Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,527,589 C→T 60.6% Q375* (CAG→TAG)  yrfF → putative RcsCDB‑response attenuator, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,527,5890CT60.6% 161.3 / 217.8 201Q375* (CAG→TAG) yrfFputative RcsCDB‑response attenuator, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (46/33);  new base T (64/57);  total (111/90)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

Only 100 of 208 total aligned reads displayed.

TATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTG  >  NC_000913/3527306‑3527874
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                             
tatTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGAcc                                                                                                                                                                                                                                                                                          >  1:150212/1‑289 (MQ=255)
       cTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCGGCTGAAATTCACTTTCTGATGGATGAAAGGCGCGTAGTCCATTTaa                                                                                                                                                                                                                                                                                    >  1:284957/1‑288 (MQ=255)
          ggTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGcc                                                                                                                                                                                                                                                                                 >  2:1371139/1‑288 (MQ=255)
            tattttCGACCTGGTCAAGCCCGCACACTTGCAGCCATACATTGCTCATGATCTCAGTCAACAAACCGATATCGATATCTATCTCGACCTCCATGTAGTGCGTCAGGGACGATAGCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGccac                                                                                                                                                                                                                                                                               <  1:555724/288‑1 (MQ=255)
            tattatCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGccac                                                                                                                                                                                                                                                                               <  2:150212/288‑1 (MQ=255)
               tatCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGc                                                                                                                                                                                                                                                                           >  2:133784/1‑289 (MQ=255)
                      cTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCATTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCAACAAGCGTAAaac                                                                                                                                                                                                                                                                    >  2:1117524/1‑289 (MQ=255)
                      cTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAaac                                                                                                                                                                                                                                                                    >  1:429112/1‑289 (MQ=255)
                      cTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTAACTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCCTGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCCGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGCTATGCCGCGGAAATTCTCTCTCTCATGGATGAAAGTCGCGCATACCATTCAAGCGACCAGCGGACaac                                                                                                                                                                                                                                                                    >  1:1219137/1‑289 (MQ=255)
                         gTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAaaca                                                                                                                                                                                                                                                                   >  2:133222/1‑287 (MQ=255)
                             aTCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCAAGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGc                                                                                                                                                                                                                                                             <  2:747935/289‑1 (MQ=255)
                                        tGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAGATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGccac                                                                                                                                                                                                                                                                               <  1:1050191/260‑1 (MQ=255)
                                        tGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCg                                                                                                                                                                                                                                                  >  1:171984/1‑289 (MQ=255)
                                                  aCATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGCTTATGCTGTTATTCTGGATCCTGCTGGATATGCTGCTGAACGTAATTCTCTCATGGATGAAAGGCGCGTAGCACATGCAAGCCACCAGCGTAAAACAACTGGCCGACGCTGGCGTGCGGGTAg                                                                                                                                                                                                                                         >  1:1063692/1‑288 (MQ=255)
                                                        cTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGt                                                                                                                                                                                                                                           >  2:1136276/1‑280 (MQ=255)
                                                        cTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATAc                                                                                                                                                                                                                                  >  1:128205/1‑289 (MQ=255)
                                                        cTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATAc                                                                                                                                                                                                                                  >  2:387047/1‑289 (MQ=255)
                                                        cTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATAc                                                                                                                                                                                                                                  >  2:547441/1‑289 (MQ=255)
                                                        cTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATAc                                                                                                                                                                                                                                  >  2:1250127/1‑289 (MQ=255)
                                                        cTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGGGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCCCTCATGGATGAAAGGCGCGAAGACCCTTGCAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGGGGGTAGGAGAtcc                                                                                                                                                                                                                                  >  1:718792/1‑287 (MQ=255)
                                                        cTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGATGATGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCAACAGCGTAAAAAAACTGGCTGACGCTGGCGTGCGGGTGAGCTATAc                                                                                                                                                                                                                                  >  1:881022/1‑289 (MQ=255)
                                                        cTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTCTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGCAAAACAACTGGCTGACGCTGGAGTGCGGGTAGGCgcaaa                                                                                                                                                                                                                                  >  1:611087/1‑285 (MQ=255)
                                                         tCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACa                                                                                                                                                                                                                                 <  1:1059050/289‑1 (MQ=255)
                                                           aGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCAGCATGATAAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGACAGGCGCGTAGACCATTTAAGCCCCCAGCGTAAAACAACTGGCTGACGCTGGCGTTCGGGGAGGCGCTACAtt                                                                                                                                                                                                                               >  2:1310653/1‑289 (MQ=255)
                                                             gATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCgg                                                                                                                                                                                                                                             <  2:1038246/273‑1 (MQ=255)
                                                                ctcGGTCAACAAACCGATATCGATATCTATCTCGACCGGCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAGCTTTCCGTTAAAGCACTGGCTGCACAGAACGATTATCGCTGCGGTCTCGCTACTGGTGCTGTTTATGCTGTTATTTAGGCTCCTGCTGGATCTGCCGCTGAAATTCACTCTCCCATGGATGAAAGGCGCGTAGACGAATGAAGCCACCAACGTTAAACAACTGGCTTCCGCTGGCTTGCGAGTAGGCGACACAGTGctca                                                                                                                                                                                                                          >  1:510811/1‑286 (MQ=255)
                                                                  cGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGc                                                                                                                                                                                                                                                             >  2:325013/1‑252 (MQ=255)
                                                                  cGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAaacaa                                                                                                                                                                                                                                                                  <  1:202458/247‑1 (MQ=255)
                                                                     tCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTGAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGCGCGGGTAGGCGATACATTGCGTATTAg                                                                                                                                                                                                                      <  2:443597/288‑1 (MQ=255)
                                                                     tCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGATAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAg                                                                                                                                                                                                                      <  1:461696/288‑1 (MQ=255)
                                                                     tCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAg                                                                                                                                                                                                                      <  2:943301/288‑1 (MQ=255)
                                                                     tCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAg                                                                                                                                                                                                                      <  2:535792/288‑1 (MQ=255)
                                                                     tCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGGAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGAGCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAg                                                                                                                                                                                                                      <  1:1031789/288‑1 (MQ=255)
                                                                       aacaaACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATTCCGCTGAAATTCCCTCTCTCATGGATGACAGGCGCGCAGACCATTGAAGCCACCAGCGTAACACAACTGGCTGACGCTGGCGTGAGGGTAGGCGATCCATTCCGTACTAACgg                                                                                                                                                                                                                   >  1:443597/1‑289 (MQ=255)
                                                                              cgatatcgatatCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGa                                                                                                                                                                                                            <  1:1098755/289‑1 (MQ=255)
                                                                              cgatatcgatatCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGa                                                                                                                                                                                                            <  1:536813/289‑1 (MQ=255)
                                                                                 tatcgatctcTATCTCGACCGCCATGTAGTGCGTCATGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCATATTAGCGGTACGGGAAtg                                                                                                                                                                                                         <  2:839415/289‑1 (MQ=255)
                                                                                   tcgatatcTATCTCGACCGCCATGTAGTTCGTCAGGGACCATATCTTTCGCTGCCTGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTAAGATTATCGCTGCGGGCTCGCTGCTGTTGCTGTTTATGCTGTTATTCTGGATCCCGCTTGATCTGCCGCTGCACTTCACTCTCTCATGGTTGAGAGGCACTTCGACCTTTGAAGCCACTAGCGTAAAACAACTGGCTGCCGCTGGCGTTCGGGTAGGCCATACCTTGCGTATTAGCTGTACGGTAAtgtg                                                                                                                                                                                                       >  1:1264417/1‑289 (MQ=255)
                                                                                    cgatatcTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATgtgt                                                                                                                                                                                                      <  1:1037242/289‑1 (MQ=255)
                                                                                      atatcTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAg                                                                                                                                                                                                                      >  2:1231347/1‑271 (MQ=255)
                                                                                                caaCCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGACGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAACGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACt                                                                                                                                                                                          >  2:926616/3‑289 (MQ=255)
                                                                                                 gACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACtt                                                                                                                                                                                         >  2:850623/1‑288 (MQ=255)
                                                                                                 gACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACAATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTCCGGGTAGGCGATACATTGCGCATTAGCGGTACGGGAATGTGTAATATTCGAACtt                                                                                                                                                                                         >  2:1412915/1‑289 (MQ=255)
                                                                                                         tGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGt                                                                                                                                                                                                                                                 <  1:1107916/225‑1 (MQ=255)
                                                                                                                  tCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGcgcg                                                                                                                                                                        >  2:799827/1‑289 (MQ=255)
                                                                                                                        aCGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAg                                                                                                                                                                                                                      >  2:1032509/1‑237 (MQ=255)
                                                                                                                                 ttCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACa                                                                                                                                                                                                                                 <  2:1017745/217‑1 (MQ=255)
                                                                                                                                    gCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGGATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAGACCAATTCACCtttt                                                                                                                                                      >  1:350737/1‑289 (MQ=255)
                                                                                                                                                aGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGAc                                                                                                                                          <  2:869677/289‑1 (MQ=255)
                                                                                                                                                aGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTg                                                                                                                                            >  2:398013/1‑287 (MQ=255)
                                                                                                                                                            tCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGc                                                                                                                                                                                                                                                             <  2:1058697/162‑1 (MQ=255)
                                                                                                                                                                       ccTGGCTACGCAGTACGATTATCGCTGCGGGCTCGCTGTTGGTGCTGTGTATGCTGGTATTCTGGATCCCGCTGGATATGCCGCTGAATTTCACTCTCACAGGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGGGTGAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATGGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAac                                                                                                                     <  1:398013/286‑1 (MQ=255)
                                                                                                                                                                       aCTGGCGTCGCATTACGATTATCGCGTCGGGCTGGCTGCTGGTGCTGTTTATGCGGTTATGCTGGATCCCGCTGGATATGCCACTGAAATTCACTTCCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGATCTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTGTGACTGCTCGCAGATCATCTGGAacga                                                                                                                   <  1:1412915/289‑1 (MQ=255)
                                                                                                                                                                           gCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGc                                                                                                                >  2:110450/1‑288 (MQ=255)
                                                                                                                                                                            cTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAGCGACGcc                                                                                                               <  2:30856/288‑1 (MQ=255)
                                                                                                                                                                               cgcAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACAtt                                                                                                                                                                                                                               <  2:563078/173‑1 (MQ=255)
                                                                                                                                                                                gcAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTAGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCt                                                                                                           >  2:11762/1‑288 (MQ=255)
                                                                                                                                                                                  aGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCa                                                                                                         <  1:1137539/288‑1 (MQ=255)
                                                                                                                                                                                     aCGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAACGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGc                                                                                                     <  2:881022/289‑1 (MQ=255)
                                                                                                                                                                                       ggttATCGCTGCGGGCTCGCTGTTGGTGCTGTTTATGCTGTTAGTCTGGCTCCCGCTGTATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCa                                                                                                   <  2:1272457/287‑1 (MQ=255)
                                                                                                                                                                                       gATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCa                                                                                                   <  2:670196/289‑1 (MQ=255)
                                                                                                                                                                                            tCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTAcc                                                                                              <  2:171984/289‑1 (MQ=255)
                                                                                                                                                                                            tCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTAcc                                                                                              <  1:1371139/289‑1 (MQ=255)
                                                                                                                                                                                              gCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAg                                                                                                                                                                                                                      >  2:1083666/1‑167 (MQ=255)
                                                                                                                                                                                              gCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAg                                                                                                                                                                                                                      <  1:1083666/167‑1 (MQ=255)
                                                                                                                                                                                              gCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGa                                                                                                                                                                                                            <  2:216069/177‑1 (MQ=255)
                                                                                                                                                                                                  cGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTAcc                                                                                              <  2:958200/283‑1 (MQ=255)
                                                                                                                                                                                                  cGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTAcc                                                                                              >  1:958200/1‑283 (MQ=255)
                                                                                                                                                                                                    ggCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCa                                                                                                   <  1:323789/276‑1 (MQ=255)
                                                                                                                                                                                                     gCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTg                                                                                      <  2:611087/288‑1 (MQ=255)
                                                                                                                                                                                                     gCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTg                                                                                      <  1:824580/288‑1 (MQ=255)
                                                                                                                                                                                                                     gTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTAGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCg                                                                     <  1:11762/289‑1 (MQ=255)
                                                                                                                                                                                                                        tATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACg                                                                  >  2:807517/1‑289 (MQ=255)
                                                                                                                                                                                                                           gCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTc                                                                                                                                                             <  2:64019/195‑1 (MQ=255)
                                                                                                                                                                                                                           gCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTc                                                                                                                                                             >  1:64019/1‑195 (MQ=255)
                                                                                                                                                                                                                                tATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTg                                                            >  2:468942/1‑287 (MQ=255)
                                                                                                                                                                                                                                tATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCAt                                                                                                  <  1:1024630/249‑1 (MQ=255)
                                                                                                                                                                                                                                tATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCAt                                                                                                  >  2:1024630/1‑249 (MQ=255)
                                                                                                                                                                                                                                  ttatGGATCCCGCTGGATATGCCGCTGAAAATCACTCTCTCATGGATGAAAGGGGCGTAAACCATGGAAGCCACCAGCGTAAAACAACGGGCTGACGCTGGCGTGTGGGTAGGCGATACATTGCGTATTAGCTGTACTGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGa                                                                    <  1:869040/274‑1 (MQ=255)
                                                                                                                                                                                                                                         tCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGACAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAAACTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCGGCTCATTGCCATTACAAGAATCTGACCTGGCCAACAAAGCGACGGCATTGACTGAAGCAGt                                                 >  2:1143777/1‑289 (MQ=255)
                                                                                                                                                                                                                                         tCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGa                                                                                                                                           >  1:801032/1‑199 (MQ=255)
                                                                                                                                                                                                                                         tCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGa                                                                                                                                           <  2:801032/199‑1 (MQ=255)
                                                                                                                                                                                                                                         tCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGt                                                 >  2:1427740/1‑289 (MQ=255)
                                                                                                                                                                                                                                         tCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAAGCGCGTAGACCATTGAAGCCACCAGCGTAACACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATCTTCGCACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCCGCCTCTGAACTGGTCAACAAAGCGCCGGCATTGACTGAAGCAGt                                                 >  1:1290431/1‑289 (MQ=255)
                                                                                                                                                                                                                                            cGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTa                                                                                                                                                                                                                 <  2:864725/126‑1 (MQ=255)
                                                                                                                                                                                                                                            cGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTa                                                                                                                                                                                                                 >  1:864725/1‑126 (MQ=255)
                                                                                                                                                                                                                                            cGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTAcc                                                                                              <  1:1333541/241‑1 (MQ=255)
                                                                                                                                                                                                                                            cGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTg                                                                                 >  1:921898/1‑254 (MQ=255)
                                                                                                                                                                                                                                                     tGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCt                                     <  1:1326463/289‑1 (MQ=255)
                                                                                                                                                                                                                                                      gCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCa                                                               >  2:507809/1‑262 (MQ=255)
                                                                                                                                                                                                                                                      gCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCa                                                               <  1:507809/262‑1 (MQ=255)
                                                                                                                                                                                                                                                           tGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCAcc                                >  1:566209/1‑288 (MQ=255)
                                                                                                                                                                                                                                                           tGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATgtgt                                                                                                                                                                                                      <  1:1349576/122‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tctcATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGa                   >  2:182452/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                             ggATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGt                                                 >  1:1016983/1‑253 (MQ=255)
                                                                                                                                                                                                                                                                                 gAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTcgc         <  1:988558/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 gAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTcgc         >  2:1358102/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                   aaGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTcgcg        >  1:681135/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                       cgcgcAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCa     <  1:807517/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        gcgtAGACCATTGAAGCCGCCAGGGTAAAACAACGGGCTGACGTTGGCGTGCGGGTATGGGATACAGTGCGTATTAGCGGTACGGTAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCGGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTg  <  1:1239353/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                             
TATTTCACTCGGTATTATCGACCTGGTCTATCCCGCACACTGGCAGCCATACATTGCTCAGGATCTCGGTCAACAAACCGATATCGATATCTATCTCGACCGCCATGTAGTGCGTCAGGGACGATATCTTTCGCTGCATGATGAAGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTG  >  NC_000913/3527306‑3527874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: