Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	898,433	(CTG)2→1	2.8%	coding (445‑447/489 nt)	ybjO →	DUF2593 family inner membrane protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	898431 =	105 (0.890)	3 (0.030)	3/494	NT	2.8%	coding (443/489 nt)	ybjO	DUF2593 family inner membrane protein
	?	NC_000913	= 898427	105 (0.910)					coding (439/489 nt)	ybjO	DUF2593 family inner membrane protein

TTTCAACGCTACCACTCACCACCATTTTGGCTTTATTACGAAACCCTTGTTCCGGGCCTGACACCGGCGCGCACCATTCCTCAACCGGAAAGTCGGCGAGCAGATTTTTAAGATCGGCGGTTTTAGCGGAGAGTTGCTCTGGAATCGGCTGCATTATCCACTGACAGGAACGACAGCGACCCGCGTCGTAAAGTGCGCACTGCATACATTGACCTTCACATCATCAGGGGCGACGATTATACACATTATTGCAACTGGAAGAACCGCCGACTGGTCGAGGGAACGAA‑‑‑CAGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/898722‑898431 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcaTCAGGATGAGCATATCCGGCAGCTTCTGCAGCATCAGGCTATGGAAGATTTCACGTTTTGATTCACCGGGAATGCTGAACAGCTCCGGATAACCGTACCCCAGCGAAGCCGCCCACAAGTAACTTGCGGCGGTGATTTGTGTCAGCAGATATAGCCAGCGCGCCCAGCGACGCCCTTTCACCAGTGAAAACGCACACCAGATCTCAATGAAAACCAGCAC  <  NC_000913/898427‑898208                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     TTTCAACGCTACCACTCACCACCATTTTGGCTTTATTACGAAACCCTTGTTCCGGGCCTGACACCGGCGCGCACCATTCCTCAACCGGAAAGTCGGCGAGCAGATTTTTAAGATCGGCGGTTTTAGCGGAGAGTTGCTCTGGAATCGGCTGCATTATCCACTGACAGGAACGACAGCGACCCGCGTCGTAAAGTGCGCACTGCATACATTGACCTTCACATCATCAGGGGCGACGATTATACACATTATTGCAACTGGAAGAACCGCCGACTGGTCGAGGGAACGAA‑‑‑CA                                                                                                                                                                                                                                 <  2:697175/289‑1  TTCAACGCTACCACTCACCACCATTTTGGCTTTATTACGAAACCCTTGTTCCGGGCCTGACACCGGCGCGCACCATTCCTCAACCGGAAAGTCGGCGAGCAGATTTTTAAGATCGGCGGTTTTAGCGGAGAGTTGCTCTGGAATCGGCTGCATTATCCACTGACAGGAACGACAGCGACCCGCGTCGTAAAGTGCGCACTGCATACATTGACCTTCACATCATCAGGGGCGACGATTATACACATTATTGCAACTGGAAGAACCGCCGACTGGTCGAGGGAACGAA‑‑‑CAG                                                                                                                                                                                                                                <  1:139253/289‑1                                 TTATTACGAAACCCTTGTTCCGGGCCTGACACCGGCGCGCACCATTCCTCAACCGGAAAGTCGGCGAGCAGATTTTTAAGATCGGCGGTTTTAGCGGAGAGTTGCTCTGGAATCGGCTGCATTATCCACTGACAGGAACGACAGCGACCCGCGTCGTAAAGTGCGCACTGCATACATTGACCTTCACATCATCAGGGGCGACGATTATACACATTATTGCAACTGGAAGAACCGCCGACTGGTCGAGGGAACGAA‑‑‑CAG                                                                                                                                                                                                                                >  1:118288/1‑258                                 TTATTACGAAACCCTTGTTCCGGGCCTGACACCGGCGCGCACCATTCCTCAACCGGAAAGTCGGCGAGCAGATTTTTAAGATCGGCGGTTTTAGCGGAGAGTTGCTCTGGAATCGGCTGCATTATCCACTGACAGGAACGACAGCGACCCGCGTCGTAAAGTGCGCACTGCATACATTGACCTTCACATCATCAGGGGCGACGATTATACACATTATTGCAACTGGAAGAACCGCCGACTGGTCGAGGGAACGAA‑‑‑CAG                                                                                                                                                                                                                                <  2:118288/258‑1                                                                  GGCGCGCACCATTCCTCAACCGGAAAGTCGGCGAGCAGATTTTTAAGATCGGCGGTTTTAGCGGAGAGTTGCTCTGGAATCGGCTGCATTATCCACTGACAGGAACGACAGCGACCCGCGTCGTAAAGTGCGCACTGCATACATTGACCTTCACATCATCAGGGGCGACGATTATACACATTATTGCAACTGGAAGAACCGCCGACTGGTCGAGGGAACGAA‑‑‑CAGCATCAGGATGAGCATATCCGGCAGCTTCTGCAGCATCAGGCTATGGAAGATTTCACGTTTTGA                                                                                                                                                                 >  1:422640/1‑288                                                                  GGCGCGCACCATTCCTCAACCGGAAAGTCGGCGAGCAGATTTTTAAGATCGGCGGTTTTAGCGGAGAGTTGCTCTGGAATCGGCTGCATTATCCACTGACAGGAACGACAGCGACCCGCGTCGTAAAGTGCGCACTGCATACATTGACCTTCACATCATCAGGGGCGACGATTATACACATTATTGCAACTGGAAGAACCGCCGACTGGTCGAGGGAACGAA‑‑‑CAGCATCAGGATGAGCATATCCGGCAGCTTCTGCAGCATCAGGCTATGGAAGATTTCACGTTTTGA                                                                                                                                                                 <  2:422640/288‑1                                                                                                                                                                                                                                    GGGCGACGATTATACACATTATTGCAACTGGAAGCACCGCCGACTGGTCGAGGGAACGAACAGCAGCATCAGCATGAGCATATCCGGCAGCTTCTGCAGCATCAGGCTATGTAAGATTTCACGTTTTGATTCACCGGGAATGCTGCACAGCTCCCGATAACCGTACCCCTGCGACGCCGCCCACACGTAACTTGCGGCGGTGATTTGTGTCAGCTGATATAACCAGCGCGCCCAGCGACGCCTTTTCACCGGTGAAACCTCCCACCcactccccatgaaaccaagccc  >  2:745229/1‑266                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     TTTCAACGCTACCACTCACCACCATTTTGGCTTTATTACGAAACCCTTGTTCCGGGCCTGACACCGGCGCGCACCATTCCTCAACCGGAAAGTCGGCGAGCAGATTTTTAAGATCGGCGGTTTTAGCGGAGAGTTGCTCTGGAATCGGCTGCATTATCCACTGACAGGAACGACAGCGACCCGCGTCGTAAAGTGCGCACTGCATACATTGACCTTCACATCATCAGGGGCGACGATTATACACATTATTGCAACTGGAAGAACCGCCGACTGGTCGAGGGAACGAA‑‑‑CAGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/898722‑898431 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagcaTCAGGATGAGCATATCCGGCAGCTTCTGCAGCATCAGGCTATGGAAGATTTCACGTTTTGATTCACCGGGAATGCTGAACAGCTCCGGATAACCGTACCCCAGCGAAGCCGCCCACAAGTAACTTGCGGCGGTGATTTGTGTCAGCAGATATAGCCAGCGCGCCCAGCGACGCCCTTTCACCAGTGAAAACGCACACCAGATCTCAATGAAAACCAGCAC  <  NC_000913/898427‑898208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.