Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	284,128	A→G	14.8%	T310A (ACC→GCC)	yagF →	CP4‑6 prophage, dehydratase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	284,128	0	A	G	14.8%	66.5 / 3.2	27	T310A (ACC→GCC)	yagF	CP4‑6 prophage, dehydratase family protein
Reads supporting (aligned to +/- strand):  ref base A (13/10);  new base G (2/2);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

CCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCG  >  NC_000913/283874‑284412                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                             cctgcgcctCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGccc                                                                                                                                                                                                                                                             >  2:357341/1‑288 (MQ=255)                         ggcAGTCCCGCGGCACGGCGGGCACCGCGCAGGTGGTGCCGGGGGGGCTGGGGCTGGGGCGGCCGCACTCCGCGCTGGCGCCGTCCAGGCAGGCGGTGGGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGGGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCgg                                                                                                                                                                                                                                     <  2:148778/286‑1 (MQ=255)                                             ggCCCCTCGCAGGGGGTCGGGGGGGCGCTGGGTCTGGCGCTGGCGCAGTCCGCGCGGGCGCCGTCCGGGCAGGCGGTGGGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACgcgc                                                                                                                                                                                                                 <  1:60544/288‑1 (MQ=255)                                                 ccTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCaa                                                                                                                                                                                                            <  1:357341/289‑1 (MQ=255)                                                            gtCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTg                                                                                                                                                                                                  >  1:321304/1‑288 (MQ=255)                                                                                                  gcTGGGGCCGGCCGGGCCGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGTGCGGTCAGCGAGCTGGATAGCCGCGGCAGCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACAGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTa                                                                                                                                                            <  1:85508/288‑1 (MQ=255)                                                                                                            tCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGAACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCACGGACTATCCCCCGAcct                                                                                                                                                 >  2:82087/1‑288 (MQ=255)                                                                                                                ggCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTAGCCCCCGACCGTgc                                                                                                                                              >  1:122200/1‑288 (MQ=255)                                                                                                                                                                      tGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCCCCTTCCTCGCGGGCGGCGTGCCGGCGGGGGTGCTCCCCCCGCGCGCCCCCg                                                                                        >  2:11254/1‑288 (MQ=255)                                                                                                                                                                       ggATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCgg                                                                                       >  1:188177/1‑288 (MQ=255)                                                                                                                                                                                cgGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCGCCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCctg                                                                                   <  1:27476/283‑1 (MQ=255)                                                                                                                                                                                cgGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCGCCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCctg                                                                                   >  2:27476/1‑283 (MQ=255)                                                                                                                                                                                  gCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCGCCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGt                                                                                                               <  1:310854/253‑1 (MQ=255)                                                                                                                                                                                  gCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCGCCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGt                                                                                                               >  2:310854/1‑253 (MQ=255)                                                                                                                                                                                    aTCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATGTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTg                                                                          <  2:321304/288‑1 (MQ=255)                                                                                                                                                                                      cacacagcgGGGTATCCTTTCCGATAAAGCCATGGAAAGCGGGATGGTGGTCCACGCGGCGTTCGGCGGCTCCACCAATTTCCTGCTGCACAGTCCGGCCAGCGCCCACGCGGCGGGCTGCACGATCGCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGGGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGa                                                                        <  2:403774/282‑1 (MQ=255)                                                                                                                                                                                       accacGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGCCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCCCGGCCTGCTGCATCTGGAc                                                                       >  1:59680/1‑288 (MQ=255)                                                                                                                                                                                           cgcgGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGCGCGTGCTGCCCAACGGCCCGGACTCTCACCCGCCCGTGCGCGCCTTCCCCCCGTGCGGCGTGCCGGAGGCGATGCCCCCCCTGCGCCCCCCCGGCCTGCTGCATCCGGACGCCag                                                                  >  2:362133/1‑288 (MQ=255)                                                                                                                                                                                                    tCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCAtggccgtgac                                                         <  1:11254/289‑1 (MQ=255)                                                                                                                                                                                                        ctcCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGc                                                     <  1:139662/289‑1 (MQ=255)                                                                                                                                                                                                          ccGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAAGCGTAAAGTGCCGCGCCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGCGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCCTCTGGAAGCCATGACCCTGACCGGccc                                                   >  1:186702/1‑288 (MQ=255)                                                                                                                                                                                                          ccGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGCGGTGATGCCCCACCTGCGCGCCCTCGGCCTGCTGCATCTGGACGCCATGACCGGGCCCGGcc                                                    >  2:139662/1‑288 (MQ=255)                                                                                                                                                                                                          ccGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGGGATGCTCCCCCCGCGCGACCTCGGCCTGCTGAATCTCGACGCCATGACCGTGACCGGccg                                                   >  1:313268/1‑288 (MQ=255)                                                                                                                                                                                                                      tCGAAACCGCGCTGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCCGACGTTGAGCACTGGACGCGCCTCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCga                                       <  1:246311/289‑1 (MQ=255)                                                                                                                                                                                                                           gcccgcgtGGGGATCCAGGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCAGCGCCCACGCGGCGGGCTGCACGGTCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAAcc                                  <  2:205029/282‑1 (MQ=255)                                                                                                                                                                                                                                               ggcgTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCGGCTGCCTCTGGACGCCATGACCGGGACCGGCCAGCCGGTGGGCGAGCACCTTGACTGGTGGCAGGCGTcc              >  1:182097/1‑289 (MQ=255)                                                                                                                                                                                                                                                           cTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCCCGAACCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCAGACCGTGCGCGCCTTCCTCTCGGGCGGCGTGCCGGAGGGGATGCTCCACCGGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGCAGGTCGGCGAGAACCCTGAATGATGGCAGGCGTCCGAGCGCCGGgcg  >  1:352154/1‑289 (MQ=255)                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                             CCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCGAAAACGCGATGGTGATCCACGCGGCGTTCGGCGGCTCCACCAATTTACTGCTGCACATTCCGGCCATCGCCCACGCGGCGGGCTGCACGATCCCGGACGTTGAGCACTGGACGCGCATCAACCGTAAAGTGCCGCGTCTGGTGAGCGTGCTGCCCAACGGCCCGGACTATCACCCGACCGTGCGCGCCTTCCTCGCGGGCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCG  >  NC_000913/283874‑284412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑