Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,223,733 Δ1 bp 4.0% coding (1103/3093 nt) ebgA → evolved beta‑D‑galactosidase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,223,7320A. 4.0% 548.4 / 11.4 151coding (1102/3093 nt)ebgAevolved beta‑D‑galactosidase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base A (71/72);  new base . (4/2);  total (76/75)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01

Only 100 of 155 total aligned reads displayed.

ACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGA  >  NC_000913/3223444‑3224020
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                 
        cAGCTTTGCTTTTACTGTCTAACGGCCGCAGCAATGGTCATCAGAATCCCCTTATCTTTACCGTCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAAc                                                                                                                                                                                                                                                                                           <  2:1038264/289‑1 (MQ=255)
        cAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTAGCTTTACCATCTGGTCATAACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTTCCACAACGCGTTGTCTTCCGTGATATCAAAGTGCGAGACGGTCTGTTCTGGATCAATACCCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAAc                                                                                                                                                                                                                                                                                           <  2:74106/289‑1 (MQ=255)
             ttgctttTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGt                                                                                                                                                                                                                                                                                      <  2:381281/289‑1 (MQ=255)
                 ttttACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCg                                                                                                                                                                                                                                                                                   <  2:821168/288‑1 (MQ=255)
                         tCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGACCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGTGAAAGCTATCCAGTTGATGAACCAGCACAATATCAAATCCGTGCGTACCGCTc                                                                                                                                                                                                                                                                           >  1:451222/1‑288 (MQ=255)
                          cGAGCAGCCGCAGCAATGGTCAGCTCAATCCCCTTATATGTACCATCTGGTCATGACGCTGAAAGAAGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGGGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCa                                                                                                                                                                                                                                                                          <  1:371953/288‑1 (MQ=255)
                                   gcagcaATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGaa                                                                                                                                                                                                                                                                 >  1:1545126/1‑288 (MQ=255)
                                                      tCCCCTTCTCTTTACCATCAGGTCATGACGCTGAACGACGCCACCGGTAACGTTCTGTATATGGTCCCACCACGCGTTGGCTTCCGGGAGAACACAGTGCGCGACGGTCTGTTCTGGAACAATAACCGTTATGTGATGCTGCTCGGCATCAACCTTCACGTGAACGATCATCGCAACGACCGCGCCGTTGGAATGGATCCCGTCGAGAAAGATCTCCAGTTGATGAAGCAGTACAATATCAACTCCGTTCGTCCCGCTCACTACCCGAACGATCCGCGTTTTTACGaca                                                                                                                                                                                                                                             >  1:699422/1‑287 (MQ=255)
                                                      gccccTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAATTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTACACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGaa                                                                                                                                                                                                                                                                 <  2:940635/268‑1 (MQ=255)
                                                           ttATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgtgt                                                                                                                                                                                                                                        >  1:1387444/1‑289 (MQ=255)
                                                             aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGa                                                                                                                                                                                                                                      <  2:1449753/289‑1 (MQ=255)
                                                                tttACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCGTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGAAGCACAATATCAACTCCGTTCGTACCGCTAACTACCCGAAAGATCCGCGttctggctcctctacaca                                                                                                                                                                                                                                      >  1:940635/1‑270 (MQ=255)
                                                                  tACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCTTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGAGTGATATCt                                                                                                                                                                                                                                 >  2:163262/1‑289 (MQ=255)
                                                                   aCCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgt                                                                                                                                                                                                                                          >  2:1470485/1‑279 (MQ=255)
                                                                   aCCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACtgt                                                                                                                                                                                                                                          <  1:1470485/279‑1 (MQ=255)
                                                                   aCCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCt                                                                                                                                                                                                                                 >  1:219817/1‑288 (MQ=255)
                                                                   aCCATCCTGTCAGGACGGTGAAAGACGCCAACGGCAACGTTATGGAAGGGGTGGCACAACGCGTTGGCTTTGGTGATATCCAAGTTCGCGACCGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTa                                                                                                                                                                                                                                <  1:36120/289‑1 (MQ=255)
                                                                     cATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACg                                                                                                                                                                                                                              <  2:643745/289‑1 (MQ=255)
                                                                       tCTGGTCATGACGCTGAAAGACGCCAACGGGAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTAAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGc                                                                                                                                                                                                                            <  1:1053814/289‑1 (MQ=255)
                                                                       tCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGaa                                                                                                                                                                                                                                              <  2:258239/271‑1 (MQ=255)
                                                                       tCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGaa                                                                                                                                                                                                                                              >  1:258239/1‑271 (MQ=255)
                                                                        cTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACgg                                                                                                                                                                                                                             >  2:714722/1‑287 (MQ=255)
                                                                        cTGGTAATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCt                                                                                                                                                                                                                                                                            <  1:1555380/240‑1 (MQ=255)
                                                                        atGGTCATGACGCTGAAAGACGCCAACGTCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCAGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAAACGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGACAGATCTACAGTTGATGAAGCAGCCAAATATCAACTCCGTGCATACCCACAACTCCCCGCACGATCCGCGTCTTTACGAACGGTGTGATATCTACgcg                                                                                                                                                                                                                            >  1:1398108/2‑286 (MQ=255)
                                                                                 aCGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGAt                                                                                                                                                                                                                 >  2:1428329/1‑289 (MQ=255)
                                                                                            cGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCAACGGCCTGTTTGTGACGGCGGaaccg                                                                                                                                                                                                       >  1:1419201/1‑286 (MQ=255)
                                                                                                          ttCGGGAGGTGTGGCCACAACGCGTTGGTTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTTGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCa                                                                                                                                                                                         <  1:494383/289‑1 (MQ=255)
                                                                                                           tCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCa                                                                                                                                                                                         <  2:787538/288‑1 (MQ=255)
                                                                                                             tGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACt                                                                                                                                                                                                                                            >  1:1246058/1‑235 (MQ=255)
                                                                                                             tGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACt                                                                                                                                                                                                                                            <  2:1246058/235‑1 (MQ=255)
                                                                                                               gAAGTGGTACCACAACGCGTTGGCTTCCGGGATATCAAGGTGCGCGACGGTCGGTTCTGGGTCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCCAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGc                                                                                                                                                                                     <  1:171408/288‑1 (MQ=255)
                                                                                                                    ggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGc                                                                                                                                                                                                                            >  1:716730/1‑244 (MQ=255)
                                                                                                                    ggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCa                                                                                                                                                                                         <  2:1373857/279‑1 (MQ=255)
                                                                                                                              cgcgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGACGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCCCCAGTTGATGAAGCAGCCCAATATCAACTCCGTGCGTACCGCTCACTACCCGACCGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCCCGGCTTTGCTAATGTCCGca                                                                                                                                                                     >  1:1501140/1‑288 (MQ=255)
                                                                                                                                cgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGatat                                                                                                                                                                                                                                   >  2:1142429/1‑225 (MQ=255)
                                                                                                                                cgTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGat                                                                                                                                                                   >  1:1006152/1‑289 (MQ=255)
                                                                                                                                 gTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGata                                                                                                                                                                  <  2:1014285/289‑1 (MQ=255)
                                                                                                                                   tGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATAtt                                                                                                                                                                <  2:1006152/289‑1 (MQ=255)
                                                                                                                                          cGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATc                                                                                                                                                                                                                                                                                              <  1:1385668/156‑1 (MQ=255)
                                                                                                                                          cGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATc                                                                                                                                                                                                                                                                                              >  2:1385668/1‑156 (MQ=255)
                                                                                                                                            ttatatCAAATTTACCGACGGCCGTTTCGTGATCAATACCCGTGAGGTGTGGCGGCCCGGCGGCAAACGTCCGTACAACGATCTGCGCAAAGGCCGCGCCGTTGGAATGGCTCCCGTCGAGAAAGATCTTCAGTTGATGAAGCAGCACAATATCAACTCCGTGCTGACCTCTCACTACCCGGACGATCCGCGGTTTGACGAACTGTGTGATCTCTACGGCCTGTTGTGGAAGGCTGAAACCGACGTCGAATCTCACGGCTTTGCTAATGTCGGCGAGATTAGCCGTAtt                                                                                                                                                       <  1:917351/287‑1 (MQ=255)
                                                                                                                                                         cgcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCCCCGCGTCAACCGTCACGACAACGATCATCGCAAACGCCGCGCCGTTGACATGGATCGCGTCGAGAAAGCTCTCCAGTTGATGAAGCAGCACAATCTCCACTCCGTTCGTACCGCCCACTCCCCGAACGATCCGCGGTTTTCCGGACTGCGTGATATCTATTGCCTGTTTGGGATGGCGGTAACCGACGTCGAAACGCACGGCTTTGCTACTGCCGGCTCAACTTGCCGTATTACACACCATCCg                                                                                                                                           >  1:456064/1‑288 (MQ=255)
                                                                                                                                                         cgcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCg                                                                                                                                           >  2:334731/1‑288 (MQ=255)
                                                                                                                                                         cgcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCg                                                                                                                                           <  2:1545126/288‑1 (MQ=255)
                                                                                                                                                         cgcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGc                                                                                                                                          >  2:1141620/1‑289 (MQ=255)
                                                                                                                                                                  cTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGaa                                                                                                                                  <  2:763023/288‑1 (MQ=255)
                                                                                                                                                                     tgcTGGATCAATAACCGTTAGGTGGTGGTGGACGGCGTGCACCGTCACGACAACGATCATCGCAAGGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTAATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGaa                                                                                                                                  <  1:1182588/283‑1 (MQ=255)
                                                                                                                                                                          gATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCTCAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGGTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTCCCGACGAACCGCAGTGGGAAAAAGTCGAc                                                                                                                         >  1:1007796/1‑289 (MQ=255)
                                                                                                                                                                               aaaaCCGTTATGTGATGCTGCCCGGCGTCAACCGACACGACAACGATCAGCGCAAAGGGCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGa                                                                                                                    <  1:1140373/287‑1 (MQ=255)
                                                                                                                                                                               aTAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGa                                                                                                                    >  2:184740/1‑289 (MQ=255)
                                                                                                                                                                               aTAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGGATTACTGACGAGCCGCAGGGGGAAAAAATCTACGTCGa                                                                                                                    >  2:1226176/1‑289 (MQ=255)
                                                                                                                                                                                       tttgtgATGCGGCACGGCGTCAACCGTCACGACAACGAGCATCGCAAAGGCCGCGCCGTTGGAATGGATCGGGTCGAGAAAGATCTCCAGTGGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCCCACTACCCGAACGATCCGCGTTTTTACGAACTGTGGGATATCTCCGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTg                                                                                                            <  1:1254010/287‑1 (MQ=255)
                                                                                                                                                                                        aTGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACTGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGt                                                                                                           >  2:621604/1‑289 (MQ=255)
                                                                                                                                                                                             aTGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATAGCACGGCTTTGCTAATGTTGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGc                                                                                                       >  2:1253004/1‑288 (MQ=255)
                                                                                                                                                                                              tgctgcACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCgg                                                                                                                                                                       <  1:199322/223‑1 (MQ=255)
                                                                                                                                                                                              tgctgcACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCgg                                                                                                                                                                       >  1:1149341/1‑223 (MQ=255)
                                                                                                                                                                                              tgctgcACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCgg                                                                                                                                                                       >  2:199322/1‑223 (MQ=255)
                                                                                                                                                                                              tgctgcACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCa                                                                                                     >  2:165666/1‑289 (MQ=255)
                                                                                                                                                                                              tgctgcACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCa                                                                                                     >  2:588651/1‑289 (MQ=255)
                                                                                                                                                                                              tgctgcACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGAGCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCa                                                                                                     >  2:1054698/1‑289 (MQ=255)
                                                                                                                                                                                                   cACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATAtt                                                                                                                                                                <  1:132592/225‑1 (MQ=255)
                                                                                                                                                                                                   cACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTCCGTCGAGCGCATTTTTCGCCATAGcc                                                                                               >  2:410293/1‑289 (MQ=255)
                                                                                                                                                                                                   cACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGGTTTACCGACGATCCGACGCGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCatcac                                                                                                 >  1:817694/1‑285 (MQ=255)
                                                                                                                                                                                                        cGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGc                                                                                                                                                                                                                            <  2:242708/160‑1 (MQ=255)
                                                                                                                                                                                                        cGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGc                                                                                                                                                                                                                            >  1:242708/1‑160 (MQ=255)
                                                                                                                                                                                                         gTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCAcgcg                                                                                          <  2:1419201/288‑1 (MQ=255)
                                                                                                                                                                                                         gTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCAcgcg                                                                                          <  1:184740/288‑1 (MQ=255)
                                                                                                                                                                                                          tCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCGAATGTCGGCGATATTAGCCGAGTAACCGACGATCCGCGCAGGGAAAATGTCTACGTCTAGCGCATTGTTCGCCCTATGCAcgccc                                                                                         >  1:101961/1‑286 (MQ=255)
                                                                                                                                                                                                                  cACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAAc                                                                                 <  2:1277786/288‑1 (MQ=255)
                                                                                                                                                                                                                    cGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCAt                                                                              <  2:60186/289‑1 (MQ=255)
                                                                                                                                                                                                                       acaagttaatgccAAAGGCAGGGCCGTTGGAATGGATCGCGTCGAGAAAGTGCGCCAGTTGACGAAGCAGCACAATATCAAATCCGTGCGTACCGCTCACTACCCGAACGTTCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAATCCATCCg                                                                           <  1:788701/277‑1 (MQ=255)
                                                                                                                                                                                                                        aaCGATCATCGCAAAGGCCGGGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTAGATGAAGCAGCACAATATCAACTCCGTGCGTACCTCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGt                                                                          <  2:1166562/289‑1 (MQ=255)
                                                                                                                                                                                                                        aaCGATCATCGAAGAGGCCGCGCCGTTGGAATGGATCACGTCGGGAAAGATCTCCAGTTGAGGAAGCAGCACAATATCAACGCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGt                                                                          <  1:749233/289‑1 (MQ=255)
                                                                                                                                                                                                                                  gCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGc                                                                                                                                                                                     <  1:528951/173‑1 (MQ=255)
                                                                                                                                                                                                                                  gCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGc                                                                                                                                                                                     >  2:528951/1‑173 (MQ=255)
                                                                                                                                                                                                                                      aGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTTATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGACAAAGTCTACGTCGAGCGCATTGTTCGCCATATCGCACTGCAGAACAACCAACCGtgcaatctcctctgg                                                            >  1:1443030/1‑275 (MQ=255)
                                                                                                                                                                                                                                                gTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCa                                                  <  2:1305619/289‑1 (MQ=255)
                                                                                                                                                                                                                                                  tGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAAt                                                >  2:841132/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      tagtaTCGCGGCAAGAAAGGTCTCCAATTGATGAAGCAGCACAATGTCAGCTCCGTGCGTACCGCTCACTACCCTAACGATCGGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCTCACGGCTTTGCTAATGTCGGCTATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAAt                                            <  1:621604/285‑1 (MQ=255)
                                                                                                                                                                                                                                                      aTGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGaa                                             >  2:436929/1‑288 (MQ=255)
                                                                                                                                                                                                                                                         gATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGTAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTTGAGCGCATAGTTCGCCATATCCACGCGCAGAAAAACCATCCGTGGATCCTCTTCTGGTCGCACGGCACAGAATCCg                                         >  1:980071/1‑289 (MQ=255)
                                                                                                                                                                                                                                                         gATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGtt                                                                                                          >  1:604086/1‑225 (MQ=255)
                                                                                                                                                                                                                                                         gATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGtt                                                                                                          <  2:604086/225‑1 (MQ=255)
                                                                                                                                                                                                                                                         gATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCg                                         >  2:1463682/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            cgcgTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCACCTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCt                                      <  2:1152546/289‑1 (MQ=255)
                                                                                                                                                                                                                                                            cgcgTCGAGAAAGATCGCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAAGGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCGAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCt                                      <  2:699422/289‑1 (MQ=255)
                                                                                                                                                                                                                                                              cgTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCt                                      >  1:708152/1‑287 (MQ=255)
                                                                                                                                                                                                                                                              cgTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCCACGATCCTCAGGGGGAAAAAGTCCACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGCACATACTCTGGCCGCTGGGCAATGAACCCGGCTAt                                    >  1:1152546/1‑289 (MQ=255)
                                                                                                                                                                                                                                                              cgTCGAGAAAGATCTCCAGTTGATGAAGCAGCACATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTATGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGCAAATGAATCCGGATATg                                   >  1:1358456/1‑289 (MQ=255)
                                                                                                                                                                                                                                                              cgTCGAGAAAGATCTCCAGTTGATGAAGCAGCACATATCAACTCCGTACGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGCAACCGACGTAGAATCGCACGGCTTTGCTAATGTCTGCGATATTAGCCGTATTACCAACGAACCGCAGTGGGACAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATg                                   >  1:1520435/1‑289 (MQ=255)
                                                                                                                                                                                                                                                              cgTCGAGAAAGATCTCCAGTGGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCt                                      <  2:708152/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                           ctcCAGTTGATGAAGCAGCAGAAGATCAACTCCGAGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATcc                       <  2:456064/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                           ctcCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATcc                       <  2:786918/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                            tcCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCcg                      <  2:1443030/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   gatgaAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCAGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCGGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACAATCGCCCGATg               >  1:652489/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                     tgaAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTa                                     <  2:328256/265‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     tgaAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTa                                     >  1:328256/1‑265 (MQ=255)
                                                                                                                                                                                                                                                                                        agaagcaCAATTTGAACTCCGTGCGTACCGCTCACTACCCGATCGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCa          <  1:1141620/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                                             cacaATATCAACTACGTGCGTACCGCTCACTACCCGCACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACCATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCCCGAGCAGAAAAACAATCCGTCTATCACAATATGGt                                                           >  1:1455586/1‑235 (MQ=255)
                                                                                                                                                                                                                                                                                                aaTATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGa  <  2:473910/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                 
ACCAGCGCCAGCTTTGCTTTTACTGTCGAACAGCCGCAGCAATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGCAGCACAATATCAACTCCGTGCGTACCGCTCACTACCCGAACGATCCGCGTTTTTACGAACTGTGTGATATCTACGGCCTGTTTGTGATGGCGGAAACCGACGTCGAATCGCACGGCTTTGCTAATGTCGGCGATATTAGCCGTATTACCGACGATCCGCAGTGGGAAAAAGTCTACGTCGAGCGCATTGTTCGCCATATCCACGCGCAGAAAAACCATCCGTCGATCATCATCTGGTCGCTGGGCAATGAATCCGGCTATGGCTGTAACATCCGCGCGATGTACCATGCGGCGA  >  NC_000913/3223444‑3224020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: