Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,528,106 T→G 24.0% L547R (CTG→CGG)  yrfF → putative RcsCDB‑response attenuator, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,528,1060TG24.0% 92.0 / 35.5 50L547R (CTG→CGG) yrfFputative RcsCDB‑response attenuator, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (20/18);  new base G (5/7);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 7.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

GCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCC  >  NC_000913/3527833‑3528393
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                               
gCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCGGGATAACCTGGTGGc                                                                                                                                                                                                                                                                                  <  2:237979/289‑1 (MQ=255)
             tAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACcgcg                                                                                                                                                                                                                                                                     <  2:360022/289‑1 (MQ=255)
                     aaGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTAGTACGCCCGGTGGGTGCGGAAGCGCTGGATAAcc                                                                                                                                                                                                                                                                                         >  1:384510/1‑261 (MQ=255)
                                  cgTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACcgcg                                                                                                                                                                                                                                                                     <  1:35907/268‑1 (MQ=255)
                                  cgTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACcgcg                                                                                                                                                                                                                                                                     >  2:35907/1‑268 (MQ=255)
                                        tGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCGGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTAACACGCATTAAACGGcccgc                                                                                                                                                                                                                                          >  2:96548/1‑288 (MQ=255)
                                         gCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGcgcg                                                                                                                                                                                                                                           <  2:375544/287‑1 (MQ=255)
                                               ttACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGcac                                                                                                                                                                                                                                   <  2:266004/289‑1 (MQ=255)
                                               ttACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCGGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGcac                                                                                                                                                                                                                                   <  2:289999/289‑1 (MQ=255)
                                                 acgttacacAATTCAAAAATCCGGCATGGTATTGCTTGATGTTTTTGGCTTCATTATACTGAATACATCGGATTTATGTTCTGCCAAAGATTACTGTGTGCGACTAAAAAATGCGCTAGTCAATCTCGGCAACAGTAAAGACTTGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCGGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACaa                                                                                                                                                                                                                                 <  1:269043/281‑1 (MQ=255)
                                                    ttCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACcgcg                                                                                                                                                                                                                                                                     >  1:403619/1‑250 (MQ=255)
                                                    ttCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACcgcg                                                                                                                                                                                                                                                                     <  2:403619/250‑1 (MQ=255)
                                                         aaaTTCAAAAGTCCGGCTGGGTTTTGTTTAAGGATTTTGTCGAAATTGGACGAAAGACAGCGGATTTATGTTCTGCCAAAGGTGACTGTGTCGGACTGAAAAAGGCGCTGGTCAATCTCGGCAACAGTAAAGACGGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTaaa                                                                                                                                                                                                                         <  2:5449/288‑1 (MQ=255)
                                                            ttCAAAATTCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAAAAg                                                                                                                                                                                                                      >  1:5449/1‑289 (MQ=255)
                                                            ttCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGCGCGGCACAATCACTAAACAg                                                                                                                                                                                                                      >  1:281673/1‑289 (MQ=255)
                                                            ttCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACa                                                                                                                                                                                                                       >  1:117738/1‑288 (MQ=255)
                                                                 aaaTCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCtggtg                                                                                                                                                                                                                                                                                    <  2:177556/222‑1 (MQ=255)
                                                                 aaaTCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCtggtg                                                                                                                                                                                                                                                                                    >  1:177556/1‑222 (MQ=255)
                                                                                        tgatTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTTTCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGTCCCCCGGCCGATTCCtcgttg                                                                                                                                                                                            >  2:20990/1‑282 (MQ=255)
                                                                                                              aaGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCGGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTttg                                                                                                                                                                    >  2:92142/1‑289 (MQ=255)
                                                                                                                   aGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCACCAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATACCCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAACCGGCGCGAGCGGCACAAACACTAACCAGTCCGGCCCCCGGCGGATTCCTGATTGCCCGCGACGACGGCAGCTATTttgttg                                                                                                                                                                 >  2:328571/1‑287 (MQ=255)
                                                                                                                   aGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGcgcgg                                                                                                                                                                                                                                          >  1:224663/1‑213 (MQ=255)
                                                                                                                   aGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGcgcgg                                                                                                                                                                                                                                          <  2:224663/214‑2 (MQ=255)
                                                                                                                   aGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGa                                                                                                                                                                >  2:326663/1‑288 (MQ=255)
                                                                                                                   aGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCGGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGAt                                                                                                                                                               >  1:382473/1‑289 (MQ=255)
                                                                                                                      ggATTTATGTTCTGCCAAAGATGACTGGGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGAt                                                                                                                                                               <  2:295552/286‑1 (MQ=255)
                                                                                                                        aTTTATGTTCTGCCAAAGATGACTGTTTGGGACTGAAAAATGCGCTGTGCAAGCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCGGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGGGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGAt                                                                                                                                                               <  2:306341/284‑1 (MQ=255)
                                                                                                                              gtccgtccAAAAGTGACGGGGTGAGACTGAAAAATGCGCTGGTCAATCCCGGCAACGGTAAAGACTTGGACGAGCTGGTAAACCGCGCGAACGCCGGTAAGCTCGATGGCGGGAATGTGTTATTCTGCCCGGTGTGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCCCCGCGCCGTTTATTACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCTGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGcc                                                                                                                                                    <  2:60371/283‑1 (MQ=255)
                                                                                                                                  tGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGGGCGGAATCGCGGGATAACCTGGCGGCAACCGACACCGCGCCGTTTATCAAGCATGAAACGGCTCGAGCGGCACAATCACTAAACCGTCCGGCCCCCGGCGGGTTCCTGATTGTCAGCGACGAAGGCAGGGATTTTGTTTGTCCGCCCTGGCCttg                                                                                                                                                 >  1:306341/1‑287 (MQ=255)
                                                                                                                                    ccAAAGATGACTGTGTGCGACTGAAAAATGCGCTGATCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCCCGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGAGTCCTGATTGTCAGCGACGAAGGCAGCGCTTTTGTTGATCAGCCCTGGCCTTCgg                                                                                                                                               >  1:58929/1‑288 (MQ=255)
                                                                                                                                      aaaGATGACTGTGAGCGACTGAAAAATGCGCGGGTCAATCTCGGCAAAAGTAAAGACTGGGACGCACTGGTAAAACGCGCCAACGCCGGGAAGCTCAATGGCTTGAATGTGTTATTACGCCCGGTGAGTGCTGAATCGCGGGATAACCTGGTGGAAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCAt                                                                                                                                            <  1:96548/289‑1 (MQ=255)
                                                                                                                                            gACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACttt                                                                                                                                      >  1:203866/1‑289 (MQ=255)
                                                                                                                                               tgtgtgCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCGGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTAc                                                                                                                                    <  1:92142/288‑1 (MQ=255)
                                                                                                                                                                   gcTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGGTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTGTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGAACAGCCATGGCCTTCGGCATCCATTTCAGACTACCCGCCGCAAGAACaa                                                                                                               >  1:336129/1‑288 (MQ=255)
                                                                                                                                                                                               tGGGACGCGCTGGTAAAACGGGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTACTTACGCCGGGTGAGTGCGAAAGCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGACACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATGGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACaaa                                                                                   <  1:29803/289‑1 (MQ=255)
                                                                                                                                                                                                gggACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGTCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCCGATTCCTGATTGTCAGCGACGAAGGCAGCAATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTCCCCGCCGCAAGAACAGTGGAACGCTTTCACGAAACTCGCACAAAt                                                                                  >  1:97816/1‑289 (MQ=255)
                                                                                                                                                                                                 ggACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAAt                                                                                  >  2:105777/1‑288 (MQ=255)
                                                                                                                                                                                                  gACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACAGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTACAGAAACTGGCACAAAt                                                                                  >  1:202035/1‑287 (MQ=255)
                                                                                                                                                                                                      cgcTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCACGGTGAGTGCGGAATCGCTGGATGACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAACAACAGTGGAACGCTTTCAAGAAACTGGCACAAATGCTGAt                                                                            >  2:249021/1‑289 (MQ=255)
                                                                                                                                                                                                             aaaaCGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAATGCTGATGCATACg                                                                     <  2:281673/289‑1 (MQ=255)
                                                                                                                                                                                                                     ccAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGcac                                                                                      >  2:347209/1‑264 (MQ=255)
                                                                                                                                                                                                                     ccAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGcac                                                                                      <  1:347209/264‑1 (MQ=255)
                                                                                                                                                                                                                     ccAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCGGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAATCATGATGCATCCGCCGTTTa                                                              >  2:386139/1‑288 (MQ=255)
                                                                                                                                                                                                                         cGCCGGGAAGCTCGATGGGGAGAATGGGTTATTACGCCCGGTGGGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGcc                                                         <  1:328571/289‑1 (MQ=255)
                                                                                                                                                                                                                          gCCGGGAGGCTGGATTGCGTGAATGGGTTATTACGCCCGGTGAGGGCGGAAGCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTGGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCg                                                        <  2:58929/289‑1 (MQ=255)
                                                                                                                                                                                                                           ccGGGAAGGTGGATGTCGGGATTGTGTTATTACCGCCGGTGAGTGCGGAATCGCTGGATAACCGGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACAGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCg                                                        <  2:202035/288‑1 (MQ=255)
                                                                                                                                                                                                                                              gAATGTGTTCTTCGGCCCGTTGAGTACGGAAGCGCTGCATAACCTGGGGGCACCCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGGTCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATc                                    <  2:97816/289‑1 (MQ=255)
                                                                                                                                                                                                                                                             cccGGTGAGTGCGGAATCTCTGGATAACCTGGTGGCAACCTCCACCGAGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTGGATCAGCCCTAGCCTTCGGCATCACTTTACGCATACCCGCCGCAAGCACAGTGGAACGCTTTCCAGAAAATGGCACAAATGCTGATGCCTACGCCGTTTAACGCCGAGGGTATCTCCACAAAAATCTTCACTTACCCCAAt                     >  1:370047/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                         ggAATCGCGGGATAACCTGGTGGCAACCTCACCCGGGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACgcagca          <  2:382473/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                            aTCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCatat       <  1:326663/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 tGGATAACCTGGTGGCAACCTCCACCGCGCCTTTTATACAGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGcc  <  2:117738/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                               
GCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACTGAAGACAGCGGATTTATGTTCTGCCAAAGATGACTGTGTGCGACTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTATCACGCATGAAACGGCGCGAGCGGCACAATCACTAAACAGTCCGGCCCCCGGCGGATTCCTGATTGTCAGCGACGAAGGCAGCGATTTTGTTGATCAGCCCTGGCCTTCGGCATCACTTTACGACTACCCGCCGCAAGAACAGTGGAACGCTTTCCAGAAACTGGCACAAATGCTGATGCATACGCCGTTTAACGCCGAGGGTATCGTCACAAAAATCTTCACTGACGCCAATGGTACGCAGCATATTGGCC  >  NC_000913/3527833‑3528393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: