Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,296,154	T→C	15.4%	intergenic (+361/+283)	gltP → / ← yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,296,154	0	T	C	15.4%	59.0 / 6.7	39	intergenic (+361/+283)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (16/16);  new base C (3/3);  total (20/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

CGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTA  >  NC_000913/4295870‑4296434                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                         cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCACTAGCCTGATTTCGCGTGCTTTTGTAGGTCGGACAAGGCGTTTACGCCCCATCCGACATCAACCCCTGGTGCGACGCTTGCCGCCTCTTATCAAGACTACGCCAGACCTGCCACAATCTTGCTTAACCGTGATTTTGTTGGCCCGATTCGGCGTTTATGcc                                                                                                                                                                                                                                                                                       >  1:1317085/1‑288 (MQ=11) cGCCAGACAGAGCAATGGCCTGATTTAGCGTGATTTGGTAGAGCGGTTAATGCTTTTATGCCGCATCGTACATCAACGCCTGATGCGACGCGTAACGGGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAGGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg                                                                                                                                                                                                                                                                                      <  1:683887/289‑1 (MQ=11)      gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGCTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCTACGCTTGCCGCCTCTTATCAGGCCTACTCCCCACTGCGCAATATCCTGTATTCGCATTATTTTGTAGGTCGGATCAGGCGTTTCTGccccgtc                                                                                                                                                                                                                                                                                  >  1:1202673/1‑283 (MQ=11)                     tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCt                                                                                                                                                                                                                                                                    >  1:301534/1‑287 (MQ=35)                           aGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATACGACATCAACTCCTGATGCGAc                                                                                                                                                                                                                                                            >  2:522037/1‑289 (MQ=25)                                 aTTTGGTAGGTCGGATGAGGGGTTTATGCCGCATCCGAAATCAACGCCTGATGGGAGGCTTAACGGGTCTTATCAGGCCTACGCCAGACGGCGCAATAGCCTTATTTGGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                                                                                                                      <  1:1280364/289‑1 (MQ=11)                                  ttttgttgtgtggagtaGGGGTTGATGGCGCATGCGACACACACGCCGGATGCGACGCTGAACGCGTCTTATAAGGGCTACGCCAGACAGCGCAATAGCCTCATTTAGCGTGATTTTGGAGGTCGGATAAGGCTTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                                                                                                                      <  1:1141241/272‑1 (MQ=11)                                  ttttGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                                                                                                                      <  2:1452829/288‑1 (MQ=37)                                                tAAGGGGTTTAGACCGAGTACGGCATAAATGCCGGATGAGACGCTTAACGCGTGTGATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                       <  1:742496/289‑1 (MQ=11)                                                tAAGGCGTTTATGCCGCGTCCGACATCAACGCCTGAGGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                       <  2:803136/289‑1 (MQ=37)                                                tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                       <  2:301534/289‑1 (MQ=37)                                                   ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCACGCCTACGTCAGACAGCGAAATAGCCTGATCTAGCTTGATTTTTTAGGTCGGATCAGGCCATTCCGCTGCATCCCACACCAACGCCTGATGCGACACATACCTCGTCTTACCTGGCCt                                                                                                                                                                                                                                     >  1:173405/1‑288 (MQ=11)                                                     cGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCTAGACAGTGCATTAGCCTGCTTTCGCGTGATTTTGTAGGTCGGATACGTCGTTTAAGCCTCATCCGACCTCCACGCCTGATGTGTCCCTTAACGCGTCCTATCAGGCCtcc                                                                                                                                                                                                                                   >  1:544924/1‑286 (MQ=11)                                                      gTTTATGCCGCAGCAGAGATCAACTCCTTAGGCGACGTTTGCCGCGTCTTATGAGTCATACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACg                                                                                                                                                                                                                                  <  1:1017172/288‑1 (MQ=11)                                                              cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAgc                                                                                                                                                                                                                         >  1:764687/1‑289 (MQ=25)                                                                  atcggaagcAACGCCTGGAGCGACGCTACGTGCGTCGTAGTAGGCATACGCCAGATGGGACAATGGCCTGATTGGGAGTGATTTTGTGAGACGTATAAGGCGTTTATGCCGCATCCGACATCATCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATGCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                                                                                                                     <  1:939544/281‑1 (MQ=11)                                                                                        aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt                                                                                                                                                                                               >  2:1105173/1‑289 (MQ=35)                                                                                        aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAtttt                                                                                                                                                                                               >  2:108445/1‑289 (MQ=35)                                                                                                        aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                                                                                                                     <  2:1473838/251‑1 (MQ=33)                                                                                                              gccaCGCCGGACAGGGGAATGGCGTAATGAAGCGTGATTTTGTAGGTCGGATAAGACGTTGAGTCCGACTACGAGATAAACGCGTTATTCGATGCTTGCCGCGTCTTATCAGGCTTACTAGAGAAAACGCAATAGCCTGATTGAGCGGGATTTAGTAGGTCTGATAAGGAGTGTATGCCGGATCTGAGAGTAACGCCTGATGCGACGCTTAACGCGGCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTa                                                                                                                                                                          <  1:737997/285‑1 (MQ=2)                                                                                                                  cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCTGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAtt                                                                                                                                                                                                           >  1:1473838/1‑251 (MQ=18)                                                                                                                            cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                                                                                                                     <  2:334061/231‑1 (MQ=33)                                                                                                                            cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                                                                                                                     >  1:334061/1‑231 (MQ=33)                                                                                                                                 tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGc                                                                                                                                                                                                                                                               >  1:1427941/1‑184 (MQ=35)                                                                                                                                 tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGc                                                                                                                                                                                                                                                               <  2:1427941/184‑1 (MQ=35)                                                                                                                                      tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACa                                                                                                                                                                                                                           >  2:4930/1‑215 (MQ=34)                                                                                                                                      tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACa                                                                                                                                                                                                                           <  1:4930/215‑1 (MQ=34)                                                                                                                                      tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGATTAACGCGTCTTATCAGGCCTACTCCAACCTGCGCAATAGCCTGCTTTAGCGTGATTCTGCATGTCGGATAAGGCGTTTCAGCCGCATCCGACATCAATGccggt                                                                                                                                                 >  1:757837/1‑286 (MQ=11)                                                                                                                                            aGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATg                                                                                                                                                                                                                                                                                         >  1:1023374/1‑147 (MQ=37)                                                                                                                                                             cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAAAGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGGTTTATCCTGATTTTGTAGGCCGGATCAGGCGATTCCGCCGCATCCGACCTCAATGGCTGATGGGCCGCGTCACGCGTCTTacc                                                                                                                          >  1:244487/1‑287 (MQ=11)                                                                                                                                                                      cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                       <  2:1194619/171‑1 (MQ=35)                                                                                                                                                                          taagccgcATCCGACATCAACGCCTGATGCGAAGCTTGCCGCGTCTTATCAGGCCTAAGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGACGTTTATGCATCATCCGACATCAACACCAGCTGCGACGCTTAACGCGTCTTATCAGGCCTAAGCCAGACAGCGCACTAGCCTGACTTAGCGTGATTTTGTAGCTCGGATAAGGCGTTTACGCCGCATCCGACAGCAATGCCTGATGAGACGCTTACCGCGCTTTCTCCGGCCttcctta                                                                                                              >  1:1483541/4‑282 (MQ=1)                                                                                                                                                                                gcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGa                                                                                                                                                                                                                             >  1:1194619/1‑171 (MQ=33)                                                                                                                                                                                                                                              caaaTTGCCTGATTTAGCTGGAGTTTGTAGGTCGGATCAGGCGTTTTTTCCGCATCAGGCATCAACGCCTGATGCGACGCTTAACGGGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                              <  1:1414273/282‑1 (MQ=11)                                                                                                                                                                                                                                                                        gTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgt               >  2:777164‑M2/1‑263 (MQ=255)                                                                                                                                                                                                                                                                              cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttatc         <  1:777164‑M2/289‑33 (MQ=255)                                                                                                                                                                                                                                                                              cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttat          >  2:1079188‑M2/1‑257 (MQ=255)                                                                                                                                                                                                                                                                                gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACACCGCAATAGCCTGATTTCGCGTGCTTTTGTCGGTCGGACAAGGCGTTTACGCCGCACCCGACATCaatgcctgatgcgatccttgccgcgtcttatcca       <  1:51276‑M2/289‑35 (MQ=255)                                                                                                                                                                                                                                                                                       cGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAAGCCGCATCCGACCTCaatgcctgatgcgccccctgccgcgccttatcaggccta  <  1:1330905‑M2/287‑40 (MQ=255)                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                         CGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTA  >  NC_000913/4295870‑4296434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑