Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	2,765,454	(TATGGCAC)6→5	3.2%	intergenic (‑303/+50)	yfjL ← / ← yfjM	CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein/CP4‑57 prophage, uncharacterized protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2765418 =	99 (0.670)	3 (0.020)	3/410	NT	3.2%	intergenic (‑267/+93)	yfjL/yfjM	CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein/CP4‑57 prophage, uncharacterized protein
	?	NC_000913	= 2765409	99 (0.810)					intergenic (‑258/+102)	yfjL/yfjM	CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein/CP4‑57 prophage, uncharacterized protein

TAACCTCTTCCTGATCTCACCAAGCAGCAACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2765748‑2765418 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtgccatagtgccatagtgccatagtgccatagtgccatagtgcGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAACGATTCAGGCGGAGTAGCC  <  NC_000913/2765409‑2765122                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             TAACCTCTTCCTGATCTCACCAAGCAGCAACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGAAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGG                                                                                                                                                                                                                                                                                                                                             <  1:138182/288‑1   ACCGCTTCCTGATCTGCCCAAGCAGCAACTCGATACTTAGTAATGATTGGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGCCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGC                                                                                                                                                                                                                                                                                                                                          <  2:404782/289‑1     CTCTTCCTGATCTCACCAAGACGCAACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGGGCTATAAGTAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGACCACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCA                                                                                                                                                                                                                                                                                                                                        <  1:925151/289‑1         TCCTGATCTCACCAAGCAGCAACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATA                                                                                                                                                                                                                                                                                                                                      >  2:1311339/1‑287               TCTCACCAAGCAGCAACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATA                                                                                                                                                                                                                                                                                                                              >  1:478419/1‑289               TCTCACCAAGCAGCAACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATA                                                                                                                                                                                                                                                                                                                              >  1:413042/1‑289                  CACCAAGCAGCAACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTG                                                                                                                                                                                                                                                                                                                           <  2:1227733/289‑1                  CACCAAGCAGCAACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACACGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGT                                                                                                                                                                                                                                                                                                                            >  1:1125182/1‑288                   ACCAAGCAGCAACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTG                                                                                                                                                                                                                                                                                                                           >  2:225220/1‑288                     CAAGCAACAACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCGGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCA                                                                                                                                                                                                                                                                                                                        <  1:69915/289‑1                        GCAGCAACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAG                                                                                                                                                                                                                                                                                                                     >  2:237764/1‑289                             AACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCA                                                                                                                                                                                                                                                                                                                <  2:1099607/289‑1                             AACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGC                                                                                                                                                                                                                                                                                                                  <  2:1125182/287‑1                                     ACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCCA                                                                                                                                                                                                                                                                                                        <  2:749224/289‑1                                       TTAGTAATTATTCGCCAGCAGAAAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCAAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCCATA                                                                                                                                                                                                                                                                                                      <  2:971821/289‑1                                         AGTAATTATTCGCCAGCAGACAAGAAAAAATTCTCCGTCGCAACAGAACGAGCAGCGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGACCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGAAACTGTGTCTTATAATATCAAGTGTGATTACAATCGCTGGTGGTGTCATAGTGCCATAGTCCTATAGTGCCATAGCGCCATAtt                                                                                                                                                                                                                                                                                                    >  1:782975/1‑287                                         AGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCCATAGT                                                                                                                                                                                                                                                                                                    >  2:389887/1‑289                                                ATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCA                                                                                                                                                                                                                                                                                                                        <  2:1254514/262‑1                                                ATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCA                                                                                                                                                                                                                                                                                                                        >  1:1254514/1‑262                                                ATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCA                                                                                                                                                                                                                                                                                                                                >  2:881714/1‑254                                                ATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCA                                                                                                                                                                                                                                                                                                                                <  1:881714/254‑1                                                                                              ggagggagCAAAACAGGCCATCAAGATGAGCTATAAGGGGTGGGGGCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACCCGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCATCCAGGAAATAATGCGGGCAACTGTGTTTTATAATATCAAGTGTGGTTACAATCGCTGGCGGTGCCATAGGGCCATAGTGCCATAGTGGCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGG                                                                                                                                                                                                                                               <  1:535480/281‑1                                                                                                    CCCAAAACAAGCCATCATGAGGAGCTATAAGTGGTGGGGTCTGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCA                                                                                                                                                                                                                                                                                                                <  1:1231259/218‑1                                                                                                              GCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACG                                                                                                                                                                                                                               >  2:173614/1‑289                                                                                                              GCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCCATA                                                                                                                                                                                                                                                                                                      >  2:1231259/1‑218                                                                                                                        TGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGG                                                                                                                                                                                                                     <  1:173614/289‑1                                                                                                                                      TGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCA                                                                                                                                                                                                                                                                                                                <  2:162788/184‑1                                                                                                                                      TGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCA                                                                                                                                                                                                                                                                                                                >  1:162788/1‑184                                                                                                                                         GGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTATCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGC                                                                                                                                                                                                                                                                                                                                          >  2:544078/1‑155                                                                                                                                         GGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGC                                                                                                                                                                                                                                                                                                                                          <  1:544078/155‑1                                                                                                                                                                                                                                     CAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCCA                                                                                                                                                                                                                                                                                                        >  1:1089438/1‑97                                                                                                                                                                                                                                     CAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCCA                                                                                                                                                                                                                                                                                                        <  2:1089438/97‑1                                                                                                                                                                                                                                                                                                 TGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCGGTTGGC                                                                                                                                                                                                                                                                                           >  1:839032/1‑50                                                                                                                                                                                                                                                                                                 TGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCGGTTGGC                                                                                                                                                                                                                                                                                           <  2:839032/50‑1                                                                                                                                                                                                                                                                                                     ATAGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGG                                        >  2:464089/1‑289                                                                                                                                                                                                                                                                                                      TAGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTTGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATACCTTGTGATGATGCAGTGATCCGGTTGGACTGTGCCATTACATCTCCTTAATTTTCGCAAAATTGTatcggt                                       >  1:700130/1‑283                                                                                                                                                                                                                                                                                                      TAGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGA                                       >  1:690909/1‑289                                                                                                                                                                                                                                                                                                      TAGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGA                                       <  2:672395/289‑1                                                                                                                                                                                                                                                                                                      TAGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGA                                       >  1:935489/1‑289                                                                                                                                                                                                                                                                                                           CCATAGTGCCATAGTGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTC                                                                                                                                                                                        >  2:756020/1‑139                                                                                                                                                                                                                                                                                                           CCATAGTGCCATAGTGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTC                                                                                                                                                                                        <  1:756020/139‑1                                                                                                                                                                                                                                                                                                           CCATAGTGCCATAGTGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGAAGTCAGGGTTAAAATATTTGTGGAATCCTGAAAAGCTTGATACCTTCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATCGTCAAGGATGTCT                                  >  1:291564/1‑289                                                                                                                                                                                                                                                                                                           CCATAGTGCCATAGTGCCATAGTGCCATAGTGCGGTTGGAGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGAAGTATGTTATATACCCAAATTAGAGGTAAGGGTTACAAAAATTGTGGAATCCTGTAAAGCTTGATACCTGCGACCGAGAATGGCTAAGAAGCATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATTTCCTTACTTTTCGAAAAATTGCCACGGATGTCT                                  >  1:415567/1‑289                                                                                                                                                                                                                                                                                                             ATAGTGCCATAGTGCCATATTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAGTATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAA                                >  1:695137/1‑289                                                                                                                                                                                                                                                                                                                   CCATAGTGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACT                                                                                <  2:196898/235‑1                                                                                                                                                                                                                                                                                                                   CCATAGTGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACT                                                                                >  1:196898/1‑235                                                                                                                                                                                                                                                                                                                   CCATAGTGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGAGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATCTTTGTGGAATTCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCAGTGGTGATGATGCAGTGATACGATTGGACTGTGTTATACCATCTCCTTAATTTTCGCAAAATTCTCACGGATTTCTAAATGGAt                          >  1:217405/1‑288                                                                                                                                                                                                                                                                                                                         TGCCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAAC                    >  2:987220/1‑289                                                                                                                                                                                                                                                                                                                           CCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAACGA                  >  2:1296732/1‑289                                                                                                                                                                                                                                                                                                                           CCATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAACGA                  >  1:182557/1‑289                                                                                                                                                                                                                                                                                                                             ATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAACGATT                >  2:975065/1‑289                                                                                                                                                                                                                                                                                                                             ATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGA                                                                                  <  1:967627/223‑1                                                                                                                                                                                                                                                                                                                             ATAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGA                                                                                  >  2:967627/1‑223                                                                                                                                                                                                                                                                                                                              TAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAACGATTC               >  2:551988/1‑289                                                                                                                                                                                                                                                                                                                              TAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGG                                                                                                          <  1:797398/198‑1                                                                                                                                                                                                                                                                                                                              TAGTGCCATAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGG                                                                                                          >  2:797398/1‑198                                                                                                                                                                                                                                                                                                                                      TAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAACGATTCAGGCGGAG       >  2:188433/1‑289                                                                                                                                                                                                                                                                                                                                      TAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGA                                                                                            <  2:1312766/204‑1                                                                                                                                                                                                                                                                                                                                      TAGTGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGA                                                                                            >  1:1312766/1‑204                                                                                                                                                                                                                                                                                                                                         TGCGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAACGATTCAGGCGGAGTA     >  1:288130/1‑288                                                                                                                                                                                                                                                                                                                                           CGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTATACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAACGATTCAGGCGGAGTAGCC  >  2:248967/1‑289                                                                                                                                                                                                                                                                                                                                           CGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAACGATTCAGGCGGAGTAGCC  >  1:454231/1‑289                                                                                                                                                                                                                                                                                                                                           CGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAACGATTCAGGCGGAGTAGCC  >  2:1046053/1‑289                                                                                                                                                                                                                                                                                                                                           CGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAACGATTCAGGCGGAGTAGCC  >  2:1065959/1‑289                                                                                                                                                                                                                                                                                                                                           CGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAACGATTCAGGCGGAGTAGCC  >  1:1138852/1‑289                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             TAACCTCTTCCTGATCTCACCAAGCAGCAACTCGATACTTAGTAATTATTCGCCAGCAGACAAGAAAAAATTCTACGTCGAAACAGAACGAGCAGAGAGCCCAAAACAAGCCATCATGATGAGCTATAAGGAGTGGGGTCCGGATGGGCAAGGCATCAACAATATAGAAAGCCATGAACACGCGATGTTGACATTGCTCAAAGAGCATCGAGATATGACCCTACCAACCAGGAAATAATGCGTGCAACTGTGTTTTATAATATCAAGTGTGATTACAATCGCTGGCGGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGCCATAGTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2765748‑2765418 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gtgccatagtgccatagtgccatagtgccatagtgccatagtgcGGTTGGCGGTCTCAGCTCTGAACTATTTGAAAACCAAAACCTTGATTAAGGCTGTGCCTACCTTACGTTAGTTGGGGCAGTATGTTATATAACCAAATTAGACGTCAGGGTTACAATATTTGTGGAATCCTGAAAAGCTTGATACCTGCGACCGAGAATGGCTGTGAAGTATTTAGATCACTGGTGATGATGCAGTGATCCGATTGGACTGTGTCATAACATCTCCTTAATTTTCGCAAAATTGTCACGGATGTCTAAATGGAGTCGAACGATTCAGGCGGAGTAGCC  <  NC_000913/2765409‑2765122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.