Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,154 T→C 12.4% intergenic (+361/+283) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,1540TC12.4% 97.7 / 6.5 57intergenic (+361/+283)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (22/24);  new base C (4/3);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCC  >  NC_000913/4295870‑4296432
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                      
cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTAGTAGGTCGGATAAGGCGTTTACGTCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATCTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGcc                                                                                                                                                                                                                                                                                     >  1:64642/1‑288 (MQ=21)
     gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGACTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGAGTGATGTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGACTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGTATAAGTCGTTTATGCCGCATcc                                                                                                                                                                                                                                                                               >  1:1272934/1‑289 (MQ=255)
          cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTATGTCGGATAAGGCGTTGATGCCGCATCCGACa                                                                                                                                                                                                                                                                           >  1:1423105/1‑288 (MQ=21)
           gcAATATCCTGATTTAGCGTGATTTTGTATGTCGGATAAGGCATTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCTTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAAGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATc                                                                                                                                                                                                                                                                         <  1:879336/289‑1 (MQ=19)
               tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGTGACGCTTGCCGCGTCTTATCAGGCCTACGCCACACAGCGCAATAGCCTTATTCAACGTGATTTTTTAACTCGGATAATGCGATTCGTCCGCATCCGCCATCAAc                                                                                                                                                                                                                                                                      >  2:1197539/1‑288 (MQ=11)
                  ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAACCGGCGCAATAGCCTGAATTAGCTTCATTTTGTGTGTCGGAAAATGCGATTACGCCCCCTCCGACCTCAACGcc                                                                                                                                                                                                                                                                   >  2:225926/1‑288 (MQ=11)
                    tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACACCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGa                                                                                                                                                                                                                                                                >  2:1421099/1‑289 (MQ=25)
                      aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAt                                                                                                                                                                                                                                                               >  2:1311116/1‑288 (MQ=25)
                           gCGTGCTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGGCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGCTTTTGTAGGTCGGACAAGGCGTTTACGCCCCATCCGACCTCAACGCCTGATGCGACGCTTGACGCGCCCTATCAGTCCTTACCGACACAGCGCAATCGCCTGAATCAGCGTCTCTTAGTGGGTAGGATACGTCCTTTCAGTCGCATctcaccttactctcctatcctag                                                                                                                                                                                                                                                          >  2:1459065/1‑266 (MQ=11)
                            cgcgATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAATGCTTGATGCTACGCTTAACGCGTCTTTTCAGGCCTACGCCAGACATCGCATTAGCCTCATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTTACGCCGCCTCTCACATCAACGCCTGATGCGACGGCTGCTGCGTCTTATCCGGCCTACCCCACTCCGAGGCATCAGCAGCATTCTCGCCCTCTTGTACGTCTGCTTAGACGTTTATGCCACATCCGACATCCACGCCAGTTGCGatg                                                                                                                                                                                                                                                         >  2:1566554/4‑286 (MQ=2)
                                aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                                                                                                                    <  1:548214/289‑1 (MQ=37)
                                     gTAGGTTGTATAAGAGGGTCATGCCGCGCCCGAGATGAACGCCAGTTGCGACGCTGAACGGGTCTTATCAGGCCTGAGACAGAACGCGCAATAGCATGATTTAGCGTGATTTTGTAGGTCGGATAAGGGGTTTACGCCGCAGCCGACATCAACGCCTGATGCGACGCTGGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt                                                                                                                                                                                                                                               <  2:1107157/289‑1 (MQ=11)
                                           tggaagaGGCGGTTATCGCGCAGCCGAAATAAAGGCCTGAGGGTAGGATTAGCCGGTCTTATGAGGGTGACGCCAGGGTGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTTGGTTAAGGCGTTTACGCCGCGACCGGCATCAACGCCTGATGCGACGCTGGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTAt                                                                                                                                                                                                                                          <  2:505166/282‑1 (MQ=11)
                                           cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCCCAAGCAGCGCCATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGGCCTTTAAGCCCCATCCGACTTCAACCCCTGATTCTAAGCTTTAcgccgctttaa                                                                                                                                                                                                                                         >  2:656241/1‑281 (MQ=11)
                                               tAAGGCGTTTCTCCCGCTCACTTCATCAACGCCTGATGGGACGCTTAACCCGACTTATCAGGCGTCCGCCAGACAGCGAAATAGCTGGATTTGACGTCATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGGCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                     <  2:873159/289‑1 (MQ=11)
                                               tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                     <  1:579005/289‑1 (MQ=37)
                                               acaGGCGTTTATGCCGCAACGTGCATCAAAGCCTGATGCGACGCTTAACGCGTGTTTTCGGGCCTACGCCAGAGAGCGAAATAGCCTGATTTAGCGTGATTTTTTGGGTCGGGTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                     <  2:788004/287‑1 (MQ=11)
                                                gcggcatttcggtgcaccccgttgtCAACGCCTGAGTCGCAGCTTAACGCTGCTTATGCGGCTTATGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTGAGTCGGAGAAGGCATTTCCACCGCATCCGAGAGCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGGCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                     <  2:927377/264‑1 (MQ=11)
                                                aaGGGGTTTGTTCCGAATCAGGCATAAAACACTGATGCGACGCTAATGGTGTCTGATCAGGGCTACGGCAGACGGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGACAAGGTGTTTACGCCGCATCCGACGTCAACGCCTGATGCGACGATTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                     <  2:179406/288‑1 (MQ=11)
                                                       ttATGCCGGCACCGACATCAACGCCGGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTATCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGTCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCa                                                                                                                                                                                                                             <  2:1096533/289‑1 (MQ=255)
                                                         gtgtcGCAGCAGACAGCAACGCATGACGCGAAGCTTGACGGGTCTTATCAGGGTTACGCCAGGAAGCGAGATGGCCTGAGTTAGCGTGATTTGGTGGGTCGGATAAGGCGTGTACGCCGGATCCGGCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGa                                                                                                                                                                                                                           <  2:1130241/285‑1 (MQ=11)
                                                         aTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACACCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGa                                                                                                                                                                                                                           <  1:1421099/289‑1 (MQ=37)
                                                             cGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAg                                                                                                                                                                                                                        <  1:1311116/288‑1 (MQ=35)
                                                               cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAgcgc                                                                                                                                                                                                                     >  2:482504/1‑289 (MQ=35)
                                                                aTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATATAGCGTGATTGTCGAGGTCGGATAAGGCGTTTATGCACAATCCGACATCAACGCCTGGTGCGACGTTTGACGCGTCGTCACACGCCTACGCCAgcccacgca                                                                                                                                                                                                                    >  2:1262800/1‑281 (MQ=11)
                                                                 cccGACATCAACGCCTGATGGTACGCTTAACGGGTCTGATCAGCCCTACGCCAGACGGAGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAAAAGGCGTTTACGCCGCATCCGACATCAACGCCGGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCaa                                                                                                                                                                                                                   <  1:1555577/288‑1 (MQ=11)
                                                                                        cGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                                                                                                                    >  1:300833/1‑233 (MQ=37)
                                                                                        cGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                                                                                                                    <  2:300833/233‑1 (MQ=37)
                                                                                         ctttaggtggttgtagcAGGCCTGCGCCAGGAGGTGAAAAAGCTTGTTTTAGGGTGAATTGGTAGGGCGAATAGGTCTTCTACAGCGCAGCCGACGTCATCGCCTGATGCGTCTGTTGCTGAGGCTTTTCAGGCCTACGCGCGACAGCGCAATAGCCTGATTGAGCGTGATTTTGTAGGTCGGATAATGCGTTTATGCCGCAGCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCGACGCCAGACAGAGCAATAGCCTGATTTAGCGTGATTTTg                                                                                                                                                                                            <  2:1576588/272‑1 (MQ=2)
                                                                                             agcgagtctagtCAAGCCTACGCCAGACAGCGCAATGGGCTGATTGAGCTTGATTTTGTAGGTCGGAGAAGGCGTTTACACCGCATCAGGCATCAACACCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAAGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGt                                                                                                                                                                                       <  1:83913/278‑1 (MQ=11)
                                                                                             aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATTCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATTCGCCATCCACGCCCGATGCGACGCTTCACGCGTCTTTTCAGGCCTACGCCAGACAGCGCAATCACCTGATTTAGCGTGATTTTGTAgg                                                                                                                                                                                        >  1:624121/1‑288 (MQ=255)
                                                                                                   tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAAGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATa                                                                                                                                                                                 >  1:1160525/1‑289 (MQ=18)
                                                                                                    cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                                                                                                                    <  2:615852/221‑1 (MQ=34)
                                                                                                    cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                                                                                                                    >  1:615852/1‑221 (MQ=34)
                                                                                                        tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACg                                                                                                                                                                                                                                                         <  1:1418987/212‑1 (MQ=34)
                                                                                                        tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACg                                                                                                                                                                                                                                                         >  2:1418987/1‑212 (MQ=34)
                                                                                                            gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                     <  2:1155920/228‑1 (MQ=34)
                                                                                                            gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                     >  1:1155920/1‑228 (MQ=34)
                                                                                                               tACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTacg                                                                                                                                                                      >  1:1076781/1‑286 (MQ=17)
                                                                                                                      gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGACTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAAACACCGGAAAAGCCTGACTTACCGTGATTTTGTAGGTCGGATTAGGCGTTTaccccccacc                                                                                                                                                               >  2:556294/1‑279 (MQ=11)
                                                                                                                           cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTTCTGCGTCTTATCAGGCCTACGCCAGACAGCGCATAAGCCTGATTCAGCCTGATTTTGAAGGTCGGATAAGGCCTTTCCGCAGCATCAGACATCAATCCCTGAGGCGACGCTTCACGAGTCCTAGCAGGCCTCTGCTACCAATCGTCATACACTGATTTCGTGTGCTTTTGTCGGTCGGATacgtacattagcacgtatccgaac                                                                                                                                                          >  2:1165038/1‑265 (MQ=2)
                                                                                                                             cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCg                                                                                                                                                                                                    <  1:1226432/244‑1 (MQ=33)
                                                                                                                                   ccTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATc                                                                                                                                                                                                                                         <  2:1195195/201‑1 (MQ=34)
                                                                                                                                     tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTCATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGTGCAATAGCCTGATTTAGCGTGACTCTGTAGGGCGGATAAGGTGTTGAAGCCGCAGTCCACATCCATGCCTGa                                                                                                                                               >  2:1198312/1‑289 (MQ=11)
                                                                                                                                      gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAACGCGTTTATGCCGCATCCGACATCAACTCCTGATGCGCCGCTTACCGCGTCTTACCAGTCCTACGCTACACCTTGCAATACCCTTCTTCCGCGCGCATTCGTGGGTCGGTTACGGCTTTTACGGCCCATCTGATATCAATTCCTGAt                                                                                                                                              >  2:459340/1‑289 (MQ=11)
                                                                                                                                       aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTa                                                                                                                                                                                          >  2:1226432/1‑244 (MQ=33)
                                                                                                                                             cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGc                                                                                                                                                                                                                               >  1:1195195/1‑201 (MQ=34)
                                                                                                                                                     ttgaggttgtggagggggtgtACGCAGAAACCGACATGAACGCCGGAGGCGTCGCTTGGCTCTTCTTATCAGGCCGACGCGAGTAAGCGAAATAGCCTGATTTAGCGTTATTTTGGAGGTCGGATAAGGCGTTTAAGCCGGTTCCGACATCAACTCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTGAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCCTCCGACATCAATGCCTGATGCGACGCTTGCcgcg                                                                                                                               <  2:368000/269‑1 (MQ=11)
                                                                                                                                                                        ttACGCCGCATCCGACATCAACGTACGATGCGACGCTTGCCGAGTCTTATCAGGACTAAGCAAGACAGGGCAATAGCCTGATTTAGCGTGATTTTGTAGGTAGGAAAAGGCGTTTATGCCGCATCCGACATCAAAGCCTGATTCGACGCTTAACGCCTATTATCATGCCTACGCCAGATAGCGCCATAGCCTGGTTTAGCGTGATTTTGTAGGTCTGTTCAGGCGTTTCAGCCGCATCCCATATTAATTCCTGATGCCATGCCTGACCCGTCCCATCacgacgaatcta                                                                                                            >  2:884826/1‑278 (MQ=2)
                                                                                                                                                                                       cATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                                                                                                                                               <  1:620462/175‑1 (MQ=33)
                                                                                                                                                                                                                                            cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCACACCGCGCAATATCCTGATTTAGCGTGATTTTGTAGCTCGGATAAGGCGTTTATGCCGCATCCGACATCAACCCCTGCTGCGACGCTTACCGCCTCTTTTCAGGCCTACACCAGACAGCGCGATATCATGCATGAGCTGGTTTTCGTAGCTCGGATactcactttccgcccttctcgcga                                         >  2:348960/1‑265 (MQ=11)
                                                                                                                                                                                                                                                      tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCCGAATAGCCTGATTTAGCGTTATTTTGTATGTCGGATAAGGCGTTTACGCCGCATCCGACCTCaatgcctga                              <  2:1556083‑M2/289‑10 (MQ=255)
                                                                                                                                                                                                                                                                       gTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAACTTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgtttgccgcg              <  1:180805‑M2/288‑26 (MQ=255)
                                                                                                                                                                                                                                                                           gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtctta         <  2:577824‑M2/289‑31 (MQ=255)
                                                                                                                                                                                                                                                                             cGGATAAGGCGTTTACGCCCCATCCGACACCAAAGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCTTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttat        >  1:110493‑M2/1‑257 (MQ=255)
                                                                                                                                                                                                                                                                                         ttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttatcaggcc  <  2:118851‑M2/282‑38 (MQ=255)
                                                                                                                                                                                                                                                                                         ttACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttatcaggcc  >  1:118851‑M2/1‑245 (MQ=255)
                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                      
CGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCC  >  NC_000913/4295870‑4296432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: