Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	4,204,931:1	(CAC)2→3	3.2%	coding (1000/1290 nt)	purD ←	phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4204925	150 (1.200)	5 (0.040) +CAC	3/492	NT	3.2%	coding (1006/1290 nt)	purD	phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase
	?	NC_000913	4204926 =	150 (1.170)					coding (1005/1290 nt)	purD	phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase

GCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCACAGTACGCGCCCGCCGTTGGTCACTACCTGCTCGTCATCCGCCAGTTTTGTGCCCGCGTGGAACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCACCCGGATATCCACCCGCAGCCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4204665‑4204925 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGG  >  NC_000913/4204926‑4205017                                                                                                                                                                                                                                                                      |||                                                                                             GCGCCCGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGCCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGCATTACCCAGCGCGGTGACGCCCCGTACGCGCCCGCCGTTGGTCACTACCTTCTCGTCATCCGCCAGTTTTGTGCCCGCGTGGAACACTTTCCCGCCTCCCACTTCTTCCAACCGCAGCCCGTGGACCACTTCACCGGTCCGGTAATCCCCCGGAAACCCCCCCCCCACCATCACCACCCCGCCagaagccccttcatc                                                                      >  2:139766/1‑273                                                                                                    CGCGGTGACGCACAGTACGCGCCCGCCGTTGGTCACTACCTGCTCGTCATCCGCCAGTTTTGTGCCCGCGTGGAACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGG  >  1:160693/1‑257                                                                                                    CGCGGTGACGCACAGTACGCGCCCGCCGTTGGTCACTACCTGCTCGTCATCCGCCAGTTTTGTGCCCGCGTGGAACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCACCACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGG  <  2:160693/257‑1                                                                                                                                        TACCTGCTCGTCATCCGCCAGTTTTGTGCCCGCGTGGAACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCAC                                                                                              <  1:374260/129‑1                                                                                                                                        TACCTGCTCGTCATCCGCCAGTTTTGTGCCCGCGTGGAACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCACCCGGATATCCACCCGCAGCCATCAC                                                                                              >  2:374260/1‑129                                                                                                                                                                                                                                                                      |||                                                                                             GCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCACAGTACGCGCCCGCCGTTGGTCACTACCTGCTCGTCATCCGCCAGTTTTGTGCCCGCGTGGAACACTTTGCCGCCTGCCACTTCTTCCAGCGGCAGGCCGTGGATCACGTCACCGGTGCGGTAATCACCCGGATATCCACCCGCAGCCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4204665‑4204925 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CACCACGCCGAGAGAAGCGCGTTCATCCCACTCGGACGTTTTCTCGTCCAGTTTGCTTTCACAGGCCGCCAGGCAGAGCTCAACCAGATCGG  >  NC_000913/4204926‑4205017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.