Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,578,562 A→G 5.5% F259F (TTT→TTC)  mcrB ← 5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,578,5620AG 5.5% 251.6 / 3.4 73F259F (TTT→TTC) mcrB5‑methylcytosine‑specific restriction enzyme McrBC, subunit McrB
Reads supporting (aligned to +/- strand):  ref base A (32/37);  new base G (2/2);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

ATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATT  >  NC_000913/4578281‑4578842
                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                        
aTGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATg                                                                                                                                                                                                                                                                                    >  2:783518/1‑288 (MQ=255)
 tGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGcc                                                                                                                                                                                                                                                                                  <  2:181330/289‑1 (MQ=255)
                     aGGGCATAGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGa                                                                                                                                                                                                                                                              <  2:69080/289‑1 (MQ=255)
                        gCATAGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACg                                                                                                                                                                                                                                                            <  1:466784/288‑1 (MQ=255)
                                         cAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGccc                                                                                                                                                                                                                                          <  1:783518/289‑1 (MQ=255)
                                                  aTCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTTGGACGATAGCCCTGTATaaaa                                                                                                                                                                                                                                 >  1:447944/1‑289 (MQ=255)
                                                          tATTCCTTAAACCGAGTGTTTAAACATTCTCCGCGACATGGAATCGTTCTTCATCGTTTTCCGAGTAGGTTAGGGCAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATCGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCAt                                                                                                                                                                                                                          <  1:176067/288‑1 (MQ=255)
                                                              cATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGTGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGAACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCta                                                                                                                                                                                                                     <  1:703251/289‑1 (MQ=255)
                                                               aTTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCtat                                                                                                                                                                                                                    >  1:614123/1‑289 (MQ=255)
                                                               aTTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCtat                                                                                                                                                                                                                    <  2:140641/289‑1 (MQ=255)
                                                                 tAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATaa                                                                                                                                                                                                                  >  1:783301/1‑289 (MQ=255)
                                                                   aaCCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGa                                                                                                                                                                                                                <  1:805927/289‑1 (MQ=255)
                                                                        atgatATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAGAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTAATAGCTATAAGATTGAt                                                                                                                                                                                                           >  2:707939/1‑289 (MQ=255)
                                                                        atgatATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGcc                                                                                                                                                                                                                                           <  2:18448/257‑1 (MQ=255)
                                                                        atgatATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGcc                                                                                                                                                                                                                                           >  1:18448/1‑257 (MQ=255)
                                                                         tgatATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGa                                                                                                                                                                                                                                                <  2:825836/251‑1 (MQ=255)
                                                                         tgatATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGa                                                                                                                                                                                                                                                >  1:825836/1‑251 (MQ=255)
                                                                            tataAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGaa                                                                                                                                                                                                       <  2:650596/289‑1 (MQ=255)
                                                                            tataAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGaa                                                                                                                                                                                                       <  1:809323/289‑1 (MQ=255)
                                                                                     tctcCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGCAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTAAAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAAATGTAAAATAGGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGAAAGCCCTTTATACAATCCTCATAGCTATAAGAGTGATGGAACTAAAACaa                                                                                                                                                                                              >  2:841799/1‑288 (MQ=255)
                                                                                                      cGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATaaa                                                                                                                                                                                                                                  <  2:797810/236‑1 (MQ=255)
                                                                                                      cGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATaaa                                                                                                                                                                                                                                  >  1:797810/1‑236 (MQ=255)
                                                                                                         tcttcATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCt                                                                                                                                                                          <  2:826371/289‑1 (MQ=255)
                                                                                                             cATCGATTTCGGAGTAGGTTAGGGGCACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGACTTCATCTATAATATAAATATACTTTTTCTCTGCCTGCGCTTTAGCTTGCTGAAAAAAATGGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCACTTCGGACGATAGCCCTGTATAAAATCCTCTTAGCTCTTAGATTGAGGGACCTGAACCATATGTCCGCGTTGCGGAGCCtttc                                                                                                                                                                       >  1:125350/1‑287 (MQ=255)
                                                                                                                    ttCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTc                                                                                                                                                               >  2:474100/1‑289 (MQ=255)
                                                                                                                                        cAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCg                                                                                                                                           >  2:378382/1‑289 (MQ=255)
                                                                                                                                        cAGACCAGTTTTCACCTAGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAACATTGTAAAATATGCAGTCTTTACGTCGGAAGCAGACGCCATTCGGACGATAGCCCTGTCTAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGAAGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCTAGCCGGCg                                                                                                                                           >  1:416897/1‑289 (MQ=255)
                                                                                                                                                                    ttCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCg                                                                                                                                                                                >  1:585354/1‑224 (MQ=255)
                                                                                                                                                                    ttCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCg                                                                                                                                                                                <  2:585354/224‑1 (MQ=255)
                                                                                                                                                                          tAACATCATCACTTCGCCAAATACTTTACTGAGTTTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGAAGAAATTCGGACGATAGCCCGGTATAAAATCCTCATAGCTATAAGa                                                                                                                                                                                                                >  2:857878/1‑186 (MQ=255)
                                                                                                                                                                             catcatCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTgg                                                                                                      >  1:582739/1‑289 (MQ=255)
                                                                                                                                                                                           cAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATAttttt                                                                                        >  1:77168/1‑289 (MQ=255)
                                                                                                                                                                                              aTACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATAtttttttt                                                                                     >  2:664812/1‑289 (MQ=255)
                                                                                                                                                                                               tACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTg                                                                                    <  2:77168/289‑1 (MQ=255)
                                                                                                                                                                                                    tACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGTACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATTTTTTTTTTGATGGt                                                                               >  2:863807/1‑289 (MQ=255)
                                                                                                                                                                                                    tACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGt                                                                               >  2:497407/1‑289 (MQ=255)
                                                                                                                                                                                                    tACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGt                                                                               >  2:669036/1‑289 (MQ=255)
                                                                                                                                                                                                      cTGAGATTGGAACGATGGTGTTCATCTATAATAAAAATATACTTTTTCCCTGGATGCTCTTTAGCTTGCTGCCAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATGCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATTGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTa                                                                             <  1:497437/289‑1 (MQ=255)
                                                                                                                                                                                                        gagaTTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAGAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAAt                                                                           <  1:707939/289‑1 (MQ=255)
                                                                                                                                                                                                         agaTTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCg                                                                                                                                           >  1:765824/1‑224 (MQ=255)
                                                                                                                                                                                                         agaTTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCg                                                                                                                                           <  2:765824/224‑1 (MQ=255)
                                                                                                                                                                                                         agaTTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATc                                                                          >  1:683582/1‑289 (MQ=255)
                                                                                                                                                                                                            ttGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGtt                                                                       <  2:614123/289‑1 (MQ=255)
                                                                                                                                                                                                              ggCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGtt                                                                       <  2:116578/287‑1 (MQ=255)
                                                                                                                                                                                                              ggCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGtt                                                                       >  1:116578/1‑287 (MQ=255)
                                                                                                                                                                                                              ggCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTg                                                                     >  2:853176/1‑289 (MQ=255)
                                                                                                                                                                                                                cACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGaa                                                                                                                                                                                                  <  2:372869/162‑1 (MQ=255)
                                                                                                                                                                                                                cACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGaa                                                                                                                                                                                                  >  1:372869/1‑162 (MQ=255)
                                                                                                                                                                                                                   gattgattTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTAt                                                                <  2:582739/289‑1 (MQ=255)
                                                                                                                                                                                                                     ttgattTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTg                                                                                                       <  2:238397/248‑1 (MQ=255)
                                                                                                                                                                                                                     ttgattTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTg                                                                                                       >  1:238397/1‑248 (MQ=255)
                                                                                                                                                                                                                      tgattTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAATATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGt                                                             <  2:783301/289‑1 (MQ=255)
                                                                                                                                                                                                                                  taataaAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGaa                                                                                                                                                                                                       <  1:811565/139‑1 (MQ=255)
                                                                                                                                                                                                                                  taataaAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGaa                                                                                                                                                                                                       >  2:811565/1‑139 (MQ=255)
                                                                                                                                                                                                                                  taataaAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGt                                                 >  1:102119/1‑289 (MQ=255)
                                                                                                                                                                                                                                                   ttCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAGAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAg                                                                                                                                                                                                                 >  1:723431/1‑112 (MQ=255)
                                                                                                                                                                                                                                                   ttCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAGAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAg                                                                                                                                                                                                                 <  2:723431/112‑1 (MQ=255)
                                                                                                                                                                                                                                                            tgctCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAAc                        <  1:497407/288‑1 (MQ=255)
                                                                                                                                                                                                                                                             gctCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGc                      <  2:125350/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             gctCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGc                      <  1:474100/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             gctCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCAGTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGc                      <  2:856617/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             gctCTGTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGc                      <  2:416897/289‑1 (MQ=255)
                                                                                                                                                                                                                                                              ctctTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCa                     >  2:413943/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                      cTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGcaaca                                                                                                                                    >  1:572278/1‑170 (MQ=255)
                                                                                                                                                                                                                                                                      cTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGcaaca                                                                                                                                    <  2:572278/170‑1 (MQ=255)
                                                                                                                                                                                                                                                                       ttGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCa              <  1:853176/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                       ttGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATAGTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCa              <  1:664812/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGtct                                                           >  1:707180/1‑241 (MQ=255)
                                                                                                                                                                                                                                                                        tGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGtct                                                           <  2:707180/241‑1 (MQ=255)
                                                                                                                                                                                                                                                                         gCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGa                                                                                                                                                                                                                                                              <  1:370148/45‑1 (MQ=255)
                                                                                                                                                                                                                                                                         gCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGa                                                                                                                                                                                                                                                              >  2:370148/1‑45 (MQ=255)
                                                                                                                                                                                                                                                                                 aaaTTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGAtt  <  1:378382/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 aaaTTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGAtt  <  2:102119/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                        
ATGAAAGAAAATCGTCTGCGTAGGGCATAGTCAACAACGGCCAGAGAGCGATCGGCAGTATTCATTAAACCGATGATATAAACATTCTCCGGGACATAGAATCGTTCTTCATCGTTTTCGGAGTAGGTTAGGGGAACAGACCAGTTTTCACCTCGTTTATCATGTTCCATTAACATCATCACTTCGCCAAATACTTTACTGAGATTGGCACGATTGATTTCATCTATAATAAAAATATACTTTTTCTCTGGCTGCTCTTTAGCTTGCTGACAAAAATTGTAAAATATGCCGTCTTTACGTCGGAAGCCGACGCCATTCGGACGATAGCCCTGTATAAAATCCTCATAGCTATAAGATTGATGGAACTGAACCATATTGACGCGTTGCGGAGCCTTTTCTCCTGTCAGCAAGTAAGCCAGACGGCGTGCAACAAAGGTTTTTCCAACGCCGGGCGGCCCCTGGAGGATAATATTTTTTTTGATGGTTAATCGTTTGAGTATCGTCTCTATTGTGGTTTCAGGGATAAACAAATCATTTAACGCATCTTCCAGACAGTATGATT  >  NC_000913/4578281‑4578842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑