATAGCa > 1:349267/1‑228 (MQ=255) ggAATGCGGGGGGCGGAAATTGTCCCCCGAAAAAAATTAAACAAGGGCCAGCGGGCCCAGGCG‑TTATTTCCGTTGAATGATAAACGAGGTGAGTAAAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAAt < 2:282922/228‑1 (MQ=255) cGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGGATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAAAGCCGTTCAcgccgccagccaa > 2:643889/1‑221 (MQ=255) ggggccaatttGCGCCCGTAAAAAAATTAAACAATGGCCAGCGT‑CCCCGGCGAGTATTTCCGCTGATAGATAAACGGGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCCAAAACCCCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGccagac < 2:60810/220‑1 (MQ=255) ggggCGAATTTGGCCCCGGAAGAAAATTAAACAAGGTCCAGGGTGCCCGGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTCCAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGccagac < 2:534381/229‑1 (MQ=255) ggggCCAAATTGCGCCCCGAAGAAAATTAAACAATGCCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGccagac < 1:17500/229‑1 (MQ=255) ggCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGccagcac > 1:290924/1‑225 (MQ=255) cAATTGGGCGCCATAAAAAATTAAAACATGTCGAGCGTGCCCAGGAG‑AGATTACCGCTGATAGACAAACCAGTTGAGTCCAATCGGGATGATGATGGCGGTAGCAGTTGCACGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGTCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCCTAGCAAACCTGccagagcaga < 2:728211/227‑1 (MQ=255) aaTTGCGCCCCAAAGAAAATAAAACAAAGTCCAAGATGCCCGGGCG‑GTATTTCCGGTGAAAGAAAAACGAGGTGAGTACAATCGGGATGAGGATGGCGATAGCCATGGCCAGCGCAAACCCCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAAc < 2:557312/228‑1 (MQ=255) ccccGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGGTGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTAAGGCCGCATAGCAATCCTGCCAGCCACGAACCcatcat > 1:310705/1‑228 (MQ=255) agaagaaAATTAAACAATGGCCAGGGTGCCCAGGCG‑GTATTTCCGCTGAAAGATAAACCAGTTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATcgcg < 1:512407/228‑1 (MQ=255) agaaaatTTAAAAAATGTCCAGCGTGACCGGGGG‑GTATTTCCGCGGAGAGATAAACGAGGGGAGTCCAATCGGGTTGAGGAGGGCTATAGCCATGGCCGGCGCAACCACCTGCCAGTAGCTCGGTTGAAGCGAGAGAATGCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGca < 2:158133/226‑1 (MQ=255) aagaaAATTAACAACTGTCCGGCGTGCCCAGGGG‑TTATTTCCGCTAATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCCGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGca < 2:25805/229‑1 (MQ=255) aagaaAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCGGACCCGAACCacacaccccgcc > 1:97327/1‑218 (MQ=255) aagaaAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCCGACAAGAACCAATCATCCCGca > 1:329354/1‑229 (MQ=255) aagaaAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGAAAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCCTTGCCAGCGCAAACACCTGCCAGTACCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACCCCGCTGCCATTCGCCATAAATCCAGTCCGGCCGCATAGCAACCCTGCCAGACCCGAACCAAACATCGCCca > 2:670645/1‑229 (MQ=255) aaaaaaaTTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCGGCTGATTGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACTTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGca < 1:447202/226‑1 (MQ=255) aaaaaaaTTAAAAAAGGGCCGGTGTGCCGGGGCG‑TGATTGCCGCTGATAGAGAAACGGGGTGGGTACATCCGGGATGAGGATGGCAATAGCCATTGCCAGCGCAAACTCCTGCCAGTAGCTCGGTTGAATCGAGAGAAGTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGca < 2:121510/226‑1 (MQ=255) gataaTTAAAGAGTGTACAGCGTGGCCGGGGG‑GTATTCCCGCTAGTAGATAAACGGGGTGAGTACAATCGGGATGTTGATGGCGATGGCCTGGGCCGGCGAAACCCCCTGCCAGTAGCTCGGTTGCATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAAGCCTATCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGcaca < 2:137394/226‑1 (MQ=255) atttAAACAAGTTCCAGTGTGCCCGGGCG‑GTTTTTCCGCTGATAGAAAAACGAGGTGAGTACAATCGGGATGATGATGGGGATGGCCATTGCCAGCGCAAACCCCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCa < 2:662707/227‑1 (MQ=255) tgcccAGGGGGCCCGGGCG‑GTATTTCCGCTGATAGATAAACGGGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCg < 1:600950/226‑1 (MQ=255) ggTCCAGGGTGCCCGGGGG‑GTTTTTCCGCTGAAAGATAAACGGGGTGAGTACAATGGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACCCCTGCCAGTAGCCCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCg < 2:183914/228‑1 (MQ=255) tCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCGGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGAGTCCATTACCCATAAGTCCGTTCAGGCCGCCTAGCAATCCTGCCCACCCCGAACCAACCCTCCCCGACAGCCTCGGGaaaggat > 2:595692/1‑224 (MQ=255) tCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCCTGCGCCATAAGGCCGTCACGGCCGCATGGCAATCCGTCACGACCAGAACCCATCAACccgccccaccacggggacgcat > 2:479272/1‑211 (MQ=255) cGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTAAGGCCGCCTAGCAATCCCGCCAGACCAGAAACAATCATCGCGCACAGCCACGGGAACGGAGAttt > 2:450961/1‑228 (MQ=255) cccAGGGG‑GTATTTCCGCTGAAAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGGAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCg < 1:311759/218‑1 (MQ=255) cccAGGCG‑GTATTTCCGGTGATAGAAAAACGAGGTTAGTACAAGCGGGATTATGATGGCGATAGCCAGTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGt < 1:643890/229‑1 (MQ=255) cccAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGACTCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACGCCATCGGGAAGCGATATTTCAgg > 1:411410/1‑228 (MQ=255) cccAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGAGAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCCCCATAACGCCGTTCAGGCCGCATAGCCATCCCGCCAGACCAGACCCACACACCGCGCACAGCACCCGGAAACGACATTTccagg > 2:311759/1‑225 (MQ=255) ccaggggg‑gTTTTTCCGCGAATAGATAAAAGGGGTGAGGAAAATCGGGATGGAGATGGCGATAGCCATTGCCAGGGAAAACCCCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGt < 2:427754/222‑1 (MQ=255) ccAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAACGGAGATTTCCGGtt > 1:223758/1‑229 (MQ=255) gggatttttCCGTTTGTAGATAAACGGGGTGGATCCAATCGGTATGATGTTGGCGTAAGCCTGTGCCAGGGCAAGCCCCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCAGTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATg < 2:535325/223‑1 (MQ=255) gctgTATGTCCGCAGAAAGATAAACGAGGTGAGTACAACCGGGATTATTATGGCGATAGCCAGTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCCTCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATg < 1:687205/226‑1 (MQ=255) tttCCGGTGTTAGATAAACGGGGTGATGCCAATCGGGTGGATGGTGGCGATAGCCATTCACCGCGCAAACCCCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTAc < 2:349931/229‑1 (MQ=255) tttCCACTGATAGGTAAACGAGGTTAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACCCCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTa < 1:546874/228‑1 (MQ=255) gatagataAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCCTAGCAATCCTGCCAGACCAGAACCAACCATCGCGCACAGCCTCGGGAAACGATACTTCAGGATGATGCCGTACATTGAAg > 2:464105/1‑228 (MQ=255) gatagataAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAg > 1:391799/1‑228 (MQ=255) gatagataAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCCCGACCAGCATCGGGAAGCGATATTTCAGGTTGATGCCGAACATTGCAg > 2:200407/1‑228 (MQ=255) gatagataAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATCCCATTCGCCCTAACGCCGTTCAAGCCGCCTAGCAATCCTGCCCGACGAGACCCAAACATCGCGCACCGCAACGGGCACCGAATTTTCAGGTTGAtacgcgaccatgaag > 2:135555/1‑214 (MQ=255) aaagagaAACGAGGGGAATACAACCGGGATGATGATGGCGATAGCAATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGGCCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAgg < 1:74060/226‑1 (MQ=255) aGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATC‑GGTAGCGATATTTCAGGTTGCGACCGTACATTGCAGGCTCAGTGACACCAAGCCa > 1:284394/1‑229 (MQ=255) tACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCCTAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGACTCCGGAAGCGATATTTCAGGGTGCTGCCGCACATTGCCGGCCCAGTGCACCCAAGcccg > 2:645430/1‑227 (MQ=255) tACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGGAGCTAGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCAATTCGCCGTACAGCCGTTCAGCCCGCCAAGCCAACCGCCCAGACCAGAACCAATCATCTGTCACAGCACCGGGGAGCGATATTTCAGTTTGTTGCCGAACCTTGCGGGCCCAAGGCCCCCAGACCAg > 2:598999/1‑229 (MQ=255) aaaagcGGGGTGAGGAGGGCGAAGACCAGTGCCCGCGCAACCCCCGGCCAGTAGCTCGGTTGAATCGAGGGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAgg < 2:78375/224‑1 (MQ=255) tCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCCTAGCAATCCTGCCAGCCCAAAACCAATCATCGAGCACAGCACCGGGCAGCGAAATTTCAGGTTTATGCCGTACATCACAGGCTACGGGCCCCCAAAcccgcggga > 2:589943/1‑222 (MQ=255) tCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCCCAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCACAGCCAGGCGga > 1:225735/1‑229 (MQ=255) tCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTCGCAGACCCGAACCACTCATCGGCCACACACTCGGGCAACACTATTTACGGTGGTACCCGGACATTACAGGCCCGGGGACCCCCAACCAGggggg > 2:735570/1‑228 (MQ=255) tCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACACCGATCCCATTCGCCATAACGCCGTTCAGGCCGCGTAGCAATCCTGCCAGACCAGAACCAACCATCGCGCCCACACCCGGGTAGCGAACTTTCAGGTTGATGCCGGACATTGAAGACCCCGTGACCCCAGGcacgggggg > 2:225765/1‑221 (MQ=255) ggATGATGATGGCGATAGCCATTGCCAGCGCAGACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGAGgaga > 1:31752/1‑228 (MQ=255) tgtgggCGATAGCCATTGCCCGCCAACACCCCTGCCGGGAGCTCGGTTGAAGCGAGAGAATCCCCGGCAGGCCTCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCggc < 2:310705/225‑1 (MQ=255) tgtggcggaaggCCTTTGCCATCGCAAACACCTGCCAGTTGTCCGGTTGAACTGAGAGAATTCCCGGCCGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCggc < 2:168618/218‑1 (MQ=255) ggCGGTGGCCATGTCCAGGGGAAACCCCTGGCAGTATCTCGGTTGAATCGAAAGAATTCCCGGAAGGCCGCATCCGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCggcagg < 2:349267/228‑1 (MQ=255) gATGGCCATTGCCAGCGCAAACCCCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACg < 1:531365/229‑1 (MQ=255) aGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGCCCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCCGGTTGATGCCGTACATTGAAGGACCATGGACCCCAAGCCAGGCGGGGATAGCGGAAAGCAcagg > 1:208793/1‑225 (MQ=255) cgtggCCAGGCCAAACCCCGGCCAGTAAGCCGGGTGAATCGAGAGAATTCCGGGCAGGCCGGCCACGGCGATGCCATTCGACATAACGCCGTTCAGGTCGCATAGCAATCCGGCCAGAACAGAACCAATCATCGCGGACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGTCCCCAAGCCAGGCGGAGATAGCGGCAGGCACggctat < 2:603916/224‑5 (MQ=255) atctcccgccaaaaCCCCTTCCAGTCGCTGGGTTGAATCGAGACAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTTAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGAtct < 2:329354/218‑1 (MQ=255) tGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCCCGCACAGCATCGGGAAGCGATATTTCAGGGTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCCGAGATAGCGGCAGGCACCgaaaacacg > 2:656306/1‑222 (MQ=255) gCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGAACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGGTAGCGGCCGGGACgggagacacgc > 2:531365/1‑220 (MQ=255) cgcAAACCCCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGca < 2:26209/129‑1 (MQ=255) cgcAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGca > 1:26209/1‑129 (MQ=255) | TCTTTGGATAAATCTGGTAGATAACGCCGTTTTGCCACCAGTGGGGAAGATGAGTCATTACGTTATTCCTGCGAATGCGAGGGGGCGCAATTGCGCCCCGAAGAAAATTAAACAATGTCCAGCGTGCCCAGGCG‑GTATTTCCGCTGATAGATAAACGAGGTGAGTACAATCGGGATGATGATGGCGATAGCCATTGCCAGCGCAAACACCTGCCAGTAGCTCGGTTGAATCGAGAGAATTCCCGGCAGGCCGCCTACGCCGATGCCATTCGCCATAACGCCGTTCAGGCCGCATAGCAATCCTGCCAGACCAGAACCAATCATCGCGCACAGCATCGGGAAGCGATATTTCAGGTTGATGCCGTACATTGCAGGCTCAGTGACCCCAAGCCAGGCGGAGATAGCGGCAGGCACGGAGATCTCGC > NC_000913/4464653‑4465076 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |