Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,326,089	T→G	9.1%	intergenic (‑347/+311)	yjdN ← / ← yjdM	metalloprotein superfamily protein/zinc‑ribbon family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,326,089	0	T	G	9.1%	142.2 / 9.8	65	intergenic (‑347/+311)	yjdN/yjdM	metalloprotein superfamily protein/zinc‑ribbon family protein
Reads supporting (aligned to +/- strand):  ref base T (26/31);  new base G (3/4);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01

GCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTAT  >  NC_000913/4325807‑4326376                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                 gcCTGATAAGACGCGCGAGAGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGACACATAAACGCCTTACCCGGACTACGGAGGGAGCGGGAAGTAGTGGGCCTGATAAGACGCCCAAACGT‑CGCCTCAGGCGGAAGGCACCAATGCCGGATGCGGCGTAATAGCATTATACGACACACGGAGGGTGGGGGAACTTGGAGGCCTGATAGGACTGCCAAACGTCGCCTCCAGCAGGCCGCCGCGTGGGCGCGTGCGGCGTAAACGTCTTATTCGGCACACGGATGGttac                                                                                                                                                                                                                                                                                            >  2:209720/1‑286 (MQ=255)         agacgggcaggggTCGGATGAGGCAGTGGGAACTGGTGCCGGAGGGGGCGTGAACGCCTTATCCGGCGTAGGGGGGGTGCGGGAAGTGGTAGGCCGGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTg                                                                                                                                                                                                                                                                                    <  2:1264281/277‑1 (MQ=11)           aCGGGCGAGGGGCGCATCAGGCAGTCGGGACTGTGGCCGGGGGCGGCGTAAAAGCCTTATCCGGCGTACGGGGGGTGCGGGAATGGGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGt                                                                                                                                                                                                                                                                                   <  2:302872/288‑1 (MQ=11)               gcgtgcgtCGCGTCAGGGAGTCGGTACGGTTGCGGGAGGCGGCGTAAGCGCCGTAGCGTGCCGACGGAGGGTGCGGGGATTTGTAGGCCTGATAAGAGGGGGAAGCGT‑GGCATCAGGCAGTCGGCACCATTGCCGGATGAGGCGTAAACGCCTTATCCGGCCTGCGGAGGGTGCGGGAATTTGGAGGCCGGATAAGGCGCGCGAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGc                                                                                                                                                                                                                                                                               <  2:789850/288‑1 (MQ=11)                                  gtggggacggttggccgattggggatAAACACCTTATCCGGCATTAAGAGGGTGCAGGACTTTGTAGGCCTGATAATACGGGCAAGCGT‑CGCATCGGGCAGTTGGCAGCATTACCTGATGCTGCCTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGGATTTGTAGGCCTGATAAGACGGGCAATCGTCGCATCAGGCAGTCGGCACCGTTGCCGGCTGCGGCGTAAACGCCTTATCCGGCCTAGGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGt                                                                                                                                                                                                                                                           <  2:1541971/264‑1 (MQ=11)                                    cGGCACTGTTGCCGGATGCGGAGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATTAGGCGTAAACGCCTTATCCGGCCTACGGAGGGAGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGAAGTCGGGCACGGTGCCGGATGCGGCGTAAACGCCTTATCCGGACAACGGATGGTGCGGGGATTTGTAGGCATGATAAGACGAGCACGCGTc                                                                                                                                                                                                                                                          >  1:556507/1‑288 (MQ=11)                                        aacgtttcaggATGCGGCGTAAACGCCTTATCCGGCCTACGGGGGGTGCGGGAATTTGTAGGCCTGATAGGACGCGCAAGCGT‑CGCAGCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTGATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATc                                                                                                                                                                                                                                                     <  2:1382534/280‑1 (MQ=255)                                          gtttGCCCGATGCGGCGGAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAATGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAg                                                                                                                                                                                                                                                   <  1:1410873/287‑1 (MQ=18)                                           ggtGCCGGTTGCGGCTTAAACGCCTTATCCGGGCTACGGAGGGTGCGGGAATGTGTAGGCCTGATAAGACGGGCAAACGT‑GGCATCAGGCAGTCGGCACCATGGCCGGATGCGGCGTAAACGCCTTATCCGGCCGACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAgg                                                                                                                                                                                                                                                  <  2:744929/287‑1 (MQ=11)                                             tGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCGGGCAGTCGGCCCCATTGCCGGAAGCGGGGTAAACGCCTTATCCGGCCTACCGAGGGCTCCGGCCTTTGTTGGCCTGATAAGACGCGCACGCGTCTCAACCGGCAACAGGCACCCATGCCGGCTTCGCCGTAAAAGCCTTAGCCTGCCTACGGATGGCTCGGGCATTTGTAGTGCCGATAAgcctcatcgagctacaacacggc                                                                                                                                                                                                                                                 >  2:792815/1‑266 (MQ=2)                                                   aTGCGGCGTAAACGCCTTATCCGGCCGACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATGGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTgg                                                                                                                                                                                                                                           <  1:1502856/288‑1 (MQ=37)                                                    tGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCa                                                                                                                                                                                                                                         <  2:1434919/289‑1 (MQ=37)                                                    tGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCa                                                                                                                                                                                                                                         <  1:418420/289‑1 (MQ=21)                                                         cgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTg                                                                                                                                                                                                                                                                                    <  2:1425115/241‑1 (MQ=18)                                                                   ttATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGAt                                                                                                                                                                                                                                                                          >  1:1425115/1‑241 (MQ=18)                                                                     aTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCCGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATAAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGCATTTGGAGGCCTGATAAGACGCGCACGCGTCGCATCGTGCAGTGGGCTCTGTTGCCGTACgcggc                                                                                                                                                                                                                        >  2:651641/1‑289 (MQ=11)                                                                         ggCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTaa                                                                                                                                                                                                                    <  1:18265/289‑1 (MQ=38)                                                                             gtcGGAGGGTGCGGGAGTGTGTAGACCGGATCAGGCGCGCAA‑CGTGGGCATCAGGCGGTGGGAACCATTGCCGGGTGCGGGGGAAAGGCCCTATCCGGGTTAGGGAGGGTTCGGGAATTTGTCGGCCTGATAAGACGCGCAAGCGTCGAATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATTGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGc                                                                                                                                                                                                                <  2:1410812/287‑1 (MQ=11)                                                                                   gtgggCGGGAATGTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                                                                                                                                                           <  1:469356/284‑1 (MQ=38)                                                                                   gggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCTGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                                                                                                                                                           <  1:651641/288‑1 (MQ=38)                                                                                    ggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGGAAACGCCTTATcc                                                                                                                                                                                                         >  1:909614/1‑289 (MQ=38)                                                                                     gTGCGGGAGTTTGTAGTCCTGGTCAGACGCGAAAGCGT‑CCCATCAGGCATTCGGCGCCATTGCCGGATGCGGCTTAAACGCTTTAGCCGGCCTAGGGAGGGTGCGGGAATGTGTAGGCCTGATAAGAGGCGCAAGCGTCGCATCAGGAAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                                                                                                                                                         <  2:1179367/288‑1 (MQ=11)                                                                                              tttGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGa                                                                                                                                                                                                                                                                                         >  1:338085/1‑199 (MQ=37)                                                                                                     gCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                                                                          >  2:726296/1‑271 (MQ=38)                                                                                                     gCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                                                                                                                                                          <  1:726296/271‑1 (MQ=38)                                                                                                        tGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCTAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGTGGGTGCGGGa                                                                                                                                                                                     <  1:209720/289‑1 (MQ=255)                                                                                                        ggATAAGACGCGAAAGCGT‑CGCGTCAGGCAGTCGGCACCATTGCGGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGa                                                                                                                                                                                     <  2:1189879/288‑1 (MQ=255)                                                                                                          aTAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCAGCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTCAACGCCTTATCCGGCCTACGGATGGTGCGGGCATTTGTAGGCCTGATAAGACGAGCAAGCGTCGCATCACGCAGTCGGCACCATTGCCGGGTGCGGCGTAAACGCCGTATCCGGcccagcgagtcacgggtgat                                                                                                                                                                                   >  2:18265/1‑271 (MQ=11)                                                                                                                  cgcAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAg                                                                                                                                                                                                                                                   <  2:1304509/217‑1 (MQ=35)                                                                                                                  cgcAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAg                                                                                                                                                                                                                                                   >  1:1304509/1‑217 (MQ=35)                                                                                                                    cAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTCTCCGGCCTACGGAGGGGGCGGGAATTTGTTgg                                                                                                                                                                            >  1:82353/1‑286 (MQ=255)                                                                                                                              cATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGa                                                                                                                                                                                                                                                                                         >  2:11500/1‑168 (MQ=18)                                                                                                                                     cAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCa                                                                                                                                                            <  1:977525/286‑1 (MQ=38)                                                                                                                                                 ttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGc                                                                                                                                             <  1:991542/289‑1 (MQ=35)                                                                                                                                                      ggAGGCGGGGTAGACGCCTGATCCGGCCTGAGGGGGTTGCGGGGAGGTGTCGGCCGGAGAAGACGGGCAAGCGTCGCATCAGGCAGTCGGCACCGTGGCCGGATGGGGCGTAAACGCCTTATCCGGCCTACGGATGGTACGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTc                                                                                                                                         <  2:574598/288‑1 (MQ=11)                                                                                                                                                         tGCGGCGCAAACGCCTTAGCCGGCGTACGGAGGCGACGAGGATGTGGAGGCCGGAGAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGGTGCCGGAGGCGGCGTAAACGCCTTATCCGGCCTGCGGATGGTGCGGGCAGTTGTAGGCCTGATAAGACGCGGAAGTGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCGACGGAGGGTGCGGGAATTGGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCa                                                                                                                                     <  2:319889/289‑1 (MQ=11)                                                                                                                                                                      gtttggcgggCTTACGGAGGGAGCGGGAATGGGTAGGCCTGATAAGACGGGCAAGCGTCGGATCAGGCAGTCGGCACCGTTGCCGGGTGCGGCGTAACCGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGAt                                                                                                                         <  2:1188273/280‑1 (MQ=11)                                                                                                                                                                        ttATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGCTAAGACGCGCAAGCGTCGCCTCAGGCACTTGGCACTGTTGCCGGATGCGCCGTAAACGCCTTATCCGCCCTACGGAGGGTACGGGAATATGGCGCGCTGACAAGACGCGCAAACGCAGCAGCAggaccgtaggcactctctcgtgatc                                                                                                                       >  2:1547179/1‑265 (MQ=11)                                                                                                                                                                        ttATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTCGGCCTGATAAGACGCGCAAGTGTCGCACCCGGCCTCTGGCACCGTTTCCGGATgcg                                                                                                                      >  2:1131423/1‑289 (MQ=11)                                                                                                                                                                              gggggagggggagagggggAGTTGGTAGGCCTGGGAAGAAGCGCAGGAGTGGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTAACCGGGCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTa                                                                                                                 <  2:772078/272‑1 (MQ=11)                                                                                                                                                                                    cGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATACGGCGTAAACGCCTTATCCGGCGTACGGAGGACGCGGGGATTTGTGGGACTGATTAGACGGCCAAGTTTCGTACCTGGCAGTCggaccgctttcctgaagggggcgtaagcg                                                                                                            >  2:666236/1‑260 (MQ=11)                                                                                                                                                                                            gCGGGGAGTTGTGGGCCTGCTAAGACGCGCAAGGGTCGCATCAGGCAGTGGGCACCGTTGCCGGATGCGGCGGAAACGCCGTGTCCGGGCTACGGATGGTGCGGGAATTTGGAGGCCGGATAAGACGCGCAAGCGTCGCATCAGGGAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGGGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                   <  2:95772/288‑1 (MQ=11)                                                                                                                                                                                             cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATg                                                                                                                                                                                                                            <  1:1096774/166‑1 (MQ=38)                                                                                                                                                                                              gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCCGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGAGGGAATTTGTGGGCCTGATAAGACGCGCAAGCGGAGCATCCGGCGTTTGGCCCCGTTTCCGGATGCCGCGCCAGCGCCTTATCCGGct                                                                                                 >  2:408873/1‑287 (MQ=11)                                                                                                                                                                                                     tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAg                                                                                                                                                                                                                                                                                 <  1:886033/105‑1 (MQ=32)                                                                                                                                                                                                     tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCa                                                                                                                                                                                                                                         <  1:875079/145‑1 (MQ=37)                                                                                                                                                                                                       tAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAAc                                                                                                                                                                                                                  >  2:1096774/1‑166 (MQ=38)                                                                                                                                                                                                          gCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGa                                                                                                                                                                                                                                                                                         <  2:322146/92‑1 (MQ=33)                                                                                                                                                                                                            cTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGAGGGTGCggg                                                                                 >  1:1425517/1‑289 (MQ=38)                                                                                                                                                                                                               attaGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTAACCGTCCTACGGATGGTGCGGGAAGTTGTAGGACTGATagt                                                                                                                                                                                                                                                                       >  2:886033/4‑103 (MQ=11)                                                                                                                                                                                                               aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCgg                                                                                                                                                                                                                               >  2:875079/1‑145 (MQ=39)                                                                                                                                                                                                                     cgcgCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAAttt                                                                                                                                                                                                                                                                                     >  1:974859/1‑85 (MQ=33)                                                                                                                                                                                                                     cgcgCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAAttt                                                                                                                                                                                                                                                                                     <  2:974859/85‑1 (MQ=33)                                                                                                                                                                                                                         cAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGGGCGGGAATTTGTAGGCTTGATAAGACTGGCAAGCCTCGCATCAGGCAGTCGGCAACGTTGCCGGATGCGGCGTACACGCACTACCCGGCCTAACGGTGGGTCCggacattgggacgtg                                                                     >  2:30073/1‑276 (MQ=11)                                                                                                                                                                                                                                                     ttGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCAGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGACCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGATGGTGCGGGAATTTGTAGGCATGACAAGACGCGCACGCATCGCACCAAGc                                        >  1:777848/1‑289 (MQ=255)                                                                                                                                                                                                                                                          ggATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGGAAACGCCTTATCCGGCCT‑ACGGAGGGTGCGGGAATTTGTAGGGCTGATACGACCCGCACGCGTCGCACCAGGCATCTg                                   >  2:1580758/1‑289 (MQ=255)                                                                                                                                                                                                                                                             tGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCa                                                                                                                                                                                                                                                                <  1:1390200/66‑1 (MQ=35)                                                                                                                                                                                                                                                                         gCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGCTGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAAACGCACAAGCGTCCAATCAGGCAGTCACCACCGTTTCCGGATGCGGCGTACACGCCTTATCCGGCCT‑ACGGAGGGTGCGGCACTTTTTTGTCCTGATTACACGCGCAATCGCCGCACCAGGCATCAGCAACCATTGcccgca                    >  2:988596/1‑285 (MQ=11)                                                                                                                                                                                                                                                                                 gggCGTAGGGAGGGTGCGGGTATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGTACCGTTGCCGGATGCGGCGTAAACGCCTTAGCCGGCCT‑ACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGcgcg            <  1:599905/288‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gCCTAGGGAAGGGGCGGGAATTTGGAGGCCTGAGAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTAGCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGa           <  2:1010971/288‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCa                                                                                                                                                                                                                                         >  2:1451461/1‑67 (MQ=40)                                                                                                                                                                                                                                                                                   gCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCa                                                                                                                                                                                                                                         <  1:1451461/67‑1 (MQ=40)                                                                                                                                                                                                                                                                                   gCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGAGGGTGCGGGAATTTGTAGGCCTGAACAGGCGCGCAAGTGGCGCATCCGGCATCCGGCCCCCTTGCCGGCTGCGGCGCGa           >  1:235635/1‑288 (MQ=14)                                                                                                                                                                                                                                                                                     cTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTTGCACCGTTGACTGATGCGGCGCGAgc         >  1:450995/1‑288 (MQ=255)                                                                                                                                                                                                                                                                                        cGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTTCCGGATGCGGCGCGAGCGCCt     >  1:609570/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                            tGGTGCGGGAATTGCGAGGCTTGCAAAGACGCGCAAGGGTGGCATCAGGCAGTCGGAACTGTTGCCGAATGCGGCGTAAACGCCTTATCGGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGTTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTAt  <  2:1518088/288‑1 (MQ=14)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                 GCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGT‑CGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCT‑ACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATCTGGCACCGTTGCCGGATGCGGCGCGAGCGCCTTAT  >  NC_000913/4325807‑4326376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑