mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.32.0 revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | pgi-12-4_S25_L001_R1_001.good.fq | 1,590,487 | 422,537,732 | 100.0% | 265.7 bases | 289 bases | 99.7% |
| errors | pgi-12-4_S25_L001_R2_001.good.fq | 1,590,487 | 423,287,037 | 100.0% | 266.1 bases | 289 bases | 94.8% |
| total | 3,180,974 | 845,824,769 | 100.0% | 265.9 bases | 289 bases | 97.2% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,653 | 172.7 | 4.2 | 100.0% | Escherichia coli str. K-12 sbstr. MG1655, complete genome. Live strain from Systems Biology Research Group |
| total | 4,641,653 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 9852 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 577 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.073 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.80558 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | OFF |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.025 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.2.8 |
| R | 3.3.1 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 20:16:24 19 Mar 2018 | 20:17:11 19 Mar 2018 | 47 seconds |
| Read alignment to reference genome | 20:17:12 19 Mar 2018 | 20:21:31 19 Mar 2018 | 4 minutes 19 seconds |
| Preprocessing alignments for candidate junction identification | 20:21:31 19 Mar 2018 | 20:22:30 19 Mar 2018 | 59 seconds |
| Preliminary analysis of coverage distribution | 20:22:30 19 Mar 2018 | 20:24:40 19 Mar 2018 | 2 minutes 10 seconds |
| Identifying junction candidates | 20:24:40 19 Mar 2018 | 20:24:51 19 Mar 2018 | 11 seconds |
| Re-alignment to junction candidates | 20:24:51 19 Mar 2018 | 20:25:45 19 Mar 2018 | 54 seconds |
| Resolving best read alignments | 20:25:45 19 Mar 2018 | 20:27:24 19 Mar 2018 | 1 minute 39 seconds |
| Creating BAM files | 20:27:24 19 Mar 2018 | 20:29:05 19 Mar 2018 | 1 minute 41 seconds |
| Tabulating error counts | 20:29:05 19 Mar 2018 | 20:30:19 19 Mar 2018 | 1 minute 14 seconds |
| Re-calibrating base error rates | 20:30:19 19 Mar 2018 | 20:30:20 19 Mar 2018 | 1 second |
| Examining read alignment evidence | 20:30:20 19 Mar 2018 | 22:17:14 19 Mar 2018 | 1 hour 46 minutes 54 seconds |
| Polymorphism statistics | 22:17:14 19 Mar 2018 | 22:17:14 19 Mar 2018 | 0 seconds |
| Output | 22:17:14 19 Mar 2018 | 22:17:46 19 Mar 2018 | 32 seconds |
| Total | 2 hours 1 minute 21 seconds | ||
