Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,599,805 G→A 3.2% intergenic (‑148/+123) livJ ← / ← rpoH branched‑chain amino acid ABC transporter periplasmic binding protein/RNA polymerase, sigma 32 (sigma H) factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,599,8050GA 3.2% 429.4 / 2.5 126intergenic (‑148/+123)livJ/rpoHbranched‑chain amino acid ABC transporter periplasmic binding protein/RNA polymerase, sigma 32 (sigma H) factor
Reads supporting (aligned to +/- strand):  ref base G (66/56);  new base A (2/2);  total (68/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.48e-01

Only 100 of 127 total aligned reads displayed.

GTAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGTCCTGGCTGCGTTCGTCC  >  NC_000913/3599518‑3600087
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                          
gTAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTg                                                                                                                                                                                                                                                                                            >  1:649635/1‑288 (MQ=255)
 ttaccgccggtgcACCGTACTGCGCAACCGGGCCGGACATTGCGCCCCCGACCGCGACTTTATTATCTTCTGCCAGAGCCATGTTGCTGAATGCCAGCGCAATACATCCTGCCAGTAACGCTGTACCCTTTATGTTCATCCTGCGCATCCCCATTCTTCTGGTTATTACGTGTGTTGTAATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGCTATTTGAATAGCTATTTTGAc                                                                                                                                                                                                                                                                                          <  2:1051449/277‑1 (MQ=255)
     cTCCTGGTGACCCTACTTCGCAAACGGACCGGACATTGCGCCCAAGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATAATCTGCTAAAACATACCCTATTTGTATGATATTGGAATAGCTATTTTGACAGtt                                                                                                                                                                                                                                                                                      <  2:1006802/289‑1 (MQ=255)
          ggTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGAc                                                                                                                                                                                                                                                                                          <  1:43414/280‑1 (MQ=255)
          ggTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCCTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTttatta                                                                                                                                                                                                                                                                                 >  1:470970/1‑289 (MQ=255)
               ccGTACAGCACAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATTTTGGAATAGCTATTTTGACAGTTTATTAACAAt                                                                                                                                                                                                                                                                            <  1:1108894/289‑1 (MQ=255)
                cGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATCGCTATTTTGACAGTTTATTAACaa                                                                                                                                                                                                                                                                             >  1:324749/1‑287 (MQ=255)
                             cggaccggacATTGCGCCCACTACCGCGACTTTAATATCGTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGGTATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTg                                                                                                                                                                                                                                                              <  2:704479/289‑1 (MQ=255)
                                       aTTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGcc                                                                                                                                                                                                                                                    <  1:778244/289‑1 (MQ=255)
                                                 cggccGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGaaa                                                                                                                                                                                                                                          <  2:591332/286‑1 (MQ=255)
                                                 cgcccGCGACTGTAATATCTTCTTCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTTCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCATCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGaaa                                                                                                                                                                                                                                          <  2:776773/286‑1 (MQ=255)
                                                 cGACCGCTACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGaaa                                                                                                                                                                                                                                          <  1:722959/289‑1 (MQ=255)
                                                    ccGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAAtt                                                                                                                                                                                                                                        <  1:948493/288‑1 (MQ=255)
                                                              aaTATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGAATGACTACCCGTGCTTTAGCAGCATACTATGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACag                                                                                                                                                                                                                             >  1:191635/1‑289 (MQ=255)
                                                                    ttctGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGat                                                                                                                                                                                                                                     <  1:576934/275‑1 (MQ=255)
                                                                    ttctGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGat                                                                                                                                                                                                                                     >  2:576934/1‑275 (MQ=255)
                                                                    ttctGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGccc                                                                                                                                                                                                                       >  1:1107956/1‑289 (MQ=255)
                                                                     tctGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTCTTTTGACAGTTTATTAACACTCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGACATTGATTATTACAGAGGccac                                                                                                                                                                                                                      >  2:276021/1‑287 (MQ=255)
                                                                     tctGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCa                                                                                                                                                                                                                      >  1:84587/1‑289 (MQ=255)
                                                                         ccAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCTCGAGGGGAAATTGATTATTACAGAGGCCCaaaaa                                                                                                                                                                                                                  >  1:250250/1‑289 (MQ=255)
                                                                             agCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTCTTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAaaaaacaa                                                                                                                                                                                                              >  1:884788/1‑289 (MQ=255)
                                                                                  tatTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGCCAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGGGGCCCAaaaaacaaaaac                                                                                                                                                                                                          >  1:743914/1‑288 (MQ=255)
                                                                                       cTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGACATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCgg                                                                                                                                                                                                     >  1:1048178/1‑288 (MQ=255)
                                                                                          aaTGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGa                                                                                                                                                                                                                                                                                           <  2:447232/199‑1 (MQ=255)
                                                                                                      aTACATCCTTCCAGTAACGCTTTACCCTTTATGTTCATCCTGCGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATTTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGtt                                                                                                                                                                                     <  2:1259906/289‑1 (MQ=255)
                                                                                                      aTACATCCTGCCATTATCACTTGACCTGTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTGATTACGGTTTTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCATGCTTTAGCAGCATACTCTGCTAAAACATACCCCATTTTTATGATATTGGAATAGCTAGTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGtt                                                                                                                                                                                     <  2:743914/289‑1 (MQ=255)
                                                                                                       tACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGtt                                                                                                                                                                                     <  2:1107956/288‑1 (MQ=255)
                                                                                                       tACATCCTGCCAGTAACGCTTTACCCCTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGtt                                                                                                                                                                                     <  2:470970/288‑1 (MQ=255)
                                                                                                               gCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTCATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTACAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTGACATCCTGCGTGGGGATTGGCGTTTTGCCGGATGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTa                                                                                                                                                                            >  1:93439/1‑289 (MQ=255)
                                                                                                                            tACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAAtt                                                                                                                                                                >  1:220857/1‑288 (MQ=255)
                                                                                                                                  ttATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAACTTACGCTTc                                                                                                                                                         >  1:648835/1‑289 (MQ=255)
                                                                                                                                  ttATGTTCATCCTGAGAACCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGCATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTATAGAGGCCCAAAAAACAAACACCCCGGACTCTCATCCAGGGTTCTATGCTTAATAGCGGAAATTCCGCTTc                                                                                                                                                         >  2:404070/1‑289 (MQ=255)
                                                                                                                                         cATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTAACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGgca                                                                                                                                                  >  1:147409/1‑289 (MQ=255)
                                                                                                                                                    tCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAAttt                                                                                                                                       >  2:249582/1‑289 (MQ=255)
                                                                                                                                                      cccATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAACACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCAGGACTCTCATCCAGGGTTCTCTGCTTAATAGGGGAAATTACGCTTCAATGGCAGCACGCAAttttt                                                                                                                                     >  2:169112/1‑289 (MQ=255)
                                                                                                                                                               ctGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGtt                                                                                                                                                                                     >  2:25414/1‑232 (MQ=255)
                                                                                                                                                               ctGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGtt                                                                                                                                                                                     <  1:25414/232‑1 (MQ=255)
                                                                                                                                                               ctGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGt                                                                                                                            >  2:250436/1‑289 (MQ=255)
                                                                                                                                                               ctGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGt                                                                                                                            >  2:499835/1‑289 (MQ=255)
                                                                                                                                                                     attaCGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCttt                                                                                                                       >  1:231545/1‑288 (MQ=255)
                                                                                                                                                                       taCGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCACTTTTTTCATCGCGTTCTTTTcc                                                                                                                    >  1:88067/1‑289 (MQ=255)
                                                                                                                                                                       taCGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTcc                                                                                                                    >  1:200340/1‑289 (MQ=255)
                                                                                                                                                                       taCGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTcc                                                                                                                    >  1:555578/1‑289 (MQ=255)
                                                                                                                                                                            gtgtTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCg                                                                                                                                                                                                      >  1:165557/1‑202 (MQ=255)
                                                                                                                                                                            gtgtTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCg                                                                                                                                                                                                      <  2:165557/202‑1 (MQ=255)
                                                                                                                                                                            gtgtTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCt                                                                                                                <  1:499835/288‑1 (MQ=255)
                                                                                                                                                                            gtgtTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTg                                                                                                               >  1:1110392/1‑289 (MQ=255)
                                                                                                                                                                            gtgtTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTg                                                                                                               >  2:452341/1‑289 (MQ=255)
                                                                                                                                                                            gtgtTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTg                                                                                                               >  1:853672/1‑289 (MQ=255)
                                                                                                                                                                            gtgtTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTg                                                                                                               >  1:200224/1‑289 (MQ=255)
                                                                                                                                                                             tgttgtGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTg                                                                                                               >  1:394779/1‑288 (MQ=255)
                                                                                                                                                                             ggttgtGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAACACATACCCGATTTTTATGATATTGGAATAGCTATTATAACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCAGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAACACACCGGAGTCTCATTCAGGGTTCTCTGCTTACTAGAGGCAATTACGCTTCAATGGCAGCACGCAATTTCTCCATCGCGTTCATTCCAAGCTgg                                                                                                              >  2:691608/2‑289 (MQ=255)
                                                                                                                                                                              cttttggtgttgtTTTTCAGCACTTTATTGCGTTTTATGCAGGACTTACCGTGCGTTCGCAGCATACTCTGCTAAAACATACCCGATTTTTATGGTATTGGAATAGCTATTTTGACTGTGTATTAACAATCTGCGTGGGGATTGGAGTTTTGCGGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCCTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTgg                                                                                                              <  2:1061856/281‑1 (MQ=255)
                                                                                                                                                                                 gtgATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTa                                                                                                          <  1:250436/289‑1 (MQ=255)
                                                                                                                                                                                        tgttTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTc                                                                                                   <  2:328654/289‑1 (MQ=255)
                                                                                                                                                                                            tttCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACCCGATCcgcg                                                                                               >  2:602413/1‑289 (MQ=255)
                                                                                                                                                                                            tttCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTCCAGCTGGCGTACACGCTCAGCgg                                                                                              >  1:904678/1‑289 (MQ=255)
                                                                                                                                                                                                  cACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACg                                                                                         <  2:231545/289‑1 (MQ=255)
                                                                                                                                                                                                   aCTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGaaa                                                                                           <  2:394779/286‑1 (MQ=255)
                                                                                                                                                                                                      ttATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGt                                                                                     <  1:264677/289‑1 (MQ=255)
                                                                                                                                                                                                           tCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACgg                                                                                <  1:284460/289‑1 (MQ=255)
                                                                                                                                                                                                            cGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGt                                                                               <  2:217312/289‑1 (MQ=255)
                                                                                                                                                                                                              ttttATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGCAATTCCGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCa                                                                             >  2:704881/1‑289 (MQ=255)
                                                                                                                                                                                                               tttATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGt                                                                               >  2:308267/1‑286 (MQ=255)
                                                                                                                                                                                                               tttATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGt                                                                               <  1:308267/286‑1 (MQ=255)
                                                                                                                                                                                                                 tATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTAACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGcc                                                                          <  2:147409/289‑1 (MQ=255)
                                                                                                                                                                                                                   tGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAg                                                                        >  1:609896/1‑289 (MQ=255)
                                                                                                                                                                                                                        gACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGTGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTa                                                                                                          >  1:599375/1‑250 (MQ=255)
                                                                                                                                                                                                                        gACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCACTGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGtt                                                                      >  2:1193082/1‑286 (MQ=255)
                                                                                                                                                                                                                        gACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCGATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTa                                                                                                          <  2:599375/250‑1 (MQ=255)
                                                                                                                                                                                                                             cccGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAAc                                                              >  2:117781/1‑289 (MQ=255)
                                                                                                                                                                                                                              ccGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACg                                                             <  2:1110392/289‑1 (MQ=255)
                                                                                                                                                                                                                                gTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTg                                                           <  1:1113664/289‑1 (MQ=255)
                                                                                                                                                                                                                                   cTTTAGCAGCATACTCTGCTAAAAAATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTCCGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGGTGGCGTACCCGCCAAGCGGCAACGCCGTAACGGTCATCCAGTTACtcaaaacgagca                                                        >  2:551589/1‑278 (MQ=255)
                                                                                                                                                                                                                                   cTTTAGAAACATACTCTGCTAAAACATACCCGATTTTTAGGATATTTGAATGGCTATTTGGACAGTTTATTAACAATCTGTGTGGGGATTGACGTTTTGCCGGGGGAAAATTTAATTATTACAGAGTCCCAAAAAACAAAAACCCCGGACTCTCATCCATGGTTCTCTGCTTAATAGCAGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCAACGCGTTCTTTTCCAGCTGGCGTACACGc                                                                                                     <  2:330524/244‑1 (MQ=255)
                                                                                                                                                                                                                                        gcagcaTACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACttgtt                                                   >  1:1024767/1‑289 (MQ=255)
                                                                                                                                                                                                                                            caTACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTc                                                                                                   >  2:873669/1‑237 (MQ=255)
                                                                                                                                                                                                                                             aTACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGACCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTATGCTTAATAGCGGAAATTACGCTTCAATGGTAGCACGCAATTTTTTCATCGCGTTCTTTTCTAGCTGGCGTACACGCTCAGCGGaaa                                                                                           >  1:330524/1‑244 (MQ=255)
                                                                                                                                                                                                                                             aTACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTc                                             >  1:735765/1‑289 (MQ=255)
                                                                                                                                                                                                                                               aCTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCg                                            <  1:452341/289‑1 (MQ=255)
                                                                                                                                                                                                                                                  ctGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGc                                                                                                                                                            >  2:776992/1‑174 (MQ=255)
                                                                                                                                                                                                                                                  ctGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTAGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGc                                                                                                                                                            <  1:776992/174‑1 (MQ=255)
                                                                                                                                                                                                                                                   tGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTg                                                           >  1:48814/1‑270 (MQ=255)
                                                                                                                                                                                                                                                   tGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTg                                                           <  2:48814/270‑1 (MQ=255)
                                                                                                                                                                                                                                                             tACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGCGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTATACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAgcgcgc                              >  1:598676/1‑289 (MQ=255)
                                                                                                                                                                                                                                                             tACCCGACTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAgcgcg                               >  1:23551/1‑288 (MQ=255)
                                                                                                                                                                                                                                                               cccGATTTTTAAGAGATTGGAATAGCTATGTTGACAGTTTATTAACAATCTGCGTGGGGATAGGCGTTTTGCCGGAGGGTAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATACAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACACTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCTCAc                            <  2:200340/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                    tttttATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGgatg                       <  2:220857/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                    tttttATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAAGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCACTTTTTTCATCGCGTTCTTTTCCAGCTGGCTTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACTTGGACTTGTTGTCTTCGTCCAGCCAGCCCGCACGgatg                       >  2:394910/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                     ttttATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGgatga                      >  1:732924/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                     ttttATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGgatga                      >  1:102086/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                         aTGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGTc                  <  1:1193082/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                              atTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACg                                                                                                      <  2:1160320/200‑1 (MQ=255)
                                                                                                                                                                                                                                                                              atTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACg                                                                                                      >  1:1160320/1‑200 (MQ=255)
                                                                                                                                                                                                                                                                                   aaTAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGTCCTGGCTGCg         <  2:535667/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   aaTAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACACAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGTCCTGGCTGCg         <  1:243254/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   aaTAGCTATTTTGACAGTTTATTAACAATCTGAGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGCCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTGCGTCCAGCCAGCGCGCACGGATGATGTCCTGGCTGCg         <  1:394910/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       gCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGTCCTGGCTGCGTTCg     >  1:1020485/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                         tATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGATGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGTCCTGGCTGCGTTCGTcc  <  1:946179/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         tATTTTGACAGTTTATTAACAATCGTCGTGGGGATTGGCGTTTGGCCGGAGGGGAAATTGATCATTACATAGGCACAAAAAACAAAAACCCCGGACTCGCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAAGTTTTTCCTCGCGTTCTTTTCCAGCGGGCGTACACTCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGTCCTGGCTGCGTTCGTcc  <  2:432186/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                          
GTAAACTCCTGGTCACCGTACTGCGCAACCGGACCGGACATTGCGCCCACGACCGCGACTTTAATATCTTCTGCCAGAGCCATATTGCTGAATGCCAGCGCGATACATCCTGCCAGTAACGCTTTACCCTTTATGTTCATCCTGAGAATCCCCATTCTTCTGGTTATTACGTGTGTTGTGATGTTGTTTTTCAGCACTTTATTTCGTTTTATGCATGACTACCCGTGCTTTAGCAGCATACTCTGCTAAAACATACCCGATTTTTATGATATTGGAATAGCTATTTTGACAGTTTATTAACAATCTGCGTGGGGATTGGCGTTTTGCCGGAGGGGAAATTGATTATTACAGAGGCCCAAAAAACAAAAACCCCGGACTCTCATCCAGGGTTCTCTGCTTAATAGCGGAAATTACGCTTCAATGGCAGCACGCAATTTTTTCATCGCGTTCTTTTCCAGCTGGCGTACACGCTCAGCGGAAACGCCGTAACGGTCAGCCAGTTCCTGCAACGTGGACTTGTTGTCTTCGTCCAGCCAGCGCGCACGGATGATGTCCTGGCTGCGTTCGTCC  >  NC_000913/3599518‑3600087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: