| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,471,964 | T→G | 17.9% | T145P (ACT→CCT) | pyrB ← | aspartate carbamoyltransferase, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,471,964 | 0 | T | G | 17.9% | 177.9 / 2.8 | 83 | T145P (ACT→CCT) | pyrB | aspartate carbamoyltransferase, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base T (32/33); new base G (12/6); total (44/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACTTCTTCAATAGAGCTGTGCAGACTCCATGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGC > NC_000913/4471740‑4472192 | cACTTCTTCAATAGAGCTGTGCAGACTCCATGCGCTCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGGGCGGCCAAATTTCAGGCCACCAACCATTGGGACGTGG‑GAGTGTCCAGAACCCCCCGGGGTTCCCGGAAAAGGGaaa > 2:709293/1‑228 (MQ=255) atatgggttgtgCAACCCCCATGCCATCCCTTTTTCATCGAGCATAGCCAGAATGGATTGCGGCTTTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTGCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCACCTGGGTTTCCTGAATAGTGAATAAGTCCAGc < 2:412575/221‑1 (MQ=255) tAGAGCTGTGCAGACTCCATGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGACGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCCCCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATTGGGAAAAAGTCCAGCaa > 2:506563/1‑229 (MQ=255) tAGAGCTGTGCAGACTCCATGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTACCGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGGGATTGTCCCG‑ACGCCCCCGGGTTTCCGGAATAGTGAATAAATCCAGCaa > 2:272830/1‑229 (MQ=255) tAGAGCTGTGCAGACTCCATGCGATCCCTTTTGCATCGAGCATATCCAGAATGGATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGc < 1:272830/227‑1 (MQ=255) cTGTGCAGACTCCATGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATATGGAATAAGTCCAGCAAGGttt > 1:375657/1‑229 (MQ=255) cTGTGCAGACTCCATGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGGAAATAAGCCCAGCAAGGttt > 1:384206/1‑229 (MQ=255) cTGTGCAGACTCCATGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGGGCGGCCATATTTCAGGTCACCACCCATTGCGCCGTGGGGATTGTCCAG‑ACGCCCCTGGGGTTCCTGAATAGGGAATAAGTCCAGAAAgtgtt > 2:608796/1‑225 (MQ=255) cTGTGCAGACTCCATGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGAGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACTATTGCGACGTGGGGCTTGTCCGA‑ATGCCCCTGGGGTTCCTGAATATGGAATAAGTCCAGCAAGGatt > 1:432505/1‑229 (MQ=255) tgCAGACTCCATGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCg > 2:78004/1‑229 (MQ=255) tgCAGACTCCATGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTCCCTGAATAGGGATTAAGTCAAGCAAGGTTTGct > 1:458015/1‑228 (MQ=255) tgCAGACTCCATGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGCCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGAAAGGTTTGCg > 1:520069/1‑229 (MQ=255) ccAGACTCCATGTGATCCCTTGTTCATCGAGCACATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGt < 1:333770/228‑1 (MQ=255) aCTCCATGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGGGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAAAAGTGAATAAGTCCAGAAGAGTTTGGGCCggg > 2:177937/1‑228 (MQ=255) cTCCATGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGGCCGGCGCGGTGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGGCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCgg < 1:550402/227‑1 (MQ=255) cTCCAAGCGATCCCTTTTACATCGGGCCTATCCAGAATGTATTGCGGCATTTCCAGCGGATCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGGGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCgg < 1:2614/227‑1 (MQ=255) aTGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATgtt > 2:88268/1‑228 (MQ=255) aTCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCCTATTTCAGTTCACCACCCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCAAGCACGGTTTGCGCGGGATGtgggttgg > 2:159592/1‑229 (MQ=255) tCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATgttggtt < 1:88268/226‑1 (MQ=255) cccTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAAATTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGGGACTAAGTACAGCCAGGTTTTCGTCGGTTTTTGCTTGGAg > 1:145175/1‑229 (MQ=255) cTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGGCCAG‑ACGCCCCTGGGTTTCCTGAAAATTGAATAAGTCCAGCCAGGTTTGCGCCGGATGTTGGTTGGAGcc > 2:651502/1‑229 (MQ=255) ttttCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGCGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGACGTTGGTTGGAGCCa > 1:39015/1‑228 (MQ=255) ttttCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCAt < 1:75479/229‑1 (MQ=255) tCACCGGGCGTATCCAGGAGGTATTGCGGCTTTGCCCGCGCGGCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTGCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCAc < 2:618033/229‑1 (MQ=255) aTCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGGGACTAAGTCCGGCAAGGTTTGCGTCGGATGATGGTTGGAGCCATcccc > 1:738466/1‑228 (MQ=255) cGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGGGACCGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCACGGTTTGCGGCGGAGGTTGGTTGGGGCCAACACCGGc > 2:197962/1‑229 (MQ=255) gggacatgcccgAATTTTTTGCGGCATTTCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGGTGCCGGCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTCCAGGTCCCCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCTCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCa < 2:317076/218‑1 (MQ=255) gagaATATCCAGAAAGGTTTGCGGCCTTGCCAGCGCGGCCGGCGGGGGGAAGGAAAAACGGTTGCCGGCGAACTTCGCTAACGCCTGGGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCa < 2:443701/229‑1 (MQ=255) gCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGGCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCAtt < 1:506563/229‑1 (MQ=255) aaTGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGGGGAGATTGTCCAA‑ACGCCCCTGGGTTTCCTGATTAGTGAATAAGTCCAGCAAGGTTTGCGTCGGGTGTTGGTTGGAGCCATACCCggaaatgtaccgggcc > 2:175494/1‑215 (MQ=255) ttgtttGCGGGAATGCCAGCCCGTCCGGCGCGATGGAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTa < 1:389932/224‑1 (MQ=255) tATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGACAAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGACATCACCGGCCTTCCGTACCggacca > 1:80749/1‑224 (MQ=255) aTTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCCGGTCACCAACCATTGCGACGTGGAGATTGTCCCG‑ACGCCCCCGGGATTCCTGAATCGGGAATAAGTCCCGCAAGGCTTGCGTCTGCTGGTGGTTGGGGCCATCACCGGCATTCAGTACCGGTACaat > 2:379145/1‑227 (MQ=255) aTTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGGTTCCTGAATAGGGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCCGGACCGGTACAt > 1:15183/1‑228 (MQ=255) aTTGCGGCATTGCCAGCGCGTCCGGCGCGAAGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGACTTCAGGACCGGTACAt > 1:266728/1‑228 (MQ=255) aTTGCGGCATTGCCAGCGCGTCCGGCGAGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCCGGGTTTCCGGAATAGTGAATACGGCCAGACAGGTTGTCGTCGGAAGTTGGTTGGAGCCATACCCGGCATTCAAGACCgggcaat > 2:107670/1‑223 (MQ=255) ttGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCAAT‑‑GTACCGGTActgaatg < 1:175494/229‑7 (MQ=255) ttGCCATCACGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTAAACGGTGCGGCCATATTTCAGGTCACCAACCATTACGACGAGAAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGGGAATAAGTCCAGCAAGGTATGCGCCGGATGTGGGTTGGAGCCATCCCCGGCATTCAGGTCCGGGCCATTgagcgaaaa > 2:574066/1‑221 (MQ=255) cgATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGgcgcg < 1:552038/228‑1 (MQ=255) cgATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGgcgcg < 1:514298/228‑1 (MQ=255) cgATGAAGTAAAAACGGTTGCCGTCCAACTTCACTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATTTTTCAGGTCCCCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGgcgcg < 1:608797/228‑1 (MQ=255) aaGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCa < 1:78004/228‑1 (MQ=255) aaaacCGGTTTCCGGCCAACTTCCCTAACGCCTGGGGCAGGGAGTGAACGGTGGGGCCATTTTTCAGGGCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTc < 2:375657/229‑1 (MQ=255) ggTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATACTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCTTGCg > 1:342870/1‑228 (MQ=255) gttgcgcgcGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCACTATTGCAGGTCCCCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGGCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGa < 2:534022/221‑1 (MQ=255) ggtGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGACCGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCTCCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGa < 1:160790/227‑1 (MQ=255) ctGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACATGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTTGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGGGGCCAGGCGCGCCGCACCTTCCTGCgg > 1:107615/2‑227 (MQ=255) tccGTCGTACTTCGCTAACGCTCGAGTGAGGGAGTGAACGTTGCGGCCATATTTCAGGTCACCAGCCATTGCGACGTGGAGATTGTCCAG‑ACGCCCGTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGa < 2:266728/228‑1 (MQ=255) gCCGTCCAACTTCGATAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGAt < 1:177937/227‑1 (MQ=255) cGAACTTCCCTAACGCCTGAGTCAGGGGGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCAt < 1:159592/229‑1 (MQ=255) cTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCAc < 1:197962/228‑1 (MQ=255) ccTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGGACCGGGACATTGCCGGAAAACTCGGGGGCCAGGCGCGCCGCCCCTTCCTGCGGAGGACGCAACACTATGCCGTCGAc > 2:629799/1‑229 (MQ=255) ctgggccAGGGGGTGACCGTTGGGGCCAGTTTTCGGGTCACCAACATGTGGGACGT‑GTGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCCCTATCGCATCGACGt < 1:709294/223‑1 (MQ=255) aGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGAt < 1:552208/229‑1 (MQ=255) tGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGTATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGCCgaacgggcggaaaa > 2:220773/1‑216 (MQ=255) aaCGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTCGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGACAACTCGTTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGCGGCAAGTGCTGATacc > 1:77934/1‑226 (MQ=255) gTGCGGCCATATTTCATGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTGGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTaa < 1:603754/213‑1 (MQ=255) gTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACCTTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGAAGCCTCACTATCGCAGCGCGGTAAGTGCCGATaaaccgtc > 2:603753/1‑223 (MQ=255) gTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGGGCCTCCCCTTCCTGCGGATGTCGCATCACTATCGCACCGACGAAAATGCTGCTACACGaa > 2:268310/1‑228 (MQ=255) ttcGGCCCAATTTCAGGTCACCAACCATTGCGACGTGGAGATTGGCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTa < 2:467391/136‑1 (MQ=255) tGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTa > 1:467391/1‑138 (MQ=255) tttCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGCTAACCGAAATGGTATAGGc > 1:101075/1‑228 (MQ=255) ccaaccaTTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGGATCGGCCAGCGTTTCg > 1:381992/1‑228 (MQ=255) tGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCCCGATCGCATCGACGTAAGTGCTGATAACCGAAATGGCATCGGCCAGCGTTTCGCCCtt > 1:431871/1‑225 (MQ=255) tGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCACCGCCGTAAGGGCTGTTAACCCAAATGGTATCGCCCCACGGTTCGCCCTTTTTa > 2:378339/1‑229 (MQ=255) cGAGGTGGGGGTTGTCCGG‑ACCGCCCTGGGTTTCCTGAAAAGGGAATAAGTCCAGCAAGGTTTGCGTGGGATGTTGGGTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTAcc < 2:342870/229‑1 (MQ=255) cGACGGTGAGATTGTCCAG‑GCGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCCAGGTTTGCGTCGGATGTTGGTTGGAGCCAGCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTAcc < 1:651503/229‑1 (MQ=255) ggattgggtgagtttccggtcGCCCCTGGTTCTCCTGAATGGGCAATAAGTCCGGCTAGGGTTGCGTCGTATGTTGGTTGGCGCCATCACCGGCATTCAGTACCGGTACATTGCCTGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTAcc < 2:107615/209‑1 (MQ=255) cttGGAGATTTTCCGG‑ACGCCCCGGGGTTTCCGGAATTGGCAATAAGGCCAGGAAGGTGTGCGTCGGATGTTGGTTGGGGCCATCGCCGGCATTCAGGTCCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCCCCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAg < 2:15183/227‑1 (MQ=255) ggtgggaatttccaaggcGCCCCTGGGTTTCCTGAATGGGGAATAAGGCCAGCAAGGTTTGCGGCGGGTGTGGTTTGGAGCCATCCCCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAg < 2:520069/212‑1 (MQ=255) tGGAGATTGTCATG‑ACGGCCCTGGTTTTCCGGAATAGTTAATAAGGCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCCCCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTg < 1:357143/229‑1 (MQ=255) cctgggcgcccgtggTTTTCGGAAAAGTGAATAAGGCCGGAAAGTTTTGGGTCGGATGTTGGTTGGGGCCATCCCCGGCATCCCGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGgc < 2:381992/216‑1 (MQ=255) g‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCCCCGGCATTCAGTACCGGTACCTTGCCGGAAAACTCGGTGGCCAGGCCCGCCGCACCTTCCTGCGGATGACGCAACACCTTCGCATCGCAGGACGTCAGGTCAACGGAAATGGAATCGGCCACCGTTTCGCCCTTTTTACCCCGAGACGAATTgccacg > 2:2861/1‑224 (MQ=255) ggctccctTGGGTTTCCTGAATGTGGAACAAGGTCAGCAATGTTTGAGTCGTGTGTTGGGTGGAGCCATCACGGGAATTCAGTGCGCGTACATTGCCGAAAAACTCGGTGGCCAGGCGGGCCGCACCTTCCTGCGGATGACGCATCACTATCGCGTCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTGCGCCCTTGTTACCCAGTGATGTATTGgcgc < 2:376229/224‑1 (MQ=255) gtcccTGGGTTTCCTGAATAGGATAGAAGTCCAGCAAGGGTTGTCTGGGATTGTTGTTGGAGCCATCACCGGCATTCAGTACCGGGACATGGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTTATGTATTGGCGCTGt < 2:513007/226‑1 (MQ=255) ccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGCGCTGATAACCCAAATGGTTTCGGCCAACCTTTCGCCCTTTTTACCCCGTGATGTATTgggcccttggga > 2:339350/1‑219 (MQ=255) tttCCGGAAGAGTAAATAAGTCCAGCAAGGCTGGCGTCGGATGTGAGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACACGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGcc < 1:508247/229‑1 (MQ=255) ccTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTTCCGGGGAAGCcca > 1:43705/1‑228 (MQ=255) ccTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGAAGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGAATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGAGGTATTGGCGCTGTCGGGAAAACcca > 2:193836/1‑228 (MQ=255) ccTGAATAGTGAATAAGGCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCGAGCGTTTGGCCCTTTTTACCCAGTGATGCATTGGCGCTGTCGGGGAAGCcca > 1:688755/1‑228 (MQ=255) cTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCccac > 2:373412/1‑228 (MQ=255) cTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGAATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGccccc > 1:25255/1‑226 (MQ=255) aTAGTTAATTAGTCCCGCAAGGTTGGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGc < 1:378339/229‑1 (MQ=255) tGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGCCGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAATCCCACCACGCTGGc > 1:619362/1‑229 (MQ=255) tGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGTCGCTGTCGGAGAAGCCCCCACCGCTGGc > 1:55335/1‑229 (MQ=255) | CACTTCTTCAATAGAGCTGTGCAGACTCCATGCGATCCCTTTTTCATCGAGCATATCCAGAATGTATTGCGGCATTGCCAGCGCGTCCGGCGCGATGAAGTAAAAACGGTTGCCGTCGAACTTCGCTAACGCCTGAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAG‑ACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTCGCCCTTTTTACCCAGTGATGTATTGGCGCTGTCGGAGAAGCCCACCACGCTGGC > NC_000913/4471740‑4472192 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |