Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,526,445 G→A 4.0% intergenic (‑298/‑22) nudE ← / → yrfF adenosine nucleotide hydrolase, Ap3A/Ap2A/ADP‑ribose/NADH hydrolase/putative RcsCDB‑response attenuator, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,526,4450GA 4.0% 572.0 / 7.6 168intergenic (‑298/‑22)nudE/yrfFadenosine nucleotide hydrolase, Ap3A/Ap2A/ADP‑ribose/NADH hydrolase/putative RcsCDB‑response attenuator, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (78/83);  new base A (4/3);  total (82/86)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 188 total aligned reads displayed.

ATGCTGTGAAATCAGCGGTTATTGTGCGATATCGGACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTGCTGGCCTGCTCACTACTTGCGGGATGGCTGATAAAAGTGCGATCCAGACGGCGTCAGCTGCCCTGGACCAACGCCTTCGCGGATGCGCAAACGCGTAAACTCACACCTGAAGAACGTAGCGCCGTTGAAAATTATCTTGAGAGCCTGACGCAGGTATTACAGGTGCCTGGCCCAACGGGAGCCAGCGCGGCACCGATCTCTCTGGCGCTGAATGCCGAAAGCA  >  NC_000913/3526158‑3526722
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                     
aTGCTGTGAAATCAGCGGTTATTGTGCGATATCGGACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGa                                                                                                                                                                                                                                                                                      <  1:169543/289‑1 (MQ=255)
    tgtgAAATCAGCGGTTATTGTGCGATATCGGACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACtaa                                                                                                                                                                                                                                                                                  >  2:1007677/1‑289 (MQ=255)
      tgAAATCAGCGGTTATTGTGCGATATCGGACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGg                                                                                                                                                                                                                                                                                <  2:118994‑M2/289‑2 (MQ=255)
      tgAAATCAGCGGTTATTGTGCGATATCGGACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGg                                                                                                                                                                                                                                                                                <  2:833778‑M2/289‑2 (MQ=255)
                ggTTATTGTGCGATATCGGACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaa                                                                                                                                                                                                                                                                      >  1:833778‑M2/1‑278 (MQ=255)
                ggTTATTGTGCGATATCGGACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaa                                                                                                                                                                                                                                                                      >  1:280084‑M2/1‑278 (MQ=255)
                         gCGATATCGGACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagaccc                                                                                                                                                                                                                                                              >  2:1088666‑M2/1‑269 (MQ=255)
                                cGGACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatga                                                                                                                                                                                                                                                      <  2:882495‑M2/289‑28 (MQ=255)
                                       gCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGATGATATTGAGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTCCGCCTGACCACGCCTGCCAGACTAAGgaaggttcgaacaagtccctgatatgagatcatg                                                                                                                                                                                                                                               <  2:237200‑M2/289‑35 (MQ=255)
                                                 aTGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaa                                                                                                                                                                                                                                                                      <  1:735151‑M2/256‑12 (MQ=255)
                                                   ttgaATTCCATGTTATTTCCATTTATCTAGCGTAAAAAAGAAGCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATACCTCCAGGGATTTACTGATATTCCGCCTTCAGAGGGATTGATATGTTCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAGACACCACGCCTGCCGATAGCCAGCCGCAGATTCATGTATTGTGTCCGGTGCGACTGACCGCGCATGACAGACTGAGgaaggtgcgaactagtccgtgatatgagatcatgtttgacagctgg                                                                                                                                                                                                                                   >  2:847491‑M2/2‑243 (MQ=255)
                                                   gtgAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCGGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCtt                                                                                                                                                                                                                                   >  2:426817/1‑289 (MQ=255)
                                                     taatTGCATGTTATTTACAATTATGTAACTTAATAAATAATCGTCCCCAAATCAAATTAAAAGTCAATAGGGTGAAATAACTCCAGGAATTTTCTGAGATTCCGCCTTCAGGGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATTCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggag                                                                                                                                                                                                                                 >  2:474067‑M2/2‑241 (MQ=255)
                                                          gCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTGCTGGc                                                                                                                                                                                                                            <  1:538528/289‑1 (MQ=255)
                                                              gTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaac                                                                                                                                                                                                                        >  1:882495‑M2/1‑232 (MQ=255)
                                                                          tATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcat                                                                                                                                                                                                            <  1:178909‑M2/289‑70 (MQ=255)
                                                                            tGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatga                                                                                                                                                                                                          <  2:22510‑M2/289‑72 (MQ=255)
                                                                               aaCTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtc                                                                                                                                                                                                       >  1:395290‑M2/1‑215 (MQ=255)
                                                                                aCTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCGTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTGCTGGCCTGCTCACTACTTGCGGGATgg                                                                                                                                                                                                      >  2:901011/1‑289 (MQ=255)
                                                                                 cTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtca                                                                                                                                                                                                      <  1:886375‑M2/288‑76 (MQ=255)
                                                                                 cTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTGCTGGCCTGCTCACTACTTGCGGGa                                                                                                                                                                                                         <  1:1051345/285‑1 (MQ=255)
                                                                                 cTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTGCTGGCCTGCTCACTACTTGCGGGa                                                                                                                                                                                                         >  2:1051345/1‑285 (MQ=255)
                                                                                               gTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgc                                                                                                                                                                                       >  1:1007677‑M2/1‑199 (MQ=255)
                                                                                                tCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTACGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcctgagtcatcaacttaccttcgcc                                                                                                                                                                                      <  2:1082409‑M2/289‑92 (MQ=255)
                                                                                                  cTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccga                                                                                                                                                                                    >  1:1010690‑M2/1‑196 (MQ=255)
                                                                                                      aatcaaatTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagt                                                                                                                                                                                >  2:1079861‑M2/1‑192 (MQ=255)
                                                                                                              tAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCGTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTGCTGGCCTGCTCACTACTTGCGGGATGGCTGATAAAAGTGCGATCCAGACGGCGTCAg                                                                                                                                                                        <  1:901011/289‑1 (MQ=255)
                                                                                                                 aaGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtc                                                                                                                                                                                                       >  1:952695‑M2/1‑181 (MQ=255)
                                                                                                                      aaTAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagc                                                                                                                                                                <  2:945409‑M2/289‑114 (MQ=255)
                                                                                                                      aaTAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtcc                                                                                                                                                                                                                                                               >  1:1019532‑M2/1‑176 (MQ=255)
                                                                                                                           gTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccg                                                                                                                                                            <  2:364292‑M2/288‑118 (MQ=255)
                                                                                                                           gTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccg                                                                                                                                                            <  1:421319‑M2/288‑118 (MQ=255)
                                                                                                                              gAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaaca                                                                                                                                                                                                                                                                    >  1:984824‑M2/1‑168 (MQ=255)
                                                                                                                                     cTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacaggg                                                                                                                                                                                                                    >  1:745394‑M2/1‑161 (MQ=255)
                                                                                                                                          ggAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGATGCTTTGCTGGCCTGCTCACTACTTGCGGGATGGATGATAAAAGTGCGATCCAGACGGCGTAAGCTGCCCTGGCCCACCGCCTTccgggagg                                                                                                                                            >  2:103580/1‑282 (MQ=255)
                                                                                                                                                tGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTACGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaacagagattt                                                                                                                                        <  1:776095‑M2/287‑138 (MQ=255)
                                                                                                                                                           cGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgacccgcat                                                                                                                           >  1:22510‑M2/1‑139 (MQ=255)
                                                                                                                                                               ttCGGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATCGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTGCTGGCCTGCTCACTACTTGCGGGATGGCTGATAAAAGTGCGATCCAGACGGCGTCAGCTGCCCTGGACCAACGCCTTCGCGGATGCGCAAACGCGTAAACTCacac                                                                                                                       <  2:460346/289‑1 (MQ=255)
                                                                                                                                                                          tGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgcc                                                                                                            <  1:1132624‑M2/289‑166 (MQ=255)
                                                                                                                                                                          tGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgcc                                                                                                            <  1:684945‑M2/289‑166 (MQ=255)
                                                                                                                                                                          tGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagat                                                                                                                  <  2:27506‑M2/283‑160 (MQ=255)
                                                                                                                                                                          tGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggag                                                                                                                                                                                                                                 >  1:797918‑M2/1‑124 (MQ=255)
                                                                                                                                                                          tGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggag                                                                                                                                                                                                                                 <  2:797918‑M2/172‑49 (MQ=255)
                                                                                                                                                                          tGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtcc                                                                                                                                                                                                                                                               <  2:1202836‑M2/142‑19 (MQ=255)
                                                                                                                                                                          tGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtcc                                                                                                                                                                                                                                                               <  1:1096985‑M2/142‑19 (MQ=255)
                                                                                                                                                                          tGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtcc                                                                                                                                                                                                                                                               >  2:1096985‑M2/1‑124 (MQ=255)
                                                                                                                                                                                  tCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTGCTGGCCTGCTCACTACTTGCGGGATGGCTGATAAAAGTGCGATCCAGACGGCGTCAGCTGCCCTGGACCAACGCCTTCGCGGATGCGCAAACGCGTAAACTCACACCTGAAGAACGTAGCGCCGt                                                                                                    >  1:491150/1‑289 (MQ=255)
                                                                                                                                                                                         ttACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgaga                                                                                                                                                                                                                                                    <  2:495687‑M2/138‑30 (MQ=255)
                                                                                                                                                                                                    tgCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACCAAGTAACATGGGGAAAGCATGAGCCCCATTGTGATTTTTTTAGCTGCTTTGCTGGCCTGCTCACTACTTTAGGGATGGCTGATAAAAGTGCGATCCAGACGGCGTCAGCTGACCTGGAACAACGCCTTCGCGGATGCGCAAACGAGTAAACTCACCACTGAAGACCGCAGCGCCGTGGAAAATTAACTTGtgag                                                                                  >  2:522852/1‑289 (MQ=255)
                                                                                                                                                                                                       ttAATGATGCTAAAGTTAAACTCCACGCGTGCCGATAGCCATCAGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtcgctaatatgagaccatgtttgtcatctggagccatagaacagggttcatcatgcgtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagc                                                                               >  1:269023‑M2/1‑95 (MQ=255)
                                                                                                                                                                                                                cTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgag                                                                                >  2:955442‑M2/1‑86 (MQ=255)
                                                                                                                                                                                                                cTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagagtctgccatggcaaaacatgggggaagtcatcgagccgttttac                                                                       <  1:642668‑M2/288‑203 (MQ=255)
                                                                                                                                                                                                                   aaGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatatggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtagaagtcatcgagccgttttacccca                                                                   <  2:1109200‑M2/289‑207 (MQ=255)
                                                                                                                                                                                                                   aaGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTGCTGGCCTGCTCACTACTTGCGGGATGGCTGATAAAAGTGCGATCCAGa                                                                                                                                                                                 >  1:699909/1‑179 (MQ=255)
                                                                                                                                                                                                                      ttAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTTACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaagg                                                                >  2:886375‑M2/1‑80 (MQ=255)
                                                                                                                                                                                                                         aaCTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccctaaggctg                                                             <  2:177245‑M2/289‑213 (MQ=255)
                                                                                                                                                                                                                             ccACCCTTGCCCATAGCCAACCACAGAATCATGTTTTGTGTTCGGTGCGACTGACCACGCCTGACAGACTAAGtaaggttcgaacaattcccttatatgagttcatgtttgtcatctgtggccagagaacagggttcatcatgagtcagcaacttaccttcgccgacagtgagttcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagaagattctgccatgtcaaagaatggtggaagtcagcgagccgttttaccccaaggctggtaa                                                         >  2:776095‑M2/1‑73 (MQ=255)
                                                                                                                                                                                                                               aCGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtaatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatg                                                       <  2:437174‑M2/289‑219 (MQ=255)
                                                                                                                                                                                                                                cGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatgg                                                      <  1:1210089‑M2/289‑220 (MQ=255)
                                                                                                                                                                                                                                cGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaagg                                                                >  1:834979‑M2/1‑70 (MQ=255)
                                                                                                                                                                                                                                    tGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccgg                                                  >  1:1080330‑M2/1‑66 (MQ=255)
                                                                                                                                                                                                                                     gCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaccagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttttcccgcatggagcagattctgccatggaaaaacatggtggaagtcaccgagctgttttacccaaaggctggtaatggccggc                                                 <  2:545561‑M2/289‑225 (MQ=255)
                                                                                                                                                                                                                                          tAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgacct                                            <  2:510250‑M2/289‑230 (MQ=255)
                                                                                                                                                                                                                                           aGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggt                                                           <  2:1254470‑M2/273‑215 (MQ=255)
                                                                                                                                                                                                                                           aGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggt                                                           >  1:1254470‑M2/1‑59 (MQ=255)
                                                                                                                                                                                                                                                            tGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccacggctggtaatggccggcgaccttatccgctggaacccat                           <  1:631506‑M2/288‑247 (MQ=255)
                                                                                                                                                                                                                                                                   tgtCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgttatccccagtttttagtgagatctctcccactgacgtatcatttggtccgcccgaaacaggttggccagcgtgaataacatcgccagttggttatcgtttttcagc                                  >  1:343078‑M1/1‑35 (MQ=255)
                                                                                                                                                                                                                                                                   tgtCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaataagcggggaaattcttctcggctgactcagtcatttcatttcttcatgtttgagccgattttttctcccgtaaatgccttgaatcagcctatttagaccgtttcttcgccatttaaggcgttatccccagtttttagtgagatctctcccactgacgtatcatttggtccgcccgaaacaggttggccagcgtgaataacatcgccagttggttatcgtttttcagc                                  <  2:343078‑M1/274‑240 (MQ=255)
                                                                                                                                                                                                                                                                   tgtCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaacacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgca                   <  2:653263‑M2/289‑255 (MQ=255)
                                                                                                                                                                                                                                                                   tgtCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggcctgcgaccttatccgctggaaaccatgctacgca                   <  1:94387‑M2/289‑255 (MQ=255)
                                                                                                                                                                                                                                                                   tgtCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgca                   <  1:929250‑M2/289‑255 (MQ=255)
                                                                                                                                                                                                                                                                   tgtCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgag                                                                                <  1:318575‑M2/228‑194 (MQ=255)
                                                                                                                                                                                                                                                                   tgtCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgag                                                                                >  2:318575‑M2/1‑35 (MQ=255)
                                                                                                                                                                                                                                                                   tgtCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcacgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgca                   <  2:601761‑M2/289‑255 (MQ=255)
                                                                                                                                                                                                                                                                   tgtCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccataaaacagggttcatcatgattcatcaacttaccttcgccgacagtgacttcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccccatggagcagattctgccttggcaaaacatggtggaatccatcaagccgttttcccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgca                   <  2:927320‑M2/289‑255 (MQ=255)
                                                                                                                                                                                                                                                                   tgtCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTGCTGGCCTGCTCACTACTTGCGGGATGGCTGATAAAAGTGCGATCCAGACGGCGTCAGCTGCCCTGGACCAACGCCTTCGCGGATGCGCAAACGCGTAAACTCCCACCTCAAGAACGTAGCGCCGTTGAAAATTATCTTGAAAGCCTGACGCAGGTATTAAAGGTGCCTGGCCCAACGGGACGTAGCGCGGCACCGATCTCTCTgg                    >  2:252133/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                   tgtCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTGCTGGCCTGCTCACTACTTGCGGGATGGCTGATAAAAGTGCGATCCAGACGGCGTCAGCTGCCCTGGACCAACGCCTTCGCGGATGCGCAAACGCGTAAACTCACACCTGAAGAACGTAGCGCCGTTGAAAATTATCTTGAGCGCCTGACGCAGGTATTACAGGTGCCTGGcc                                                     >  2:424322/1‑255 (MQ=255)
                                                                                                                                                                                                                                                                    gtCCGGTGCGACTGACCACGCCTGATAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcat                  >  2:1017070‑M2/1‑34 (MQ=255)
                                                                                                                                                                                                                                                                     tCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcat                  <  1:459014‑M2/288‑256 (MQ=255)
                                                                                                                                                                                                                                                                     tCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggag                                                                                                                       <  2:295777‑M2/187‑155 (MQ=255)
                                                                                                                                                                                                                                                                     tCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggag                                                                                                                       >  1:295777‑M2/1‑33 (MQ=255)
                                                                                                                                                                                                                                                                     tCCGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagagcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggatggtaatggacggcgaccttatccgctggaaaccatgttacgcat                  <  2:743600‑M2/288‑256 (MQ=255)
                                                                                                                                                                                                                                                                      ccGGTGCGACTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcatt                 >  1:57216‑M2/1‑32 (MQ=255)
                                                                                                                                                                                                                                                                      ccGGGGCGACTGCCCACGCCTGACAGCTTAAGgaaggtgcgaccaattccctgatatgagatcatttttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggaaaaacatggtggaattcatcgagccgttttacccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcattc                 >  1:237200‑M2/1‑32 (MQ=255)
                                                                                                                                                                                                                                                                               cacttaccgcacaacaaactaaggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgcaatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcattcactgcatgc       >  1:240399‑M2/14‑23 (MQ=255)
                                                                                                                                                                                                                                                                               cacttaccgcacaacaaactaaggaaggtgcgaacaagtccctgatatgagataatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcattcactgcatgc       >  2:573432‑M2/14‑23 (MQ=255)
                                                                                                                                                                                                                                                                               cacgtaccgcacaacaaactaaggaaggtgcgaacaagtgcctgaaatgagagcagtttttacatctgaagccatagaacagggttcatcatgagtcatcaacttaccttcgacgacagttaattcagccgtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcccattctgccatggcaaaacatggttgaagtcatcgcgccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcattcactgcatgc       >  2:1065520‑M2/14‑23 (MQ=255)
                                                                                                                                                                                                                                                                               cTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcattcactgcatgc       >  1:364292‑M2/1‑23 (MQ=255)
                                                                                                                                                                                                                                                                               cTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcattcactgcatg        >  1:510250‑M2/1‑23 (MQ=255)
                                                                                                                                                                                                                                                                               cTGACCACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcagctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcattcactgcatgc       >  2:631506‑M2/1‑23 (MQ=255)
                                                                                                                                                                                                                                                                                   taccgcacaacaaactaaggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcattcactgcatgcagca   >  1:1221972‑M2/10‑19 (MQ=255)
                                                                                                                                                                                                                                                                                   taccgcacaacaaactaaggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcattcactgcatgcagca   >  1:843840‑M2/10‑19 (MQ=255)
                                                                                                                                                                                                                                                                                   ccACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcattcactgcatgcagca   >  2:684945‑M2/1‑19 (MQ=255)
                                                                                                                                                                                                                                                                                   ccACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcattcactgcatgcagca   >  1:426090‑M2/1‑19 (MQ=255)
                                                                                                                                                                                                                                                                                    cACGCCTGACAGACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggcagtcatcgagccgttttaccccaaggctggtactggccggcgaccttatccgctggaaaccatgctacgcattcactgcatgcagcat  <  1:499866‑M2/289‑272 (MQ=255)
                                                                                                                                                                                                                                                                                    accgcacaacaaactaaGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaaccatgctacgcattcact             >  1:651234‑M2/9‑18 (MQ=255)
                                                                                                                                                                                                                                                                                          caacaAACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgctggaaacc                             <  2:710285‑M2/254‑245 (MQ=255)
                                                                                                                                                                                                                                                                                          caacaAACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttt                                                                          >  1:124556‑M2/3‑12 (MQ=255)
                                                                                                                                                                                                                                                                                          caacaAACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttt                                                                          <  2:124556‑M2/209‑200 (MQ=255)
                                                                                                                                                                                                                                                                                            aCAAACTAAGgaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttt                                                                          <  1:11971‑M2/209‑200 (MQ=255)
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                     
ATGCTGTGAAATCAGCGGTTATTGTGCGATATCGGACACGCTTTCGGCAATGTGAATTGCATGTTATTTACATTTATGTAACTTAATAAATAATCGTCCTCAAATCAAATTAAAAGTCAATAGGTTGAAATAACTCCAGGAATTTGCTGATATTCCGCCTTCAGAGGGTTTGCTATGATCAGCGGTTACTGTGATGTGCTTAATGATGCTCAAGTTAAACTCCACGCTTGCCGATAGCCAACCGCAGAATCATGTATTGTGTCCGGTGCGACTGACCACGCCTGACAGACTAAGTAAGATGGGGAAAGCATGAGCACCATTGTGATTTTTTTAGCTGCTTTGCTGGCCTGCTCACTACTTGCGGGATGGCTGATAAAAGTGCGATCCAGACGGCGTCAGCTGCCCTGGACCAACGCCTTCGCGGATGCGCAAACGCGTAAACTCACACCTGAAGAACGTAGCGCCGTTGAAAATTATCTTGAGAGCCTGACGCAGGTATTACAGGTGCCTGGCCCAACGGGAGCCAGCGCGGCACCGATCTCTCTGGCGCTGAATGCCGAAAGCA  >  NC_000913/3526158‑3526722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: