Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,304,521	G→C	5.4%	intergenic (+130/+585)	eco → / ← mqo	ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,304,521	0	G	C	5.4%	282.5 / 7.2	95	intergenic (+130/+585)	eco/mqo	ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base G (47/40);  new base C (2/3);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 6.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

AAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTT  >  NC_000913/2304237‑2304807                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                aagaGAAGAAATTTGTCACCGCGTATCTGGTCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCa                                                                                                                                                                                                                                                                                               <  1:309092/287‑1 (MQ=255)     gaagaaATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCg                                                                                                                                                                                                                                                                                          <  1:952324/288‑1 (MQ=255)     gaagaaATTTGTCACCGCGTATCGGGGCGATGCTGGAATGCTGCGTGACAACAGCAAGCTGCCGAGCGGGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACGGCCGTGAAGTGCGGCACCCCGTAGGGCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCAcccc                                                                                                                                                                                                                                                                                           <  2:89700/287‑1 (MQ=255)      aagaaATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTa                                                                                                                                                                                                                                                                                        >  1:686541/1‑289 (MQ=255)          aaTTTTTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATGGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTTCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAgg                                                                                                                                                                                                                                                                                      <  1:138675/287‑1 (MQ=255)                  aCCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAg                                                                                                                                                                                                                                                                             >  1:264118/1‑288 (MQ=255)                    cgcgTATCTGGGCGATGCTGGAATGCTGCGTTACAACATCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAATGCGGTCACGCCGCATCCGACATCCTACGCCCGAGCCGGT‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACACCGCTGTCAAGTGCGTCACCCCGTACGTCAGACAAGTCg                                                                                                                                                                                                                                                                          >  1:657249/1‑289 (MQ=255)                    cgcgTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCg                                                                                                                                                                                                                                                                          >  1:670474/1‑289 (MQ=255)                          tCTGGGCGATGCTGGAATGCTGCTTTACAACAGCAAGCTGCCGATCGTGGGGTATACGCCAGACAATGGAGGTGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAAGTGAC‑AACGCGGTAGTGCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAcg                                                                                                                                                                                                                                                                    <  1:633889/289‑1 (MQ=255)                           cTGGGCAGTGATGGAATGCTGCGTTACACCAGCAAGCTGCCGATCGTGGTGTATACGCCAGACATTGTAGATGTGAAGTACCGGGTCTGGAAGGG‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACGGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCAGCCGACATCCAACGCCCGGGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAcgc                                                                                                                                                                                                                                                                   <  1:393057/289‑1 (MQ=25)                             ggggGATGCTAGAATGCTGCATTACAACAGCAAGCTGCCGAGCGTGGTGTAAACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAATTGCGGCACCCCGTAGGGCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAcgccg                                                                                                                                                                                                                                                                 <  1:687541/289‑1 (MQ=255)                              ggCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGt                                                                                                                                                                                                                                                                                     <  2:259127/268‑1 (MQ=255)                              ggCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGt                                                                                                                                                                                                                                                                                     >  1:259127/1‑268 (MQ=255)                                   tGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                                                                                                                                                                                                                                                                              >  1:632817/1‑270 (MQ=255)                                   tGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                                                                                                                                                                                                                                                                              <  2:632817/270‑1 (MQ=255)                                             ctgcGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAAc                                                                                                                                                                                                                                                  >  1:818262/1‑288 (MQ=255)                                             ctgcGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCCTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACACCCAAc                                                                                                                                                                                                                                                  >  1:459376/1‑288 (MQ=255)                                              tgcGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACg                                                                                                                                                                                                                                                 >  2:1171478/1‑288 (MQ=255)                                                     caacaGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGcc                                                                                                                                                                                                                                         >  1:728521/1‑289 (MQ=255)                                                        caGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGt                                                                                                                                                                                                                                      >  1:440290/1‑289 (MQ=255)                                                          gCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCCACCCGGtt                                                                                                                                                                                                                                     >  1:738678/1‑288 (MQ=255)                                                             aGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGCGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTTAAGTGCGGCACCCCGTAGGTCGGACAAGGCGGTCACGCCCCATCCGACACCCAACGCCCGACCCGGTTGccc                                                                                                                                                                                                                                 >  2:939292/1‑288 (MQ=255)                                                                tGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGAt                                                                                                                                                                                                                              >  1:1116816/1‑289 (MQ=255)                                                                   cGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCg                                                                                                                                                                                                                           >  1:340928/1‑289 (MQ=255)                                                                          ggtgTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTgg                                                                                                                                                                                                                   <  1:934462/289‑1 (MQ=255)                                                                          ggtgGATACGCCAGAAAATGTAGATGTTAAGTACCGCGGCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACTCGGTAGTTCTCTAAATGGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAAGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTgg                                                                                                                                                                                                                   <  1:943724/289‑1 (MQ=255)                                                                           gggtagccgcgagacataagagagctgAAGTAGCGCGTGTGGCAGGC‑AGAAGAGAAAATGGAC‑ACCGGGGTAGTTCGCGAAACGTCCGTGAAGTGGGGCACACCGTAGGTCAGATAAGGCGCGAAGGCCGTATCCGACATCGAACGCCCGAGCCGGT‑TGCCTAATGCGCCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGTCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTgg                                                                                                                                                                                                                   <  2:575017/263‑1 (MQ=17)                                                                                taCGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGcgcg                                                                                                                                                                                                               >  1:1225336/1‑287 (MQ=255)                                                                                    ccAGACAATGTGGATGCGCAGTAACTGGTCAGGAAGGG‑GGAAG‑GGAAACTGACAAACGCGTGGGTTGGCTAAACTACAGTGAAGTGCGGCACCCCGGAGGTCAGCAAAGGCGGCCACGCCGCAGCCGACACCCACCGCCCGGGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTGATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  2:599398/289‑1 (MQ=14)                                                                                     cAGACAATGTGGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGGGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATc                                                                                                                                                                                                        <  1:806749/289‑1 (MQ=255)                                                                                     cAGACAATGGAAAGATGGCGTACCGGGCCTGGAGGGC‑GGAAGAGACACTTGAC‑GTCGTGGGGGTTCGCTGAACTGCCGTGAAGGGCGGCACCCCGTGGGTCAGACAAGGCGGTCACGCAGCGTCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTGATCAGGCCTAAACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  2:340928/288‑1 (MQ=17)                                                                                     aagaaagtgtgGATGTAAAGTCGCGAGTGTGGAGGGG‑GGAAGAGCAAATTGGC‑ACCTGGGGAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTGGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGGGCCGGT‑GGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  2:962401/281‑1 (MQ=17)                                                                                      aggggacagcgtgtggatgtgccGCGTCT‑GAAGGCGGGAAGTGGAATTTGTC‑ACCTCGGAGGTGCGCTAAACGGCCGTGACGCTCGCAACCCCGAGGGTCAGACAAGGCGGTCAAGCCGCATCGGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  2:1196685/266‑1 (MQ=14)                                                                                       gACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCAGACATCCAACGCCCGAGCCGGTTGCCTGATGCA‑ACGCTGGCGCGTCTTAtagg                                                                                                                                                                                                      >  1:577134/1‑286 (MQ=255)                                                                                        aCAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCTACGCTGGCGCGTCCTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACCCCGCCTCCGACATCCAACGCCCGCGCCGGTTGCCTGATGGC‑ACACTGGCCCGTCCTAACAg                                                                                                                                                                                                      >  2:913359/1‑288 (MQ=21)                                                                                        aCAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAg                                                                                                                                                                                                      >  1:123064/1‑288 (MQ=255)                                                                                               gaatgtgagggagcagtgcTGGAAGGC‑GGAAGGTAAAATGGAG‑ACGGGGGGAGTGAGCTAAATTGCCGTGAGGTGGGGCACCCCGTAGGTGAGACAAGGGGGGCACGCCGCAGCCGACAGAAAACCCCCGGGCGGGT‑GGCCTGAGGCGAAGCTGGCGCGTCTGATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                                                                                                               <  2:335278/270‑1 (MQ=14)                                                                                               aGATGTGAGTTACGCGGTCTGGAAGGC‑TGAATGTAAAATGGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGTTCACGCCGCATCCGACATCCATCGCCCGGGCCGTT‑TGCCTGGTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                                                                                                               <  1:214521/288‑1 (MQ=17)                                                                                                  tgtgAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACACGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGCTGCG‑CCGCTGGCGCCTCTTATCCGGCCTACAcc                                                                                                                                                                                             >  1:508939/1‑287 (MQ=255)                                                                                                   gtgAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTCCAcc                                                                                                                                                                                             >  1:76749/1‑286 (MQ=255)                                                                                                   gtgAAGTACCGCGGCTGGAAGGA‑GGAAGAGAAAATTGAC‑AACGCGGAAGTTCGCTAAACTGCCGTGAAGGGCTGCACCCCGTAGGTCAAACAAGGCGGTCACGCCGCATCCGACAGCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGATGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGGCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCt                                                                                                                                                                                          <  1:320320/289‑1 (MQ=255)                                                                                                      aaGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGTGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGCCATCCAACGCCCGCGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTCTCAGGCCTACACCGCtgtg                                                                                                                                                                                       >  1:335278/1‑289 (MQ=255)                                                                                                      aaGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCtgtg                                                                                                                                                                                       >  1:352060/1‑289 (MQ=255)                                                                                                      aaGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCAGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCtgt                                                                                                                                                                                        >  1:706386/1‑288 (MQ=255)                                                                                                        gTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGaa                                                                                                                                                                                     >  2:858165/1‑289 (MQ=255)                                                                                                          aCCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACACGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                                                                                                               >  1:1196685/1‑277 (MQ=255)                                                                                                           ccGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGACACCCCGTAGGTCAGACAAGGCGGTCACGCCGCTACCGACATCCCACACCCGACCCGCTTGCCTGATGCGCACG‑TCGCGCGTCTCATCAGTCCCACCCCGTCGTGAAgc                                                                                                                                                                                   >  2:819926/1‑287 (MQ=18)                                                                                                              cgTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCt                                                                                                                                                                                >  1:925627/1‑288 (MQ=255)                                                                                                              cgTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCt                                                                                                                                                                                >  1:1252365/1‑288 (MQ=255)                                                                                                              cgTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCt                                                                                                                                                                                >  1:446792/1‑288 (MQ=255)                                                                                                                  gtgagaggggaaaGGGAAACTGGGC‑ACCGCGGGGGTTCGCTAAAGCGCCGTGAAGAGCGGCACCCGGTAGGTCAGACAAGGCGGTCACGCCGCGTCCGACATCCAACGCCCGAGCCGGT‑GGCCTGATGCGACGCTGGCGCGTCGTATCAGGCCTAAACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCa                                                                                                                                                                             <  2:264118/277‑1 (MQ=14)                                                                                                                         c‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                                                                                                                                                                    >  1:705848/1‑289 (MQ=255)                                                                                                                                   aaaTTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                                                                                                                                                            <  1:858165/288‑1 (MQ=255)                                                                                                                                       tGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCg                                                                                                                                                                        <  1:904907/272‑1 (MQ=255)                                                                                                                                       tGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCg                                                                                                                                                                        >  2:904907/1‑272 (MQ=255)                                                                                                                                                      tgtgctaactcggcgggAAGTGCGGCACCCCGAAGGTAAGAAAAGGCGGTCAGACCGCGTCCGAGATCCAACGCCTGAGCCGGT‑GGCCTGAGGCGACGCTGGCGCGTCTTAACAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                                                                                                                          <  2:123064/272‑1 (MQ=14)                                                                                                                                                           tAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAt                                                                                                                                                              <  1:331109/263‑1 (MQ=255)                                                                                                                                                           tAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAt                                                                                                                                                              >  2:331109/1‑263 (MQ=255)                                                                                                                                                            aaccttccGTTAATTGCGGCACCCCCTGGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGTGCCGGT‑TGCCTGATGCGACGCGGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCt                                                                                                                                   <  1:1142326/283‑1 (MQ=255)                                                                                                                                                             aaCTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGACGGAGAAGGCGGTGACGCCGGATCCGACATCTa                                                                                                                                  >  1:247206/1‑289 (MQ=255)                                                                                                                                                                        aGTGCGGCACCCCGTAGGTCAGAAAAGGCGGTCACGCCGCCTCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                        <  1:913359/288‑1 (MQ=255)                                                                                                                                                                           caggCACCCGTCAGGTTGGACAAGGCGGCAAGGCCACATCCGTCATACAACCCCCGAGCCGGT‑GGCCGGATGGGACGCTGGCGCGTCTTAACAGGCCTAAACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCGGAGAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCgg                                                                                                                     <  2:738678/286‑1 (MQ=14)                                                                                                                                                                            cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                   <  1:1171478/289‑1 (MQ=255)                                                                                                                                                                            cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGGTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                   >  1:563177/1‑289 (MQ=255)                                                                                                                                                                               ccccccGTAGGTCAGGTAAGGCGGTCACGCCGCGTGCGGCATCGAAGGGCCGAGAGGGA‑TGCCGGGGGGGAAGCTGGCAGGTGGGAGCAGGGCTACACCGCTGTGAAGGGCGGCACCCCGTGGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                 <  2:670474/286‑1 (MQ=14)                                                                                                                                                                               cACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                                                  >  1:460140/1‑287 (MQ=255)                                                                                                                                                                                 ccccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  1:397357/198‑1 (MQ=21)                                                                                                                                                                                    cGTAGGTCAGACAAAGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCCGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACCCCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCCTCCGCCACACAACGCCCCAGCCGGTTGCCTGTTg                                                                                                           >  1:355619/1‑289 (MQ=14)                                                                                                                                                                                         gTCAGCCAAGGCGGTGACGCCGCCTCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                                                                                                                          <  1:359186/253‑1 (MQ=255)                                                                                                                                                                                           cAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                                                                                                               >  2:397357/1‑198 (MQ=21)                                                                                                                                                                                            aGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTg                                                                                                   >  1:10743/1‑289 (MQ=255)                                                                                                                                                                                              aCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAAGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATACGGCGGTTACGCCGCATCCGACATCGAACTCCCTAGCCGGTTGCCTGATGCGACGCTGgc                                                                                                 >  1:132602/1‑289 (MQ=255)                                                                                                                                                                                                       cccggccgcACCCGGCACCCAAC‑CCCGTGGCGGTGCGCGTGAGGGGACTCGGGCACGTCTGATCTGGCCTAGCCGGCGGTGAAGTGCGCGACCCCGTAGGGCCGACAAGGCGGTCACGCCGCATCCGACACCCAACGCCCGGGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTGATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                        <  2:577134/285‑1 (MQ=11)                                                                                                                                                                                                                   cgccATCCACCGCCCGAGCCGGT‑TGCCGGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                              <  1:308041/284‑1 (MQ=255)                                                                                                                                                                                                                                    ggcggggtGCCTGGTGGGACGCTGCGGCGTCTTCTGAGCCCTACACCGCCGTGAAGGGCGGCACCCCGTGGGTCAGACAAGGGGGTCACGCCGCATCCGACATCCAACGCCCGGGCCGGTGGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTcc                                                             <  2:925627/281‑1 (MQ=11)                                                                                                                                                                                                                                    gCCGGT‑AGCGTGATGAGACGCTGGGGCGTGTGATCATGCCTAAACCGCTGGGAAGTGCGGCACCCCGGAGGTCATAAAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCa                                                            <  2:705848/288‑1 (MQ=11)                                                                                                                                                                                                                                         t‑tGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                                                                                                                          >  2:134500/1‑205 (MQ=255)                                                                                                                                                                                                                                         t‑tGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                                                                                                                          <  1:134500/205‑1 (MQ=255)                                                                                                                                                                                                                                             ccgatggggacagggggacgggttAGCAGGCCTAAACCGCTGGGAAGGGCGGCGCCCCGGAGGTCAGACGAGGCGGGCACGACGCAGCCGAGAGCCAACGCCCGAGCCGGTTGCCGGATGCG‑AAGCTGGCGCGGCTTTTCAGGCCTACCCCGCTGTGAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTGGCCGGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                                                    <  2:1252365/266‑1 (MQ=11)                                                                                                                                                                                                                                                     gACGCGGGCGCGTCTTATCAGGACTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATaa                                           <  1:819926/289‑1 (MQ=38)                                                                                                                                                                                                                                                            gcgcGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTCGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCCGCTGCGACGCTGGCGCGTACTATCACGCCTCCCCCGCTGTGAACTCCCACACCCCAGAGGTTggctcagcgcgg                                     >  2:842405/1‑278 (MQ=11)                                                                                                                                                                                                                                                               gtacTTTTCGGGCCTAGACCGCGGTGAAGGCGGGCACCCCGTAGGTCGGGCAAGGCGGGCACGCCGCAGCCGACGTCCAACGCCCGGGCCGGTGGCAGGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTAcg                                 <  2:728521/286‑1 (MQ=11)                                                                                                                                                                                                                                                                      tCAGGCCCACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGACGGATAAGGCGGTTACGCCGCATCCGACACCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGAGAAGTGCTCCAACCCGTAGGTTAGATGAGGCGGTTACGCCGCAt                           >  1:32839/1‑288 (MQ=255)                                                                                                                                                                                                                                                                               cacTGCTGGGAAGTGCGGCACCCCGTAGGTCGGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCGGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCa                 <  1:784898/289‑1 (MQ=255)                                                                                                                                                                                                                                                                               cacCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACCTCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCa                 >  1:110869/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                  cGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACg              <  1:781851/289‑1 (MQ=40)                                                                                                                                                                                                                                                                                    cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGTCTCATGCGACGCTGGCCCGTCTTATCAGGCCTACACCGCTGAGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGGTACCCCGGATTCGACATCGAACGt             >  1:72807/1‑288 (MQ=255)                                                                                                                                                                                                                                                                                    cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGt             >  1:279219/1‑288 (MQ=40)                                                                                                                                                                                                                                                                                    cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACgc             >  1:853962/1‑287 (MQ=25)                                                                                                                                                                                                                                                                                        gacgggCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGCCATCCACCGCCCGCGCCGGTTGCCTGATGCG‑ACGCTGGCCGGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa         <  2:1043044/283‑1 (MQ=255)                                                                                                                                                                                                                                                                                         aaGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAg        >  1:959788/1‑288 (MQ=255)                                                                                                                                                                                                                                                                                         aaGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAg        >  1:350481/1‑288 (MQ=21)                                                                                                                                                                                                                                                                                            tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGCCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCAGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGCAGTTCTCCACCCCGCAGGTTGGATAAGTCGGTTACGCCGCATCCGACCTCCAACGTCCGAGCCgg    >  1:283291/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                              cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                >  1:1251310/1‑211 (MQ=39)                                                                                                                                                                                                                                                                                              cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                <  2:1251310/211‑1 (MQ=39)                                                                                                                                                                                                                                                                                               ggCACCCGGGAGTGCGGACAGGGCGGTAAGGCCGCAGCGGAGGGCAAGCGCCCTAGCCGGTTGCCGGAGGCG‑AAGCTGGCGGGGCATAACAGGCCTAAACCGCTGTGAAGTGCCGCACCCCGGAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTATCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGGCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGtt  <  2:508939/288‑1 (MQ=11)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                AAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTT  >  NC_000913/2304237‑2304807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑