Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,110,523 T→C 7.0% E166G (GAA→GGA)  rfbA ← glucose‑1‑phosphate thymidylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,110,5230TC 7.0% 183.2 / 4.1 57E166G (GAA→GGA) rfbAglucose‑1‑phosphate thymidylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (25/28);  new base C (2/2);  total (27/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

ACGAAATGCAATCTCTTCAGGACAGGAAACCTTCAATCCCTGGCGCTCTTCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATAAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCT  >  NC_000913/2110240‑2110806
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                           
aCGAAATGCAATCTCTTCAGGACAGGAAACCTTCAATCCCTGGCGCTCTTCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAAc                                                                                                                                                                                                                                                                                        <  2:41269/289‑1 (MQ=255)
     aTGCAATCTCTTCAGGACAGGAAACCTTCAATCCCTGGCGCTCTTCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGttt                                                                                                                                                                                                                                                                                   <  2:793248/289‑1 (MQ=255)
      tGCAATCTCTTCAGGACAGGAAACCTTCAATCCCTGGCGCTCTTCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGtttt                                                                                                                                                                                                                                                                                  >  2:70677/1‑289 (MQ=255)
               ttCAGGACAGGAAACCTTCAATCCCTGGCGCTCTTCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGATTCACGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGCTCTACCGGTTTTTCTTCCAg                                                                                                                                                                                                                                                                          >  2:301815/1‑288 (MQ=255)
                         gaAACCTTCAATCCCTGGCGCTCTTCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGcca                                                                                                                                                                                                                                                               >  1:619574/1‑288 (MQ=255)
                         gaAACCTTCAATCCCTGGCGCTCTTCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCg                                                                                                                                                                                                                                                               >  2:35364/1‑289 (MQ=255)
                         gaAACCTTCAATCCCTGGCGCTCTTCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCAACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACATGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCg                                                                                                                                                                                                                                                               >  2:301141/1‑289 (MQ=255)
                          aaaCCTTCAATCCCTGGCGCTCTTCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTg                                                                                                                                                                                                                                                                  <  1:35364/285‑1 (MQ=255)
                                         ggCGCTCTTCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCaaa                                                                                                                                                                                                                                               >  1:467487/1‑289 (MQ=255)
                                              tctTCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCaa                                                                                                                                                                                                                                          <  2:346726/289‑1 (MQ=255)
                                               ctTCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCaa                                                                                                                                                                                                                                          >  2:522063/1‑288 (MQ=255)
                                                 tCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGa                                                                                                                                                                                                                                       <  1:70677/289‑1 (MQ=255)
                                                          gCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCa                                                                                                                                                                                                                                                 <  1:54112/270‑1 (MQ=255)
                                                          gCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCa                                                                                                                                                                                                                                                 >  2:54112/1‑270 (MQ=255)
                                                            aaTCAAATTACTTGTTCCTGTCAGAGTCTGCAGGATCCCCTTGTCCCTCCACCCTGAGCCACTCCTCATTCTCGCGACAGACAGACTTCCCTGTTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGAGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGTCCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTTCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGt                                                                                                                                                                                                                            <  1:301815/289‑1 (MQ=255)
                                                                      cTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATa                                                                                                                                                                                                                                <  2:600882/275‑1 (MQ=255)
                                                                      cTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATa                                                                                                                                                                                                                                >  1:600882/1‑275 (MQ=255)
                                                                          cTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAAc                                                                                                                                                                                                              >  2:354485/1‑289 (MQ=255)
                                                                            tCTATCAGACTCTGATGAGTCCCCGTGGCCAGCCACGCGTAGCCACGCACCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATGATAAAAGTACAGACCTGTAACGGCGTAATGACTCTTTGGTTCTAACGGTTCTTCTTCCAGACTGATTGCCGTACCGTTTTTATCACACTCAACGACACCAAAGCGTTCTGGTCCAATAACGt                                                                                                                                                                                                            >  1:734408/1‑289 (MQ=255)
                                                                                 cAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAg                                                                                                                                                                                                       <  2:467487/289‑1 (MQ=255)
                                                                                     ctctGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCaa                                                                                                                                                                                                   >  1:241856/1‑289 (MQ=255)
                                                                                      tctGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCaaa                                                                                                                                                                                                  <  2:636283/289‑1 (MQ=255)
                                                                                              gTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGtt                                                                                                                                                                                           <  1:522063/288‑1 (MQ=255)
                                                                                               tCCCCGTGTCCAGCCCCGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTa                                                                                                                                                                                                                <  1:682787/266‑1 (MQ=255)
                                                                                               tCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCa                                                                                                                                                                                                                                                 <  1:320446/233‑1 (MQ=255)
                                                                                               tCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCa                                                                                                                                                                                                                                                 >  2:320446/1‑233 (MQ=255)
                                                                                               tCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTa                                                                                                                                                                                                                >  2:682787/1‑266 (MQ=255)
                                                                                                     tgtCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGGTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATa                                                                                                                                                                                                                                <  2:85236/244‑1 (MQ=255)
                                                                                                        ccAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCActttc                                                                                                                                                                                <  2:241856/289‑1 (MQ=255)
                                                                                                                              catcatCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCAtt                                                                                                                                                          >  2:344136/1‑289 (MQ=255)
                                                                                                                                                 cGTCCCTGCTCAAGATAAATACGTTTAACATCTGTACTTGCTACCTCACCACGTGCATACGTCTTCAAGTTTTTCGCCATCTGAACCCCGTCGTTATCATAAAAGTACAGACCTGTAACGGCTTAATTACTCTTTGCTTCTAACGGTTTTTCTCCCAGACTGATTGCCGTCCCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGTATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGa                                                                                                                                        <  1:301141/288‑1 (MQ=255)
                                                                                                                                                 cGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGt                                                                                                                                          <  2:900537/286‑1 (MQ=255)
                                                                                                                                                 cGTCCCTGATAAAGATAAATACGGTTAATATCTGTAATTGCTAACTCACCACGTTCAGACGGCTTCAAGTTTTTCTCCATCTGAACAACGTCGTTATCATAAAAGTACAGACCTGTAACGTCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGt                                                                                                                                          <  1:835108/286‑1 (MQ=255)
                                                                                                                                                                                 tGTAATTTATAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACAACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTCTTTCTTCCAGAATGATTGCCGTACCGGCTTTATAAAACTCAAAGACCCCATAGCGTGCCGGATTCTTACTGTGATAGGCAAATACCGTTGCACCACTTTCTTTGCTAACAGCGGCACCCTTTACCTCCGGCCGATCGTCACCTTAAAATATATCATCAcaacaacacaccgc                                                                                                        >  1:492621/1‑275 (MQ=255)
                                                                                                                                                                                   tAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGCCTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATATCGTTCTGGATCATTAACGTGATAGGTAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGACTCCATTAGCTTCGGCCGATCGATACCGTAAAAGAGTTTACCCCCAAGAACCCAAGaaca                                                                                                     >  1:398491/1‑289 (MQ=255)
                                                                                                                                                                                   tAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTAGCACCACTTTCTTTGTTAACTGCGTCCTCCATTAGCTTCGGCAGATTGTGACCGTAAAAGAAATTATCCACAAGAAcacacccaaa                                                                                                     >  1:50019/1‑280 (MQ=255)
                                                                                                                                                                                      tttCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCCACGACACCATAGCGTTCTg                                                                                                                                                                                                                        <  2:674050/171‑1 (MQ=255)
                                                                                                                                                                                         cTAACTCACCACTTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTGTTTATCAAACTCAACGACACCATAGCGTTCTGGGTCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAatcatc                                                                                               <  1:272025/289‑1 (MQ=255)
                                                                                                                                                                                                accacGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACCCCATAGCTTTCTGGAGCATTAAc                                                                                                                                                                                                              >  1:674050/1‑171 (MQ=255)
                                                                                                                                                                                                accacGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATa                                                                                                                                                                                                                                <  2:728906/153‑1 (MQ=255)
                                                                                                                                                                                                accacGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATa                                                                                                                                                                                                                                >  1:728906/1‑153 (MQ=255)
                                                                                                                                                                                                   acGTGCAGACGGCTTCAAGTTTTTCGCCATCTTAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCGGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCGTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATa                                                                                     <  2:529551/289‑1 (MQ=255)
                                                                                                                                                                                                     gTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCGTTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATaaa                                                                                   <  2:184317/289‑1 (MQ=255)
                                                                                                                                                                                                               cTTCAAGTTTTTCGCCATCTGAACCACGTCTGTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTTATAGGCAAATACCGTTGCACCACTTTCTGTGTTaa                                                                                                                                                                        <  2:246936/194‑1 (MQ=255)
                                                                                                                                                                                                                ttCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACTGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTAGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCga                                                                        <  2:50019/289‑1 (MQ=255)
                                                                                                                                                                                                                ttCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCGAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCtt                                                                              <  1:202926/283‑1 (MQ=255)
                                                                                                                                                                                                                ttCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCtt                                                                              >  2:202926/1‑283 (MQ=255)
                                                                                                                                                                                                                         tacGCCATATGAACCACGTCGTTATCATAAACGTACAGACCTGTAACGGCGTAATCACTCTTTGGTTCTAACGTTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCTATCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTACCAGCGGCCTc                                                                                                                                                              >  1:246936/3‑194 (MQ=255)
                                                                                                                                                                                                                                         cgtcgtTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCCAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATAATCACCACCAATAAACCATTCACCGATGATAAATGCCTGCGAGAGGCCac                                                >  1:19170/1‑287 (MQ=255)
                                                                                                                                                                                                                                                    tAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTCCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTGGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATAAATGCCTGCGCGAGGCCATCTGGGCTAGGtt                                    >  1:549047/1‑289 (MQ=255)
                                                                                                                                                                                                                                                           aCAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATAAATGCCTGCGCGAGGCCATCTGGGCTAGATTGCACt                               <  2:19170/287‑1 (MQ=255)
                                                                                                                                                                                                                                                               aCCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATAAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGt                           >  2:22689/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                  tGTAACGGCGTAATTACTCTTTGTTTCTAACGGTTTCTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACAGCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGAACGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACGCTTCACCGATGATAAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGa                      <  2:492621/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                       cGGCGTAATTACTCTTTGGTCCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTGGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATAAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGAtt                 <  2:549047/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    ctTTGGTCCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATAAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAgg             <  1:18865/280‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    ctTTGGTCCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATAAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAgg             >  2:18865/1‑280 (MQ=255)
                                                                                                                                                                                                                                                                                       tGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATAAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCt  >  2:281840/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                           
ACGAAATGCAATCTCTTCAGGACAGGAAACCTTCAATCCCTGGCGCTCTTCAATTGTCGCAATAAAATTACTTGCTTCTATCAGACTCTGATGAGTCCCCGTGTCCAGCCACGCGTAGCCACGCCCCATCATCGCGACAGACAGACGTCCCTGCTCAAGATAAATACGGTTAATATCTGTAATTTCTAACTCACCACGTGCAGACGGCTTCAAGTTTTTCGCCATCTGAACCACGTCGTTATCATAAAAGTACAGACCTGTAACGGCGTAATTACTCTTTGGTTCTAACGGTTTTTCTTCCAGACTGATTGCCGTACCGTTTTTATCAAACTCAACGACACCATAGCGTTCTGGATCATTAACGTGATAGGCAAATACCGTTGCACCACTTTCTTTGTTAACAGCGGCCTCCATTAGCTTCGGCAGATCGTGACCGTAAAAGATATTATCACCAAGAACCAAAGCACAATCATCACCACCAATAAACTCTTCACCGATGATAAATGCCTGCGCGAGGCCATCTGGGCTAGGTTGCACTTTGTACTGAAGATTCAGGCCCCACTGGCT  >  NC_000913/2110240‑2110806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: