mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.32.0 revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | pgi-12-1_S23_L001_R1_001.good.fq | 414,092 | 111,364,188 | 100.0% | 268.9 bases | 289 bases | 99.8% |
| errors | pgi-12-1_S23_L001_R2_001.good.fq | 414,092 | 111,392,311 | 100.0% | 269.0 bases | 289 bases | 98.9% |
| total | 828,184 | 222,756,499 | 100.0% | 269.0 bases | 289 bases | 99.3% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,653 | 46.9 | 3.6 | 100.0% | Escherichia coli str. K-12 sbstr. MG1655, complete genome. Live strain from Systems Biology Research Group |
| total | 4,641,653 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 2191 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 157 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.020 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.92961 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | OFF |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.025 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.2.8 |
| R | 3.3.1 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 23:06:46 18 Mar 2018 | 23:06:59 18 Mar 2018 | 13 seconds |
| Read alignment to reference genome | 23:07:00 18 Mar 2018 | 23:08:08 18 Mar 2018 | 1 minute 8 seconds |
| Preprocessing alignments for candidate junction identification | 23:08:08 18 Mar 2018 | 23:08:23 18 Mar 2018 | 15 seconds |
| Preliminary analysis of coverage distribution | 23:08:23 18 Mar 2018 | 23:09:01 18 Mar 2018 | 38 seconds |
| Identifying junction candidates | 23:09:01 18 Mar 2018 | 23:09:04 18 Mar 2018 | 3 seconds |
| Re-alignment to junction candidates | 23:09:04 18 Mar 2018 | 23:09:15 18 Mar 2018 | 11 seconds |
| Resolving best read alignments | 23:09:15 18 Mar 2018 | 23:09:42 18 Mar 2018 | 27 seconds |
| Creating BAM files | 23:09:42 18 Mar 2018 | 23:10:12 18 Mar 2018 | 30 seconds |
| Tabulating error counts | 23:10:12 18 Mar 2018 | 23:10:31 18 Mar 2018 | 19 seconds |
| Re-calibrating base error rates | 23:10:31 18 Mar 2018 | 23:10:31 18 Mar 2018 | 0 seconds |
| Examining read alignment evidence | 23:10:31 18 Mar 2018 | 23:23:34 18 Mar 2018 | 13 minutes 3 seconds |
| Polymorphism statistics | 23:23:34 18 Mar 2018 | 23:23:34 18 Mar 2018 | 0 seconds |
| Output | 23:23:34 18 Mar 2018 | 23:23:44 18 Mar 2018 | 10 seconds |
| Total | 16 minutes 57 seconds | ||
