Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,311,197	G→T	17.8%	E37* (GAA→TAA)	tonB →	membrane spanning protein in TonB‑ExbB‑ExbD transport complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,311,197	0	G	T	17.8%	191.4 / 9.0	77	E37* (GAA→TAA)	tonB	membrane spanning protein in TonB‑ExbB‑ExbD transport complex
Reads supporting (aligned to +/- strand):  ref base G (25/36);  new base T (12/3);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 9.09e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCC  >  NC_000913/1310911‑1311485                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                        aaGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGaa                                                                                                                                                                                                                                                                                                       <  2:231165/289‑1 (MQ=255) aaGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGaa                                                                                                                                                                                                                                                                                                       <  2:42737/289‑1 (MQ=255) aaGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGCGCTG‑ATT‑TAT‑AC‑CTCGGTACATCAGGGTATTgca                                                                                                                                                                                                                                                                                                       >  2:528041/1‑287 (MQ=255)               gCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTCTTTT‑AC‑CT‑GGTACATCCGGTTATTTTAATAactgggcctg                                                                                                                                                                                                                                                                                          >  1:1214932/1‑288 (MQ=255)                 aaCGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTAcctgcgcctgcg                                                                                                                                                                                                                                                                                        <  2:1214932/288‑1 (MQ=255)                                               tGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTT‑TTT‑AC‑CCCGGGACAATTGGTTCTTTAAATAACTTCGCCTTCGCatcgccattctctccagactgtttcgcac                                                                                                                                                                                                                                                          >  2:1387146/1‑260 (MQ=255)                                               tGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCctg                                                                                                                                                                                                                                                        <  2:956925/289‑1 (MQ=255)                                               tGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCATTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGGCTG‑CTC‑TTT‑AC‑CCCCGTAAACCAGGTTATTTAACTTCCTGCGCCTGTGCAGCCGCTTTTTGTTACGATGGGT‑ATGCct                                                                                                                                                                                                                                                         >  1:600765/1‑288 (MQ=255)                                                  aTAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCctgct                                                                                                                                                                                                                                                      <  2:1003328/288‑1 (MQ=255)                                                          aGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTT‑CTC‑TAT‑AC‑CCCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCCATTTCTGTCACGATGGTT‑TCCGctgctggttccga                                                                                                                                                                                                                                              >  2:824788/1‑281 (MQ=255)                                                          aGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGaa                                                                                                                                                                                                                                             <  2:1278673/289‑1 (MQ=255)                                                           gggTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTGTG‑GTC‑TTT‑AC‑CCCGGGACATCAGGGTATTCACATTCCTTCGCCTGCCCCGCCGACTTCTGTCACCAGGTGT‑ACCCCTGCTGAtccccaa                                                                                                                                                                                                                                             >  1:1230989/1‑283 (MQ=255)                                                           gggTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACATGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTGTT‑CCT‑TTT‑TC‑CCCTGTTCATCAGTTTTTTTTAATCTCTGCGCGTGCGTCGCCGTTTTCCTTTACCAGGGTT‑ATGCCTGTTGTtctccga                                                                                                                                                                                                                                             >  1:1003328/1‑285 (MQ=255)                                                                aTTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTTAACTACCTGCGCCCGCGCAGCCGATTTTTGTCTCCGTGGTT‑TCGCCTGCTGTTTTCGAACcc‑cc                                                                                                                                                                                                                                       >  1:158958/1‑288 (MQ=255)                                                                aTTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑cc                                                                                                                                                                                                                                       >  2:506346/1‑288 (MQ=255)                                                                      ccAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGCGACCTGGTTTTTCTACTGAAATGATTATGACTTCCATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTGTT‑CCC‑TCC‑TA‑CACGTCTCATCACGGTGTTGATAGACCTCCGCCTGCGCAGCCGCCTTCTGTCTCAATTGTT‑CCgacgtctgttccctgaccaccccaacg                                                                                                                                                                                                                                  >  1:989225/1‑262 (MQ=255)                                                                      ccAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTTCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGc                                                                                                                                                                                                                                 >  2:285988/1‑288 (MQ=255)                                                                             tGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACACCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAgc                                                                                                                                                                                                                         <  1:848492/289‑1 (MQ=255)                                                                                       aCTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGaa                                                                                                                                                                                                                                                                                                       <  2:589467/203‑1 (MQ=255)                                                                                       aCTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGa                                                                                                                                                                                                               <  1:506346/289‑1 (MQ=255)                                                                                       aCTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGa                                                                                                                                                                                                               <  2:1075544/289‑1 (MQ=255)                                                                                         ttatcctgatcGTCTTGCCTTTTGTAATATGATTGCTATTGGCATTTAAAATCGGGGCCTGGTTTTTCTACTGAAATGTTTAGGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGc                                                                                                                                                                                                             <  1:74219/287‑1 (MQ=255)                                                                                             cctgatcGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGaa                                                                                                                                                                                                                                             <  2:346524/254‑1 (MQ=255)                                                                                             cctgatcGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGa                                                                                                                                                                                                               <  2:1426713/283‑1 (MQ=255)                                                                                                gatcGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCggtggt                                                                                                                                                                                                      <  1:353475/289‑1 (MQ=255)                                                                                                gatcGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCggtggt                                                                                                                                                                                                      <  2:158958/289‑1 (MQ=255)                                                                                                     tCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CCCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCAc                                                                                                                                                                                                                                                                       <  2:1007876/221‑1 (MQ=255)                                                                                                       ttGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGGGTG‑CCC‑CAT‑AC‑TCCCGTACATTCGGCTATTTAACGAACCCCGCCCGCCCCTCCCACTTCTGTCACCAATGTT‑CAACCTCGCGATCGCGAACCG‑CCNCACGCCGCCCCGCCGCCAc                                                                                                                                                                                                                   >  1:1119836/1‑269 (MQ=255)                                                                                                       ttGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGGCTG‑CTC‑T‑T‑ACACCCTGGACAACCAGTTGTTGTACTCCCTGCGGCTGCGCCGCCGACTTCTCTCACGATGGTT‑ACGTTTGGCTATCTTGACACC‑CCCCCAGCCCTTCCGCACCCCCCGGCGCCGGGGGTACAG‑ctg                                                                                                                                                                                              >  1:1426713/1‑287 (MQ=255)                                                                                                            ttATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAAcc                                                                                                                                                                                         <  1:285988/289‑1 (MQ=255)                                                                                                              aTTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CGC‑TAT‑AC‑CCCTGTACATCAGGTTATTTAACTACCCGCGCCTGCGCAGCCGACTTCTTTCACGATGTTT‑TCCCCTGCTTGTATCGAACCG‑CCACCAGCCGTTCAGCCCCCCCCAGAGGCGGCGGTAGGG‑CCAGCACCgg                                                                                                                                                                                       >  1:1170736/1‑289 (MQ=255)                                                                                                              aTTGAATATGATTGATATTTGCATTTAAAATCTGGACCTGGTTTTTCTACGGAAATGATTATGACTTAAATTACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCTGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑GTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCgg                                                                                                                                                                                       <  1:1328540/289‑1 (MQ=255)                                                                                                                    tatGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGaa                                                                                                                                                                                                                                                                                                       <  2:670931/174‑1 (MQ=255)                                                                                                                    tatGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGGTATTGaa                                                                                                                                                                                                                                                                                                       >  1:670931/1‑174 (MQ=255)                                                                                                                         ttGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGGCTG‑CCG‑TCT‑AA‑CTCCGAACAACCGGTTATTTTACATACTGCTCCTCCCCGCCCCATTTTTGTCATGCTGGGT‑AGCCCTACTGAGACCTAACCG‑CCGAAAGCCGGCCGGCCGCCACACGACCCGGTGGTTGGG‑ccctgctcgcaacatcaacc                                                                                                                                                                             >  1:847880/1‑270 (MQ=255)                                                                                                                                       tAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGcc                                                                                                                                                              <  2:1371340/289‑1 (MQ=255)                                                                                                                                          aaTCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCaaa                                                                                                                                                           <  2:1230989/289‑1 (MQ=255)                                                                                                                                             cGAGACCTGGTTTTTCTACTGAAATGATTATGCCTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGTTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAaaga                                                                                                                                                        <  2:600765/289‑1 (MQ=255)                                                                                                                                                gaCCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTTAACTACCTGCGCCTGCGCCGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGGTCAGCCGCCACCCGGGCCGGTGGTAGAG‑CCAGAACCGGAACCCGAACCGGACCCCCAACCCCCCAaagaaa                                                                                                                                                      >  2:378131/1‑287 (MQ=255)                                                                                                                                                                      caTTATGACTTCAATGCCCCTTGATTTACCTGGCCGCTTCCCCTGGCCGCAGTTACTTTGGTTCTGCATTCCTGGTGCTGTTGTGGCGGTTCTG‑CTC‑TAT‑AC‑TTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTGCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCTAACCGCCAAAAGAAGCACCGGTTGTCATTGAAaag‑ccg                                                                                                                              <  1:173608/288‑1 (MQ=255)                                                                                                                                                                              cTTCAATGACCCTTTATTTACCTCGCCGCTTCCCCTGGCCGTCGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaag‑ccgaagcc‑g                                                                                                                       <  2:472869/287‑1 (MQ=255)                                                                                                                                                                                 cAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTGTCCT‑TAT‑CC‑CACGTTA‑ATCAGGGTATTGTACTATCTGCGGCTGCGCAGCCCATTTCTGTCACCAATGTT‑ACCCCTGATGATCTCGAACCC‑CCCCAAACCCGTCACACCACCCCGGGGCCGGTGGGCGAG‑CCAGAACCCGAAGCTGGGACGAACCCCCAAACCCCAAACCAAGCACAGCGGGTCACTTAACAG‑GCCAAACC‑Gaaaa                                                                                                                   >  1:472869/1‑287 (MQ=255)                                                                                                                                                                                        ccTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTCCTTTCGGTCTGCATTCATGGTGCTTTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAaag‑ccg                                                                                                                              <  2:952650/271‑1 (MQ=255)                                                                                                                                                                                          ttGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCggtggt                                                                                                                                                                                                      <  2:876004/199‑1 (MQ=255)                                                                                                                                                                                             gattACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TCTAAC‑CCCGGT‑CATTCGGTTTTTGAACTACCTGCGCCCGCCCCGCCGATTTTTTTCACCATGGTT‑TCGCCCGCTGATCCCGAACCC‑CCCCCAACCCTTCCGCCGCCCCCCGGGCCGGGGGGAGCGCCCAGAACGGGAACCTGGCCCAACCCCCCACCCCCCCAAAAAAGACCCGGGGGGCTTTTaaaacacaagcacaacaccaaacacaaaac                                                                                                         >  1:174990/3‑263 (MQ=255)                                                                                                                                                                                                  ccTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑GTC‑TTT‑AC‑CTCGGTAAATCAGGGGTTTTAAATACCTTTCGCTGCGCCGCCGATTTTTTTTAACATTGTT‑ACGCCTGCTGAACTCCAACCC‑CCCCAAACCGGTCAGCCGCCCCCGGAGCCGGGGGGTGAA‑GCAAAAACGGGACCCGGGGCGCACCCCCGACCGCCAAAAGAAACACCGGGGGGCATTGAAAAG‑CCGAAGCCGGAAACCtttctcctataacac                                                                                                   >  1:952650/1‑274 (MQ=255)                                                                                                                                                                                                  ccTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TTT‑CC‑CCCGGTCCATCAGGGTATTGAACTACCTGCGCCTGCGCAGCCCATTTCTTTCACGATGGTT‑ACGCCTGCCGATCTCCAAACG‑CCCCAAACCGTTCCGCCGCCCACGGGGCCGGGGGGAGGG‑GCAGAACCCGAACCTGCGCCGCACCCCCACACGCCCAAAAAAGAACCCGTGGGCATCGAAAAA‑CCGAAGCC‑CAAACCCAATGCAAAACCaaaaa                                                                                                 >  1:354402/1‑287 (MQ=255)                                                                                                                                                                                                  ccTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CCCGGTACATCCGGTTATTTAACTACCTGCGCCCGCGCCGCCGATTTTTGTCCCGATGGTTCACACC‑GCTGATCTCTCACCG‑CCACAAGCCGTTCAGCCGCCCCCGGCGCCGGGGGGAGAG‑ACAGAACCCGAACCTGAGCCCATCCCCCAACCCCCCAAAGAAGCACCGGGGGGCATAGAAAAA‑CCCGACAC‑CAAACCAAAACCCAAACCgcac                                                                                                  >  1:11244/1‑285 (MQ=255)                                                                                                                                                                                                         gcTTCCCCGGGCCTACGTTCCTTTCGGTTTGCATTCATGGTGCTGTTGTGGCGGGTCGG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGaa                                                                                          <  2:1349410/289‑1 (MQ=255)                                                                                                                                                                                                                    cgcaCGTTACTTCTGGTTTGCAGTCATGGTGTTGTGGTGGCGGGTCTC‑CGC‑TAT‑AC‑CTCGTTACATGAGGTTATTGAACTGCTCGCGGCTACGCAGCCGATTGCTGTCACGAGCGTT‑ACGCCTGCGGAGCGCAAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGGG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAgg                                                                               <  1:777851/286‑1 (MQ=255)                                                                                                                                                                                                                            aCTGTCTGTCTGCATTCTGGGTGCTGTTGTGGCGGGTCTG‑TTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑TCGCCTGCTGTTCTCAAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGTTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCa                                                                       <  2:144512/289‑1 (MQ=255)                                                                                                                                                                                                                                   gTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑GTA‑TTT‑TC‑CACGGTACATTCGGGTATTTAACTAACTACGGCCGCCGCGCCGATTTCTGTCCCGATTGGT‑ACGCCTGCTGGTCTCCACCCGCCCACACCCCG‑TCAGCCCCCACCGGAGCCGGGGGTTGGG‑CCCGCACCGGAACCTGGACCGACACCCGCAACGCCACAAGAAGCACCGGCGGGACTTTACAAGACTGAACCC‑GACCCCCAACCCAAAACC‑ACAACGGGGAAAAAAGTACAAGAACCGCCACAAAcg                                                                 >  1:934483/1‑288 (MQ=255)                                                                                                                                                                                                                                       gCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGGTATTGAAATACCTGCGCCTGCGCCGCCGATTTCTGTCACGCTGGTT‑ACGCCTGCTGATCTCGAACCG‑CCCCAAACCGTTCAGCCGCCCCCCGAGCCGGTGGTAGAG‑CCAGAACCGGAAACCGAGCCGAACCCCGCAACGCCCAAAGAAGCACCCGGGGGACGTGAAAAA‑CCGAAGCC‑GCAACCCAAACCCAAACCAAAACCCGGGGAAAAGGGACAGGAGAAGCAC‑AAAAGCG‑acag                                                          >  1:744001/1‑286 (MQ=255)                                                                                                                                                                                                                                       gCATTAATGGTGCTGTTTTGGCGGGTCCT‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTTAACTACCTGCCCCTCCGCAGCCGATTTCTGTCACGTTGGTT‑ACGCCTGCTGATCCCGAACCG‑CCACAGGCCGTTCAGCCTCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGt                                                          <  2:1174485/289‑1 (MQ=255)                                                                                                                                                                                                                                        gcaggggccgatgtggataagggacatG‑CTT‑TAT‑AC‑TTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCCCCCTATTTCGGTCCCGATGGTT‑ACGCCTGCTGTTCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGCTCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAACCCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGt                                                          <  1:782700/262‑1 (MQ=255)                                                                                                                                                                                                                                          ttCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCaa                                                       <  2:174990/289‑1 (MQ=255)                                                                                                                                                                                                                                                          ggCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTcgcg                                        <  2:848097/288‑1 (MQ=255)                                                                                                                                                                                                                                                            ggggCCTG‑CTC‑TTT‑AC‑CTCGGTACCTCAGGTTATTTACTTCCATGCTCCTACGCACCCTATTTCTGTCACGATGGTT‑ACGCCTCCTGTTCTCGACCCG‑CCACAAGCCGGTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTCGCGTc                                      <  1:1138085/287‑1 (MQ=255)                                                                                                                                                                                                                                                            agggTCTG‑CTG‑TCT‑CC‑CTGGTTACTTCAGTTGATTGAACTACCTGAGCCTGCGCAGCCGATTGCTGTCACGTTGGTT‑ACGCCTGCTGATCCCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTCGCGTc                                      <  1:893991/287‑1 (MQ=255)                                                                                                                                                                                                                                                                           t‑aC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCATGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGAAGCCA‑AAACGCG‑ATGTAAAAACCGGAGAGTCGCGTCCGGCATTACCGttt                        >  1:824927/1‑289 (MQ=255)                                                                                                                                                                                                                                                                           t‑aC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTGCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGttt                        <  2:617333/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                cTCGGTACATCAGGTTATTTAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGaa                     <  2:824927/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                  cGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAg                                                                                                  >  1:424109/1‑212 (MQ=255)                                                                                                                                                                                                                                                                                  cGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAg                                                                                                  <  2:424109/212‑1 (MQ=255)                                                                                                                                                                                                                                                                                  cGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCCGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTCCCGTCCGGACTACCCGTTTGaaaa                   >  1:312387/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                  cGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGaaaa                   >  2:601658/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                  cGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGaaaa                   >  2:475779/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                  cGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGaaaa                   >  2:1294864/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                  cGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAAAGTCGCGTCCGGCATCACCGTTTGaaaa                   >  2:239203/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                  cGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCAA‑AAAAGAG‑ATGTCAAACCCGTAGAGTCGCGTCTGGCATCACCGTTTGaaaa                   >  2:163310/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                  cGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGACGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTa                                                >  2:782700/1‑260 (MQ=255)                                                                                                                                                                                                                                                                                  cGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAAACGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGACAAACCCGTAGAGTCGCGTAAGGCATCACCGGTTGaaaa                   >  2:1208076/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                  cGGTACAACAGGTTATTGACCTACCTGCGCCTGCGAAGCCGATTTCTGTCACGATAGTT‑ACGCCTGCTGATCTCGCACCG‑CCACAAGCCGTTCCGCCGCAACCGGAGCCGGCGGTAGAG‑CCAGACCCGGAACCTGAGCCGATCACCGAACCGCCAAATGAAGCACCGGAGGTCACTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGCGCAGACA‑AAACGCG‑ACGTCAAACCCGTGTAATCGCGACCGGCCGAAACGTTGGaaaa                   >  1:719424/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                     tACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATAc                >  1:953054/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                       cATCAGGTTATTGAACTACCTGCGCCTGCGAAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGACACCGGAGCCCGTGGTATAG‑ACAGAACCGGAACCTGAGCCGATACCCTAACCGCCACAAGAAGCACCGGCGGCCATTTAAAAG‑CCGAAGCC‑GAACCCTAAGCCAAAACAGAAGCGGGTGAAAAAGGTACAGGAGCAGCAA‑AAACGCGCATG‑CAAACCCGGAGAGTCGCATCCGGccccccactttcaacatacg               >  1:763539/1‑269 (MQ=255)                                                                                                                                                                                                                                                                                               tATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGGCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATAcggcaccggc       >  2:433197/1‑288 (MQ=255)                                                                                                                                                                                                                                                                                               tATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCAGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAAGCTGAGCCGATCCCCGAACCGCCAACAGAAGCCCCGGCGGTAATTGAAAAG‑CAGAAGCC‑GACACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGAAACGGAGAAGACA‑ACACGGG‑ATCTGAAACCCGGAGAGGCGAGTACGGCAACACCGTTTGAAAACTaggcaccggca      >  1:1388917/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                                  tGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGc   <  2:920737/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   gAACTACCTGCGCCGGCGCACCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGcc  <  2:744001/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                        AAGGGCGAAGATCTGCAACGGAAAGATGATGTCTTTGTTAAGGCCATGCATAAAGTAAGGGTAATTACGCCAAAAATGACATTTTCACTGATCCTGATCGTCTTGCCTTATTGAATATGATTGCTATTTGCATTTAAAATCGAGACCTGGTTTTTCTACTGAAATGATTATGACTTCAATGACCCTTGATTTACCTCGCCGCTTCCCCTGGCCGACGTTACTTTCGGTCTGCATTCATGGTGCTGTTGTGGCGGGTCTG‑CTC‑TAT‑AC‑CTCGGTACATCAGGTTATTGAACTACCTGCGCCTGCGCAGCCGATTTCTGTCACGATGGTT‑ACGCCTGCTGATCTCGAACCG‑CCACAAGCCGTTCAGCCGCCACCGGAGCCGGTGGTAGAG‑CCAGAACCGGAACCTGAGCCGATCCCCGAACCGCCAAAAGAAGCACCGGTGGTCATTGAAAAG‑CCGAAGCC‑GAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCA‑AAACGCG‑ATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCC  >  NC_000913/1310911‑1311485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑