Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,527,947 C→G 3.9% T494R (ACA→AGA)  yrfF → putative RcsCDB‑response attenuator, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,527,9470CG 3.9% 487.1 / 7.6 145T494R (ACA→AGA) yrfFputative RcsCDB‑response attenuator, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (74/64);  new base G (4/2);  total (78/67)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 148 total aligned reads displayed.

TTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTG  >  NC_000913/3527659‑3528231
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                            
ttAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGAc                                                                                                                                                                                                                                                                                              >  1:846439/1‑289 (MQ=255)
  aGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACTACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAagagag                                                                                                                                                                                                                                                                                            <  1:319521/289‑1 (MQ=255)
  aGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAagagag                                                                                                                                                                                                                                                                                            <  1:869827/289‑1 (MQ=255)
  aGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAg                                                                                                                                                                                                                                                                                            <  2:871588/289‑1 (MQ=255)
      gTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGa                                                                                                                                                                                                                                                                                        >  2:251520/1‑289 (MQ=255)
               tgtgTAATATTCGAACTTCCGGAACCTGGATCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTc                                                                                                                                                                                                                                                                               <  1:195754/289‑1 (MQ=255)
                 tgtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCGCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCt                                                                                                                                                                                                                                                                              >  2:725914/1‑288 (MQ=255)
                 tgtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATTATTTTGGCGACATTGTACTGAAGACAg                                                                                                                                                                                                                                                                                            >  2:920814/1‑274 (MQ=255)
                 tgtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAg                                                                                                                                                                                                                                                                                            <  1:920814/274‑1 (MQ=255)
                 tgtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCt                                                                                                                                                                                                                                                                              >  2:231426/1‑288 (MQ=255)
                 tgtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCt                                                                                                                                                                                                                                                                              >  1:1358126/1‑288 (MQ=255)
                 tgtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTAAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGAGACATTGTACTGAAGACAGCGGATTTATGTTCt                                                                                                                                                                                                                                                                              >  1:1250429/1‑288 (MQ=255)
                  gtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTg                                                                                                                                                                                                                                                                             <  1:1314587/288‑1 (MQ=255)
                    aaTATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCGCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGcc                                                                                                                                                                                                                                                                           <  1:725914/288‑1 (MQ=255)
                    aaTATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGcc                                                                                                                                                                                                                                                                           <  1:699148/288‑1 (MQ=255)
                    aaTATTCAAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCGTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTCGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGcc                                                                                                                                                                                                                                                                           <  1:664089/288‑1 (MQ=255)
                       atTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAg                                                                                                                                                                                                                                                                       <  1:171507/289‑1 (MQ=255)
                           gAACTTCCGGAACCTGGAGTGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCCCATTGTCATTACCAGAATCTGAACTGGTCAACAAAGCGCCGGCATTGACTGAAGCAGTTAATCGCCAGCGTCACCCTAAACCGGAAGATGAATCTAGCGTCAGTGCCTCATTACGTTCAGCAATTTAACAATCCGGCATGGTCTTTCTTGATGATTCTGGCGACCTTGTACTGATGACAGCCGCTTTATGTTCTGCCAAAGATGa                                                                                                                                                                                                                                                                   >  2:148284/1‑289 (MQ=255)
                            aaCTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATg                                                                                                                                                                                                                                                                    >  1:647177/1‑287 (MQ=255)
                             aCTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGAGAGCGGATTTATGTTCTGCCAAAGATg                                                                                                                                                                                                                                                                    >  1:490491/1‑286 (MQ=255)
                             aCTTCCGGAACCTGGAGCGCGAAAACCAATGCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGAGGAATCTCGCGTCAGTGCCCCATTACGTTCAGCAATTCCAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACTTTTTACTCAAGACAACGGATTTATGTTCTGCCAAAGATg                                                                                                                                                                                                                                                                    >  2:575548/1‑286 (MQ=255)
                                tCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGCTGACtgtg                                                                                                                                                                                                                                                              <  1:284870/289‑1 (MQ=255)
                                         tGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCCTTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGCTGACTGTGTGCGACTGa                                                                                                                                                                                                                                                     >  1:1369627/1‑289 (MQ=255)
                                          gtggctcgAGAACCCATTCACCTGTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGaa                                                                                                                                                                                                                                                    <  2:478125/283‑1 (MQ=255)
                                             gcgcGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACTCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGaaaa                                                                                                                                                                                                                                                  <  1:1448471/288‑1 (MQ=255)
                                                 gAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAATCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATgcg                                                                                                                                                                                                                                             <  1:118840/289‑1 (MQ=255)
                                                                 tttttACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTAGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCa                                                                                                                                                                                                                             <  2:762281/289‑1 (MQ=255)
                                                                  ttttACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATg                                                                                                                                                                                                                                                                                  >  1:1157599/1‑235 (MQ=255)
                                                                         gTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTc                                                                                                                                                                                                                                                                               >  1:1203369/1‑231 (MQ=255)
                                                                         gTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTc                                                                                                                                                                                                                                                                               <  2:1203369/231‑1 (MQ=255)
                                                                         gTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTaaa                                                                                                                                                                                                                     >  1:85854/1‑289 (MQ=255)
                                                                         gTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTaaa                                                                                                                                                                                                                     >  2:348975/1‑289 (MQ=255)
                                                                                 gccaccAGAGCATCTGGAACGCCGCCCGCTCATTGCCATTACCAGATTCTGAACTGGTCAACAAAGCGACGGCCTTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCCGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATTACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGa                                                                                                                                                                                                             <  2:112078/284‑1 (MQ=255)
                                                                                  cTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGAc                                                                                                                                                                                                            >  1:305877/1‑289 (MQ=255)
                                                                                     gCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGAcgcg                                                                                                                                                                                                         <  1:131726/289‑1 (MQ=255)
                                                                                      cAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGAcgc                                                                                                                                                                                                          >  1:53340/1‑286 (MQ=255)
                                                                                         atcatcTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGCACTGGTCAACAAAGCGACGGCATTGAGTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTgg                                                                                                                                                                                                     >  1:114669/1‑289 (MQ=255)
                                                                                            atcTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTAGGCAACAGTAAAGACTGGGACGCGCTGGTaa                                                                                                                                                                                                  >  1:762919/1‑289 (MQ=255)
                                                                                                   acgacgCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACgcgc                                                                                                                                                                                           <  1:348975/289‑1 (MQ=255)
                                                                                                    cgacgCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGcc                                                                                                                                                                                          <  2:647177/289‑1 (MQ=255)
                                                                                                       cgCCGGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGGATTGCTTGATGATTTTGGCGCCATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAAc                                                                                                                                                                                       >  2:341695/1‑289 (MQ=255)
                                                                                                       cgCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAAc                                                                                                                                                                                       >  1:921250/1‑289 (MQ=255)
                                                                                                       cgCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACACTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAAc                                                                                                                                                                                       >  1:232396/1‑289 (MQ=255)
                                                                                                           cGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGAGAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCg                                                                                                                                                                                   <  2:490491/289‑1 (MQ=255)
                                                                                                           cGCTCATTGCACTTACCAGAATCTGGACTGTTCAACGAAGCGACGGCCTTGACTGAAGCAATTAATCGCCCGCTGCACCCTAAACCGGAAGTTGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGGTTTTGGCGACATTGTTCTGAAGACAGCGGGTTTATGTTCTACCAAAGATGGCTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTGAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCg                                                                                                                                                                                   <  2:356820/289‑1 (MQ=255)
                                                                                                                              aaTCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCCCGATGGCggtaa                                                                                                                                                                >  2:357595/1‑285 (MQ=255)
                                                                                                                               aTCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGa                                                                                                                                                                 <  2:616900/287‑1 (MQ=255)
                                                                                                                               aTCTGAACGGGTCAACAAAGCCACGGCATTGACTGAAGCATTTAATCGCCAGCTGCACCCTGAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGa                                                                                                                                                                 <  2:1244802/287‑1 (MQ=255)
                                                                                                                                    aaCTGGTCAACAAAGCGACGGCATCGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGtt                                                                                                                                                          >  2:121719/1‑289 (MQ=255)
                                                                                                                                      cTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAAAGTGttat                                                                                                                                                        <  1:7706/289‑1 (MQ=255)
                                                                                                                                         gTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTAc                                                                                                                                                     >  2:1264702/1‑289 (MQ=255)
                                                                                                                                         gTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCCCGCTGGCGTGAATGTGTTATTAc                                                                                                                                                     >  2:1370241/1‑289 (MQ=255)
                                                                                                                                           caacaaAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGAGAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACg                                                                                                                                                    <  1:291117/288‑1 (MQ=255)
                                                                                                                                                           ttGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGAAATTGTACTGAAGACAGCGGATTTATGTTCTGCACAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAAAAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAAAGCCGGGAAGCTCGCTGGCGTGAATGTGTTCTTCCGCACGGTGAGTGAGGAAt                                                                                                                                   >  2:1314960/1‑289 (MQ=255)
                                                                                                                                                            tGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAGCGCCGGGAAGCGCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATc                                                                                                                                  <  2:1358126/289‑1 (MQ=255)
                                                                                                                                                            tGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATc                                                                                                                                  <  1:581499/289‑1 (MQ=255)
                                                                                                                                                                  aaGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGATCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGAGTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGAAACAGTAAAGACTGGGACGCGCTGGTAAAACGCCCCAACGCCAGGAAGCCGGATGCCGTGGATGGGTTATTACGACCGGGGAGTGCGGAAACGCTGGa                                                                                                                            >  2:1315565/1‑289 (MQ=255)
                                                                                                                                                                        ttAATCGCCAGCTGCACCCTAAACCGGAAGATGAACCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGCCATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACCGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCCGAATCGCTGGATAAccg                                                                                                                      >  2:1047674/1‑288 (MQ=255)
                                                                                                                                                                            tCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCtggtg                                                                                                                  <  2:947441/289‑1 (MQ=255)
                                                                                                                                                                                cAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCa                                                                                                               >  1:407817/1‑288 (MQ=255)
                                                                                                                                                                                  gCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGa                                                                                                                                                                                                                                                                   <  2:891751/138‑1 (MQ=255)
                                                                                                                                                                                  gCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGa                                                                                                                                                                                                                                                                   >  1:891751/1‑138 (MQ=255)
                                                                                                                                                                                   cTGCACCCCAAACCGGAAGATGAAGTTCGCGTCAGTGCCTCATTTCGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTTCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCt                                                                                                           <  2:772680/289‑1 (MQ=255)
                                                                                                                                                                                    gtcacaCTAACCCGGAAGATGAATCCAGGGTCAGGTCAGAATTACGTTGAGCAATTAAAAAATCCGGCATGTTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTTTGCCAAAGATGACTGTGTGCGACTTAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAAc                                                                                                             <  2:232396/284‑1 (MQ=255)
                                                                                                                                                                                             aaCCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTTGCAACCTCCACCgcgc                                                                                                  >  2:44148/1‑288 (MQ=255)
                                                                                                                                                                                                   aaGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTa                                                                                            >  1:112285/1‑288 (MQ=255)
                                                                                                                                                                                                   aaGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTa                                                                                            >  1:1286334/1‑288 (MQ=255)
                                                                                                                                                                                                        gAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGc                                                                                      <  1:1264702/289‑1 (MQ=255)
                                                                                                                                                                                                                cgTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACg                                                                              >  1:1277555/1‑289 (MQ=255)
                                                                                                                                                                                                                        ccTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGc                                                                      <  2:921250/289‑1 (MQ=255)
                                                                                                                                                                                                                         cTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGc                                                                      <  2:1286334/288‑1 (MQ=255)
                                                                                                                                                                                                                         cTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCg                                                                     >  1:138038/1‑289 (MQ=255)
                                                                                                                                                                                                                             ttactgtCAGAAATTCAACAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGcac                                                                 <  2:305877/283‑1 (MQ=255)
                                                                                                                                                                                                                             tgaCGTTGAGCTATTCAAAAATCCGGCATGGTATTGCTTGATGATTTGGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCAAAAGATGACTGTGTGCGACTGAAAAAGGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGcac                                                                 <  2:762919/287‑1 (MQ=255)
                                                                                                                                                                                                                                   tCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGcgcggtggaggttgcca                                                            <  1:243464/288‑14 (MQ=255)
                                                                                                                                                                                                                                   tCAGCAATTCAAAAATCCGGCATGGTATTGCTCGATGATTTTGGCGCCATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTTTGCGACTGAAAAATGCGCTGGTCAATCTCGGCCACAGTAAAGACCGTGACGACCTGCTAAAACGCGCCAACGCCGGGAAGATCGATGGCGTGAACGTGTTATTAAGCCAGGTGAGTCCGGAATCGCTGGTTAACCTGGCGGCAACACCCACCTCGCCGTTTATACCGCATAAACCGGCGCCAGCCGCACAATc                                                             >  2:934527/1‑287 (MQ=255)
                                                                                                                                                                                                                                    cAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGAGAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAACCGGCGCGAGCGGCCCAATAAcc                                                          >  2:1105436/1‑288 (MQ=255)
                                                                                                                                                                                                                                     aGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTa                                                         >  1:950321/1‑289 (MQ=255)
                                                                                                                                                                                                                                       cAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCa                                                                                                                                                                                                                             <  1:971828/123‑1 (MQ=255)
                                                                                                                                                                                                                                       cAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACATGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTaa                                                        >  1:329331/1‑288 (MQ=255)
                                                                                                                                                                                                                                                    cGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAAAACGATGGTCAATATCGGCAAAAGTAAAGACTGGGACGCGATGGTAAAACGCGGCAACACCGGGAAGCTCGATAGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCCCACTCCAGAAAAAGTCCAGcccc                                           >  2:809975/1‑288 (MQ=255)
                                                                                                                                                                                                                                                           gTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGAt                                   >  2:1355044/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                 cTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGccc                                            <  2:461783/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                 cTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAACCACTAACCAGTCCCGCCCCCGGCGGATTCCTGa                             >  2:854200/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                  ttGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGa                             <  1:341695/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                    gatgatTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCCCCGCTTATCACGCATGAAACGGCGCGAGCGGCCCACTCACTAAACAGTCCGGCCCCCGGCGGAATCCTgttcg                          >  1:515383/1‑285 (MQ=255)
                                                                                                                                                                                                                                                                     atgctTTTGGCGACATTGTACTGCAGAGAGCGGATTTATGTGCTGCCAAAGATGACTGTGTGCGACTGAACAATGCGCTGGTCAATCTTGGCAACACTCCAGACTGCGAGGCGCTGCTAACACGCGCCAACGCAGGGCAGCTCGACGGCGGGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGCTAACCTGGTGGCCACCACCCCCGCGCGGTTTACCACGCATGAAATGGGGCGAGCGGCACAATCCAGACACAGACCGCCCCCaggcgacatcctgctctt                         >  2:245904/1‑276 (MQ=255)
                                                                                                                                                                                                                                                                      tgatTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCCCAATCACTAAACAGTCCGGCCCCCGGCGGGCTCCTGATTTTc                        >  2:692214/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                      tgatTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGt                         >  2:1280249/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                      tgatTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCAAAATCACTAAACAGTCCGGCCGCCGGCGGATTCCTGATTGt                         >  1:614544/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                          tttGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGcg                    >  1:622350/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                           ttGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGcg                    >  2:1380018/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                   ctggTACTCAAGAAAGCTGAGTTATGTTATGCGAAAGATGACTGCGTGCGACTGAAAAAGGCGCTGGTCAAGCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTGGATGGCGTGAATGTGTTATTACGCACGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAg          <  2:85854/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        aCTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGAt      >  1:142316/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                            aaGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTttg  >  1:642373/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                            aaGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTttg  >  1:44871/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                              gACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGtttcat                                                                                          >  1:205831/1‑196 (MQ=255)
                                                                                                                                                                                                                                                                                              gACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGtttcat                                                                                          <  2:205831/199‑4 (MQ=255)
                                                                                                                                                                                                                                                                                              gACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGcac                                                                 >  1:71823/1‑224 (MQ=255)
                                                                                                                                                                                                                                                                                              gACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGcac                                                                 <  2:71823/224‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                            
TTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTG  >  NC_000913/3527659‑3528231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: