Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,442,533 A→G 3.3% N258D (AAC→GAC)  mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,442,5330AG 3.3% 529.0 / 3.9 153N258D (AAC→GAC) mnmCfused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
Reads supporting (aligned to +/- strand):  ref base A (79/69);  new base G (3/2);  total (82/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.82e-01

Only 100 of 154 total aligned reads displayed.

CTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCTTTGCGCCAGCGAAAAACCCGGATATGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGT  >  NC_000913/2442251‑2442821
                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                  
 tGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCTTTGCGCCAGCGAAAAACCCGGATATGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCAc                                                                                                                                                                                                                                                                                             >  1:771678/1‑289 (MQ=255)
 tGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCTTTGCGCCAGCGAAAAACCCGGATATGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCAc                                                                                                                                                                                                                                                                                             >  1:994748/1‑289 (MQ=255)
        ttCGCTAAATCAACAAGTAGATGCCTGGTTTCTGGACGGCCCTGCCCCAGCGAAAAACCCGGATATGTGGATGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACACTGGCGACACTTACGTCAGCCGGTTTTGCCCGCTGCGGTTGGAAGGACGCCGGCTTCACGCTGCAAACACGTAAGGGCTTTGGGCGTAAACGGGAAAGTATTTTCGGTGTTACGGGACAGAAATTAGCAGTCCCCTGATCCGTTCCTGGGTTTAAcctccagggccg                                                                                                                                                                                                                                                                                       >  2:498990/1‑278 (MQ=255)
                           gATGCCTGGTTTCTGGACGGCTTTGCGCCAGCGAAAAACCCGGATATGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCAAGGGCAGCAGCAAACGGGAAGCGGCGa                                                                                                                                                                                                                                                                   >  1:247304/1‑289 (MQ=255)
                                 tGGTTTCTGGACGGCTTTGCGCCAGCGAAAAACCCGGATATGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTCTCCGCCGCGGTTTGCAGGACGCCGGATTAACGATGCAAAAACGTAAGGGCTTTGGGCGCGAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTGAACCGCAAGGGAAGCAGAAAACCGGAAGCGGCGATTATcg                                                                                                                                                                                                                                                             >  1:926735/1‑289 (MQ=255)
                                 tGGTTTCTGGACGGCTTTGCGCCAGCGAAAAACCCGGATATGTGGACGCAAAATCTGTTTAACGCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATcgg                                                                                                                                                                                                                                                            <  1:1564469/289‑1 (MQ=255)
                                  ggTTTCTGGACGGCTTTGCGCCAGCGAAAAACCCGGATATGTGGACGAAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATcgg                                                                                                                                                                                                                                                            <  1:856877/289‑1 (MQ=255)
                                    tttCTGGACGGCTTTGCGCCAGCGAAAAACCCGGATATGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATcggcg                                                                                                                                                                                                                                                          <  1:251271/289‑1 (MQ=255)
                                             ggCTTTGCGCCAGCGAAAAACCCGGATATGTGGACGCAAACTCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGTCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGCAAGCGGCGATTATCGGCGGTGGTAtt                                                                                                                                                                                                                                                 >  1:153817/1‑288 (MQ=255)
                                              gCTTTGCGCCAGCGAAAAACCCGGATATGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTg                                                                                                                                                                                                                                                >  1:1506774/1‑288 (MQ=255)
                                                     gcCAGCGAAAAACCCGGATATGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGcgcg                                                                                                                                                                                                                                        <  1:104148/289‑1 (MQ=255)
                                                         gCGAAAAACCCGGATATGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGAGATTATCGGCGGGGGTATTGCCAGCGCgttgt                                                                                                                                                                                                                                    >  1:627951/1‑289 (MQ=255)
                                                         gCGAAAAACCCGGATATGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACCGGAAGCGGCGATTATCGGCGGTGGTATTGCCCGCGCgttgt                                                                                                                                                                                                                                    >  1:1459905/1‑289 (MQ=255)
                                                                aCCCGGATATGTGGCAGCAAAAACTGTTAAACGCCAGGGGAAGGTTGGCGCGGCCGGGCGGCACGCTGGCGACATTTACGGCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCGTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCt                                                                                                                                                                                                                             <  2:1459905/289‑1 (MQ=255)
                                                                  cagagaatGTGCACGCAACATCTGCTGAGGGCAAGTGCAAGGTGTGCGTCTCCTGGCGGCACCCTGGCAAGATTTACGTCTGCCGGTTTTGGCCGCCGCGGTTTGCAGGACGCCCGATTCACGATGCAAAAACGTAAGTGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTgg                                                                                                                                                                                                                           <  2:672453/283‑1 (MQ=255)
                                                                    ggATATGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGgcg                                                                                                                                                                                                                         <  1:408186/289‑1 (MQ=255)
                                                                     gagatGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGgcg                                                                                                                                                                                                                         <  2:1021879/285‑1 (MQ=255)
                                                                           tgGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTAc                                                                                                                                                                                                                  >  1:124550/1‑289 (MQ=255)
                                                                           tgGACGCAAAATCTGTTTAACGCCATGGCAAGGATGGCGAGTCGGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCTGGACGCCGGATTCACGATGCAACACCGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACAGCATCCATGCTCCGCGCCGTGGTTTAACGGCACGGGCAGCAGCCAACGGTCAGCGGCGACTATCGGCGGGTGTATTGCCAGAGCGGTGTTGTCGCTGGCGCTATTag                                                                                                                                                                                                                  >  2:789710/1‑288 (MQ=255)
                                                                                gCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTGTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAACAACGTAAGGGCTTCGGGCGCAAACGGGAACTGCTTTGCGGGGTGATGGAACAGACCTTACCGCTCCCCTGCCCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGACGCGGCGATTTTCGCTGGTGGTATTGCCAGCGACttgttgccccccgccctactaagccgc                                                                                                                                                                                                             >  2:21998/1‑268 (MQ=255)
                                                                                    aaTCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTAACGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGcg                                                                                                                                                                                                         >  1:1377883/1‑288 (MQ=255)
                                                                                      tCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTgg                                                                                                                                                                                                       <  1:473912/289‑1 (MQ=255)
                                                                                            tAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTa                                                                                                                                                                                                 <  1:1149206/289‑1 (MQ=255)
                                                                                            tAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCCGGCGGTATTACGGCGCCGCTGGCGGGTa                                                                                                                                                                                                 >  2:947801/1‑289 (MQ=255)
                                                                                            gaaCGCCATGGCAAGGTGGGGGCGTCCGGGCGGCACGCTGGCGACATTGACGTCTGCCGGTTTTGGCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTa                                                                                                                                                                                                 <  2:505319/288‑1 (MQ=255)
                                                                                               cGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAAc                                                                                                                                                                                               >  1:1513233/1‑288 (MQ=255)
                                                                                               cGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCCGGGCCTTTTCCGGCGCGGCTGGCGGGTAAc                                                                                                                                                                                               >  2:473912/1‑288 (MQ=255)
                                                                                                  cATGGCAAGTTGGGGGCGTCCGGTCGGCATGCTTGCGACCTTTAAGTCTGGCGGTATGGCGCGCCGGGCTATGGATGTCACCGGCTTCACGACGGAAGAGCGTGAGGGGTTTCGGAGCCAGCGTGAAATGCTTTGCGGGTTGATGGAAGGGAGATTTCGGCTCCTCTGATCCGCGCCGTGGTGTAACTGCAGGCGCAGCAGCTAGCGGGACGCGGCGTTTAGCGTGGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCggagcaggggggcggta                                                                                                                                                                                            <  2:1377883/288‑16 (MQ=255)
                                                                                                  cATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCCCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGTGCGTTGTTGTCGTTGGCGCTATTATGGCGCGGCTCGCCGGTAACGCtt                                                                                                                                                                                           >  1:1264416/1‑289 (MQ=255)
                                                                                                    tGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCCGGTAACGCTTTa                                                                                                                                                                                         >  1:1146413/1‑289 (MQ=255)
                                                                                                    tGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCCGGTAACGCTTTa                                                                                                                                                                                         >  1:256840/1‑289 (MQ=255)
                                                                                                    tGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTa                                                                                                                                                                                         >  1:1190994/1‑289 (MQ=255)
                                                                                                                cgcgTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTgg                                                                                                                                                                                                                           >  1:333177/1‑243 (MQ=255)
                                                                                                                cgcgTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTgg                                                                                                                                                                                                                           <  2:333177/243‑1 (MQ=255)
                                                                                                                 gggTCCGGCCGGCACGCTAGGGACATTTTCGGCTGCCGGTTTTGGCCGCCGCGGTGTGCAGGACGCCGGATACACGATGCAAAAGCGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGGTGTTGGCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCACGGATGa                                                                                                                                                                             <  1:21998/286‑1 (MQ=255)
                                                                                                                 gcgTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGa                                                                                                                                                                             <  2:1146413/288‑1 (MQ=255)
                                                                                                                    tCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCGCGATGCAACAACGTAAGCGCTTTGGGAGCCCACGGGAAATGCGTTGCGGGGTGGTGGAACAGAAATTACAGACCCCCTGCACCGCGCCCTGGGTTAACCGCATGGGCACAAGGAAACGGGACGCGGCGCTTCTCGGCGGTGTGTATGGCAGCGCCCTGTTGTCGCTGGCGCTATTATGCCGCCGCTGGCAGGCAATGCTGTATGGCGCGGGTCAGGc                                                                                                                                                                          >  2:721828/1‑288 (MQ=255)
                                                                                                                                       aCATTTACGTCAGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAATGTAAGGGCTTTGGGCGCAAACGGGAAATGCGTTGCGGGGGGATGGAAAAGACATTATCGCTCCCCTGCTCCGCGCAGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATAGGTGGTGGTATTGCCAGAGCGTTGTTGTCGCTGGCGCTATTACg                                                                                                                                                                                                                 >  2:304801/1‑230 (MQ=255)
                                                                                                                                          tttACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCa                                                                                                                                                   <  2:771678/289‑1 (MQ=255)
                                                                                                                                                    ccGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAggg                                                                                                                                         <  1:368757/289‑1 (MQ=255)
                                                                                                                                                      ggTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTGGGGCGCAAACGGGAAATGCTTGGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAggggg                                                                                                                                       <  1:721828/289‑1 (MQ=255)
                                                                                                                                                        ttttGTCCGCCGCGGTTTGCAGGACGCCGGATTAACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGgcg                                                                                                                                     <  1:1375534/289‑1 (MQ=255)
                                                                                                                                                                     ggTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCACTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGtta                                                                                                                         <  1:1486631/288‑1 (MQ=255)
                                                                                                                                                                      gtgtGCAGGACGCCGGATGCACGGTGCAAAAACGGAAGGGCTGTGGGCGCAAACGGGAAATGCTTTGCGGGGGGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGttat                                                                                                                        <  2:427896/285‑1 (MQ=255)
                                                                                                                                                                          gCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGc                                                                                                                   <  1:267062/289‑1 (MQ=255)
                                                                                                                                                                                cGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTGACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCCAaca                                                                                                              >  2:1233618/1‑288 (MQ=255)
                                                                                                                                                                                 gCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGATCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGAGCTGTATCAGTTATTAAAGCAACACg                                                                                                            >  2:119373/1‑289 (MQ=255)
                                                                                                                                                                                 gCCGGATCCACGATGCAAAAACGTAAGGGCTTTGGGCGCTAACGGGACATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGACGCGGCTTAACCGTCCGAGCAGCAGACAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGCTGTTCTCGCTGGCCCTATTTCGGTCCGGCTGGCAGGTTACGCCTTCTTTCGCGGTTTATGCCACCGCATCGGGTGCTTCCGGCAATCGCTAGGGGgcctcgtctccgtcttttagctaaccc                                                                                                             >  2:713781/1‑263 (MQ=255)
                                                                                                                                                                                    ggATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATTCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATg                                                                                                         <  1:1019134/289‑1 (MQ=255)
                                                                                                                                                                                           cGATGCATAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCt                                                                                                  >  1:22396/1‑289 (MQ=255)
                                                                                                                                                                                              tGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTGACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCaa                                                                                                                 <  1:1346782/271‑1 (MQ=255)
                                                                                                                                                                                                      cGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGAACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCtt                                                                                         <  1:1435278/287‑1 (MQ=255)
                                                                                                                                                                                                       gTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCttttt                                                                                      <  2:1578910/289‑1 (MQ=255)
                                                                                                                                                                                                        tAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTGACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGGTGAGGCCCCCGCCCTGGGTGCTTCCGGCCATCGCCCGGGGGCCCTGTATCCGTTATTAAGCAAACACGATGAGgctgtaccttaacaacat                                                                                     >  2:1346782/1‑273 (MQ=255)
                                                                                                                                                                                                        tAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCttttt                                                                                      >  2:77972/1‑288 (MQ=255)
                                                                                                                                                                                                        tAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCtttt                                                                                       >  2:473441/1‑287 (MQ=255)
                                                                                                                                                                                                          aGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTTGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACCGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTtc                                                                                     >  1:714459/1‑287 (MQ=255)
                                                                                                                                                                                                               tttGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTGACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAAt                                                                               <  1:1225846/288‑1 (MQ=255)
                                                                                                                                                                                                                ttGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCTGTTATTAAGCaaa                                                                                                                <  2:1515176/254‑1 (MQ=255)
                                                                                                                                                                                                                       cAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTTGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGGCAAACCTTTTTTTCTCTAATGCGTTTACt                                                                      >  1:811411/1‑289 (MQ=255)
                                                                                                                                                                                                                       cAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACt                                                                      >  1:785594/1‑289 (MQ=255)
                                                                                                                                                                                                                        aaaCGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTgcg                                                                                                                                                                                    <  1:1201896/178‑1 (MQ=255)
                                                                                                                                                                                                                        aaaCGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCGGGGAGGTAACGCTTTATTGCGCGGGTGAGGCCTCCGCACTGGGTGCTTCCGGCAATCGCGAGGGGGCGCTGTAACGGTTATTAAGCAAACACGATGAGGCGCGAAACCGCttttacacccggtgctttatc                                                                      >  2:721280/1‑271 (MQ=255)
                                                                                                                                                                                                                               aaaGGCTTTGCGGGGTGATGGAACAGACGTTACCGCTCCCCTGGTCCGCGCCGTGGTGTAACCGAACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCtcg                                                              <  2:1506774/289‑1 (MQ=255)
                                                                                                                                                                                                                                       tGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGccccc                                                                                                                                                                      <  1:157533/177‑1 (MQ=255)
                                                                                                                                                                                                                                          ggggtggagcaacatgcatggccGCTCCACTGCCTCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGGCAGGTGGTTTTTGTCGCTGGCGCTCGTACGGCGCGGCTGGCAGGTACCGCTTTATTGCGCGGATGAGGCCCCAGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTAc                                                    <  2:134844/267‑1 (MQ=255)
                                                                                                                                                                                                                                          ggggTGATGGACCAGACATTACCGCTCCCCTGCGCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACg                                                   <  1:789710/289‑1 (MQ=255)
                                                                                                                                                                                                                                           gggTGATGGAACAGACATTCCCGCTCCCCGGCGCCGCGCCGTGGTTTAACCGAACGGGCAGCAGCAAACGGGAGGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACg                                                   <  2:1314957/288‑1 (MQ=255)
                                                                                                                                                                                                                                            ggTGATTGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGtt                                                                          <  1:1301719/264‑1 (MQ=255)
                                                                                                                                                                                                                                            ggTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGGTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATTAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGGCGTATCCGCTATTAAGCAAAAACGACGCGGCGCTAAACCGCTTTTTCCCTCATGCGTTCACTTTTGACCGTGTGTTTTCCGa                                                  >  2:587982/1‑288 (MQ=255)
                                                                                                                                                                                                                                                aTGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTa                                                                                                                                                                                                 >  2:1097522/1‑141 (MQ=255)
                                                                                                                                                                                                                                                aTGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTa                                                                                                                                                                                                 <  1:1097522/141‑1 (MQ=255)
                                                                                                                                                                                                                                                  ggAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTa                                           <  1:595714/289‑1 (MQ=255)
                                                                                                                                                                                                                                                      cAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTCAACCGCTTTTTCTCTAATTCTTTTACTTTTTCTCGTCGGTTTTACGACCAATTACCCg                                       >  1:588739/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      cAGACATTACCGCTCCACTGCTCCGCGACGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGTCTGGCAGGTAAAGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCCCCAGGGGGCGGTGTATCCGTGATTTAGCATACACGATAATGCGCTAAACCGCTTTTTCTCTTATGCGTTTACTTTTgtccctgtccctcttacacatctgacc                                        >  2:1301719/1‑262 (MQ=255)
                                                                                                                                                                                                                                                        gACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCCATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCCAATGCGTTTACTTTTTCTCCTCGGTTTTACGACCAATTACCAGtt                                     >  2:790490/1‑289 (MQ=255)
                                                                                                                                                                                                                                                        gACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGtt                                     >  2:268929/1‑289 (MQ=255)
                                                                                                                                                                                                                                                          cATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTaa                                   >  1:748539/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            ttACCGCCCCCCTGCTCCGCCCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGGAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAAt                                 <  2:1228531/289‑1 (MQ=255)
                                                                                                                                                                                                                                                              aCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGcgcg                                                                                                                                                                                                                                        >  1:359863/1‑88 (MQ=255)
                                                                                                                                                                                                                                                              aCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCtt                                                                                         <  1:821068/231‑1 (MQ=255)
                                                                                                                                                                                                                                                              aCCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCtt                                                                                         >  2:821068/1‑231 (MQ=255)
                                                                                                                                                                                                                                                                 gcgccccTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGAt                           <  1:1439152/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGAt                           <  2:114892/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                  tacctgccagccGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATg                                                                                                         <  2:395311/201‑1 (MQ=255)
                                                                                                                                                                                                                                                                   tCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGAt                           >  1:332902/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                   tCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCa                         >  1:127991/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                    cctgccagccGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGtt                                     <  2:189852/269‑1 (MQ=255)
                                                                                                                                                                                                                                                                    cctgccagccGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGtt                                     >  1:189852/9‑277 (MQ=255)
                                                                                                                                                                                                                                                                      ccTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCTGCGATTATCGGCGATGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGTGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGa                      <  1:713781/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             cgcgCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTgc               >  1:513267/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                               cgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTg                                                                                                                                                                                                        <  2:829661/103‑1 (MQ=255)
                                                                                                                                                                                                                                                                               cgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCACTCGCCAGGGGGCGCTGTAACCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTTTAATGCGTTTACTTTTGCTCGTCGGGTTTACGCCCCATTACACGTTAAATTTGCATCTGACTGGTgcg              >  1:547194/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                               cgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGCTTAGGCGCTAAACCGCTTTTTCTCTAATTCGTTTACTTTTGCTCGTCCGTTTTACGACCAATTACCCGTTAAATTTGATCATGCCTGGTgcgg             >  2:1215937/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                               cgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGTGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGCTCCATTACCCGTTAAATTTGATCATGCCTgggtcg              >  2:1128498/1‑284 (MQ=255)
                                                                                                                                                                                                                                                                               cgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTgcgg             >  1:157929/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                               cgcCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAAAACGATGAGGCGCTAACCCGCCTTTTCCCTAATGCGTTTACTTTTGCCCGTCGGTTTTACGCCCATTCAACCGTTACATTTTCTCATCACTTGTgcg              >  2:1208357/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                    tGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTc         >  1:1578282/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                    tGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGAATGGTGCGGCGTc         >  1:167816/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                    tGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGCATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTctct                                                                                  <  2:254815/216‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                  
CTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCTTTGCGCCAGCGAAAAACCCGGATATGTGGACGCAAAATCTGTTTAACGCCATGGCAAGGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCGGTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTTTGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGCGGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATTGCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAGGCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGACTGGTGCGGCGTCACGCAGT  >  NC_000913/2442251‑2442821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: